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FCM/FACS (Flow Cytometry)
(KIT Antibody (N-term D121) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse KIT Polyclonal Antibody | anti-KIT antibody
KIT Antibody (N-term D121)
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
KIT, Antibody; KIT Antibody (N-term D121); Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117; KIT; anti-KIT antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human KIT.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
106-135
Sequence Length
413
Applicable Applications for anti-KIT antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(KIT Antibody (N-term D121) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(KIT Antibody (N-term D121) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemisry)
(KIT Antibody (N-term D121) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KIT Antibody (N-term D121) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(KIT Antibody (N-term D121) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KIT Antibody (N-term D121) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(KIT Antibody (N-term D121) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).)
WB (Western Blot)
(KIT Antibody (N-term D121) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCI-H460 cells using KIT Antibody (N-term D121)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCI-H460 cells using KIT Antibody (N-term D121)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-KIT antibody
KIT is the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
Product Categories/Family for anti-KIT antibody
References
Wardelmann, E., et al., Int. J. Cancer 106(6):887-895 (2003). Lennartsson, J., et al., Exp. Cell Res. 288(1):110-118 (2003). Sakuma, Y., et al., Cancer Sci 94(6):486-491 (2003). Araki, K., et al., Lung Cancer 40(2):173-180 (2003). Voytyuk, O., et al., J. Biol. Chem. 278(11):9159-9166 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109865
NCBI Official Full Name
mast/stem cell growth factor receptor Kit isoform 1
NCBI Official Synonym Full Names
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
NCBI Official Symbol
KIT
NCBI Official Synonym Symbols
PBT; SCFR; C-Kit; CD117
NCBI Protein Information
mast/stem cell growth factor receptor Kit
UniProt Protein Name
Mast/stem cell growth factor receptor Kit
UniProt Gene Name
KIT
UniProt Synonym Gene Names
SCFR; SCFR; PBT
UniProt Entry Name
KIT_HUMAN
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Product Notes
The KIT kit (Catalog #AAA286639) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 106-135. The KIT Antibody (N-term D121) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KIT can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KIT kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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