Mouse PCNA Monoclonal Antibody | anti-PCNA antibody

PCNA Mouse mAb

Cat. Num. AAA320371

• Reactivity: Human, Mouse, Monkey, Rat, Hamster
• Applications: Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Western Blot
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Synonyms: PCNA, Antibody; PCNA Mouse mAb; Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna/cyclin; PCNA_HUMAN; PCNAR; Polymerase delta accessory protein; Proliferating Cell Nuclear Antigen.; anti-PCNA antibody

• Host: Mouse
• Reactivity: Human, Mouse, Monkey, Rat, Hamster
• Clonality: Monoclonal
• Specificity: This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PCNA antibody: IHC (Immunohistochemistry), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Purified recombinant human PCNA protein fragments expressed in E coli.
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA mouse mAb (2E1-G10-H10.1:400 diluted).showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).)

ICC (Immunocytochemistry)

(Immunocytochemistry staining of HeLa cells using PCNA mouse mAb (dilution 1:100).Fixed in 100% methanol for 2hr at -20?.)

IP (Immunoprecipitation)

(Immunoprecipitation analysis of Hela cell lysates using PCNA mouse mAb.)

WB (Western Blot)

(Western blot detection of PCNA in Hela.3T3.COS7.C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.)

Related Product Information for anti-PCNA antibody

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Product Categories/Family for anti-PCNA antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4505641
• NCBI GeneID: 5111
• NCBI Accession #: NP_002583.1
• NCBI GenBank Nucleotide #: NM_002592.2
• UniProt Accession #: P12004; B2R897; D3DW02
• Molecular Weight: 28,769 Da
• NCBI Official Full Name: proliferating cell nuclear antigen
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Protein Information: proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
• UniProt Protein Name: Proliferating cell nuclear antigen
• UniProt Gene Name: PCNA
• UniProt Synonym Gene Names: PCNA
• UniProt Entry Name: PCNA_HUMAN