ICC (Immunocytochemistry)
(Anti-PCNA antibody (monoclonal), AAA44905, ICCICC: HELA Cell)
Mouse PCNA Monoclonal Antibody | anti-PCNA antibody
Anti-PCNA antibody (monoclonal)
Gene Names
PCNA; ATLD2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Ascites
Synonyms
PCNA, Antibody; Anti-PCNA antibody (monoclonal); Proliferating cell nuclear antigen; proliferating cell nuclear antigen; Cyclin; DNA POLYMERASE DELTA AUXILIARY PROTEIN; anti-PCNA antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
PC 10
Purity/Purification
Ascites
Form/Format
Lyophilized
Sequence Length
261
Applicable Applications for anti-PCNA antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Protein A fusion protein.
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(Anti-PCNA antibody (monoclonal), AAA44905, ICCICC: HELA Cell)
ICC (Immunocytochemistry)
(Anti-PCNA antibody (monoclonal), AAA44905, ICCICC: HELA Cell)
IHC (Immunohiostchemistry)
(Anti-PCNA antibody (monoclonal), AAA44905, IHC(P)IHC(P): Human Rectal Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-PCNA antibody (monoclonal), AAA44905, IHC(P)IHC(P): Human Rectal Cancer Tissue)
WB (Western Blot)
(Anti-PCNA antibody (monoclonal), AAA44905, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell LysateLane 3: HT1080 Cell LysateLane 4: COLO320 Cell Lysate)
WB (Western Blot)
(Anti-PCNA antibody (monoclonal), AAA44905, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell LysateLane 3: HT1080 Cell LysateLane 4: COLO320 Cell Lysate)
Related Product Information for anti-PCNA antibody
Description: Mouse IgG monoclonal antibody for PCNA, proliferating cell nuclear antigen (PCNA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, mouse, rat. No cross reactivity with other proteins. Uniprot ID: P12004
Background: Proliferating cell nuclear antigen(PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
Background: Proliferating cell nuclear antigen(PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Proliferating cell nuclear antigen
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
PCNA
NCBI Official Synonym Symbols
ATLD2
NCBI Protein Information
proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
UniProt Protein Name
Proliferating cell nuclear antigen
UniProt Gene Name
PCNA
UniProt Synonym Gene Names
PCNA
UniProt Entry Name
PCNA_HUMAN
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Product Notes
The PCNA pcna (Catalog #AAA44905) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PCNA antibody (monoclonal) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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