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Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.


Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.


Viewing 250-300 of 10000 product results


product-image-AAA31147_WB8.jpg WB (Western Blot) (Western blot analysis of extracts of various sample,using Vimentin antibody.)

Vimentin, Polyclonal Antibody (Cat# AAA31147)

Full Name
Vimentin Antibody
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA30751_WB6.png WB (Western Blot) (Western Blot analysis of extracts from K562 cells, using AAT Polyclonal Antibody. Secondary antibody was diluted at 1:20000)

AAT, Polyclonal Antibody (Cat# AAA30751)

Full Name
AAT Rabbit Polyclonal Antibody
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA14726_IF6.jpg IF (Immunofluorescence) (Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using AAA14726 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Heat Shock Protein 27, Polyclonal Antibody (Cat# AAA14726)

Full Name
Heat Shock Protein 27, phosphorylated (Ser82) (HSP27)
Gene Names
Hsp27; 27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Pricing
product-image-AAA20006_FCM7.png FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U937 cells using anti-TMEM132B antibody (AAA20006).Overlay histogram showing U937 cells stained with AAA20006 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM132B Antibody (AAA20006, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

TMEM132B, Polyclonal Antibody (Cat# AAA20006)

Full Name
Anti-TMEM132B Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA28079_IHC6.jpg IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse cerebellum using RARA antibody at dilution of 1:100 (40x lens).)

RARA, Polyclonal Antibody (Cat# AAA28079)

Full Name
RARA Polyclonal Antibody
Gene Names
RARA; RAR; NR1B1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
product-image-AAA10917_IHC6.jpg IHC (Immunohistchemistry) (Validation of PKR in Rat Lung Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (AAA10917) at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

PKR, Polyclonal Antibody (Cat# AAA10917)

Full Name
PKR Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PKR Antibody is affinity chromatography purified via peptide column.
Pricing
product-image-AAA19736_FCM9.png FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-MLKL antibody (AAA19736).Overlay histogram showing MCF-7 cells stained with AAA19736 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MLKL Antibody (AAA19736, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MLKL, Polyclonal Antibody (Cat# AAA19736)

Full Name
Anti-MLKL Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA10974_IF6.gif IF (Immunofluorescence) (Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 μg/ml.Red: MAGEA4 Antibody (8191)Blue: DAPI staining)

MAGEA4, Polyclonal Antibody (Cat# AAA10974)

Full Name
MAGEA4 Antibody
Gene Names
MAGEA4; CT1.4; MAGE4; MAGE4A; MAGE4B; MAGE-41; MAGE-X2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
MAGEA4 antibody is affinity chromatography purified via peptide column.
Pricing
product-image-AAA29881_FCM9.jpg FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with HDAC2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

Histone Deacetylase 2, Polyclonal Antibody (Cat# AAA29881)

Full Name
Histone Deacetylase 2 Antibody
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Protein affinity purified
Pricing
product-image-AAA31057_IHC6.jpg IHC (Immunohistchemistry) (AAA31057 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Acetyl-Histone H3, Polyclonal Antibody (Cat# AAA31057)

Full Name
Acetyl-Histone H3 (Lys9) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity purified
Pricing
product-image-AAA13880_WB3.jpg WB (Western Blot) (Anti-tdTomato Ab conjugated to DyLight 550 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane;)

tdTomato, Polyclonal Antibody (Cat# AAA13880)

Full Name
Anti-tdTomato, DyLight550
Reactivity
Reacts with Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing
product-image-AAA19746_FCM9.png FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-APLP2 antibody (AAA19746).Overlay histogram showing HepG2 cells stained with AAA19746 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APLP2 Antibody (AAA19746, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

APLP2, Polyclonal Antibody (Cat# AAA19746)

Full Name
Anti-APLP2 Antibody Picoband
Gene Names
APLP2; APPH; APPL2; CDEBP; APLP-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA21029_IHC7.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)

Protein Kinase C Iota, Polyclonal Antibody (Cat# AAA21029)

Full Name
Protein Kinase C Iota (PKCi) Polyclonal Antibody
Gene Names
PRKCI; PKCI; DXS1179E; nPKC-iota
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Pricing
product-image-AAA28843_IHC6.jpg IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.)

Histone H3, Polyclonal Antibody (Cat# AAA28843)

Full Name
Histone H3 (Di Methyl Lys10) Polyclonal Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
product-image-AAA29149_IHC7.jpg IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Prothrombin, Polyclonal Antibody (Cat# AAA29149)

Full Name
Prothrombin Polyclonal Antibody
Gene Names
F2; PT; THPH1; RPRGL2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
product-image-AAA31341_APP6.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Twist2, Polyclonal Antibody (Cat# AAA31341)

Full Name
Twist2 Antibody
Gene Names
TWIST2; AMS; FFDD3; BBRSAY; DERMO1; SETLSS; bHLHa39
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (92%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
product-image-AAA26894_WB6.jpg WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26894)

Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
product-image-AAA11043_IHC7.jpg IHC (Immunohistochemistry) (Figure 7 Immunohistochemistry Validation of IFI16 in Rat TestisImmunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody (AAA11043) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IFI16, Polyclonal Antibody (Cat# AAA11043)

Full Name
IFI16 (CT) Antibody
Gene Names
IFI16; PYHIN2; IFNGIP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
IFI16 Antibody is affinity chromatography purified via peptide column.
Pricing
product-image-AAA14101_WB.jpg WB (Western Blot) (Western blot analysis of A431cells treated with calyculin A (10 nM) for 30 min. The blot was probed with four different rabbit polyclonal antibodies (lane 1: anti-Akt (Thr-34), lane 2: anti-β-Catenin, lane 3: anti-N-WASP (Ser-484/Ser-485), lane 4: anti-Paxillin (Ser-178). This panel of primary antibodies was then detected using goat anti-rabbit Ig:HRP (1:5,000).)

Ig, Polyclonal Secondary Antibody (Cat# AAA14101)

Full Name
Anti-Rabbit Ig:HRP Secondary Antibody
Reactivity
Rabbit
Applications
Western Blot
Pricing
product-image-AAA21331_IHC7.jpg IHC (Immunohistochemistry) (PSMB8/LMP7 Antibody-Immunohistochemistry of paraffin-embedded rat heart, at a dilution of 1:100.)

PSMB8/LMP7, Polyclonal Antibody (Cat# AAA21331)

Full Name
PSMB8/LMP7 Rabbit anti-Human Polyclonal Antibody
Gene Names
PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
Reactivity
Mouse, Rat, Human
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
product-image-AAA28312_IF7.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K64 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

TriMethyl-Histone H3-K64, Polyclonal Antibody (Cat# AAA28312)

Full Name
TriMethyl-Histone H3-K64 Rabbit pAb
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
product-image-AAA10949_IHC6.gif IHC (Immunohistchemistry) (Immunohistochemistry of MFSD2A in human brain tissue with MFSD2A antibody at 5 μg/mL.)

MFSD2A, Polyclonal Antibody (Cat# AAA10949)

Full Name
MFSD2A Antibody
Gene Names
mfsd2aa; NLS1-A; mfsd2a; zgc:101615
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
MFSD2A Antibody is affinity chromatography purified via peptide column.
Pricing
product-image-AAA19465_FCM7.jpg FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SiHa cells using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (AAA19465).Overlay histogram showing SiHa cells stained with AAA19465 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GPCR RDC1/CXCR-7/ACKR3 Antibody (AAA19465, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

GPCR RDC1/CXCR-7/ACKR3, Polyclonal Antibody (Cat# AAA19465)

Full Name
Anti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband
Gene Names
CXCR7; RDC1; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA21266_WB6.jpg WB (Western Blot) (Western Blot; Samples: Lane1: Mouse Heart lysate; Lane2: Rat Heart lysate; Lane3: Rat Liver lysate;Primary Ab: 0.1ug/ml Rabbit Anti-Human PARK7 AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Parkinson Disease Protein 7 (PARK7), Polyclonal Antibody (Cat# AAA21266)

Full Name
Polyclonal Antibody to Parkinson Disease Protein 7 (PARK7)
Gene Names
PARK7; DJ1; DJ-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
product-image-AAA28724_IHC7.jpg IHC (Immunohistochemistry) (ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

ATP5B, Polyclonal Antibody (Cat# AAA28724)

Full Name
ATP5B Antibody (Center)
Gene Names
ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA29057_IHC7.jpg IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Ki-67, Polyclonal Antibody (Cat# AAA29057)

Full Name
Ki-67 Polyclonal Antibody
Gene Names
MKI67; KIA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
product-image-AAA30746_APP9.png Application Data (Li, X., Ma, N., Zhang, Y. et al. Circular RNA circNRIP1 promotes migration and invasion in cervical cancer by sponging miR-629-3p and regulating the PTP4A1/ERK1/2 pathway. Cell Death Dis 11, 399 (2020).)

ERK 1/2, Polyclonal Antibody (Cat# AAA30746)

Full Name
ERK 1/2 (Phospho-Thr202/Y204) Rabbit Polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA31085_IHC20.jpg IHC (Immunohistochemistry) (AAA31085 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

C-Kit, Polyclonal Antibody (Cat# AAA31085)

Full Name
C-Kit Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117; MASTC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing

Pseudomonas aeruginosa Beta-lactamase, Polyclonal Antibody (Cat# AAA18839)

Full Name
Rabbit anti-Pseudomonas aeruginosa Beta-lactamase polyclonal Antibody(ampC)
Reactivity
Pseudomonas aeruginosa
Applications
EIA
Purity
>95% Protein G purified
Pricing
product-image-AAA27061_WB.png WB (Western Blot) (Positive WB detected in: recombinant proteinAll lanes: gH Antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 kDaObserved band size: 38 kDa)

gH, Polyclonal Antibody (Cat# AAA27061)

Full Name
Rabbit anti-Epstein-Barr virus (strain B95-8)(HHV-4)(Human herpesvirus 4) gH Polyclonal Antibody
Reactivity
Epstein-Barr virus
Applications
Western Blot
Purity
>95%, Protein G purified
Pricing
product-image-AAA31443_APP6.jpg Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Caspase 3, Polyclonal Antibody (Cat# AAA31443)

Full Name
Phospho-Caspase 3 (Ser26) Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Rabbit (88%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA21281_WB6.jpg WB (Western Blot) (Western blot analysis of THP1 cell and mouse spleen cell lysate.)

NCF2/NOXA2/p67phox, Polyclonal Antibody (Cat# AAA21281)

Full Name
Anti-NCF2/NOXA2/p67phox Antibody
Gene Names
NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
Reactivity
Human
Predicted: Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Affinity Purified
Pricing
product-image-AAA29966_ICC6.jpg ICC (Immunocytochemistry) (ICC staining MMP2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

MMP2, Polyclonal Antibody (Cat# AAA29966)

Full Name
MMP2 Antibody
Gene Names
MMP2; CLG4; MONA; CLG4A; TBE-1; MMP-II
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
product-image-AAA31307_APP6.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

EPRS, Polyclonal Antibody (Cat# AAA31307)

Full Name
Phospho-EPRS (Ser886) Antibody
Gene Names
EPRS; EARS; PARS; QARS; QPRS; PIG32; GLUPRORS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA31410_APP6.jpg Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

PKC theta, Polyclonal Antibody (Cat# AAA31410)

Full Name
Phospho-PKC theta (Tyr90) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA19992_FCM7.jpg FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SH-SY5Y cells using anti-PLD5 antibody (AAA19992).Overlay histogram showing SH-SY5Y cells stained with AAA19992 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLD5 Antibody (AAA19992, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

PLD5, Polyclonal Antibody (Cat# AAA19992)

Full Name
Anti-PLD5 Antibody Picoband
Gene Names
PLD5; PLDC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA10960_WB8.gif WB (Western Blot) (Western blot analysis of SQSTM1 expression in K562 cell lysate with AP3B2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

SQSTM1, Polyclonal Antibody (Cat# AAA10960)

Full Name
SQSTM1 Antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SQSTM1 Antibody is affinity chromatography purified via peptide column.
Pricing
product-image-AAA29150_IHC6.jpg IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

L-Selectin, Polyclonal Antibody (Cat# AAA29150)

Full Name
L-Selectin Polyclonal Antibody
Gene Names
SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
product-image-AAA31279_IHC11.jpg IHC (Immunohistochemistry) (At 1/100 staining Human prostate cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Calsenilin/KCNIP3, Polyclonal Antibody (Cat# AAA31279)

Full Name
Phospho-Calsenilin/KCNIP3 (Ser63) Antibody
Gene Names
KCNIP3; CSEN; DREAM; KCHIP3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA20152_IHC6.png IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: Human Kidney Tissue;Primary Ab: 30ug/ml Rabbit Anti-Human MIB2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Mindbomb Homolog 2 (MIB2), Polyclonal Antibody (Cat# AAA20152)

Full Name
Polyclonal Antibody to Mindbomb Homolog 2 (MIB2)
Gene Names
MIB2; ZZZ5; ZZANK1
Reactivity
Human
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
product-image-AAA21279_WB6.jpg WB (Western Blot) (Western blot analysis of extracts of various cells.)

POLK/DNA Polymerase Kappa, Polyclonal Antibody (Cat# AAA21279)

Full Name
Anti-POLK/DNA Polymerase Kappa Antibody
Gene Names
POLK; DINP; POLQ; DINB1
Reactivity
Human
Predicted: Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
product-image-AAA29877_FCM6.jpg FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with SIRT3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)

SIRT3, Polyclonal Antibody (Cat# AAA29877)

Full Name
SIRT3 Antibody
Gene Names
SIRT3; SIR2L3
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
product-image-AAA31452_APP8.jpg Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

FGFR2, Polyclonal Antibody (Cat# AAA31452)

Full Name
Phospho-FGFR2 (Ser782) Antibody
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (88%), Bovine (100%), Horse (100%), Rabbit (83%), Dog (100%), Chicken (100%), Xenopus (88%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
product-image-AAA28191_IP7.jpg IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug SSBP1 antibody. Western blot was performed from the immunoprecipitate using SSBP1 antibody at a dilition of 1:1000.)

SSBP1, Polyclonal Antibody (Cat# AAA28191)

Full Name
SSBP1 Polyclonal Antibody
Gene Names
SSBP1; SSBP; mtSSB; Mt-SSB; SOSS-B1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
product-image-AAA26879_WB7.jpg WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26879)

Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 550)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
product-image-AAA11598_IHC7.jpg IHC (Immunohistochemistry) (Anti-CD3 epsilon Picoband antibody, pb9093-7.JPGIHC(F): Mouse Spleen Tissue)

CD3 epsilon, Polyclonal Antibody (Cat# AAA11598)

Full Name
Anti-CD3 epsilon Antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA21311_IHC6.jpg IHC (Immunohistchemistry) (TCP1 Antibody-Immunohistochemistry of paraffin-embedded mouse testis using TCP1 antibody at dilution of 1:100 (200x lens).)

TCP1, Polyclonal Antibody (Cat# AAA21311)

Full Name
TCP1 Rabbit anti-Human Polyclonal Antibody
Gene Names
TCP1; CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha
Reactivity
Mouse, Rat, Human
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
product-image-AAA10934_IF10.gif IF (Immunofluorescence) (Figure 10 Immunofluorescence images of PUMA in the P14 rat retina (Wakabayashi et al., 2012)PUMA expression in the rat retina detected by anti-PUMA antibodies (3043). The specimens were counterstained with Hoechst 33258 to visualize nuclei (+DNA). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; P, postnatal day.)

PUMA, Polyclonal Antibody (Cat# AAA10934)

Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
product-image-AAA21265_WB6.jpg WB (Western Blot) (Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: K562 cell lysate; Lane4: MCF7 cell lysatePrimary Ab: 0.2ug/ml Rabbit Anti-Human PKM2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Pyruvate kinase isozymes M2 (PKM2), Polyclonal Antibody (Cat# AAA21265)

Full Name
Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
product-image-AAA10676_IHC6.jpg IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Human kidney using PRKCE antibody at dilution of 1:100 (40x lens).)

PRKCE, Polyclonal Antibody (Cat# AAA10676)

Full Name
PRKCE Polyclonal Antibody
Gene Names
PRKCE; PKCE; nPKC-epsilon
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Purification
Pricing

What are Polyclonal Antibodies?


Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).


Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.


Key Uses of Polyclonal Antibodies


  • Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

  • Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

  • ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

  • Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

  • Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.


Why Buy Polyclonal Antibodies from AAA Biotech?


1. Ideal for Various Applications


Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.


2. Rigorous Quality Control


All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.


3. Wide Assortment of Antibodies


Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.


4. Highly Purified


Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.


FAQ


1. How are polyclonal antibodies produced?


Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.


2. How do polyclonal antibodies differ from monoclonal antibodies?


Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.


3. How should I store polyclonal antibodies?


Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.


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