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WB (Western Blot)
(Detection of human HER2/ErbB2 by western blot. Samples: Whole cell lysate (10 ug) from LNCaP, HeLa, HT-29, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
HER2/ErbB2 recombinant antibody
Rabbit anti-HER2/ErbB2 Recombinant Monoclonal Antibody [BLR241L]
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
HER2/ErbB2, Recombinant Antibody; Rabbit anti-HER2/ErbB2 Recombinant Monoclonal Antibody [BLR241L]; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; NGL; p185erbB2; proto-oncogene c-ErbB-2; proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; herstatin; neuroblastoma/glioblastoma derived oncogene homolog; TKR1; HER2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; c-erb B2/neu protein; HER-2; HER-2/neu; human epidermal growth factor receptor 2; CD antigen CD340; MLN 19; NEU; CD340; Receptor tyrosine-protein kinase erbB-2; erb-b2 receptor tyrosine kinase 2; HER2/ErbB2 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR241L
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for HER2/ErbB2 recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1205 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HER2/ErbB2 by western blot. Samples: Whole cell lysate (10 ug) from LNCaP, HeLa, HT-29, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human HER2/ErbB2 by western blot. Samples: Whole cell lysate (10 ug) from LNCaP, HeLa, HT-29, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse HER2/ErbB2 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse HER2/ErbB2 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
ICC (Immunocytochemistry)
(Detection of human HER2/ErbB2 by immunocytochemistry. Sample: FFPE section of LNCaP cells. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657 lot 1) used for IP at 20 ul/mg lysate. HER2/ErbB2 was also immunoprecipitated by a second antibody against a different epitope of HER2/ErbB2 (A301-621A). For blotting immunoprecipitated HER2/ErbB2, AAA213657 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
ICC (Immunocytochemistry)
(Detection of human HER2/ErbB2 by immunocytochemistry. Sample: FFPE section of LNCaP cells. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657 lot 1) used for IP at 20 ul/mg lysate. HER2/ErbB2 was also immunoprecipitated by a second antibody against a different epitope of HER2/ErbB2 (A301-621A). For blotting immunoprecipitated HER2/ErbB2, AAA213657 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
FCM/FACS (Flow Cytometry)
(Detection of mouse HER2/ErbB2 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of mouse HER2/ErbB2 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human HER2/ErbB2 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human HER2/ErbB2 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody (AAA213657) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for HER2/ErbB2 recombinant antibody
ErbB2 is a member of the receptor tyrosine kinase family of proteins. The four members of this family are ErbB1 (Epidermal growth factor receptor; EGFR/HER1), ErbB2 (HER2/Neu), ErbB3 (HER3), and ErbB4 (HER4). The ErbB receptor members are single-pass transmembrane proteins with an extracellular ligand-binding domain and a cytoplasmic tyrosine-kinase domain. The epidermal growth factor (EGF) and its related proteins serve as the ligands for the ErbB receptors. Upon binding, the receptors heterodimerize, autophosphorylate and make contact with adapter molecules to transmit cellular signals involved in cell proliferation, survival, and differentiation. The ErbB2 receptor is the only member that does not interact with a ligand, but instead functions as a heterodimeric partner to coordinate signals from other ligand bound ErbB receptors. Amplification and/or overexpression of ErbB2 have been reported in many cancers.
Product Categories/Family for HER2/ErbB2 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,253 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-2 isoform a
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog;
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19
UniProt Entry Name
ERBB2_HUMAN
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Product Notes
The HER2/ErbB2 erbb2 (Catalog #AAA213657) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HER2/ErbB2 Recombinant Monoclonal Antibody [BLR241L] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HER2/ErbB2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HER2/ErbB2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HER2/ErbB2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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