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FCM (Flow Cytometry)
(Figure-6: Epitope binding study by flow cytometric analysis. MCF-7 cells expressing HER2 antigen were treated with Herceptin and Samceptin (2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
ErbB2/HER2 recombinant antibody
Recombinant anti-human ErbB2/HER2 Antibody (Trastuzumab) FITC Conjugated
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human
Applications
Functional Assay, Flow Cytometry, Functional Assay
Purity
Purity: >98.0% as determined by SEC-HPLC & SDS-PAGE
Synonyms
ErbB2/HER2; Monoclonal Recombinant Antibody; Recombinant anti-human ErbB2/HER2 Antibody (Trastuzumab) FITC Conjugated; Receptor tyrosine-protein kinase erbB-2; Samceptin; Trastuzumab; ErbB2/HER2 recombinant antibody
Host
CHO cells
Reactivity
Human
Clonality
Monoclonal
Clone Number
ABM2A1G1
Purity/Purification
Purity: >98.0% as determined by SEC-HPLC & SDS-PAGE
Form/Format
Purified; Tris, 0.05% azide
Applicable Applications for ErbB2/HER2 recombinant antibody
Functional Assay (FA), Flow Cytometry (FC/FACS), Antibody-Dependent Cellular Cytotoxicity (ADCC)
Application Notes
FC/FACS: 1-2ug/10^6 cells
ADCC: 20-25mg/ml
ADCC: 20-25mg/ml
Antibody Type
Recombinant Antibody
Conjugation
FITC
Amino Acid Information
PepMap Analysis of N terminal amino acids sequence: H chain: EVQLVESGGGLVQPG, L chain: DIQMTQSPSSLSASV
Product Construction
Immunoglobulin G1, anti-(human p185neu receptor) (human-mouse monoclonal rhuMab HER2 g1- chain), disulfide with human-mouse monoclonal rhuMab HER2 light chain, dimer.
Preparation and Storage
Store the conjugated antibody at 4 degree C, stable for 12 months.
Shipping: Blue Ice pack
Shipping: Blue Ice pack
FCM (Flow Cytometry)
(Figure-6: Epitope binding study by flow cytometric analysis. MCF-7 cells expressing HER2 antigen were treated with Herceptin and Samceptin (2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
FCM (Flow Cytometry)
(Figure-6: Epitope binding study by flow cytometric analysis. MCF-7 cells expressing HER2 antigen were treated with Herceptin and Samceptin (2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
FCM (Flow Cytometry)
(Figure-5: Epitope binding study by flow cytometric analysis. BT-474 cells expressing HER2 antigen were treated with Herceptin and Samceptin (2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
FCM (Flow Cytometry)
(Figure-5: Epitope binding study by flow cytometric analysis. BT-474 cells expressing HER2 antigen were treated with Herceptin and Samceptin (2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
SDS-PAGE
(Figure-4: Reducing SDS-PGE of Samceptin (Lane-1) in comparison with Herceptin (Lane-2). Lanes were overloaded to show the presence of any other protein bands than Samceptin. Both heavy and light chains are well separated.)
SDS-PAGE
(Figure-4: Reducing SDS-PGE of Samceptin (Lane-1) in comparison with Herceptin (Lane-2). Lanes were overloaded to show the presence of any other protein bands than Samceptin. Both heavy and light chains are well separated.)
Activity
(Figure-3: SEC-HPLC analysis of Herceptin and Samceptin. Based on this analysis, Samceptin is highly pure and purity estimated to be >98%)
Activity
(Figure-3: SEC-HPLC analysis of Herceptin and Samceptin. Based on this analysis, Samceptin is highly pure and purity estimated to be >98%)
Activity
(Figure-2: Antiproliferative activity of Samceptin was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in MCF-7 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Activity
(Figure-2: Antiproliferative activity of Samceptin was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in MCF-7 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Activity
(Figure-1: Antiproliferative activity of Samceptin was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit in SK-BR-3 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Activity
(Figure-1: Antiproliferative activity of Samceptin was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit in SK-BR-3 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Related Product Information for ErbB2/HER2 recombinant antibody
The drug Trastuzumab is a monoclonal antibody and works by targeting breast cancer cells that overexpress the HER2 protein. Overexpression (or amplification) of the HER2 protein is associated with accelerated cell division. By binding to the protein receptors on these cells, Trastuzumab interrupts the growth signal, thereby slowing the growth and spread of tumors. Approximately 20% of breast cancers overexpress the HER2 protein. Amplification of HER2 in breast cancer cells has been correlated with adverse prognostic factors such as large tumor size, high nuclear grade, and decreased expression of estrogen and progesterone hormone receptors. HER2 amplification has also been associated with reduced disease-free survival, and overall survival, for women with node-positive or node negative disease. Trastuzumab (anti-p185, rhuMab HER2), which is a humanized monoclonal antibody that binds to the HER2 protein. HER-2 is a transmembrane spanning receptor-like protein, which is structurally related to the epidermal growth factor receptor and has been shown to inhibit the proliferation of human tumor cells that overexpress HER2 both in vitro and in vivo. The developed biosimilar to Herceptin, designated as Samceptin. The biochemical characterizations and the functional assays suggest that Samceptin is highly similar in structure and activity to Herceptin.
Product Categories/Family for ErbB2/HER2 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
137,910 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-2 isoform b
NCBI Official Synonym Full Names
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived on
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19
UniProt Entry Name
ERBB2_HUMAN
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Product Notes
The ErbB2/HER2 erbb2 (Catalog #AAA14887) is a Recombinant Antibody produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. The Recombinant anti-human ErbB2/HER2 Antibody (Trastuzumab) FITC Conjugated reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ErbB2/HER2 can be used in a range of immunoassay formats including, but not limited to, Functional Assay (FA), Flow Cytometry (FC/FACS), Antibody-Dependent Cellular Cytotoxicity (ADCC). FC/FACS: 1-2ug/10^6 cells ADCC: 20-25mg/ml. Researchers should empirically determine the suitability of the ErbB2/HER2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ErbB2/HER2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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