Mouse Cruciform DNA Monoclonal Antibody | anti-CRP1 antibody

Cruciform DNA Mouse Monoclonal Antibody (2D3)

Cat. Num. AAA14615

• Applications: Western Blot, Gel Super Shift Assay, Immunofluorescence
• Synonyms: Cruciform DNA, Antibody; Cruciform DNA Mouse Monoclonal Antibody (2D3); anti-CRP1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: lgG1
• Clone Number: 2D3
• Specificity: Specific to stem loop structure of Cruciform DNA
• Form/Format: Lyophilized protein G purified in PBS pH7.4
• Sequence Length: 465

• Applicable Applications for anti-CRP1 antibody: Western Blot (WB), Gel Shift (EMSA), Immunofluorescence (IF)
• Application Notes: If reconstituted with deionized water in 100 uL: ; EMSA: 1-10 µg per sample.; IF: 1:200.; Optimal dilution has to be determined by the user.
• Immunogen: Heteroduplex (cruciform) DNA
• Target: Cruciform DNA
• Preparation and Storage: Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay

Related Product Information for anti-CRP1 antibody

Inverted repeat sequences are known to form cruciform structures in negatively supercoiled DNA. They are widespread in the genomes of both eukaryotes and prokaryotes, occurring more often than expected among random sequences. In some bacteria, extrusion of cruciform DNA is required for initiation of replication and transcription, though certain inverted repeat sequences are unstable when subcloned into bacteria. Because such structures might impair DNA replication fidelity, the role of inverted repeats in mutagenesis and in human diseases has been widely studied.

References

1. Callejo M-The 14-3-3 protein homologues from Saccharomyces cerevisiae, Bmh1 p and Bmh2p, have cruciform DNA-binding activity and associate in vivo with ARS307. 2. Frappier L-Monoclonal antibodies to cruciform DNA structures. 3. Frappier L-Characterization of the binding specificity of two anticruciform DNA monoclonal antibodies. 4. Kim EL-Cruciform-extruding regulatory element controls cell-specific activity of the tyrosine hydroxylase gene promoter. 5. Tam M-Slipped (CTG).(CAG) repeats of the myotonic dystrophy locus: surface probing with anti-DNA antibodies.

NCBI and Uniprot Product Information

• NCBI GI #: 151944092
• NCBI Official Full Name: Cruciform DNA binding protein
• UniProt Protein Name: Cruciform DNA-recognizing protein 1
• UniProt Gene Name: CRP1