Mouse anti-Human, Mouse PCNA Monoclonal Antibody | anti-PCNA antibody

PCNA Antibody

Cat. Num. AAA290424

• Gene Names: PCNA; ATLD2
• Reactivity: Human, Mouse
• Applications: Immunoprecipitation, Immunocytochemistry, Western Blot
• Synonyms: PCNA, Antibody; PCNA Antibody; Purified Mouse Monoclonal Antibody (Mab); Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna/cyclin; PCNA_HUMAN; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen.; anti-PCNA antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Form/Format: ascites
• Sequence Length: 261

• Applicable Applications for anti-PCNA antibody: IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot)
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IP (Immunoprecipitation)

(Immunoprecipitation analysis of Hela cell lysates using PCNA mouse mAb.)

ICC (Immunocytochemistry)

(Immunocytochemistry staining of HeLa cells using PCNA mouse mAb (dilution 1:100).Fixed in 100% methanol for 2hr at -20 degree C.)

WB (Western Blot)

(Western blot detection of PCNA in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.)

Related Product Information for anti-PCNA antibody

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Product Categories/Family for anti-PCNA antibody

Cancer; Cell biology

References

Almendral J.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:1575-1579(1987). Travali S.,et al.J. Biol. Chem. 264:7466-7472(1989). Ota T.,et al.Nat. Genet. 36:40-45(2004). Deloukas P.,et al.Nature 414:865-871(2001). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 4505641
• NCBI GeneID: 5111
• NCBI Accession #: NP_002583.1
• NCBI GenBank Nucleotide #: NM_002592.2
• UniProt Accession #: P12004; B2R897; D3DW02
• Molecular Weight: 28769
• NCBI Official Full Name: proliferating cell nuclear antigen
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Official Synonym Symbols: ATLD2
• NCBI Protein Information: proliferating cell nuclear antigen
• UniProt Protein Name: Proliferating cell nuclear antigen
• UniProt Gene Name: PCNA
• UniProt Synonym Gene Names: PCNA
• UniProt Entry Name: PCNA_HUMAN