IHC (Immunohistochemistry)
(Immunohistochemistry: Human kidney tissue stained with rabbit Anti-CD133 antibody (Cat# AAA14080) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Rabbit CD133 Antibody | anti-CD133 antibody
Rabbit anti CD133 antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, ELISA, Immunoprecipitation
Purity
The Rabbit IgG is purified by Epitope affinity purification
Synonyms
CD133; N/A; Rabbit anti CD133 antibody; Rabbit anti-CD133; AC133; Prominin 1; anti-CD133 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~97 kDa of mouse or rat CD133 protein from mouse embryonic stem cells lystes by Western blot. This antibody reacts with human, rat and mouse. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Epitope affinity purification
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-CD133 antibody
Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB), Flow Cytometry (FC), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
IHC: 2-10 ug/ml
Flow cytometry: 0.5-5 ug/106 cells
Optimal dilutions should be determined by researchers for the specific applications.
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
IHC: 2-10 ug/ml
Flow cytometry: 0.5-5 ug/106 cells
Optimal dilutions should be determined by researchers for the specific applications.
Antigen Preparation
A 18aa synthetic peptide corresponding to C-terminus human CD133.
Positive Control
Kidney tissue
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C to 8°C for 3 months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
IHC (Immunohistochemistry)
(Immunohistochemistry: Human kidney tissue stained with rabbit Anti-CD133 antibody (Cat# AAA14080) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
IHC (Immunohistochemistry)
(Immunohistochemistry: Human kidney tissue stained with rabbit Anti-CD133 antibody (Cat# AAA14080) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
WB (Western Blot)
(Western Blot: The tissue lysate derived from mouse kidney was immunoprobed by Rabbit anti-CD133 (Cat# AAA14080) at 1:500. An immunoreactive band is observed around ~117kDa. This band was depleted by pre-incubation with immunizing peptide.)
WB (Western Blot)
(Western Blot: The tissue lysate derived from mouse kidney was immunoprobed by Rabbit anti-CD133 (Cat# AAA14080) at 1:500. An immunoreactive band is observed around ~117kDa. This band was depleted by pre-incubation with immunizing peptide.)
Related Product Information for anti-CD133 antibody
Background: CD133, originally known as AC133, is a glycoprotein, also known in humans and rodents as Prominin 1 (PROM1). CD133 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. CD133 is expressed in hemtopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, virous pediatric brain tumors as well as adult kidney, mammary glands, trachea salivary glands, placenta, digestive tract, testes, and some other cell types.
References
Role of Rac1 pathway in epithelial-to-mesenchymal transition and cancer stem-like cell phenotypes in gastric adenocarcinoma. Changhwan Yoon, Soo-Jeong Cho, Kevin K Chang, Do Joong Park, Sandra Ryeom and Sam S. Yoon (2017)
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Product Notes
The CD133 (Catalog #AAA14080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CD133 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD133 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB), Flow Cytometry (FC), ELISA (EIA), Immunoprecipitation (IP). Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml IHC: 2-10 ug/ml Flow cytometry: 0.5-5 ug/106 cells Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the CD133 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD133, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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