IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and human esophageal cancer tissues (right) using CD133 mouse mAb with DAB staining.)
Mouse anti-Human CD133 Monoclonal Antibody | anti-CD133 antibody
CD133 Antibody
Gene Names
PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry
Purity
Affinity-Chromatography
Synonyms
CD133, Antibody; CD133 Antibody; AC133; Antigen AC133; CD133; CORD12; Hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061; OTTHUMP00000217744; OTTHUMP00000217745; OTTHUMP00000217746; PROM1; PROM1_HUMAN; Prominin I; Prominin like 1; Prominin like protein 1 precursor; Prominin mouse like 1; Prominin-1; Prominin-like protein 1; Prominin1; PROML1; RP41; STGD4; anti-CD133 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
CD133 antibody detects endogenous levels of total CD133
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Sequence Length
856
Applicable Applications for anti-CD133 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry)
Immunogen
Purified recombinant fragment of human CD133 expressed in E. Coli
Preparation and Storage
Store at -20 degrees C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and human esophageal cancer tissues (right) using CD133 mouse mAb with DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and human esophageal cancer tissues (right) using CD133 mouse mAb with DAB staining.)
Related Product Information for anti-CD133 antibody
Description: This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Function: May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Subunit Structure: Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B.
Post-translational Modifications: Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
Similarity: Belongs to the prominin family.
Function: May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Subunit Structure: Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B.
Post-translational Modifications: Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
Similarity: Belongs to the prominin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 133 kDa
Predicted: 98 kDa
Predicted: 98 kDa
NCBI Official Full Name
prominin-1 isoform 2
NCBI Official Synonym Full Names
prominin 1
NCBI Official Symbol
PROM1
NCBI Official Synonym Symbols
RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
NCBI Protein Information
prominin-1
UniProt Protein Name
Prominin-1
UniProt Gene Name
PROM1
UniProt Synonym Gene Names
PROML1
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Product Notes
The CD133 prom1 (Catalog #AAA322675) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD133 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD133 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD133 prom1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD133, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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