FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of human KIT (CD117) expression on TF-1 cells. TF-1 cells were stained with PE-conjugated anti-Human KIT (CD117). The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.Flow Cytometry was performed on a BD FACSCalibur flow cytometry system.)
Rabbit c-Kit Monoclonal Antibody | anti-KIT antibody
Recombinant Anti-c-Kit Antibody (PE), Rabbit Monoclonal
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Applications
Flow Cytometry, Functional Assay
Synonyms
c-Kit, Antibody; Recombinant Anti-c-Kit Antibody (PE), Rabbit Monoclonal; c-Kit/CD117 Antibody (PE), Rabbit MAb; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; Anti-C-Kit Antibody; Anti-CD117 Antibody; Anti-PBT Antibody; Anti-SCFR Antibody; anti-KIT antibody
Host
Rabbit
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
007
Specificity
Human c-Kit/CD117
Form/Format
PBS solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-KIT antibody
FCM/FACS (Flow Cytometry)
Immunogen
Recombinant Human c-Kit/CD117 protein
Reagents
PE-conjugated Rabbit monoclonal antibody
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human c-Kit/CD117 (rh c-Kit/CD117; P10721-2; Met1-Thr516) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Preparation and Storage
This antibody can be stored at 2-8°C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of human KIT (CD117) expression on TF-1 cells. TF-1 cells were stained with PE-conjugated anti-Human KIT (CD117). The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.Flow Cytometry was performed on a BD FACSCalibur flow cytometry system.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of human KIT (CD117) expression on TF-1 cells. TF-1 cells were stained with PE-conjugated anti-Human KIT (CD117). The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.Flow Cytometry was performed on a BD FACSCalibur flow cytometry system.)
Product Categories/Family for anti-KIT antibody
References
Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
mast/stem cell growth factor receptor Kit isoform 1
NCBI Official Synonym Full Names
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
NCBI Official Symbol
KIT
NCBI Official Synonym Symbols
PBT; SCFR; C-Kit; CD117
NCBI Protein Information
mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like
UniProt Protein Name
Mast/stem cell growth factor receptor Kit
UniProt Gene Name
KIT
UniProt Synonym Gene Names
SCFR; SCFR; PBT
UniProt Entry Name
KIT_HUMAN
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Product Notes
The KIT kit (Catalog #AAA255502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's c-Kit can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the KIT kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Kit, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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