Polyclonal Antibodies

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-thyroid, antibody was diluted at 1:200)

Thyroglobulin, Polyclonal Antibody (Cat# AAA30767)

Full Name

Thyroglobulin Rabbit Polyclonal Antibody

Gene Names

TG; TGN; AITD3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

PACSIN1, Polyclonal Antibody (Cat# AAA28726)

Full Name

PACSIN1 Antibody (N-term)

Gene Names

PACSIN1; SDPI

Reactivity

Human, mouse, rat (Predicted Reactivity: Rat, Chicken, Bovine, Xenopus)

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Caco-2 cells using anti-MATK antibody (AAA19767).Overlay histogram showing Caco-2 cells stained with AAA19767 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MATK Antibody (AAA19767, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of MATK using anti-MATK antibody (AAA19767).MATK was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse thymus tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATK antigen affinity purified polyclonal antibody (#AAA19767) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MATK at approximately 52 kDa. The expected band size for MATK is at 56 kDa.)

MATK, Polyclonal Antibody (Cat# AAA19767)

Full Name

Anti-MATK Antibody Picoband

Gene Names

MATK; CHK; CTK; HYL; Lsk; HYLTK; HHYLTK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

TOM20, Polyclonal Antibody (Cat# AAA22327)

Full Name

(KD Validated) TOM20 Polyclonal Antibody

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Host: RabbitTarget Name: GBRPSample Type: Jurkat Whole Cell lysatesAntibody Dilution: 1ug/ml)

GABRP, Polyclonal Antibody (Cat# AAA23607)

Full Name

GABRP antibody - N-terminal region

Reactivity

Dog, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 antibody at dilution of 1:100 (40x lens).)

CDH16, Polyclonal Antibody (Cat# AAA28211)

Full Name

CDH16 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (SYP Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

SYP, Polyclonal Antibody (Cat# AAA28723)

Full Name

SYP Antibody (C-term)

Gene Names

SYP; MRX96; MRXSYP

Reactivity

Human, mouse (Predicted Reactivity: Rat)

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Catenin-alpha1, Polyclonal Antibody (Cat# AAA28979)

Full Name

Catenin-alpha1 Polyclonal Antibody

Gene Names

CTNNA1; CAP102

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin (IHC-P)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of K562 cells using anti-PSIP1 antibody (AAA19764).Overlay histogram showing K562 cells stained with AAA19764 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSIP1 Antibody (AAA19764, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PSIP1 using anti-PSIP1 antibody (AAA19764) and anti-Beta Tubulin antibody (M01857-3).PSIP1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSIP1 Antibody (AAA19764) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSIP1 Antibody (AAA19764) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSIP1 Antibody (AAA19764) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSIP1 Antibody (AAA19764) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSIP1 Antibody (AAA19764) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSIP1 Antibody (AAA19764) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human HEL whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: rat PC-12 whole cell lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSIP1 antigen affinity purified polyclonal antibody (#AAA19764) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSIP1 at approximately 85 kDa. The expected band size for PSIP1 is at 60 kDa.)

PSIP1, Polyclonal Antibody (Cat# AAA19764)

Full Name

Anti-PSIP1 Antibody Picoband

Gene Names

PSIP1; p52; p75; PAIP; DFS70; LEDGF; PSIP2

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using Caspase-3 p17 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Caspase-3 p17, Polyclonal Antibody (Cat# AAA22324)

Full Name

(KO Validated) Caspase-3 p17 Polyclonal Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of SRC Antibody (N-term) with A431 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

SRC, Polyclonal Antibody (Cat# AAA28721)

Full Name

SRC Antibody (N-term)

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Caspase-8, Polyclonal Antibody (Cat# AAA28977)

Full Name

Caspase-8 Polyclonal Antibody

Gene Names

CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (PRKAR1A Antibody-Immunohistochemistry of paraffin-embedded human esophageal cancer using PRKAR1A antibody at dilution of 1:100 (200x lens).)

PRKAR1A, Polyclonal Antibody (Cat# AAA21298)

Full Name

PRKAR1A Rabbit anti-Human Polyclonal Antibody

Gene Names

PRKAR1A; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1

Reactivity

Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

< >

<< Previous Next >>

WB (Western Blot) (Anti-tdTomato Ab conjugated to DyLight 550 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane;)

tdTomato, Polyclonal Antibody (Cat# AAA13880)

Full Name

Anti-tdTomato, DyLight550

Reactivity

Reacts with Transfected cells proteins

Applications

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Frozen (IHC-F)

Purity

This antibody is epitope-affinity purified from goat antiserum.

Pricing

Goat Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14904)

Full Name

Goat Anti-Human IgG-HRP

Applications

ELISA

Purity

Affinity chromatography on human IgG covalently linked to agarose

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 5. Flow Cytometry analysis of HepG2 cells using anti-PGM3 antibody (AAA19768).Overlay histogram showing HepG2 cells stained with AAA19768 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGM3 Antibody (AAA19768, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 4. IF analysis of PGM3 using anti-PGM3 antibody (AAA19768) and anti-Beta Tubulin antibody (M01857-3).PGM3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGM3 Antibody (AAA19768) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGM3 Antibody (AAA19768) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human k562 whole cell lysates,Lane 5: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGM3 antigen affinity purified polyclonal antibody (#AAA19768) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PGM3 at approximately 63 kDa. The expected band size for PGM3 is at 60 kDa.)

PGM3, Polyclonal Antibody (Cat# AAA19768)

Full Name

Anti-PGM3 Antibody Picoband

Gene Names

PGM3; AGM1; PAGM; IMD23; PGM 3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)

FGFR2, Polyclonal Antibody (Cat# AAA28728)

Full Name

FGFR2 Antibody (N-term)

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, mouse, rat, monkey

Applications

ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

CD34, Polyclonal Antibody (Cat# AAA28984)

Full Name

CD34 Polyclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Dilution: IF: 1:50-200 WB 1:500-2000, IHC 1:50-300)

CD44, Polyclonal Antibody (Cat# AAA28985)

Full Name

CD44 Polyclonal Antibody

Gene Names

CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of Jurkat cells using Acetyl-Ku-70 (K542) Polyclonal Antibody. Secondary antibody was diluted at 1:20000)

Ku-70, Polyclonal Antibody (Cat# AAA30777)

Full Name

Ku-70 (Acetyl-Lys542) Rabbit Polyclonal Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human, Rat, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Anti-CD63 Antibody (C-term)at 1:2000 dilution + human plasma lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

CD63, Polyclonal Antibody (Cat# AAA28725)

Full Name

CD63 Antibody (C-term)

Gene Names

CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Laminin gamma-2, Polyclonal Antibody (Cat# AAA29237)

Full Name

Laminin gamma-2 Polyclonal Antibody

Gene Names

LAMC2; B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Cathepsin B, Polyclonal Antibody (Cat# AAA28980)

Full Name

Cathepsin B Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of HT-29 cells using PROM1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

PROM1, Polyclonal Antibody (Cat# AAA22325)

Full Name

(KD Validated) PROM1 Polyclonal Antibody

Gene Names

PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using C9orf72 Rabbit pAb (AAA28290) at dilution of 1:100 (40x lens).)

C9orf72, Polyclonal Antibody (Cat# AAA28290)

Full Name

C9orf72 Polyclonal Antibody

Gene Names

C9orf72; ALSFTD; DENND9; FTDALS; DENNL72; FTDALS1

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

KiSS-1R, Polyclonal Antibody (Cat# AAA29058)

Full Name

KiSS-1R Polyclonal Antibody

Gene Names

KISS1R; HH8; GPR54; AXOR12; KISS-1R; HOT7T175

Reactivity

Human

Applications

Immunofluorescence (IF)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using KAT2A Polyclonal Antibody.)

KAT2A, Polyclonal Antibody (Cat# AAA29826)

Full Name

KAT2A Polyclonal Antibody

Gene Names

KAT2A; GCN5; hGCN5; GCN5L2; PCAF-b

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Figure 7. IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (AAA19843).FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (AAA19843) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (AAA19843) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOX2/RBM9/RBFOX2 Antibody (AAA19843, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (AAA19843).FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (AAA19843) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (AAA19843) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (AAA19843) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: human U-87MG whole cell lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOX2/RBM9/RBFOX2 antigen affinity purified polyclonal antibody (#AAA19843) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FOX2/RBM9/RBFOX2 at approximately 60 kDa. The expected band size for FOX2/RBM9/RBFOX2 is at 41 kDa.)

FOX2/RBM9/RBFOX2, Polyclonal Antibody (Cat# AAA19843)

Full Name

Anti-FOX2/RBM9/RBFOX2 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (HSPA5/GRP78/BiP Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

HSPA5/GRP78/BiP, Polyclonal Antibody (Cat# AAA21379)

Full Name

HSPA5/GRP78/BiP Goat anti-Human Polyclonal Antibody

Gene Names

HSPA5; BIP; MIF2; GRP78

Reactivity

Human

Applications

Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

CD72, Polyclonal Antibody (Cat# AAA29321)

Full Name

CD72 Polyclonal Antibody

Gene Names

CD72; LYB2; CD72b

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using STAT1 at dilution of 1:100 (40x lens).)

STAT1, Polyclonal Antibody (Cat# AAA30601)

Full Name

STAT1 Rabbit Polyclonal Antibody

Gene Names

STAT1; CANDF7; ISGF-3; STAT91

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of PC-3 cells using anti-ATG14L antibody (AAA11658).Overlay histogram showing PC-3 cells stained with AAA11658 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14L Antibody (AAA11658,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ATG14L, Polyclonal Antibody (Cat# AAA11658)

Full Name

Anti-ATG14L Antibody

Gene Names

ATG14; ATG14L; BARKOR; KIAA0831

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen Affinity Purified

Pricing

< >

<< Previous Next >>

DB (Dot Blot) (Dot blot analysis using Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507) at 1:500, 1:5000. Beta Amyloid HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.)

Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17802)

Full Name

Amyloid Fibrils (OC) Antibody: RPE

Reactivity

Human. Potentially mouse and rat based on species homology.

Applications

Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB), Dot Blot (DB)

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of MCF-7 cells using anti-PAPSS2 antibody (AAA19850).Overlay histogram showing MCF-7 cells stained with AAA19850 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAPSS2 Antibody (AAA19850, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PAPSS2 using anti-PAPSS2 antibody (AAA19850).PAPSS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PAPSS2 Antibody (AAA19850) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PAPSS2 Antibody (AAA19850) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PAPSS2 Antibody (AAA19850) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PAPSS2 Antibody (AAA19850) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat liver tissue lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse liver tissue lysates,Lane 5: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAPSS2 antigen affinity purified polyclonal antibody (#AAA19850) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAPSS2 at approximately 70 kDa. The expected band size for PAPSS2 is at 70 kDa.)

PAPSS2, Polyclonal Antibody (Cat# AAA19850)

Full Name

Anti-PAPSS2 Antibody Picoband

Gene Names

PAPSS2; SK2; BCYM4; ATPSK2

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-SUV420H1 AntibodyTitration: 1.25 ug/mlPositive Control: Jurkat Whole Cell)

KMT5B, Polyclonal Antibody (Cat# AAA23434)

Full Name

KMT5B Antibody - middle region

Gene Names

KMT5B; CGI85; MRD51; CGI-85; SUV420H1

Reactivity

Dog, Guinea Pig, Horse, Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

TP53INP2, Polyclonal Antibody (Cat# AAA29324)

Full Name

TP53INP2 Polyclonal Antibody

Gene Names

TP53INP2; DOR; PINH; FLJ21759; FLJ23500; C20orf110; dJ1181N3.1; DKFZp434B2411; DKFZp434O0827

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse testis using AKT2 at dilution of 1:200 (40x lens).)

Akt2, Polyclonal Antibody (Cat# AAA30604)

Full Name

Akt2 Rabbit Polyclonal Antibody

Gene Names

AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A549 cells using anti-NIT2 antibody (AAA19853).Overlay histogram showing A549 cells stained with AAA19853 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NIT2 Antibody (AAA19853, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of NIT2 using anti-NIT2 antibody (AAA19853).NIT2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat liver tissue lysates,Lane 3: rat kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NIT2 antigen affinity purified polyclonal antibody (#AAA19853) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NIT2 at approximately 33 kDa. The expected band size for NIT2 is at 33 kDa.)

NIT2, Polyclonal Antibody (Cat# AAA19853)

Full Name

Anti-NIT2 Antibody Picoband

Gene Names

NIT2; HEL-S-8a

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-CREB3L2 Antibody Titration: 1.25ug/mlELISA Titer: 1:12500Positive Control: Jurkat cell lysate)

CREB3L2, Polyclonal Antibody (Cat# AAA23437)

Full Name

CREB3L2 antibody - N-terminal region

Gene Names

CREB3L2; BBF2H7

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Protein A purified

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry-DEPDC6 Polyclonal Antibody)

DEPDC6, Polyclonal Antibody (Cat# AAA28301)

Full Name

DEPDC6 Polyclonal Antibody

Gene Names

DEPTOR; DEP.6; DEPDC6

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (BPTF) Polyclonal Antibody, Unconjugated (bs-11641R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

BPTF, Polyclonal Antibody (Cat# AAA28813)

Full Name

BPTF Polyclonal Antibody

Gene Names

BPTF; FAC1; FALZ; NURF301

Reactivity

Rat, Dog, Cow

Applications

Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using UBE2L3 Polyclonal Antibody.)

UBE2L3, Polyclonal Antibody (Cat# AAA29829)

Full Name

UBE2L3 Polyclonal Antibody

Gene Names

UBE2L3; E2-F1; L-UBC; UBCH7; UbcM4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] HIF1?? Rabbit pAb.)

HIF1A, Polyclonal Antibody (Cat# AAA30597)

Full Name

HIF1A Rabbit Polyclonal Antibody

Gene Names

HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using MCL1 antibody at dilution of 1:100 (40x lens).)

MCL1, Polyclonal Antibody (Cat# AAA10630)

Full Name

MCL1 Polyclonal Antibody

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using AAA14726 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Heat Shock Protein 27, Polyclonal Antibody (Cat# AAA14726)

Full Name

Heat Shock Protein 27, phosphorylated (Ser82) (HSP27)

Gene Names

Hsp27; 27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by protein A and peptide affinity chromatography.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of 293T cells using anti-NVL antibody (AAA19846).Overlay histogram showing 293T cells stained with AAA19846 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NVL Antibody (AAA19846, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of NVL using anti-NVL antibody (AAA19846) and anti-Beta Tubulin antibody (M01857-3).NVL was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NVL antigen affinity purified polyclonal antibody (#AAA19846) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NVL at approximately 95 kDa. The expected band size for NVL is at 95 kDa.)

NVL, Polyclonal Antibody (Cat# AAA19846)

Full Name

Anti-NVL Antibody Picoband

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

MKP-1/2, Polyclonal Antibody (Cat# AAA29069)

Full Name

MKP-1/2 Polyclonal Antibody

Gene Names

DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using DNAJB6 antibody.)

DNAJB6, Polyclonal Antibody (Cat# AAA29837)

Full Name

DNAJB6 Polyclonal Antibody

Gene Names

DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

< >

Clcn5, Polyclonal Antibody (Cat# AAA23438)

Full Name

Clcn5 antibody - middle region

Gene Names

Clcn5; Clc5; ClC-5; Sfc13; Clc4-1; T25545; Clcn4-1; DXImx42e; 5430408K11Rik; D930009B12Rik

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using SIRT2 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

SIRT2, Polyclonal Antibody (Cat# AAA30606)

Full Name

SIRT2 Rabbit Polyclonal Antibody

Gene Names

SIRT2; SIR2; SIR2L; SIR2L2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

Loading...

Navigation

Viewing 200-250 of 3252 product results

Thyroglobulin, Polyclonal Antibody (Cat# AAA30767)

PACSIN1, Polyclonal Antibody (Cat# AAA28726)

MATK, Polyclonal Antibody (Cat# AAA19767)

TOM20, Polyclonal Antibody (Cat# AAA22327)

GABRP, Polyclonal Antibody (Cat# AAA23607)

CDH16, Polyclonal Antibody (Cat# AAA28211)

SYP, Polyclonal Antibody (Cat# AAA28723)

Catenin-alpha1, Polyclonal Antibody (Cat# AAA28979)

PSIP1, Polyclonal Antibody (Cat# AAA19764)

Caspase-3 p17, Polyclonal Antibody (Cat# AAA22324)

SRC, Polyclonal Antibody (Cat# AAA28721)

Caspase-8, Polyclonal Antibody (Cat# AAA28977)

PRKAR1A, Polyclonal Antibody (Cat# AAA21298)

tdTomato, Polyclonal Antibody (Cat# AAA13880)

Goat Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14904)

PGM3, Polyclonal Antibody (Cat# AAA19768)

FGFR2, Polyclonal Antibody (Cat# AAA28728)

CD34, Polyclonal Antibody (Cat# AAA28984)

CD44, Polyclonal Antibody (Cat# AAA28985)

Ku-70, Polyclonal Antibody (Cat# AAA30777)

CD63, Polyclonal Antibody (Cat# AAA28725)

Laminin gamma-2, Polyclonal Antibody (Cat# AAA29237)

Cathepsin B, Polyclonal Antibody (Cat# AAA28980)

PROM1, Polyclonal Antibody (Cat# AAA22325)

C9orf72, Polyclonal Antibody (Cat# AAA28290)

KiSS-1R, Polyclonal Antibody (Cat# AAA29058)

KAT2A, Polyclonal Antibody (Cat# AAA29826)

FOX2/RBM9/RBFOX2, Polyclonal Antibody (Cat# AAA19843)

HSPA5/GRP78/BiP, Polyclonal Antibody (Cat# AAA21379)

CD72, Polyclonal Antibody (Cat# AAA29321)

STAT1, Polyclonal Antibody (Cat# AAA30601)

ATG14L, Polyclonal Antibody (Cat# AAA11658)

Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17802)

PAPSS2, Polyclonal Antibody (Cat# AAA19850)

KMT5B, Polyclonal Antibody (Cat# AAA23434)

TP53INP2, Polyclonal Antibody (Cat# AAA29324)

Akt2, Polyclonal Antibody (Cat# AAA30604)

NIT2, Polyclonal Antibody (Cat# AAA19853)

CREB3L2, Polyclonal Antibody (Cat# AAA23437)

DEPDC6, Polyclonal Antibody (Cat# AAA28301)

BPTF, Polyclonal Antibody (Cat# AAA28813)

UBE2L3, Polyclonal Antibody (Cat# AAA29829)

HIF1A, Polyclonal Antibody (Cat# AAA30597)

MCL1, Polyclonal Antibody (Cat# AAA10630)

Heat Shock Protein 27, Polyclonal Antibody (Cat# AAA14726)

NVL, Polyclonal Antibody (Cat# AAA19846)

MKP-1/2, Polyclonal Antibody (Cat# AAA29069)

DNAJB6, Polyclonal Antibody (Cat# AAA29837)

Clcn5, Polyclonal Antibody (Cat# AAA23438)

SIRT2, Polyclonal Antibody (Cat# AAA30606)

Contact us by

Submit a Question

Request more Information

Request a Manual

Request a Quote