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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 200-250 of 10000 product results
WB (Western Blot)
(Western blot analysis of extracts of various sample, using Catenin-beta antibody.)
Catenin-beta, Polyclonal Antibody (Cat# AAA31090)
Full Name
Catenin-beta Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26896)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IF (Immunofluorescence)
(Figure 13. IF analysis of STIM1 using anti-STIM1 antibody (AAA19731).STIM1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
STIM1, Polyclonal Antibody (Cat# AAA19731)
Full Name
Anti-STIM1 Antibody Picoband
Gene Names
STIM1; GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)
DRP1, Polyclonal Antibody (Cat# AAA30750)
Full Name
DRP1 Rabbit Polyclonal Antibody
Gene Names
DNM1L; DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
ICC (Immunocytochemistry)
(ICC staining MGMT in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
MGMT, Polyclonal Antibody (Cat# AAA29958)
Full Name
MGMT Antibody
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC12 cells using EGFR antibody.)
EGFR, Polyclonal Antibody (Cat# AAA29798)
Full Name
EGFR Polyclonal Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
PDGF-B, Polyclonal Antibody (Cat# AAA29094)
Full Name
PDGF-B Polyclonal Antibody
Gene Names
PDGFB; SIS; SSV; PDGF2; c-sis; PDGF-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
MIP-3alpha, Polyclonal Antibody (Cat# AAA29367)
Full Name
MIP-3alpha Polyclonal Antibody
Gene Names
CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p70 S6 Kinase, Polyclonal Antibody (Cat# AAA31408)
Full Name
Phospho-p70 S6 Kinase (Ser427) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
MAP3K3, Polyclonal Antibody (Cat# AAA31283)
Full Name
Phospho-MAP3K3 (Ser166) Antibody
Gene Names
MAP3K3; MEKK3; MAPKKK3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)
PTN, Polyclonal Antibody (Cat# AAA29284)
Full Name
PTN Polyclonal Antibody
Gene Names
PTN; HARP; HBNF; HBGF8; NEGF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Western blot analysis of extracts from 3T3 cells treated with EGF, HWEC cells treated with VEGF and JK cells using eNOS (Phospho-Ser1177) Antibody #AAA29721.)
eNOS, Polyclonal Antibody (Cat# AAA29721)
Full Name
eNOS (Phospho-Ser1177) Antibody
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26878)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 490)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31354)
Full Name
Acetyl-p53 (Lys373) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
AXL, Polyclonal Antibody (Cat# AAA31469)
Full Name
Phospho-AXL (Tyr702) Antibody
Gene Names
AXL; UFO; JTK11
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
ATP1A1, Polyclonal Antibody (Cat# AAA31454)
Full Name
Phospho-ATP1A1 (Tyr260) Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%), Xenopus (90%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%), Xenopus (90%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells usingug CDK4 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK4 Polyclonal Antibody at a dilution of 1:1000.)
CDK4, Polyclonal Antibody (Cat# AAA22305)
Full Name
(KO Validated) CDK4 Polyclonal Antibody
Gene Names
CDK4; CMM3; PSK-J3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using CCDC59 antibody.)
CCDC59, Polyclonal Antibody (Cat# AAA29808)
Full Name
CCDC59 Polyclonal Antibody
Gene Names
CCDC59; BR22; TAP26; HSPC128
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse IFNa AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Interferon Alpha, Polyclonal Antibody (Cat# AAA21018)
Full Name
Polyclonal Antibody to Interferon Alpha (IFNa)
Gene Names
Ifna1; Ifa1
Reactivity
Mouse
Applications
Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using DAPK3 antibody at dilution of 1:100 .)
DAPK3, Polyclonal Antibody (Cat# AAA30573)
Full Name
DAPK3 Polyclonal Antibody
Gene Names
DAPK3; ZIP; ZIPK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of SH-SY5Y cells using anti-Lumican/LUM antibody (AAA19744).Overlay histogram showing SH-SY5Y cells stained with AAA19744 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Lumican/LUM Antibody (AAA19744, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Lumican/LUM, Polyclonal Antibody (Cat# AAA19744)
Full Name
Anti-Lumican/LUM Antibody Picoband
Gene Names
LUM; LDC; SLRR2D
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Lamin A/C, Polyclonal Antibody (Cat# AAA22292)
Full Name
Lamin A/C Polyclonal Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using MAP1LC3B antibody at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.)
MAP1LC3B, Polyclonal Antibody (Cat# AAA28283)
Full Name
MAP1LC3B Polyclonal Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Overlay histogram showing U251 cells stained with AAA19481 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Acid Phosphatase/ACP5 Antibody (AAA19481, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Acid phosphatase/ACP5, Polyclonal Antibody (Cat# AAA19481)
Full Name
Anti-Acid phosphatase/ACP5 Antibody Picoband
Gene Names
ACP5; TRAP; SPENCDI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
PTEN, Polyclonal Antibody (Cat# AAA28948)
Full Name
PTEN (phospho Ser380/T382/T383) Polyclonal Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Daudi cells using anti-Ubc13/UBE2N antibody (AAA19458).Overlay histogram showing Daudi cells stained with AAA19458 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ubc13/UBE2N Antibody (AAA19458, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Ubc13/UBE2N, Polyclonal Antibody (Cat# AAA19458)
Full Name
Anti-Ubc13/UBE2N Antibody Picoband
Gene Names
UBE2N; UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SAMHD1, Polyclonal Antibody (Cat# AAA31278)
Full Name
Phospho-SAMHD1 (Thr592) Antibody
Gene Names
SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26872)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (AP)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-CGKI/PRKG1 antibody (AAA19246).Overlay histogram showing U251 cells stained with AAA19246 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGKI/PRKG1 Antibody (AAA19246, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
cGKI/PRKG1, Polyclonal Antibody (Cat# AAA19246)
Full Name
Anti-cGKI/PRKG1 Antibody
Gene Names
PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines.)
PSMA5, Polyclonal Antibody (Cat# AAA21278)
Full Name
Anti-PSMA5 Antibody
Gene Names
PSMA5; PSC5; ZETA
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
JIP-3, Polyclonal Antibody (Cat# AAA29055)
Full Name
JIP-3 Polyclonal Antibody
Gene Names
MAPK8IP3; syd; JIP3; SYD2; JSAP1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using RUNX1 antibody. Blue: DAPI for nuclear staining.)
RUNX1, Polyclonal Antibody (Cat# AAA10677)
Full Name
RUNX1 Polyclonal Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using SFPQ antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
SFPQ, Polyclonal Antibody (Cat# AAA10716)
Full Name
SFPQ Polyclonal Antibody
Gene Names
SFPQ; PSF; POMP100; PPP1R140
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Smad2, Polyclonal Antibody (Cat# AAA31390)
Full Name
Phospho-Smad2 (Ser255) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-HRP.)
Goat Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14904)
Full Name
Goat Anti-Human IgG-HRP
Applications
ELISA
Purity
Affinity chromatography on human IgG covalently linked to agarose
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CD58, Polyclonal Antibody (Cat# AAA29194)
Full Name
CD58 Polyclonal Antibody
Gene Names
CD58; ag3; LFA3; LFA-3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)
Glut4, Polyclonal Antibody (Cat# AAA29286)
Full Name
Glut4 Polyclonal Antibody
Gene Names
SLC2A4; GLUT4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using FLNB antibody at dilution of 1:100 (40x lens).)
FLNB, Polyclonal Antibody (Cat# AAA10740)
Full Name
FLNB Polyclonal Antibody
Gene Names
FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IRF7, Polyclonal Antibody (Cat# AAA31458)
Full Name
Phospho-IRF7 (Ser477) Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 70 kDa isoform is identified, and a second isoform of 65 kDa is also present in some samples.)
SLC27A2, Polyclonal Antibody (Cat# AAA23511)
Full Name
SLC27A2 antibody - N-terminal region
Gene Names
SLC27A2; VLCS; FATP2; VLACS; ACSVL1; FACVL1; hFACVL1; HsT17226
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Anti-GLP1R/GLP-1R/GLP-1 Receptor antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
GLP1R/GLP-1R/GLP-1 Receptor, Polyclonal Antibody (Cat# AAA12337)
Full Name
Rabbit Polyclonal to Human GLP1R/GLP-1R/GLP-1 Receptor
Reactivity
Gibbon, Gorilla, Human
Predicted Reactivity: Monkey, Mouse, Rat, Bovine, Hamster, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
Predicted Reactivity: Monkey, Mouse, Rat, Bovine, Hamster, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26895)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating GARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26911 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26911 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
Annexin A2, Polyclonal Antibody (Cat# AAA26911)
Full Name
Rabbit anti-human Annexin A2 polyclonal Antibody
Gene Names
ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
Vimentin, Polyclonal Antibody (Cat# AAA28432)
Full Name
Vimentin Rabbit pAb
Gene Names
ABCA12; LI2; ICR2B; ARCI4A; ARCI4B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Survivin, Polyclonal Antibody (Cat# AAA29126)
Full Name
Survivin Polyclonal Antibody
Gene Names
BIRC5; API4; EPR-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26877)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 405)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using NF-kB p65 Rabbit pAb.)
NF-kB p65, Polyclonal Antibody (Cat# AAA30722)
Full Name
NF-kB p65 Rabbit Polyclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(Human alpha-2 Macroglobulin detected with Sheep anti alpha-2 Macroglobulin:HRP)
ALPHA 2 MACROGLOBULIN, Polyclonal Antibody (Cat# AAA12240)
Full Name
SHEEP ANTI HUMAN ALPHA 2 MACROGLOBULIN
Gene Names
A2M; A2MD; CPAMD5; FWP007; S863-7
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)
NOS2, Polyclonal Antibody (Cat# AAA29081)
Full Name
NOS2 Polyclonal Antibody
Gene Names
NOS2; NOS; INOS; NOS2A; HEP-NOS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat brain using RAB11ARAB11B Rabbit pAb.)
RAB11A/RAB11B, Polyclonal Antibody (Cat# AAA30731)
Full Name
RAB11A/RAB11B Rabbit Polyclonal Antibody
Gene Names
RAB11A; YL8
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.