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product-image-AAA285109_FCM11.jpg FCM/FACS (Flow Cytometry) (ADFP Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Rabbit anti-Human ADFP Polyclonal Antibody | anti-PLIN2 antibody

ADFP Antibody (Center)

Gene Names
PLIN2; ADFP; ADRP
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ADFP, Antibody; ADFP Antibody (Center); Perilipin-2; Adipophilin; Adipose differentiation-related protein; ADRP; PLIN2; ADFP; anti-PLIN2 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ADFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human ADFP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
186-214
Sequence Length
437
Applicable Applications for anti-PLIN2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(ADFP Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA285109_FCM11.jpg FCM/FACS (Flow Cytometry) (ADFP Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(ADFP Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADFP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

product-image-AAA285109_IHC13.jpg IHC (Immunohiostchemistry) (ADFP Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADFP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of ADFP Antibody (Center) in Jurkat cell line lysates (35ug/lane).ADFP (arrow) was detected using the purified Pab.)

product-image-AAA285109_WB15.jpg WB (Western Blot) (Western blot analysis of ADFP Antibody (Center) in Jurkat cell line lysates (35ug/lane).ADFP (arrow) was detected using the purified Pab.)
Related Product Information for anti-PLIN2 antibody
Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation.
Product Categories/Family for anti-PLIN2 antibody
References
Kimmel, A.R., et al. J. Lipid Res. 51(3):468-471(2010)
Kotokorpi, P., et al. Mol. Pharmacol. 77(1):79-86(2010)
Minnaard, R., et al. J. Clin. Endocrinol. Metab. 94(10):4077-4085(2009)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
123
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48075
NCBI Official Full Name
perilipin-2
NCBI Official Synonym Full Names
perilipin 2
NCBI Official Symbol
PLIN2
NCBI Official Synonym Symbols
ADFP; ADRP
NCBI Protein Information
perilipin-2
UniProt Protein Name
Perilipin-2
UniProt Gene Name
PLIN2
UniProt Synonym Gene Names
ADFP; ADRP
UniProt Entry Name
PLIN2_HUMAN

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Product Notes

The PLIN2 plin2 (Catalog #AAA285109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 186-214. The ADFP Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADFP can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PLIN2 plin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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