IF (Immunofluorescence)
(ICC/IF analysis of PPP1CA in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1CA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human PPP1CA Monoclonal Antibody | anti-PPP1CA antibody
PPP1CA antibody
Gene Names
PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
PPP1CA, Antibody; PPP1CA antibody; serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 1; protein phosphatase 1 catalytic subunit alpha isozyme; PP-1A; PP1alpha; ppp1a; MGC15877; MGC1674;; anti-PPP1CA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
4G3
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
330
Applicable Applications for anti-PPP1CA antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human PPP1CA (30-299aa) purified from E. coli
Clone Note
Anti-human PPP1CA mAb, clone 4G3, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPP1CA protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of PPP1CA in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1CA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of PPP1CA in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1CA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(Cell lysates of HepG2 (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(Cell lysates of HepG2 (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-PPP1CA antibody
Protein phosphatase 1, catalytic subunit, alpha isoform, also known as PPP1CA, is one of the three catalytic subunits of protein phosphatase 1. The protein is highly conserved in eukaryotic cells and play a role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. It is participate in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis and apoptosis
Product Categories/Family for anti-PPP1CA antibody
References
Cohen PT. (2002) J Cell Sci. 115 (2):241-56; Hsu LC, et al. (2006) Oncogene. 25 (40):5517-26
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,595 Da
NCBI Official Full Name
serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 1
NCBI Official Synonym Full Names
protein phosphatase 1 catalytic subunit alpha
NCBI Official Symbol
PPP1CA
NCBI Official Synonym Symbols
PP1A; PP-1A; PPP1A; PP1alpha
NCBI Protein Information
serine/threonine-protein phosphatase PP1-alpha catalytic subunit
UniProt Protein Name
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
UniProt Gene Name
PPP1CA
UniProt Synonym Gene Names
PPP1A; PP-1A
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Product Notes
The PPP1CA ppp1ca (Catalog #AAA48007) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PPP1CA antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1CA can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PPP1CA ppp1ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1CA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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