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WB (Western Blot)
(Western blot analysis of extracts from 293, using CD133 Antibody. The lane on the left was treated with blockingpeptide.)
Rabbit CD133 Polyclonal Antibody | anti-CD133 antibody
CD133 Antibody
Gene Names
PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
CD133, Antibody; CD133 Antibody; AC133; Antigen AC133; CD133; CORD12; Hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061; OTTHUMP00000217744; OTTHUMP00000217745; OTTHUMP00000217746; PROM1; PROM1_HUMAN; Prominin I; Prominin like 1; Prominin like protein 1 precursor; Prominin mouse like 1; Prominin-1; Prominin-like protein 1; Prominin1; PROML1; RP41; STGD4; anti-CD133 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CD133 antibody detects endogenous levels of total CD133
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
856
Applicable Applications for anti-CD133 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CD133, corresponding to a region within N-terminal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from 293, using CD133 Antibody. The lane on the left was treated with blockingpeptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using CD133 Antibody. The lane on the left was treated with blockingpeptide.)
IHC (Immunohistochemisry)
(CD133 Antibody for IHC in human testis.)
IHC (Immunohistochemisry)
(CD133 Antibody for IHC in human testis.)
WB (Western Blot)
(Western blot analysis of CD133 expression in SW620 cells, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of CD133 expression in SW620 cells, The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence)
(AAA321807 staining Caco 2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA321807 staining Caco 2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-CD133 antibody
Description: CD133, originally known as AC133. CD133 is a glycoprotein also known in humans and rodents as Prominin 1 (PROM1). Currently the function of CD133 is unknown. It is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. CD133 is expressed in hematopoietic stem cells.
Function: May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Subunit Structure: Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B.
Post-translational Modifications: Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
Similarity: Belongs to the prominin family.
Function: May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Subunit Structure: Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B.
Post-translational Modifications: Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
Similarity: Belongs to the prominin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97 kDa
NCBI Official Full Name
prominin-1 isoform 2
NCBI Official Synonym Full Names
prominin 1
NCBI Official Symbol
PROM1
NCBI Official Synonym Symbols
RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
NCBI Protein Information
prominin-1
UniProt Protein Name
Prominin-1
UniProt Gene Name
PROM1
UniProt Synonym Gene Names
PROML1
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Product Notes
The CD133 prom1 (Catalog #AAA321807) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD133 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD133 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD133 prom1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD133, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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