Application Data
(Immobilized Recombinant SARS-CoV-2 Spike RBD Protein (RP01278LQ) at 1ug/mL (100uL/well) can bind SARS-CoV-2 Spike RBD mAb (AAA283027) with a linear range of 0.78-50ng/mL.)
Mouse SARS-CoV-2 Spike RBD Monoclonal Antibody | anti-SpikeRBD antibody
SARS-CoV-2 Spike RBD Mouse mAb
Reactivity
SARS-COV-2
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Dot Blot, Western Blot
Purity
Affinity purification
Synonyms
SARS-CoV-2 Spike RBD, Antibody; SARS-CoV-2 Spike RBD Mouse mAb; spike glycoprotein; SARS-CoV-2 Spike RBD; anti-SpikeRBD antibody
Host
Mouse
Reactivity
SARS-COV-2
Clonality
Monoclonal
Isotype
IgG2b, kappa
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Applicable Applications for anti-SpikeRBD antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), DB (Dot Blot), WB (Western Blot)
Immunogen
Recombinant fusion protein of SARS-CoV-2 CoV spike RBD.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Application Data
(Immobilized Recombinant SARS-CoV-2 Spike RBD Protein (RP01278LQ) at 1ug/mL (100uL/well) can bind SARS-CoV-2 Spike RBD mAb (AAA283027) with a linear range of 0.78-50ng/mL.)
Application Data
(Immobilized Recombinant SARS-CoV-2 Spike RBD Protein (RP01278LQ) at 1ug/mL (100uL/well) can bind SARS-CoV-2 Spike RBD mAb (AAA283027) with a linear range of 0.78-50ng/mL.)
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SARS-CoV-2 Spike RBD using SARS-CoV-2 Spike RBD Mouse mAb (AAA283027) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SARS-CoV-2 Spike RBD using SARS-CoV-2 Spike RBD Mouse mAb (AAA283027) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Related Product Information for anti-SpikeRBD antibody
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and test antigen-based tests.
Product Categories/Family for anti-SpikeRBD antibody
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Product Notes
The SpikeRBD (Catalog #AAA283027) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 Spike RBD Mouse mAb reacts with SARS-COV-2 and may cross-react with other species as described in the data sheet. AAA Biotech's SARS-CoV-2 Spike RBD can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), DB (Dot Blot), WB (Western Blot). Researchers should empirically determine the suitability of the SpikeRBD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SARS-CoV-2 Spike RBD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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