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IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug STIM1 antibody (AAA283056 1:50). Western blot was performed from the immunoprecipitate using STIM1 antibody (A22488) at a dilition of 1:1000.)
Rabbit anti-Human STIM1 Monoclonal Antibody | anti-STIM1 antibody
STIM1 Rabbit PolymAb
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
STIM1, Antibody; STIM1 Rabbit PolymAb; GOK; TAM; TAM1; IMD10; STRMK; D11S4896E; anti-STIM1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
RQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK
Applicable Applications for anti-STIM1 antibody
ELISA, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Cross Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 530-685 of human STIM1. (NP_003147.2).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug STIM1 antibody (AAA283056 1:50). Western blot was performed from the immunoprecipitate using STIM1 antibody (A22488) at a dilition of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug STIM1 antibody (AAA283056 1:50). Western blot was performed from the immunoprecipitate using STIM1 antibody (A22488) at a dilition of 1:1000.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of paraffin-embedded Human liver tissue using STIM1 Rabbit PolymAb® (AAA283056) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis of paraffin-embedded Human liver tissue using STIM1 Rabbit PolymAb® (AAA283056) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using STIM1 Rabbit PolymAb® (AAA283056) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using STIM1 Rabbit PolymAb® (AAA283056) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot)
(Western blot analysis of various lysates, using STIM1 Rabbit PolymAb® (AAA283056) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot)
(Western blot analysis of various lysates, using STIM1 Rabbit PolymAb® (AAA283056) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
Related Product Information for anti-STIM1 antibody
This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.
Product Categories/Family for anti-STIM1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 77kDa
Observed MW: 77-85kDa
Observed MW: 77-85kDa
UniProt Protein Name
Stromal interaction molecule 1
UniProt Gene Name
STIM1
UniProt Synonym Gene Names
GOK
UniProt Entry Name
STIM1_HUMAN
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Product Notes
The STIM1 stim1 (Catalog #AAA283056) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STIM1 Rabbit PolymAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STIM1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the STIM1 stim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RQRVAPKPPQ MSRAADEALN AMTSNGSHRL IEGVHPGSLV EKLPDSPALA KKALLALNHG LDKAHSLMEL SPSAPPGGSP HLDSSRSHSP SSPDPDTPSP VGDSRALQAS RNTRIPHLAG KKAVAEEDNG SIGEETDSSP GRKKFPLKIF KKPLKK. It is sometimes possible for the material contained within the vial of "STIM1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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