WB (Western Blot)
(AAA330082 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(#AAA330082) and rabbit anti-beta tubulin Ab for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
Mouse HSP60 Monoclonal Antibody | anti-HSP60 antibody
HSP60 Mouse Monoclonal Antibody
Reactivity
Human, Mouse, RatPrediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Xenopus
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity-chromatography
Synonyms
HSP60, Antibody; HSP60 Mouse Monoclonal Antibody; 60 kDa chaperonin;60 kDa heat shock protein, mitochondrial;CH60_HUMAN;Chaperonin 60;Chaperonin, 60-KD;CPN60;fa04a05;GROEL;heat shock 60kDa protein 1 (chaperonin);Heat shock protein 1 (chaperonin);Heat shock protein 60;Heat shock protein 65;heat shock protein family D (Hsp60) member 1;HLD4;Hsp 60;HSP 65;HSP-60;HSP60;HSP65;HSPD1;HuCHA60;Mitochondrial matrix protein P1;P60 lymphocyte protein;short heat shock protein 60 Hsp60s1;SPG13; anti-HSP60 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Xenopus
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Xenopus
Clonality
Monoclonal
Isotype
Mouse IgG1
Specificity
HSP60 Antibody detects endogenous levels of total HSP60.
Purity/Purification
Affinity-chromatography
Form/Format
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence
MLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF
Applicable Applications for anti-HSP60 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human HSP60, corresponding to a region within C-terminal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(AAA330082 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(#AAA330082) and rabbit anti-beta tubulin Ab for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
WB (Western Blot)
(AAA330082 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(#AAA330082) and rabbit anti-beta tubulin Ab for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using HSP60 Mouse Monoclonal Antibody. Lane 1: RAW264.7 cells treated with blocking peptide; Lane 2: RAW264.7 cells, Lane 3: Hela cells, Lane 4: A549 cells, Lane 5: Rat brain tissue.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using HSP60 Mouse Monoclonal Antibody. Lane 1: RAW264.7 cells treated with blocking peptide; Lane 2: RAW264.7 cells, Lane 3: Hela cells, Lane 4: A549 cells, Lane 5: Rat brain tissue.)
Related Product Information for anti-HSP60 antibody
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Product Categories/Family for anti-HSP60 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HSP60 (Catalog #AAA330082) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSP60 Mouse Monoclonal Antibody reacts with Human, Mouse, Rat Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's HSP60 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the HSP60 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MLRLPTVFRQ MRPVSRVLAP HLTRAYAKDV KFGADARALM LQGVDLLADA VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKSIDLKDKY KNIGAKLVQD VANNTNEEAG DGTTTATVLA RSIAKEGFEK ISKGANPVEI RRGVMLAVDA VIAELKKQSK PVTTPEEIAQ VATISANGDK EIGNIISDAM KKVGRKGVIT VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TSKGQKCEFQ DAYVLLSEKK ISSIQSIVPA LEIANAHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL TLNLEDVQPH DLGKVGEVIV TKDDAMLLKG KGDKAQIEKR IQEIIEQLDV TTSEYEKEKL NERLAKLSDG VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVLGG GCALLRCIPA LDSLTPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKIMQSSSE VGYDAMAGDF VNMVEKGIID PTKVVRTALL DAAGVASLLT TAEVVVTEIP KEEKDPGMGA MGGMGGGMGG GMF. It is sometimes possible for the material contained within the vial of "HSP60, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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