Mouse anti-Human CD133 Antibody | anti-CD133 antibody

Anti-Hu CD133 PE-Cy7

Cat. Num. AAA129377

• Gene Names: PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD133; N/A; Anti-Hu CD133 PE-Cy7; prominin 1; Prominin 1, PROM1, AC133, PROML1, STGD4; anti-CD133 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG2b
• Clone Number: 293C3
• Specificity: The mouse monoclonal antibody 293C3 recognizes the extracellular epitope 2 on human CD133 (CD133/2), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of human renal progenitors.
• Purity/Purification: Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Applicable Applications for anti-CD133 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: human CD133 transfectants
• Conjugation: PE-Cy7
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD34 positive CD45dim hematopoietic stem cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD133 (293C3) PE-Cy7 antibody (4ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells stained using anti-human CD45 (MEM-28) Pacific Blue antibody (4ul reagent/100ul of peripheral whole blood) and anti-human CD133 (293C3) PE-Cy7 antibody (4ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells stained using anti-human CD34 (581) APC antibody (10ul reagent/100ul of peripheral whole blood) and anti-human CD133 (293C3) PE-Cy7 antibody (4ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD133 (293C3) PE-Cy7 antibody (4ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD133 antibody

CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.

References

*Hermansen SK, Christensen KG, Jensen SS, Kristensen BW: Inconsistent immunohistochemical expression patterns of four different CD133 antibody clones in glioblastoma. J Histochem Cytochem. 2011 Apr;59(4):391-407., URL: https://www.ncbi.nlm.nih.gov/pubmed/21411810 ,*Angelotti ML, Lazzeri E, Lasagni L, Romagnani P: Only anti-CD133 antibodies recognizing the CD133/1 or the CD133/2 epitopes can identify human renal progenitors. Kidney Int. 2010 Sep;78(6):620-1, URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=only+anti-cd133+antibodies+recognizing ,*Feng HL, Liu YQ, Yang LJ, Bian XC, Yang ZL, Gu B, Zhang H, Wang CJ, Su XL, Zhao XM: Expression of CD133 correlates with differentiation of human colon cancer cells. Cancer Biol Ther. 2010 Feb;9(3):216-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=feng+hl+yang+zl+zhang+h ,*Zhang M, Liu Y, Feng H, Bian X, Zhao W, Yang Z, Gu B, Li Z, Liu Y: CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2. Am J Pathol. 2013 Feb;182(2):565-76. , URL: https://www.ncbi.nlm.nih.gov/pubmed/23195431 ,*Barrantes-Freer A, Renovanz M, Eich M, Braukmann A, Sprang B, Spirin P, Pardo LA, Giese A, Kim EL: CD133 expression is not synonymous to immunoreactivity for AC133 and fluctuates throughout the cell cycle in glioma stem-like cells. PLoS One. 2015 Jun 18;10(6):e0130519., URL: https://pubmed.ncbi.nlm.nih.gov/26086074/,*Lardon J, Corbeil D, Huttner WB, Ling Z, Bouwens L: Stem cell marker prominin-1/AC133 is expressed in duct cells of the adult human pancreas. Pancreas. 2008 Jan;36(1):e1-6. doi: 10.1097/mpa.0b013e318149f2dc., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=lardon+j+corbeil+d ,*Richardson GD, Robson CN, Lang SH, Neal DE, Maitland NJ, Collins AT: CD133, a novel marker for human prostatic epithelial stem cells. J Cell Sci. 2004 Jul 15;117(Pt 16):3539-45., URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=richardson+gd+robson+cn,

NCBI and Uniprot Product Information

• NCBI GI #: 13124442
• NCBI GeneID: 8842
• UniProt Accession #: O43490; Q6SV49; Q6SV50; Q6SV51; Q6SV52; Q6SV53; Q96EN6
• Molecular Weight: 97kDa
• NCBI Official Full Name: Prominin-1
• NCBI Official Synonym Full Names: prominin 1
• NCBI Official Symbol: PROM1
• NCBI Official Synonym Symbols: RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
• NCBI Protein Information: prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen
• UniProt Protein Name: Prominin-1
• UniProt Gene Name: PROM1
• UniProt Synonym Gene Names: PROML1
• UniProt Entry Name: PROM1_HUMAN