FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling STIM1 with AAA62351 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue).Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Mouse anti-Human STIM1 Monoclonal Antibody | anti-STIM1 antibody
STIM1 Mouse mAb
Gene Names
STIM1; GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
STIM1, Antibody; STIM1 Mouse mAb; D11S4896E; GOK; stim1 {ECO:0000312|RGD:1306831}; Stromal interaction molecule 1; anti-STIM1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Sequence Length
685
Applicable Applications for anti-STIM1 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Source Note
This monoclonal antibody is produced by immunizing mice with a polypeptide corresponding to STIM1 protein.
Additional Information
Mass spectrum approved
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling STIM1 with AAA62351 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue).Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling STIM1 with AAA62351 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue).Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation)
(STIM1 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25218 of 10 ug.Western blot was performed from the immunoprecipitate using AAA62351 at 1/1000 dilution.Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution.Lane 1: THP1 cells membrane fraction.Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
IP (Immunoprecipitation)
(STIM1 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25218 of 10 ug.Western blot was performed from the immunoprecipitate using AAA62351 at 1/1000 dilution.Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution.Lane 1: THP1 cells membrane fraction.Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot)
(All lanes : STIM1 Mouse mAb at 1/2000 dilutionLane 1 : THP1 cells membrane fractionLane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 77 KDa Observed band size : 77 KDa Blocking/Dilution buffer : 1x TBST.)
WB (Western Blot)
(All lanes : STIM1 Mouse mAb at 1/2000 dilutionLane 1 : THP1 cells membrane fractionLane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 77 KDa Observed band size : 77 KDa Blocking/Dilution buffer : 1x TBST.)
Related Product Information for anti-STIM1 antibody
This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells.
Product Categories/Family for anti-STIM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted MW: 77kDa
NCBI Official Full Name
STIM1
NCBI Official Synonym Full Names
stromal interaction molecule 1
NCBI Official Symbol
STIM1
NCBI Official Synonym Symbols
GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
NCBI Protein Information
stromal interaction molecule 1
UniProt Protein Name
Stromal interaction molecule 1
UniProt Gene Name
STIM1
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Product Notes
The STIM1 stim1 (Catalog #AAA62351) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STIM1 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STIM1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the STIM1 stim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STIM1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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