Rat anti-Mouse CD2 Monoclonal Antibody | anti-CD2 antibody

Anti-Ms CD2 FITC

Cat. Num. AAA129143

• Gene Names: Cd2; Ly37; LFA-2; Ly-37
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD2, Antibody; Anti-Ms CD2 FITC; LFA-2; Ly37; Ly-37; CD2 molecule; anti-CD2 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2b lambda
• Clone Number: RM2-5
• Specificity: The rat monoclonal antibody RM2-5 recognizes an extracellular epitope of CD2, a 50kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.
• Purity/Purification: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-CD2 antibody: FCM/FACS (Flow Cytometry)
• Conjugation: FITC
• Immunogen: murine thymocytes
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Related Product Information for anti-CD2 antibody

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

References

*Wallin J, Eibel H, Neubüser A, Wilting J, Koseki H, Balling R: Pax1 is expressed during development of the thymus epithelium and is required for normal T-cell maturation. Development. 1996 Jan;122(1):23-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=wallin+j+,*Altin JG, Pagler EB, Parish CR: Evidence for cell surface association of CD2 and LFA-1 (CD11a/CD18) on T lymphocytes. Eur J Immunol. 1994 Feb;24(2):450-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/7905418,*Sasada T, Reinherz EL: A critical role for CD2 in both thymic selection events and mature T cell Functional Application (FUNC)tion. J Immunol. 2001 Feb 15;166(4):2394-403., URL: https://www.ncbi.nlm.nih.gov/pubmed/11160298,*Cho HJ, Edmondson SG, Miller AD, Sellars M, Alexander ST, Somersan S, Punt JA: Cutting edge: identification of the targets of clonal deletion in an unmanipulated thymus. J Immunol. 2003 Jan 1;170(1):10-3., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=edmondso,*Fassett MS, Jiang W, D'Alise AM, Mathis D, Benoist C: Nuclear receptor Nr4a1 modulates both regulatory T-cell (Treg) differentiation and clonal deletion. Proc Natl Acad Sci U S A. 2012 Mar 6; 109(10):3891-6., URL: https://www.pnas.org/content/109/10/3891.shot,*Milstein O, Tseng SY, Starr T, Llodra J, Nans A, Liu M, Wild MK, van der Merwe PA, Stokes DL, Reisner Y, Dustin ML: Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 2008 Dec 5;283(49):34414-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=milstein+,*Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wülfing C, Schatzle J, Mathew PA, Sharpe AH, Kumar V: Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector Functional Application (FUNC)tions. Blood. 2006 Apr 15;107(8):3181-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/term=forman+jp,*Cibotti R, Bhandoola A, Guinter TI, Sharrow SO, Singer A: CD8 coreceptor extinction in signaled CD4(+)CD8(+) thymocytes: coordinate roles for both transcriptional and posttranscriptional regulatory mechanisms in developing thymocytes. Mol Cell Biol. 2000 Jun;20(11):3852-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/10805728,*Izon DJ, Punt JA, Xu L, Karnell FG, Allman D, Myung PS, Boerth NJ, Pui JC, Koretzky GA, Pear WS: Notch1 regulates maturation of CD4+ and CD8+ thymocytes by modulating TCR signal strength. Immunity. 2001 Mar;14(3):253-64., URL: http://www.ncbi.nlm.nih.gov/pubmed/11290335

NCBI and Uniprot Product Information

• NCBI GI #: 115976
• NCBI GeneID: 12481
• UniProt Accession #: P08920; Q61394
• Molecular Weight: 38,415 Da
• NCBI Official Full Name: T-cell surface antigen CD2
• NCBI Official Synonym Full Names: CD2 antigen
• NCBI Official Symbol: Cd2
• NCBI Official Synonym Symbols: Ly37; LFA-2; Ly-37
• NCBI Protein Information: T-cell surface antigen CD2; LFA-3 receptor; lymphocyte antigen 37; T-cell surface antigen T11/Leu-5
• UniProt Protein Name: T-cell surface antigen CD2
• UniProt Gene Name: Cd2
• UniProt Synonym Gene Names: Ly-37; Ly-37
• UniProt Entry Name: CD2_MOUSE