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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 50-100 of 3176 product results
Application Data
(Human peripheral blood lymphocytes were stained with Goat F(ab')2 Anti-Human IgM-PE and Mouse Anti-Human CD19-FITC .)
Goat F(ab')2 Anti-Human IgM (u chain specific), Polyclonal Secondary Antibody (Cat# AAA14901)
Full Name
Goat F(ab')2 Anti-Human IgM-FITC
Reactivity
Human
Applications
FC
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26872)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (AP)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
BRSV, Polyclonal Antibody (Cat# AAA14339)
Full Name
BRSV antibody
Applications
WB
Purity
BRSV antibody was purified by affinity chromatography.
Pricing
Influenza A, Polyclonal Antibody (Cat# AAA14340)
Full Name
Influenza A antibody
Applications
WB
Purity
Influenza A antibody was purified by Protein G affinity purification.
Pricing
unc-13 homolog A, Polyclonal Antibody (Cat# AAA15906)
Full Name
Rabbit anti-human unc-13 homolog A (C. elegans) polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Purity
Antigen Affinity Purified
Pricing
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 70 kDa isoform is identified, and a second isoform of 65 kDa is also present in some samples.)
SLC27A2, Polyclonal Antibody (Cat# AAA23511)
Full Name
SLC27A2 antibody - N-terminal region
Gene Names
SLC27A2; VLCS; FATP2; VLACS; ACSVL1; FACVL1; hFACVL1; HsT17226
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26877)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 405)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of PC3 cells using AAA26940 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
X-ray repair cross-complementing protein 6, Polyclonal Antibody (Cat# AAA26940)
Full Name
Rabbit anti-human X-ray repair cross-complementing protein 6 polyclonal Antibody
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human, mouse
Applications
EIA, WB, IHC, IF
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
Pseudomonas aeruginosa Beta-lactamase, Polyclonal Antibody (Cat# AAA18839)
Full Name
Rabbit anti-Pseudomonas aeruginosa Beta-lactamase polyclonal Antibody(ampC)
Reactivity
Pseudomonas aeruginosa
Applications
EIA
Purity
>95% Protein G purified
Pricing
SAA3, Polyclonal Antibody (Cat# AAA27938)
Full Name
SAA3 Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purified
Pricing
Eotaxin, Polyclonal Antibody (Cat# AAA14071)
Full Name
Rabbit anti Eotaxin Polyclonal Antibody
Reactivity
Human
Applications
EIA, IP, IHC, WB
Purity
Purified by Epitope Affinity Purification
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Human Kidney Tissue;Primary Ab: 30ug/ml Rabbit Anti-Human MIB2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Mindbomb Homolog 2 (MIB2), Polyclonal Antibody (Cat# AAA20152)
Full Name
Polyclonal Antibody to Mindbomb Homolog 2 (MIB2)
Gene Names
MIB2; ZZZ5; ZZANK1
Reactivity
Human
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26879)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 550)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26876)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (HRP)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H4, Polyclonal Antibody (Cat# AAA31346)
Full Name
Acetyl-Histone H4 (Lys8) Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat, Pig, Bovine
Predicted Reactivity: Chicken (100%), Xenopus (100%)
Predicted Reactivity: Chicken (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Lyn using anti-Lyn antibody (AAA19150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lyn antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lyn at approximately 59KD. The expected band size for Lyn is at 59KD.)
Lyn, Polyclonal Antibody (Cat# AAA19150)
Full Name
Anti-Lyn Picoband Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26873)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (APC)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Hepatitis B Virus Surface Antigen, Polyclonal Antibody (Cat# AAA27808)
Full Name
Anti-Hepatitis B Virus Surface Antigen Antibody
Reactivity
Hepatitis B virus
Applications
EIA
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
DUSP1/MKP1, Polyclonal Antibody (Cat# AAA31363)
Full Name
Phospho-DUSP1/MKP1 (Ser359) Antibody
Gene Names
DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Intrinsic Factor, Polyclonal Antibody (Cat# AAA14319)
Full Name
Intrinsic Factor antibody
Applications
User optimized
Pricing
Cationic trypsin, Polyclonal Antibody (Cat# AAA26987)
Full Name
Cationic trypsin Antibody
Reactivity
Dog
Applications
EIA
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Liver Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse REG3b AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Regenerating Islet Derived Protein 3 Beta, Polyclonal Antibody (Cat# AAA20952)
Full Name
Polyclonal Antibody to Regenerating Islet Derived Protein 3 Beta (REG3b)
Gene Names
Reg3b; HIP; Pap; PAP1; REG-III
Reactivity
Mouse, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26875)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (FITC)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat ovary using COL1A1 at dilution of 1:200 (40x lens).)
COL1A1, Polyclonal Antibody (Cat# AAA30654)
Full Name
COL1A1 Rabbit Polyclonal Antibody
Gene Names
COL1A1; OI4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
histone H4, Polyclonal Antibody (Cat# AAA31295)
Full Name
Acetyl-histone H4 (Lys16) Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
WB (Western Blot)
(Western Blot: 1: 293 cell extract control2: 293 cell expressing NA (H5N1))
NA (A/Vietnam/1203/2004)(H5N1, human), Polyclonal Antibody (Cat# AAA13721)
Full Name
Anti-NA (A/Vietnam/1203/2004)(H5N1, human)
Applications
WB, EIA
Purity
Immunoaffinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cells using TEAD1.)
TEAD1, Polyclonal Antibody (Cat# AAA30613)
Full Name
TEAD1 Rabbit Polyclonal Antibody
Gene Names
TEAD1; AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ChIP
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Histone H3, Polyclonal Antibody (Cat# AAA31345)
Full Name
Acetyl-Histone H3 (Lys14) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
FCM (Flow Cytometry)
(Figure 12. Flow Cytometry analysis of A431 cells using anti-DBC-1/CCAR2 antibody (AAA19486).Overlay histogram showing A431 cells stained with AAA19486 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBC-1/CCAR2 Antibody (AAA19486, 1 ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DBC-1/CCAR2, Polyclonal Antibody (Cat# AAA19486)
Full Name
Anti-DBC-1/CCAR2 Antibody Picoband
Gene Names
KIAA1967; DBC1; DBC-1; NET35; p30DBC; p30 DBC
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Dilution: IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000)
ETBR, Polyclonal Antibody (Cat# AAA29332)
Full Name
ETBR Polyclonal Antibody
Gene Names
EDNRB; ETB; ET-B; ETBR; ETRB; HSCR; WS4A; ABCDS; ET-BR; HSCR2
Reactivity
Human
Applications
WB, IHC-P
Pricing
WB (Western Blot)
(Western blot analysis of extracts of HCT116 cells, using Bcl-2 at 1:1000 dilution.HCT116 cells treated with different concentrations of IL-8.)
Bcl-2, Polyclonal Antibody (Cat# AAA30650)
Full Name
Bcl-2 Rabbit Polyclonal Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
HSL, Polyclonal Antibody (Cat# AAA31384)
Full Name
Phospho-HSL (Ser660) Antibody
Gene Names
LIPE; HSL; LHS
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (88%), Horse (89%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (88%), Horse (89%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)
FGFR2, Polyclonal Antibody (Cat# AAA28728)
Full Name
FGFR2 Antibody (N-term)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, mouse, rat, monkey
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Figure 9. IF analysis of PGP using anti-PGP antibody (AAA19740).PGP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-PGP Antibody (AAA19740) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
PGP, Polyclonal Antibody (Cat# AAA19740)
Full Name
Anti-PGP Antibody Picoband
Gene Names
PGP; PGPase
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)
Pyruvate Dehydrogenase Complex Component X, Polyclonal Antibody (Cat# AAA20946)
Full Name
Polyclonal Antibody to Pyruvate Dehydrogenase Complex Component X (PDHX)
Gene Names
PDHX; E3BP; OPDX; PDX1; proX; DLDBP; PDHXD
Reactivity
Human, Pig
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
Application Data
(FLISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-PE)
Goat Anti-Rat IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14908)
Full Name
Goat Anti-Rat IgG-HRP
Reactivity
Rat IgM; may react with immunoglobulins from other species.
Applications
ELISA
Purity
Affinity chromatography on pooled rat IgG covalently linked to agarose
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
AKT1, Polyclonal Antibody (Cat# AAA31364)
Full Name
Phospho-AKT1 (Ser124) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Bovine (82%), Horse (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (91%), Bovine (82%), Horse (100%), Dog (100%), Chicken (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30923 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Galectin 3, Polyclonal Antibody (Cat# AAA30923)
Full Name
Galectin 3 Antibody
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(AAA31089 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
AKT, Polyclonal Antibody (Cat# AAA31089)
Full Name
AKT Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western Blot analysis of 3T3 cells using Nanos Homologue 1 (NANOS1) Polyclonal Antibody diluted at 1:1500. Secondary antibody was diluted at 1:20000)
Nanos Homologue 1 (NANOS1), Polyclonal Antibody (Cat# AAA30770)
Full Name
Nanos Homologue 1 (NANOS1) Rabbit Polyclonal Antibody
Gene Names
NANOS1; NOS1; SPGF12
Reactivity
Human, Rat, Mouse
Applications
WB, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
RBL2, Polyclonal Antibody (Cat# AAA31451)
Full Name
Phospho-RBL2 (Thr642) Antibody
Gene Names
RBL2; Rb2; P130
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (89%), Sheep (100%), Rabbit (89%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (89%), Sheep (100%), Rabbit (89%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).)
RYK, Polyclonal Antibody (Cat# AAA28767)
Full Name
RYK Antibody
Gene Names
RYK; JTK5; RYK1; JTK5A; D3S3195
Reactivity
Human, mouse, rat
Applications
WB, EIA, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IP (Immunoprecipitation)
(Figure 11 Immunoprecipitation Validation in HEK293 cells (Kawai et al., 2004)HEK293 cells were transiently transfected with DYKDDDDK-IRF7. Ccell lysates were immunoprecipitated with control rabbit anti-mouse immunoglobulin serum (IgG) or anti-MyD88 (Ab1 and Ab2), followed by immunoblotting with anti-DYKDDDDK.)
MYD88, Polyclonal Antibody (Cat# AAA10944)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse, Rat
Applications
EIA, WB
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of SH-SY5Y cells using anti-Lumican/LUM antibody (AAA19744).Overlay histogram showing SH-SY5Y cells stained with AAA19744 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Lumican/LUM Antibody (AAA19744, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Lumican/LUM, Polyclonal Antibody (Cat# AAA19744)
Full Name
Anti-Lumican/LUM Antibody Picoband
Gene Names
LUM; LDC; SLRR2D
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in Recombinantprotein (80ng, 40ng, 20ng, 10ng)All lanes: Human IgG Fc antibody;HRPconjugated at 1:1000Predicted band size: 35 kDaObserved band size: 35 kDa)
IgG Fc fragment, Polyclonal Secondary Antibody (Cat# AAA15949)
Full Name
Rabbit anti-Human IgG Fc fragment polyclonal Antibody, HRP conjugated
Reactivity
Human
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat spleen using CORO1A Rabbit pAb.)
CORO1A, Polyclonal Antibody (Cat# AAA30712)
Full Name
CORO1A Rabbit Polyclonal Antibody
Gene Names
CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Tubulin alpha-1B/K-alpha-1, Polyclonal Antibody (Cat# AAA28396)
Full Name
Tubulin alpha-1B/K-alpha-1 Rabbit pAb
Gene Names
TUBA1B; K-ALPHA-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
Application Data
HA1 (A/chicken/HongKong/NT366/03)(H9N2), Polyclonal Antibody (Cat# AAA13717)
Full Name
Anti-HA1 (A/chicken/HongKong/NT366/03)(H9N2)
Applications
WB, EIA
Purity
Immunoaffinity chromatography
Pricing
WB (Western Blot)
(Western Blot analysis of HeLa cells using Acetyl-Ub (K27) Polyclonal Antibody. Secondary antibody was diluted at 1:20000)
Ub, Polyclonal Antibody (Cat# AAA30781)
Full Name
Ub (Acetyl-Lys27) Rabbit Polyclonal Antibody
Gene Names
UBA52; L40; CEP52; RPL40; HUBCEP52
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF, paraffin section, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IF (Immunofluorescence)
(Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 μg/ml.Red: Caspase-8 Antibody (3473)Blue: DAPI staining)
Caspase-8, Polyclonal Antibody (Cat# AAA10948)
Full Name
Caspase-8 Antibody
Gene Names
CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IF
Purity
Caspase-8 Antibody is affinity chromatography purified via peptide column.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.