Podoplanin, soluble Recombinant Protein | Pdpn recombinant protein

Mouse Podoplanin, soluble

Cat. Num. AAA79189

• Gene Names: Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: Podoplanin, soluble; N/A; Mouse Podoplanin, soluble; Recombinant Mouse soluble Podoplanin; GP36; Glycoprotein 36; Aggrus; T1-alpha; Pdpn recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: GTIGVNEDDIVTPGT
• Sequence Length: 126

• Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
• Length(aa): 126
• Stabilizer: None
• Buffer: PBS
• Preparation and Storage: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 degree C. Reconstituted sPodoplanin should be stored in working aliquots at -20 degree C.

Application Data

(SDS-PAGE analysis of recombinant mouse soluble Podoplanin. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.)

Related Product Information for Pdpn recombinant protein

Podoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family. Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail. The ECD contains abundant Ser/Thr residues as potential sites for Oglycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation. Mouse Podoplanin shares 77% and 46% aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and on numerous tumors including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.

Product Categories/Family for Pdpn recombinant protein

Soluble receptors

References

1. Zimmer et al, Biochem J 341:277, 1999 2. KatsueInoue et al, J Biol Chem 282:25993, 2007 3. Kato et al, J Biol Chem 278:51599, 2003 4. Schacht et al, Am J Pathol 166:913, 2005 5. Breiteneder-Geleff et al, Am J Pathol 151:1141, 1997 6. Kato et al, Tumour Biol 26:195, 2005 7. Kato et al, Oncogene 23:8552, 2004 8. Mishima et al, Acta Neuropathol 111:563, 2006 9. Mishima et al, Acta Neuropathol 111:483, 2006 10. Kato et al, Biochem Biophys Res Commun 349:1301, 2006

NCBI and Uniprot Product Information

• NCBI GI #: 113462005
• NCBI GeneID: 14726
• NCBI Accession #: NP_034459.2
• NCBI GenBank Nucleotide #: NM_010329.2
• UniProt Accession #: Q62011; Q546R8; Q61612; A2A8J3
• Molecular Weight: 13,42 kDa
• NCBI Official Full Name: podoplanin
• NCBI Official Synonym Full Names: podoplanin
• NCBI Official Symbol: Pdpn
• NCBI Official Synonym Symbols: T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha
• NCBI Protein Information: podoplanin; aggrus; PA2.26 antigen; glycoprotein 38; transmembrane glycoprotein E11
• UniProt Protein Name: Podoplanin
• UniProt Gene Name: Pdpn
• UniProt Synonym Gene Names: Gp38; Ots8; Gp38; T1A
• UniProt Entry Name: PDPN_MOUSE