Polyclonal Antibodies

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ICC (Immunocytochemistry) (ICC staining MMP2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

MMP2, Polyclonal Antibody (Cat# AAA29966)

Full Name

MMP2 Antibody

Gene Names

MMP2; CLG4; MONA; CLG4A; TBE-1; MMP-II

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of 293T cells using anti-PTCD1 antibody (AAA19983).Overlay histogram showing 293T cells stained with AAA19983 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTCD1 Antibody (AAA19983, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of PTCD1 using anti-PTCD1 antibody (AAA19983).PTCD1 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SIHA whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat heart tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTCD1 antigen affinity purified polyclonal antibody (#AAA19983) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTCD1 at approximately 79 kDa. The expected band size for PTCD1 is at 79 kDa.)

PTCD1, Polyclonal Antibody (Cat# AAA19983)

Full Name

Anti-PTCD1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26895)

Full Name

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Gene Names

PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha

Reactivity

Human

Applications

Immunocytochemistry (ICC), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Purity

Purified by Immunoaffinity chromatography.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, IHC 1:50-300 IHC 1:50-300)

Tau, Polyclonal Antibody (Cat# AAA28943)

Full Name

Tau (phospho Ser396) Polyclonal Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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DNAJB1, Polyclonal Antibody (Cat# AAA29711)

Full Name

DNAJB1 Antibody

Gene Names

DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse brain using CAMK2G antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.)

CAMK2G, Polyclonal Antibody (Cat# AAA28433)

Full Name

CAMK2G Rabbit pAb

Gene Names

BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

GGT1, Polyclonal Antibody (Cat# AAA29201)

Full Name

GGT1 Polyclonal Antibody

Gene Names

GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

PPP1R3C, Polyclonal Antibody (Cat# AAA29713)

Full Name

PPP1R3C Antibody

Gene Names

PPP1R3C; PPP1R5

Reactivity

Human, Rat, Mouse

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Purified by antigen affinity column

Pricing

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HSPB8, Polyclonal Antibody (Cat# AAA10770)

Full Name

HSPB8 Polyclonal Antibody

Gene Names

HSPB8; H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western Blot; Samples: Lane1: Mouse Heart lysate; Lane2: Rat Heart lysate; Lane3: Rat Liver lysate;Primary Ab: 0.1ug/ml Rabbit Anti-Human PARK7 AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Parkinson Disease Protein 7 (PARK7), Polyclonal Antibody (Cat# AAA21266)

Full Name

Polyclonal Antibody to Parkinson Disease Protein 7 (PARK7)

Gene Names

PARK7; DJ1; DJ-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using PTPIP51/RMDN3 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

PTPIP51/RMDN3, Polyclonal Antibody (Cat# AAA22290)

Full Name

PTPIP51/RMDN3 Polyclonal Antibody

Gene Names

RMDN3; RMD3; RMD-3; FAM82C; FAM82A2; ptpip51

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-RAB1A Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Muscle)

RAB1A, Polyclonal Antibody (Cat# AAA23570)

Full Name

RAB1A antibody - middle region

Gene Names

RAB1A; RAB1; YPT1

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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Topoisomerase II alpha, Polyclonal Antibody (Cat# AAA29961)

Full Name

Topoisomerase II alpha Antibody

Gene Names

TOP2A; TOP2; TP2A

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-STRBP antibody (AAA19978).Overlay histogram showing MCF-7 cells stained with AAA19978 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STRBP Antibody (AAA19978, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of STRBP using anti-STRBP antibody (AAA19978).STRBP was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRBP Antibody (AAA19978) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRBP Antibody (AAA19978) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRBP Antibody (AAA19978) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRBP Antibody (AAA19978) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human PC-3 whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STRBP antigen affinity purified polyclonal antibody (#AAA19978) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (80 kDa. The expected band size for STRBP is at 74 kDa.)

STRBP, Polyclonal Antibody (Cat# AAA19978)

Full Name

Anti-STRBP Antibody Picoband

Gene Names

STRBP; p74; SPNR; ILF3L; HEL162

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysateSENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

SENP3, Polyclonal Antibody (Cat# AAA23562)

Full Name

SENP3 antibody - N-terminal region

Gene Names

SENP3; SSP3; Ulp1; SMT3IP1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

EOMES, Polyclonal Antibody (Cat# AAA26890)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by affinity chromatography.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.)

Stat1, Polyclonal Antibody (Cat# AAA28940)

Full Name

Stat1 (phospho Tyr701) Polyclonal Antibody

Gene Names

STAT1; CANDF7; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunoprecipitation (IP)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

PSG1, Polyclonal Antibody (Cat# AAA29196)

Full Name

PSG1 Polyclonal Antibody

Gene Names

PSG1; SP1; B1G1; PBG1; CD66f; PSBG1; PSG95; PSGGA; DHFRP2; PSBG-1; PSGIIA; FL-NCA-1/2; PS-beta-C/D; PS-beta-G-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Smad1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Smad1, Polyclonal Antibody (Cat# AAA22285)

Full Name

(KO Validated) Smad1 Polyclonal Antibody

Gene Names

SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

EOMES, Polyclonal Antibody (Cat# AAA26893)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by affinity chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using MSH6 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

MSH6, Polyclonal Antibody (Cat# AAA28426)

Full Name

MSH6 Rabbit pAb

Gene Names

CAPN1; CANP; muCL; CANP1; CANPL1; muCANP

Reactivity

Human, Mouse, Rat, MK

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Figure 9. IF analysis of E-Cadherin/Cdh1 using anti-E-Cadherin/Cdh1 antibody (AAA19723).E-Cadherin/Cdh1 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-E-Cadherin/Cdh1 Antibody (AAA19723) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of E-Cadherin/Cdh1 using anti-E-Cadherin/Cdh1 antibody (AAA19723).E-Cadherin/Cdh1 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-E-Cadherin/Cdh1 Antibody (AAA19723) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-E-Cadherin/Cdh1 Antibody (AAA19723) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-E-Cadherin/Cdh1 Antibody (AAA19723) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-E-Cadherin/Cdh1 Antibody (AAA19723) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E-Cadherin/Cdh1 antigen affinity purified polyclonal antibody (#AAA19723) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for E-Cadherin/Cdh1 at approximately 120-130 kDa. The expected band size for E-Cadherin/Cdh1 is at 97 kDa.)

E-cadherin/Cdh1, Polyclonal Antibody (Cat# AAA19723)

Full Name

Anti-E-cadherin/Cdh1 Antibody Picoband

Gene Names

Cdh1; Um; UVO; Ecad; E-cad; L-CAM; AA960649

Reactivity

Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using SUMO1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

SUMO1, Polyclonal Antibody (Cat# AAA22283)

Full Name

(KO Validated) SUMO1 Polyclonal Antibody

Gene Names

SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

EOMES, Polyclonal Antibody (Cat# AAA26891)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by affinity chromatography.

Pricing

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MAP1LC3B, Polyclonal Antibody (Cat# AAA28176)

Full Name

MAP1LC3B Polyclonal Antibody

Gene Names

MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with CD9 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

CD9, Polyclonal Antibody (Cat# AAA29968)

Full Name

CD9 Antibody

Gene Names

CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

Pricing

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Application Data (Detection of Mouse anti Human CD300a in a flow cytometric analysis of human peripheral blood monocytes using Rabbit F(ab')2 anti Mouse IgG:FITC (AAA12305))

IgG, Polyclonal Antibody (Cat# AAA12305)

Full Name

RABBIT F(ab')2 ANTI MOUSE IgG:FITC

Applications

Flow cytometry (FC/FACS)

Pricing

IHC (Immunohistchemistry) (Figure 9. IHC analysis of ERBB4 using anti-ERBB4 antibody (AAA19729).ERBB4 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat PC-12 whole cell lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB4 antigen affinity purified polyclonal antibody (#AAA19729) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ERBB4 at approximately 180 kDa. The expected band size for ERBB4 is at 147 kDa.)

ERBB4, Polyclonal Antibody (Cat# AAA19729)

Full Name

Anti-ERBB4 Antibody Picoband

Gene Names

ERBB4; HER4; p180erbB4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: K562 cell lysate; Lane4: MCF7 cell lysatePrimary Ab: 0.2ug/ml Rabbit Anti-Human PKM2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Pyruvate kinase isozymes M2 (PKM2), Polyclonal Antibody (Cat# AAA21265)

Full Name

Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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WB (Western Blot) (Human RPMI-8226WB Suggested Anti-KRAS antibody Titration: 1 ug/mLSample Type: Human RPMI-8226)

KRAS, Polyclonal Antibody (Cat# AAA23564)

Full Name

KRAS antibody - N-terminal region

Gene Names

KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2

Reactivity

Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Tested Species Reactivty: Human , Mouse

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

EOMES, Polyclonal Antibody (Cat# AAA26892)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by affinity chromatography.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with cMet antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

cMet, Polyclonal Antibody (Cat# AAA29965)

Full Name

cMet Antibody

Gene Names

Met; HGF; HGFR; Par4; c-Met; AI838057

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

Pricing

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WB (Western Blot) (WB Suggested Anti-PIM1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human heart)

PIM1, Polyclonal Antibody (Cat# AAA23566)

Full Name

PIM1 antibody - N-terminal region

Gene Names

PIM1; PIM

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-FAM111B antibody (AAA19994).Overlay histogram showing MCF-7 cells stained with AAA19994 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM111B Antibody (AAA19994, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of FAM111B using anti-FAM111B antibody (AAA19994).FAM111B was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hacat whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: human U2OS whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM111B antigen affinity purified polyclonal antibody (#AAA19994) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human Hela whole cell lysates,Lane 3: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM111B antigen affinity purified polyclonal antibody (#AAA19994) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FAM111B at approximately 85 kDa. The expected band size for FAM111B is at 85 kDa.)

FAM111B, Polyclonal Antibody (Cat# AAA19994)

Full Name

Anti-FAM111B Antibody Picoband

Gene Names

FAM111B; CANP; POIKTMP

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-IGF2BP1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate)

IGF2BP1, Polyclonal Antibody (Cat# AAA23578)

Full Name

IGF2BP1 antibody - N-terminal region

Gene Names

IGF2BP1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IL-28, Polyclonal Antibody (Cat# AAA29210)

Full Name

IL-28 Polyclonal Antibody

Gene Names

IFNL2; IL28A; IL-28A

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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FUT1, Polyclonal Antibody (Cat# AAA29722)

Full Name

FUT1 Antibody

Gene Names

FUT1; H; HH; HSC

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antigen affinity purification

Pricing

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IF (Immunofluorescence) (Figure 6 Immunofluorescence Validation of TMEM41B in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

TMEM41B, Polyclonal Antibody (Cat# AAA11035)

Full Name

TMEM41B (CT) Antibody

Reactivity

Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

TMEM41B Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistchemistry) (Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

TPRA1 / GPR175, Polyclonal Antibody (Cat# AAA12312)

Full Name

Rabbit Polyclonal to Human TPRA1 / GPR175

Gene Names

TPRA1; GPR175; TPRA40; TMEM227

Reactivity

Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Mouse, Pig, Rat
Predicted Reactivity: Monkey, Rabbit, Zebrafish (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin)

Purity

Immunoaffinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse kidney using ACE2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

ACE2, Polyclonal Antibody (Cat# AAA22296)

Full Name

ACE2 Polyclonal Antibody

Gene Names

ACE2; ACEH

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using RARS antibody. Blue: DAPI for nuclear staining.)

RARS, Polyclonal Antibody (Cat# AAA28184)

Full Name

RARS Polyclonal Antibody

Gene Names

RARS; HLD9; ArgRS; DALRD1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

VEGF-C, Polyclonal Antibody (Cat# AAA29208)

Full Name

VEGF-C Polyclonal Antibody

Gene Names

VEGFC; VRP; Flt4-L

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Western Blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1:500)

Cdk2/Cdc2, Polyclonal Antibody (Cat# AAA30744)

Full Name

Cdk2/Cdc2 (Phospho-Thr160) Rabbit Polyclonal Antibody

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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RCC1, Polyclonal Antibody (Cat# AAA10777)

Full Name

RCC1 Polyclonal Antibody

Gene Names

RCC1; CHC1; RCC1-I; SNHG3-RCC1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U20S cells using anti-GLI2 antibody (AAA19737).Overlay histogram showing U20S cells stained with AAA19737 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLI2 Antibody (AAA19737, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of GLI2 using anti-GLI2 antibody (AAA19737) and anti-Beta Tubulin antibody (M01857-3).GLI2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: rat brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (#AAA19737) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLI2 at approximately 180-200 kDa. The expected band size for GLI2 is at 220 kDa.)

GLI2, Polyclonal Antibody (Cat# AAA19737)

Full Name

Anti-GLI2 Antibody Picoband

Gene Names

GLI2; HPE9; PHS2; THP1; THP2

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HepG2 cells.)

CXXC1/CGBP, Polyclonal Antibody (Cat# AAA21273)

Full Name

Anti-CXXC1/CGBP Antibody

Gene Names

CXXC1; CFP1; CGBP; SPP1; PCCX1; PHF18; hCGBP; ZCGPC1; HsT2645; 2410002I16Rik; 5830420C16Rik

Reactivity

Human
Predicted: Mouse, Rat

Applications

Chromatin Immunoprecipitation sequencing (ChIP-Seq), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Affinity Purified