Rabbit HSP60 (Heat Shock Protein 60) Monoclonal Antibody | anti-HSP60 antibody

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Cat. Num. AAA214390

• Gene Names: HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• Reactivity: Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey and Chicken.
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: HSP60 (Heat Shock Protein 60), Antibody; HSP60 (Heat Shock Protein 60) (Mitochondrial Marker); 60kDa chaperonin, 60kDa heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13); anti-HSP60 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey and Chicken.
• Clonality: Monoclonal
• Isotype: IgG, kappa
• Clone Number: HSPD1/2206R
• Specificity: Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone HSPD1/2206R, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
• Sequence Length: 573

• Applicable Applications for anti-HSP60 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human HSP60 protein
• Cellular Localization: Mitochondria in Cytoplasm
• Hu-chromosome Location: 2q33.1
• Positive Control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8°C.; Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified HSP60 Rabbit Recombinant Monoclonal Antibody (HSPD1/2206R). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot)

(Western blot analysis of HeLa cell lysate using HSP60 Rabbit Recombinant Monoclonal Antibody (HSPD1/2206R).)

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human liver stained with HSP60 Rabbit Recombinant Monoclonal Antibody (HSPD1/2206R).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded dog pancreas stained with HSP60 Rabbit Recombinant Monoclonal Antibody (HSPD1/2206R).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human liver stained with HSP60 Rabbit Recombinant Monoclonal Antibody (HSPD1/2206R).)

References

Boog CJ; de Graeff-Meeder ER; Lucassen MA; van der Zee R; Voorhorst-Ogink MM; van Kooten PJ; Geuze HJ; van Eden W. Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis. Journal of Experimental Medicine, 1992, 175(6):1805-10.

NCBI and Uniprot Product Information

• NCBI GI #: 31542947
• NCBI GeneID: 3329
• NCBI Accession #: NP_002147.2
• NCBI GenBank Nucleotide #: NM_002156.4
• UniProt Accession #: P10809; Q38L19; Q9UCR6; B2R5M6; B7Z712
• Molecular Weight: 60kDa
• NCBI Official Full Name: 60 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock protein family D (Hsp60) member 1
• NCBI Official Symbol: HSPD1
• NCBI Official Synonym Symbols: HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• UniProt Gene Name: HSPD1
• UniProt Synonym Gene Names: HSP60; CPN60; HSP-60; Hsp60