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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 200-250 of 3176 product results
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of RAW264.7 cells using anti-APP-1/PABPC4 antibody (AAA19592).Overlay histogram showing RAW264.7 cells stained with AAA19592 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APP-1/PABPC4 Antibody (AAA19592, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
APP-1/PABPC4, Polyclonal Antibody (Cat# AAA19592)
Full Name
Anti-APP-1/PABPC4 Antibody Picoband
Gene Names
PABPC4; APP1; APP-1; PABP4; iPABP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
DRP1, Polyclonal Antibody (Cat# AAA30750)
Full Name
DRP1 Rabbit Polyclonal Antibody
Gene Names
DNM1L; DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV
Reactivity
Human, Mouse, Rat
Applications
IF, ICC, WB, IHC-P, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H3, Polyclonal Antibody (Cat# AAA31356)
Full Name
Acetyl-Histone H3 (Lys9/14/18/23/27) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Smad2, Polyclonal Antibody (Cat# AAA31390)
Full Name
Phospho-Smad2 (Ser255) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
Vimentin, Polyclonal Antibody (Cat# AAA28432)
Full Name
Vimentin Rabbit pAb
Gene Names
ABCA12; LI2; ICR2B; ARCI4A; ARCI4B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IRF7, Polyclonal Antibody (Cat# AAA31458)
Full Name
Phospho-IRF7 (Ser477) Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HEPG2 cells using anti-Alkaline phosphatase/ALPL antibody (AAA19231).Overlay histogram showing HEPG2 cells stained with AAA19231 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alkaline phosphatase/ALPL Antibody (AAA19231, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Alkaline phosphatase/ALPL, Polyclonal Antibody (Cat# AAA19231)
Full Name
Anti-Alkaline phosphatase/ALPL Antibody
Gene Names
ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
Topoisomerase II alpha, Polyclonal Antibody (Cat# AAA29961)
Full Name
Topoisomerase II alpha Antibody
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
Application Data
(C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)
FGFR2, Polyclonal Antibody (Cat# AAA26855)
Full Name
FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (Azide free) (HRP)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Monkey, Mouse, Rat
Applications
IHC, EIA, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using ASB3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
ASB3, Polyclonal Antibody (Cat# AAA22266)
Full Name
ASB3 Polyclonal Antibody
Gene Names
ASB3; ASB-3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Anti-Myelin Basic Protein antibody, AAA11509, IHC(P)IHC(P): Rat Brain Tissue)
Myelin Basic Protein, Polyclonal Antibody (Cat# AAA11509)
Full Name
Anti-Myelin Basic Protein antibody
Reactivity
Mouse, Rat
Applications
WB, IHC, IF
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Human Colorectal cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human FLNC AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Filamin C Gamma, Polyclonal Antibody (Cat# AAA20854)
Full Name
Polyclonal Antibody to Filamin C Gamma (FLNC)
Gene Names
FLNC; ABPA; ABPL; FLN2; MFM5; MPD4; RCM5; CMH26; ABP-280; ABP280A
Reactivity
Human
Applications
WB, IHC
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
ICC (Immunocytochemistry)
(ICC staining GAP43 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
GAP43, Polyclonal Antibody (Cat# AAA29963)
Full Name
GAP43 Antibody
Gene Names
Gap43; B-50; Basp2; GAP-43
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
IHC (Immunohistochemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Caveolin 2, Polyclonal Antibody (Cat# AAA31397)
Full Name
Phospho-Caveolin 2 (Ser36) Antibody
Gene Names
CAV2; CAV
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Recoverin/RCVRN using anti-Recoverin/RCVRN antibody (AAA19878).Recoverin/RCVRN was detected in a paraffin-embedded section of human uterus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Recoverin/RCVRN antigen affinity purified polyclonal antibody (#AAA19878) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Recoverin/RCVRN at approximately 26kDa. The expected band size for Recoverin/RCVRN is at 23 kDa.)
Recoverin/RCVRN, Polyclonal Antibody (Cat# AAA19878)
Full Name
Anti-Recoverin/RCVRN Antibody Picoband
Gene Names
RCVRN; RCV1
Reactivity
Human
Applications
WB, IHC, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
IL-1RL2, Polyclonal Antibody (Cat# AAA10970)
Full Name
IL-1RL2 Antibody
Gene Names
IL1RL2; IL-36R; IL1RRP2; IL-1Rrp2; IL1R-rp2
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC-P, WB
Purity
Affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistchemistry)
(Anti-GLP1R/GLP-1R/GLP-1 Receptor antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
GLP1R/GLP-1R/GLP-1 Receptor, Polyclonal Antibody (Cat# AAA12337)
Full Name
Rabbit Polyclonal to Human GLP1R/GLP-1R/GLP-1 Receptor
Reactivity
Gibbon, Gorilla, Human
Predicted Reactivity: Monkey, Mouse, Rat, Bovine, Hamster, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
Predicted Reactivity: Monkey, Mouse, Rat, Bovine, Hamster, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
Applications
IHC - Paraffin
Purity
Immunoaffinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-RPS14 antibody (AAA19798).Overlay histogram showing HepG2 cells stained with AAA19798 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS14 Antibody (AAA19798, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPS14, Polyclonal Antibody (Cat# AAA19798)
Full Name
Anti-RPS14 Antibody Picoband
Gene Names
RPS14; S14; EMTB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, IP, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
Glut1, Polyclonal Antibody (Cat# AAA29030)
Full Name
Glut1 Polyclonal Antibody
Gene Names
SLC2A1; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT1DS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CD274 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
CD274, Polyclonal Antibody (Cat# AAA29874)
Full Name
CD274 (PD1L1) Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HeLa cells using anti-TOLLIP antibody (AAA19444).Overlay histogram showing HeLa cells stained with AAA19444 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOLLIP Antibody (AAA19444, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TOLLIP, Polyclonal Antibody (Cat# AAA19444)
Full Name
Anti-TOLLIP Antibody Picoband
Gene Names
TOLLIP; IL-1RAcPIP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of U937 cells using anti-MPPB/PMPCB antibody (AAA19622).Overlay histogram showing U937 cells stained with AAA19622 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPPB/PMPCB Antibody (AAA19622, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MPPB/PMPCB, Polyclonal Antibody (Cat# AAA19622)
Full Name
Anti-MPPB/PMPCB Antibody Picoband
Gene Names
PMPCB; MPPB; P-52; MPP11; MPPP52; Beta-MPP
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of U-937 cells, using NLRP3 antibody at 1:1000 dilution.)
NLRP3, Polyclonal Antibody (Cat# AAA30558)
Full Name
NLRP3 Polyclonal Antibody
Gene Names
NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; FLJ95925
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Myelin Basic Protein/MBP, Polyclonal Antibody (Cat# AAA31339)
Full Name
Myelin Basic Protein/MBP Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (86%), Xenopus (86%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (86%), Xenopus (86%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using HSF1 at dilution of 1:100 (40x lens).)
HSF1, Polyclonal Antibody (Cat# AAA30618)
Full Name
HSF1 Rabbit Polyclonal Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
ERK 1/2, Polyclonal Antibody (Cat# AAA28926)
Full Name
ERK 1/2 (phospho Tyr204) Polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26894)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, EIA, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
COL1A2, Polyclonal Antibody (Cat# AAA28998)
Full Name
COL1A2 Polyclonal Antibody
Gene Names
COL1A2; OI4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of MCF-7 cells using anti-Pyruvate Carboxylase/PC antibody (AAA19801).Overlay histogram showing MCF-7 cells stained with AAA19801 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Pyruvate Carboxylase/PC Antibody (AAA19801, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Pyruvate Carboxylase/PC, Polyclonal Antibody (Cat# AAA19801)
Full Name
Anti-Pyruvate Carboxylase/PC Antibody Picoband
Gene Names
PC; PCB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Ki-67, Polyclonal Antibody (Cat# AAA29057)
Full Name
Ki-67 Polyclonal Antibody
Gene Names
MKI67; KIA
Reactivity
Human
Applications
WB, IHC-P, IF
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Survivin, Polyclonal Antibody (Cat# AAA29126)
Full Name
Survivin Polyclonal Antibody
Gene Names
BIRC5; API4; EPR-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of MED9 using anti-MED9 antibody (AAA19180).MED9 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED9 Antibody (AAA19180) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
MED9, Polyclonal Antibody (Cat# AAA19180)
Full Name
Anti-MED9 Picoband Antibody
Gene Names
MED9; MED25
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human stomach using MAPK8 antibody at dilution of 1:100 (40x lens).)
MAPK8, Polyclonal Antibody (Cat# AAA10635)
Full Name
MAPK8 Polyclonal Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse
Applications
WB, IF
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of THP-1 cells using anti-LIN7C antibody (AAA19897).Overlay histogram showing THP-1 cells stained with AAA19897 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LIN7C Antibody (AAA19897, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
LIN7C, Polyclonal Antibody (Cat# AAA19897)
Full Name
Anti-LIN7C Antibody Picoband
Gene Names
LIN7C; MALS3; VELI3; LIN-7C; MALS-3; LIN-7-C
Reactivity
Human, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA26968 at dilution of 1:100)
STAT5B, Polyclonal Antibody (Cat# AAA26968)
Full Name
STAT5B Antibody
Gene Names
STAT5B; STAT5
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using ZNF416 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
ZNF416, Polyclonal Antibody (Cat# AAA22185)
Full Name
ZNF416 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Hela cells using anti-ATG9A antibody (AAA19280).Overlay histogram showing Hela cells stained with AAA19280 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG9A Antibody (AAA19280, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATG9A, Polyclonal Antibody (Cat# AAA19280)
Full Name
Anti-ATG9A Antibody
Gene Names
ATG9A; mATG9; APG9L1; MGD3208
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot analysis of mouse brain cells using CD13 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000)
CD13, Polyclonal Antibody (Cat# AAA30760)
Full Name
CD13 Rabbit Polyclonal Antibody
Gene Names
ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
Reactivity
Human, Rat, Mouse
Applications
WB, IHC-P, IF, paraffin section, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry)
(ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ATP5B, Polyclonal Antibody (Cat# AAA28724)
Full Name
ATP5B Antibody (Center)
Gene Names
ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 12. Flow Cytometry analysis of RH35 cells using anti-Elongin-C/ELOC antibody (AAA19646).Overlay histogram showing RH35 cells stained with AAA19646 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Elongin-C/ELOC Antibody (AAA19646, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Elongin-C/ELOC, Polyclonal Antibody (Cat# AAA19646)
Full Name
Anti-Elongin-C/ELOC Antibody Picoband
Gene Names
TCEB1; SIII; eloC
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Human Rectum Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human CRP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.)
C Reactive Protein (CRP), Polyclonal Antibody (Cat# AAA20108)
Full Name
Polyclonal Antibody to C Reactive Protein (CRP)
Gene Names
CRP; PTX1
Reactivity
Human, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Cytokeratin 8, Polyclonal Antibody (Cat# AAA29221)
Full Name
Cytokeratin 8 Polyclonal Antibody
Gene Names
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity
Human
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Amphiphysin II, Polyclonal Antibody (Cat# AAA29230)
Full Name
Amphiphysin II Polyclonal Antibody
Gene Names
BIN1; AMPH2; AMPHL; SH3P9
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of MCF-7 cells using AAA18542 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Glyceraldehyde-3-phosphate dehydrogenase, Polyclonal Antibody (Cat# AAA18542)
Full Name
Rabbit anti-human Glyceraldehyde-3-phosphate dehydrogenase polyclonal Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
IF (Immunofluorescence)
(Figure 10 Immunofluorescence images of PUMA in the P14 rat retina (Wakabayashi et al., 2012)PUMA expression in the rat retina detected by anti-PUMA antibodies (3043). The specimens were counterstained with Hoechst 33258 to visualize nuclei (+DNA). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; P, postnatal day.)
PUMA, Polyclonal Antibody (Cat# AAA10934)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
PUMA Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Prothrombin, Polyclonal Antibody (Cat# AAA29149)
Full Name
Prothrombin Polyclonal Antibody
Gene Names
F2; PT; THPH1; RPRGL2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Mouse Heart Tissue)
Peptidylprolyl Isomerase F (PPIF), Polyclonal Antibody (Cat# AAA20314)
Full Name
Polyclonal Antibody to Peptidylprolyl Isomerase F (PPIF)
Gene Names
Ppif; CypD; CyP-D; CyP-F; PPIase; AW457192
Reactivity
Mouse, Human, Rat
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: ASIC1Sample Type: MCF7 Whole Cell lysatesAntibody Dilution: 1.0ug/ml)
ACCN2, Polyclonal Antibody (Cat# AAA23442)
Full Name
ACCN2 antibody - N-terminal region
Gene Names
ASIC1; ASIC; ACCN2; BNaC2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with PARK2 (Parkin) antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Parkin, Polyclonal Antibody (Cat# AAA28668)
Full Name
Parkin Antibody (C-term)
Gene Names
PARK2; PDJ; PRKN; AR-JP; LPRS2
Reactivity
Human, mouse
Applications
WB, EIA, IF, FC/FACS, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26896)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, EIA, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.