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product-image-AAA17955_APP3.jpg Application Data

Mouse anti-Human CD133 Monoclonal Antibody | anti-CD133 antibody

CD133 Antibody

Gene Names
PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, ELISA
Synonyms
CD133, Antibody; CD133 Antibody; RP41; AC133; CD133; MCDR2; STGD4; CORD12; anti-CD133 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F10
Specificity
Recognizes endogenous levels of TAU (pS579) protein.
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
856
Applicable Applications for anti-CD133 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC), ELISA (EIA)

Not yet tested in other applications.
Application Notes
IHC: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000
Immunogen
Synthesized peptide of human CD133 (AA: 20-36, GGQPSSTDAPKAWNYELc)
Preparation and Storage
Store at 4°C, for long term storage, store at -20°C.

Application Data

product-image-AAA17955_APP3.jpg Application Data

IHC (Immunohistochemistry)

(Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and human esophageal cancer tissues (right) using CD133 mouse mAb with DAB staining.)

product-image-AAA17955_IHC2.jpg IHC (Immunohistochemistry) (Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and human esophageal cancer tissues (right) using CD133 mouse mAb with DAB staining.)

FCM (Flow Cytometry)

(Figure 2: Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).)

product-image-AAA17955_FCM.jpg FCM (Flow Cytometry) (Figure 2: Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).)
Related Product Information for anti-CD133 antibody
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
References
EFFECT OF HELICOBACTER PYLORI INFECTION ON CANCER STEM CELL MARKER (CD133) IN COLORECTAL CANCER. Mayadah Mohammed Ridha Abdul-Razzaq, Zainab Fadhel Ashoor, Wasan Abdul-ilah Bakir (2017)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
133kDa
NCBI Official Full Name
prominin-1 isoform 2
NCBI Official Synonym Full Names
prominin 1
NCBI Official Symbol
PROM1
NCBI Official Synonym Symbols
RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
NCBI Protein Information
prominin-1
UniProt Protein Name
Prominin-1
UniProt Gene Name
PROM1
UniProt Synonym Gene Names
PROML1

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Product Notes

The CD133 prom1 (Catalog #AAA17955) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD133 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD133 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC), ELISA (EIA) Not yet tested in other applications. IHC: 1:200-1:1000 FC: 1:200-1:400 ELISA: 1:10000. Researchers should empirically determine the suitability of the CD133 prom1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD133, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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