Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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WB (Western Blot) (Western blot analysis of lysates from Hela, HepG2, HL-60 cell line (from left to right), using HIST1H2AG Antibody (Center). AAA28746 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody (Center). AAA28746 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody (Center). AAA28746 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody (Center). AAA28746 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody (Center). AAA28746 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells using HIST1H2AG Antibody (Center)(green) compared to an isotype control of rabbit IgG(blue). AAA28746 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

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HIST1H2AG, Polyclonal Antibody (Cat# AAA28746)

Full Name

HIST1H2AG Antibody (Center)

Gene Names

HIST1H2AI; H2A/c; H2AFC

Reactivity

Human (Predicted Reactivity: Rat, Mouse, Bovine, Xenopus, Yeast, Chicken, Monkey, Zebrafish, Drosophila)

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $475/2x0.08 mL $685/3x0.08 mL $885/4x0.08 mL $940/5x0.08 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-RAP1GAP antibody (AAA19793).Overlay histogram showing MCF-7 cells stained with AAA19793 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAP1GAP Antibody (AAA19793, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of RAP1GAP using anti-RAP1GAP antibody (AAA19793).RAP1GAP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAP1GAP Antibody (AAA19793) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAP1GAP Antibody (AAA19793) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAP1GAP Antibody (AAA19793) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAP1GAP Antibody (AAA19793) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates,Lane 3: human SH-SY5Y whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1GAP antigen affinity purified polyclonal antibody (#AAA19793) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAP1GAP at approximately 95 kDa. The expected band size for RAP1GAP is at 73 kDa.)

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RAP1GAP, Polyclonal Antibody (Cat# AAA19793)

Full Name

Anti-RAP1GAP Antibody Picoband

Gene Names

RAP1GAP; RAPGAP; RAP1GA1; RAP1GAP1; RAP1GAPII

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (CRHBP Antibody-Immunohistochemistry of paraffin-embedded rat heart, at a dilution of 1:100.)

IHC (Immunohistochemistry) (CRHBP Antibody-Immunohistochemistry of paraffin-embedded human prostate, at a dilution of 1:100.)

IHC (Immunohistochemistry) (CRHBP Antibody-Immunohistochemistry of paraffin-embedded mouse heart, at a dilution of 1:100.)

IHC (Immunohistochemistry) (CRHBP Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.)

WB (Western Blot) (CRHBP Antibody-Western blot analysis of extracts of various cells.)

ICC (Immunocytochemistry) (CRHBP Antibody-Immunofluorescence analysis of HeLa cells.)

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CRHBP, Polyclonal Antibody (Cat# AAA21329)

Full Name

CRHBP Rabbit anti-Human Polyclonal Antibody

Gene Names

CRHBP; CRFBP; CRF-BP

Reactivity

Mouse, Rat, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

Pricing

$650/0.05 mL $1,255/2x0.05 mL $1,825/3x0.05 mL $2,365/4x0.05 mL $2,680/5x0.05 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

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Cytokeratin 18, Polyclonal Antibody (Cat# AAA29009)

Full Name

Cytokeratin 18 Polyclonal Antibody

Gene Names

KRT18; K18; CYK18

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunoprecipitation (IP)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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LIR-6, Polyclonal Antibody (Cat# AAA29265)

Full Name

LIR-6 Polyclonal Antibody

Gene Names

LILRA1; LIR6; CD85I; LIR-6

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Anti-Kv2.1 Picoband antibody, AAA11600-7.JPGIHC(F): Mouse Brain Tissue)

IHC (Immunohistchemistry) (Anti-Kv2.1 Picoband antibody, AAA11600-6.JPGIHC(F): Rat Brain Tissue)

IHC (Immunohistochemistry) (Anti-Kv2.1 Picoband antibody, AAA11600-5.JPGIHC(P): Mouse Brain Tissue)

IHC (Immunohistochemistry) (Anti-Kv2.1 Picoband antibody, AAA11600-4.JPGIHC(P): Rat Brain Tissue)

IHC (Immunohistochemistry) (Anti-Kv2.1 Picoband antibody, AAA11600-3.JPGIHC(P): Human Lung Cancer Tissue)

Application Data (Anti-Kv2.1 Picoband antibody, AAA11600-2.jpgAll lanes: Anti KV2.1 (AAA11600) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugPredicted bind size: 96KDObserved bind size: 96KD)

Application Data (Anti-Kv2.1 Picoband antibody, AAA11600-1.jpgAll lanes: Anti KV2.1 (AAA11600) at 0.5ug/mlWB: Recombinant Human kv2.1 Protein 0.5ngPredicted bind size: 47KDObserved bind size: 47KD)

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Kv2.1, Polyclonal Antibody (Cat# AAA11600)

Full Name

Anti-Kv2.1 Antibody

Gene Names

KCNB1; DRK1; KV2.1; EIEE26; h-DRK1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-NKX2-8 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human heart)

WB (Western Blot) (Host: RabbitTarget Name: NKX2-8Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NKX2-8Sample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NKX2-8Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NKX2-8Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: MouseTarget Name: NKX2-8Sample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

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NKX2-8, Polyclonal Antibody (Cat# AAA23393)

Full Name

NKX2-8 antibody - C-terminal region

Gene Names

NKX2-8; NKX2H; NKX2.8; Nkx2-9

Reactivity

Cow, Dog, Human, Mouse, Pig, Rabbit, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.)

WB (Western Blot) (Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bid BH3 Domain Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-hBid-BH3 Pab in mouse lung tissue lysates (35ug/lane). hBid-BH3(arrow) was detected using the purified Pab.)

WB (Western Blot) (The anti-Bid BH3 domain Pab is used in Western blot to detect Bid BH3 in HL-60 cell lysate.)

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Bid, Polyclonal Antibody (Cat# AAA28769)

Full Name

Bid Antibody (BH3 Domain Specific)

Gene Names

BID; FP497

Reactivity

Human, mouse

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

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FN1, Polyclonal Antibody (Cat# AAA29025)

Full Name

FN1 Polyclonal Antibody

Gene Names

FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ZNF346 antibody at 1:1000 dilution.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using ZNF346 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using ZNF346 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ZNF346 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using ZNF346 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using ZNF346 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using ZNF346 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ZNF346 antibody at dilution of 1:100 .)

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ZNF346, Polyclonal Antibody (Cat# AAA30561)

Full Name

ZNF346 Polyclonal Antibody

Gene Names

ZNF346; JAZ; Zfp346

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using HIST1H2AG antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse liver using HIST1H2AG antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using HIST1H2AG antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using HIST1H2AG antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using HIST1H2AG antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using HIST1H2AG antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HIST1H2AG Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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HIST1H2AG, Polyclonal Antibody (Cat# AAA28259)

Full Name

HIST1H2AG Polyclonal Antibody

Gene Names

HIST1H2AG; H2AG; H2A/p; H2AFP; H2A.1b; pH2A/f

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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WB (Western Blot) (EREG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EREG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of EREG Antibody (C-term) in HepG2 cell line lysates (35ug/lane). EREG (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of lysates from A549, HepG2, U-87 MG cell line (from left to right), using EREG Antibody (C-term). AAA28771 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Anti-EREG Antibody (C-term)at 1:2000 dilution + U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-EREG Antibody (C-term) at 1:1000 dilutionLane 1: A549 whole cell lysatesLane 2: human placenta lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-EREG Antibody (C-term)at 1:2000 dilution + A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-EREG Antibody (C-term) at 1:2000 dilutionLane 1: HepG2 whole cell lysatesLane 2: A549 whole cell lysatesLane 3: NCI-H1299 whole cell lysatesLane 4: U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

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EREG, Polyclonal Antibody (Cat# AAA28771)

Full Name

EREG Antibody (C-term)

Gene Names

EREG; ER

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using MCM5 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using MCM5 antibody at dilution of 1:100 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MCM5 antibody at 1:1000 dilution.)

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MCM5, Polyclonal Antibody (Cat# AAA30563)

Full Name

MCM5 Polyclonal Antibody

Gene Names

MCM5; CDC46; P1-CDC46

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Host: RabbitTarget Name: GLI2Sample Type: JurkatLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 2.5ug/mLPeptide Concentration: 4.0ug/mLLysate Quantity: 25ug/laneGel Concentration: 6%-18%)

WB (Western Blot) (Host: RabbitTarget Name: GLI2Sample Type: HepG2 Whole Cell lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GLI2Sample Tissue: Human RPMI 8226 Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Sample Type :Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStopPrimary Antibody Dilution :1:50Secondary Antibody :Goat anti-rabbit-cy3Secondary Antibody Dilution :1:1500Color/Signal Descriptions :GLI2: Green Neural crest cells:: RedGene Name :GLI2Submitted by :Anonymous)

IHC (Immunohistochemistry) (Rabbit Anti-GLI2 AntibodyParaffin Embedded Tissue: Human IntestineCellular Data: Epithelial cells of intestinal glandAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-GLI2 AntibodyParaffin Embedded Tissue: Human HeartCellular Data: Myocardial cellsAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

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GLI2, Polyclonal Antibody (Cat# AAA23396)

Full Name

GLI2 antibody - middle region

Gene Names

GLI2; CJS; HPE9; PHS2; THP1; THP2

Reactivity

Human

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Protein A purified

Pricing

$445/0.1 mL $855/2x0.1 mL $1,245/3x0.1 mL $1,610/4x0.1 mL $1,800/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody at dilution of 1:100 (20x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using PLK4 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PLK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

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PLK4, Polyclonal Antibody (Cat# AAA28258)

Full Name

PLK4 Polyclonal Antibody

Gene Names

PLK4; SAK; STK18; MCCRP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

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FoxO1, Polyclonal Antibody (Cat# AAA29026)

Full Name

FoxO1 Polyclonal Antibody

Gene Names

FOXO1; FKH1; FKHR; FOXO1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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TERT, Polyclonal Antibody (Cat# AAA29282)

Full Name

TERT Polyclonal Antibody

Gene Names

TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Caco-2 cells using anti-EIF3B antibody (AAA19811).Overlay histogram showing Caco-2 cells stained with AAA19811 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF3B Antibody (AAA19811, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of EIF3B using anti-EIF3B antibody (AAA19811).EIF3B was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: human MCF-7 whole cell lysates,Lane 6: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3B antigen affinity purified polyclonal antibody (#AAA19811) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 115 kDa.)

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EIF3B, Polyclonal Antibody (Cat# AAA19811)

Full Name

Anti-EIF3B Antibody Picoband

Gene Names

EIF3B; PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-Mitofilin/IMMT antibody (AAA19806).Overlay histogram showing HepG2 cells stained with AAA19806 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mitofilin/IMMT Antibody (AAA19806, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of Mitofilin/IMMT using anti-Mitofilin/IMMT antibody (AAA19806).Mitofilin/IMMT was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: rat NRK whole cell lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse HEPA1/6 whole cell lysates,Lane 7: mouse MFC whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofilin/IMMT antigen affinity purified polyclonal antibody (#AAA19806) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Mitofilin/IMMT at approximately 80-90 kDa. The expected band size for Mitofilin/IMMT is at 84 kDa.)

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Mitofilin/IMMT, Polyclonal Antibody (Cat# AAA19806)

Full Name

Anti-Mitofilin/IMMT Antibody Picoband

Gene Names

IMMT; HMP; P87; P89; PIG4; PIG52; MINOS2; P87/89

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (CTNNB1/Beta Catenin Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CTNNB1/Beta Catenin Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CTNNB1/Beta Catenin Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CTNNB1/Beta Catenin Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CTNNB1/Beta Catenin Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (CTNNB1/Beta Catenin Antibody-Anti-CTNNB1 antibody (AAA21342, 20 ug/mL) yields a specific band on capillary Western analysis (Protein Simple, WES, 12-230 kDa separation module) in 0.25 mg/mL human beta-catenin overexpression lysate.)

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CTNNB1/Beta Catenin, Polyclonal Antibody (Cat# AAA21342)

Full Name

CTNNB1/Beta Catenin Rabbit anti-Human Polyclonal Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P), Simple Western Blot (SWB)

Purity

Immunoaffinity purified

Pricing

$650/0.05 mg $1,255/2x0.05 mg $1,825/3x0.05 mg $2,365/4x0.05 mg $2,680/5x0.05 mg

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WB (Western Blot) (WB Suggested Anti-SSBP2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:7812500Positive Control: Raji cell lysate)

IHC (Immunohistochemistry) (Rabbit Anti-SSBP2 AntibodyParaffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-SSBP2 AntibodyParaffin Embedded Tissue: Human BrainCellular Data: Nerve fibreAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-SSBP2 antibodyParaffin Embedded Tissue: Human Brain cell Cellular Data: Nerve fibre of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml.)

IHC (Immunohistochemistry) (Colon, myenteric plexus)

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SSBP2, Polyclonal Antibody (Cat# AAA23390)

Full Name

SSBP2 antibody - N-terminal region

Gene Names

SSBP2; HSPC116; SOSS-B2

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

WB (Western Blot) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

Application Data (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

Application Data (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

WB (Western Blot) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

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FAS, Polyclonal Antibody (Cat# AAA29022)

Full Name

FAS Polyclonal Antibody

Gene Names

FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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p53, Polyclonal Antibody (Cat# AAA29278)

Full Name

p53 Polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using GM13125 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using GM13125 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using GM13125 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using GM13125 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using GM13125 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Gm13125 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

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GM13125, Polyclonal Antibody (Cat# AAA28255)

Full Name

GM13125 Polyclonal Antibody

Gene Names

Pramef20; Gm13125; EG627009

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

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WB (Western Blot) (Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).)

WB (Western Blot) (The anti-RYK Pab is used in Western blot to detect RYK in Jurkat cell lysate.)

WB (Western Blot) (Western blot analysis of lysates from mouse lung and ovary tissue lysate(from left to right), using RYK Antibody (N175). AAA28767 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175). AAA28767 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

WB (Western Blot) (Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175). AAA28767 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

WB (Western Blot) (Western blot analysis of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N175). AAA28767 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RYK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

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RYK, Polyclonal Antibody (Cat# AAA28767)

Full Name

RYK Antibody

Gene Names

RYK; JTK5; RYK1; JTK5A; D3S3195

Reactivity

Human, mouse, rat

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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CD85c, Polyclonal Antibody (Cat# AAA29279)

Full Name

CD85c Polyclonal Antibody

Gene Names

LILRB5; LIR8; CD85C; LIR-8

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Mouse brain, using Phospho-Cytokeratin 18 (Ser51) Antibody. The lane on the left was treated with blocking peptide.)

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Cytokeratin 18, Polyclonal Antibody (Cat# AAA31327)

Full Name

Phospho-Cytokeratin 18 (Ser51) Antibody

Gene Names

KRT18; K18; CYK18

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with XRCC6 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with XRCC6 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). XRCC6 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of XRCC6 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

FCM (Flow Cytometry) (XRCC6 Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of XRCC6 (arrow) using rabbit polyclonal XRCC6 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XRCC6 gene.)

IHC (Immunohistochemistry) (XRCC6 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of XRCC6 Antibody (C-term) in A2058 cell line lysates (35ug/lane).XRCC6 (arrow) was detected using the purified Pab.)

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XRCC6, Polyclonal Antibody (Cat# AAA28768)

Full Name

XRCC6 Antibody (C-term)

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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TRAIL, Polyclonal Antibody (Cat# AAA29280)

Full Name

TRAIL Polyclonal Antibody

Gene Names

TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

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PTN, Polyclonal Antibody (Cat# AAA29284)

Full Name

PTN Polyclonal Antibody

Gene Names

PTN; HARP; HBNF; HBGF8; NEGF1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (AAA31076 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31076 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31076 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (BMP3 Antibody for IHC in human testis.)

WB (Western Blot) (Western blot analysis of extracts of various sample, using BMP3 antibody.)

WB (Western Blot) (Western blot analysis of BMP3 expression in HeLa cells, The lane on the left is treated with the antigen-specific peptide.)

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BMP3, Polyclonal Antibody (Cat# AAA31076)

Full Name

BMP3 Antibody

Gene Names

BMP3; BMP-3A

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

WB (Western Blot) ((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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Orexin receptor 1, Polyclonal Antibody (Cat# AAA13669)

Full Name

Goat anti-Orexin receptor 1 Antibody

Gene Names

HCRTR1; OX1R

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Sheep

Applications

Peptide ELISA (EIA), Western Blot (WB)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$455/0.1 mg $875/2x0.1 mg $1,270/3x0.1 mg $1,645/4x0.1 mg $1,815/5x0.1 mg

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-OTUB1 antibody (AAA19813).Overlay histogram showing MCF-7 cells stained with AAA19813 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OTUB1 Antibody (AAA19813, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of OTUB1 using anti-OTUB1 antibody (AAA19813).OTUB1 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OTUB1 Antibody (AAA19813) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OTUB1 Antibody (AAA19813) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OTUB1 Antibody (AAA19813) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OTUB1 Antibody (AAA19813) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUB1 antigen affinity purified polyclonal antibody (#AAA19813) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OTUB1 at approximately 36 kDa. The expected band size for OTUB1 is at 31 kDa.)

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OTUB1, Polyclonal Antibody (Cat# AAA19813)

Full Name

Anti-OTUB1 Antibody Picoband

Gene Names

OTUB1; OTB1; OTU1; HSPC263

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (KRT18/CK18/Cytokeratin 18 Antibody-Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.)

IHC (Immunohistochemistry) (KRT18/CK18/Cytokeratin 18 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (KRT18/CK18/Cytokeratin 18 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (KRT18/CK18/Cytokeratin 18 Antibody-Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (KRT18/CK18/Cytokeratin 18 Antibody-Western blot analysis of Cytokeratin 18 expression in HeLa (A); A431 (B); MCF7 (C) whole cell lysates.)

ICC (Immunocytochemistry) (KRT18/CK18/Cytokeratin 18 Antibody-Immunofluorescent analysis of Cytokeratin 18 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

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KRT18/CK18/Cytokeratin 18, Polyclonal Antibody (Cat# AAA21349)

Full Name

KRT18/CK18/Cytokeratin 18 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Gene Names

KRT18; K18; CYK18

Reactivity

Human

Applications

Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Immunoaffinity purified

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using GABARAP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human mammary cancer using GABARAP antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GABARAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

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GABARAP, Polyclonal Antibody (Cat# AAA28252)

Full Name

GABARAP Polyclonal Antibody

Gene Names

GABARAP; MM46; ATG8A; GABARAP-a

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using HSP90AA1 antibody at dilution of 1:200 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse testis using HSP90AA1 antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using HSP90AA1 antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using HSP90AA1 antibody at dilution of 1:200 .)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HSP90AA1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using HSP90AA1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody at 1:1000 dilution.)

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HSP90AA1, Polyclonal Antibody (Cat# AAA30556)

Full Name

HSP90AA1 Polyclonal Antibody

Gene Names

HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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AIRE, Polyclonal Antibody (Cat# AAA31324)

Full Name

Phospho-AIRE (Ser156) Antibody

Gene Names

AIRE; APS1; APSI; PGA1; AIRE1; APECED

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

Specificity Notes

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PGE2, Polyclonal Antibody (Cat# AAA14173)

Full Name

Anti-PGE2, Rabbit Polyclonal

Gene Names

PTGES2; GBF1; GBF-1; PGES2; C9orf15; mPGES-2

Applications

Radioimmunoassay (RIA), ELISA (EIA)

Purity

Not purified

Pricing

$380/1 mL $730/2x1 mL $1,060/3x1 mL $1,365/5x1 mL $1,370/4x1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SK-Br-3 cells using PTAR1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human breast carcinoma with PTAR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of PTAR1 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of PTAR1 Antibody (Center) in Jurkat cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of PTAR1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of PTAR1 Antibody (Center) in CEM cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.)

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PTAR1, Polyclonal Antibody (Cat# AAA28765)

Full Name

PTAR1 Antibody (Center)

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using SET antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human prostate using SET antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using SET antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using SET antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using SET antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using SET antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using SET antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using SET antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using SET antibody at dilution of 1:100 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SET antibody at 1:1000 dilution.)

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SET, Polyclonal Antibody (Cat# AAA30557)

Full Name

SET Polyclonal Antibody

Gene Names

SET; 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (PTGES2 Antibody-Immunohistochemistry of paraffin-embedded human kidney cancer tissue.)

IHC (Immunohistochemistry) (PTGES2 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (PTGES2 Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (PTGES2 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (PTGES2 Antibody-Western blot analysis of extracts of various cell lines.)

ICC (Immunocytochemistry) (PTGES2 Antibody-Immunofluorescence analysis of A549 cells.)

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PTGES2, Polyclonal Antibody (Cat# AAA21365)

Full Name

PTGES2 Rabbit anti-Human Polyclonal Antibody

Gene Names

PTGES2; GBF1; GBF-1; PGES2; C9orf15; mPGES-2

Reactivity

Mouse, Rat, Human

Applications

Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

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HSP27, Polyclonal Antibody (Cat# AAA29045)

Full Name

HSP27 Polyclonal Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using ERLIN1 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human placenta using ERLIN1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using ERLIN1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using ERLIN1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using ERLIN1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using ERLIN1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ERLIN1 antibody.)

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ERLIN1, Polyclonal Antibody (Cat# AAA29813)

Full Name

ERLIN1 Polyclonal Antibody

Gene Names

ERLIN1; KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (Caspase 3 Antibody for IHC in human colon tissue)

WB (Western Blot) (Western blot analysis of Caspase 3 expression in Etoposide treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA31093 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA31093 staining K-562 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IF (Immunofluorescence) (AAA31093 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts of various tissue sample, using Caspase 3 Antibody.)

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Caspase 3, Polyclonal Antibody (Cat# AAA31093)

Full Name

Caspase 3 Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

IHC (Immunohistochemistry) (IHC staining of immersion-fixed frozen section of mouse embryo (13 d.p.c.) using AAA14708 at 1.7ug/ml, with overnight incubation at 4°C. HRP-conjugated anti-goat secondary antibody and DAB staining were used for visualization of Reelin which in this sample was localized to the developing brain. The tissue section was counterstained with hematoxylin (blue).)

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Reelin, Polyclonal Antibody (Cat# AAA14708)

Full Name

Reelin

Gene Names

RELN; RL; LIS2; PRO1598

Reactivity

Mouse

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified
Purified by immunoaffinity chromatography

Pricing

$1,065/0.1 mg $2,065/2x0.1 mg $3,010/3x0.1 mg $3,900/4x0.1 mg $4,575/5x0.1 mg

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-GIPC1 antibody (AAA19828).Overlay histogram showing HepG2 cells stained with AAA19828 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GIPC1 Antibody (AAA19828, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of GIPC1 using anti-GIPC1 antibody (AAA19828).GIPC1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: rat skeletal muscle tissue lysates,Lane 4: rat brain tissue lysates,Lane 5: rat NRK whole cell lysates,Lane 6: mouse skeletal muscle tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse C2C12 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIPC1 antigen affinity purified polyclonal antibody (#AAA19828) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GIPC1 at approximately 40 kDa. The expected band size for GIPC1 is at 36 kDa.)

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GIPC1, Polyclonal Antibody (Cat# AAA19828)

Full Name

Anti-GIPC1 Antibody Picoband

Gene Names

GIPC1; NIP; GIPC; IIP-1; TIP-2; SEMCAP; C19orf3; Hs.6454; GLUT1CBP; RGS19IP1; SYNECTIN; SYNECTIIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-HEY1 AntibodyTitration: 2.5ug/mlPositive Control: Lung)

WB (Western Blot) (Host: RabbitTarget Name: HEY1Sample Type: Hela Whole Cell lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: HEY1Sample Tissue: Human 293TAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: HEY1Sample Tissue: Human 293T Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-HEY1 AntibodyParaffin Embedded Tissue: Human LungCellular Data: Alveolar cellsAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-HEY1 AntibodyParaffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

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HEY1, Polyclonal Antibody (Cat# AAA23412)

Full Name

HEY1 Antibody - C-terminal region

Gene Names

HEY1; CHF2; OAF1; HERP2; HESR1; HRT-1; NERP2; hHRT1; BHLHb31

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistchemistry) (AAA31092 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of STAT3 expression in Mouse liver lysate)

IF (Immunofluorescence) (AAA31092 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of STAT3 expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA31092 staining A-431 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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STAT3, Polyclonal Antibody (Cat# AAA31092)

Full Name

STAT3 Antibody

Gene Names

STAT3; APRF; HIES; ADMIO; ADMIO1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry) (At 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of H2B (acetyl K5) in lysates of HeLa cells(TSA 1M, 18 hr), using H2B (acetyl K5) Antibody(AF4355).)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells(TSA 1M, 18 hr), using Acetyl-H2B (Lys5) Antibody.Lane1 was treated with Ac-blocking peptide.Lane2 was treated with Non-Ac-blocking peptide.)

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H2B, Polyclonal Antibody (Cat# AAA31348)

Full Name

Acetyl-H2B (Lys5) Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Histone H4 (acetyl K8) in lysates of A431 cells(TSA 1M, 18 hr), using Histone H4 (acetyl K8) Antibody(AF4353).)

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Histone H4, Polyclonal Antibody (Cat# AAA31346)

Full Name

Acetyl-Histone H4 (Lys8) Antibody

Gene Names

HIST2H4B; H4/o

Reactivity

Human, Mouse, Rat, Pig, Bovine
Predicted Reactivity: Chicken (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.