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ELISA
(Figure 5: Binding activity of Recombinant Human PD-L1 Fc Fusion Protein to PD-1/CHO stable cell line (14-500ACL) was analyzed by cell based ELISA. PD-1/CHO cells were plated into 96 well ELISA plate over night. Next day, plate was washed in PBS and fixed in fixation buffer for 15 min. Cells were blocked in blocking reagent for 30 min. Then, Recombinant Human PD-L1 Fc Fusion Protein was added in different dilution to cells and incubated in room temp. for 1 hr. Plate was wash 3 times in TBST wash buffer. Goat anti-human HRP was added and incubated at room temp. for 30 min. Then plate was washed 4 times in TBST and analyzed in ELISA reader in 450 nm.)
PD-L1 Fc Fusion Recombinant Protein | PD-L1 recombinant protein
Recombinant Human PD-L1 Fc Fusion Protein (Active)
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA, Functional Assay
Purity
99% Purity SDS -PAGE and HPLC.
Synonyms
PD-L1 Fc Fusion; N/A; Recombinant Human PD-L1 Fc Fusion Protein (Active); B7 homolog 1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1 recombinant protein
Host
CHO-K1 celline
Purity/Purification
99% Purity SDS -PAGE and HPLC.
Form/Format
Lyophilized sterile PBS, 5% trehalose and 0.01% tween 80 are added as protectant before lyophilization.
Sequence
Human PD-L1 (ECD): MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER
Applicable Applications for PD-L1 recombinant protein
WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA, FA (Functional Assay)
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Preparation and Storage
Store it under sterile conditions at -4 degree C. It is recommended that the protein be aliquoted for optimal storage.
ELISA
(Figure 5: Binding activity of Recombinant Human PD-L1 Fc Fusion Protein to PD-1/CHO stable cell line (14-500ACL) was analyzed by cell based ELISA. PD-1/CHO cells were plated into 96 well ELISA plate over night. Next day, plate was washed in PBS and fixed in fixation buffer for 15 min. Cells were blocked in blocking reagent for 30 min. Then, Recombinant Human PD-L1 Fc Fusion Protein was added in different dilution to cells and incubated in room temp. for 1 hr. Plate was wash 3 times in TBST wash buffer. Goat anti-human HRP was added and incubated at room temp. for 30 min. Then plate was washed 4 times in TBST and analyzed in ELISA reader in 450 nm.)
ELISA
(Figure 5: Binding activity of Recombinant Human PD-L1 Fc Fusion Protein to PD-1/CHO stable cell line (14-500ACL) was analyzed by cell based ELISA. PD-1/CHO cells were plated into 96 well ELISA plate over night. Next day, plate was washed in PBS and fixed in fixation buffer for 15 min. Cells were blocked in blocking reagent for 30 min. Then, Recombinant Human PD-L1 Fc Fusion Protein was added in different dilution to cells and incubated in room temp. for 1 hr. Plate was wash 3 times in TBST wash buffer. Goat anti-human HRP was added and incubated at room temp. for 30 min. Then plate was washed 4 times in TBST and analyzed in ELISA reader in 450 nm.)
Application Data
(Figure-4: HPLC analysis of Recombinant Human PD-L1 Fc Fusion Protein)
Application Data
(Figure-4: HPLC analysis of Recombinant Human PD-L1 Fc Fusion Protein)
WB (Western Blot)
(Figure-2: Western blot analysis of Recombinant Human PDL-1 Fc Fusion Protein (0.5ug) using anti-human PD-L1 antibody .)
WB (Western Blot)
(Figure-2: Western blot analysis of Recombinant Human PDL-1 Fc Fusion Protein (0.5ug) using anti-human PD-L1 antibody .)
Application Data
(Figure-1: Recombinant Human PD-L1 Fc Fusion Protein. 0.5 ug protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.)
Application Data
(Figure-1: Recombinant Human PD-L1 Fc Fusion Protein. 0.5 ug protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.)
Related Product Information for PD-L1 recombinant protein
PD-L1 (CD274/B7-H1) is a critical membrane-bound costimulatory molecule belongs to the B7 superfamily that inhibits immune responses through its receptor, PD-1 and PD-L1 play a key role in the pathogenesis of inflammatory diseases (programmed death 1). It is widely expressed in the mononuclear phagocyte system (MPS), may co-stimulate T cells and regulates inflammatory responses. PD-L1 exerts inflammation regulatory functions via a negative co-stimulatory effect on T cell functions to inhibit cytokine secretion, facilitate apoptosis of activated T cells and induce T cell anergy. Aberrant expression and dysregulation of CD274 have been reported during bacterial infection, inflammation and in numerous autoimmune diseases.
Product Categories/Family for PD-L1 recombinant protein
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Product Notes
The PD-L1 (Catalog #AAA78593) is a Recombinant Protein produced from CHO-K1 celline and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PD-L1 Fc Fusion can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA, FA (Functional Assay). Researchers should empirically determine the suitability of the PD-L1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Human PD-L1 (ECD): MRIFAVFIFM TYWHLLNAFT VTVPKDLYVV EYGSNMTIEC KFPVEKQLDL AALIVYWEME DKNIIQFVHG EEDLKVQHSS YRQRARLLKD QLSLGNAALQ ITDVKLQDAG VYRCMISYGG ADYKRITVKV NAPYNKINQR ILVVDPVTSE HELTCQAEGY PKAEVIWTSS DHQVLSGKTT TTNSKREEKL FNVTSTLRIN TTTNEIFYCT FRRLDPEENH TAELVIPELP LAHPPNER. It is sometimes possible for the material contained within the vial of "PD-L1 Fc Fusion, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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