Rabbit STIM1 Polyclonal Antibody | anti-STIM1 antibody

STIM1 Antibody

Cat. Num. AAA39585

• Gene Names: STIM1; GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: STIM1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: STIM1, Antibody; STIM1 Antibody; STIM1; GOK; TAM; IMD10; D11S4896E; Stromal interaction molecule 1; stromal interaction molecule 1; anti-STIM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
• Purity/Purification: STIM1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 685

• Applicable Applications for anti-STIM1 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1.
• Buffer: STIM1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: STIM1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of STIM1 in Hek293 cells with STIM1 antibody at 20 μg/mL.Red: STIM1 Antibody (4119)Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of STIM1 in mouse spleen tissue with STIM1 antibody at 20 μg/mL.Green: STIM1 Antibody (4119)Blue: DAPI staining)

IHC (Immunohistochemisry)

(Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5 μg/ml.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 μg/mL.)

Related Product Information for anti-STIM1 antibody

STIM1 Antibody: In T lymphocytes, the sole pathway for Ca²⁺ entry following antigen-receptor binding is through store-operated Ca²⁺-release-activated Ca²⁺ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca²⁺ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca²⁺ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca²⁺ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca²⁺ entry.

NCBI and Uniprot Product Information

• NCBI GI #: 21070997
• NCBI GeneID: 6786
• NCBI Accession #: NP_003147
• NCBI GenBank Nucleotide #: NM_003156.3
• UniProt Accession #: Q13586; Q8N382; E9PQJ4
• Molecular Weight: Predicted: 59; 75; 87 kDa; ; Observed: 88 kDa
• NCBI Official Full Name: stromal interaction molecule 1 isoform 2
• NCBI Official Synonym Full Names: stromal interaction molecule 1
• NCBI Official Symbol: STIM1
• NCBI Official Synonym Symbols: GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
• NCBI Protein Information: stromal interaction molecule 1
• UniProt Protein Name: Stromal interaction molecule 1
• UniProt Gene Name: STIM1
• UniProt Synonym Gene Names: GOK
• UniProt Entry Name: STIM1_HUMAN