Polyclonal Antibodies

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WB (Western Blot) (Western Blot analysis of HepG2 cells using SPTLC1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)

SPTLC1, Polyclonal Antibody (Cat# AAA30758)

Full Name

SPTLC1 Rabbit Polyclonal Antibody

Gene Names

SPTLC1; HSN1; LBC1; LCB1; SPT1; SPTI; HSAN1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-APLP2 antibody (AAA19746).Overlay histogram showing HepG2 cells stained with AAA19746 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APLP2 Antibody (AAA19746, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of APLP2 using anti-APLP2 antibody (AAA19746).APLP2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APLP2 antigen affinity purified polyclonal antibody (#AAA19746) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APLP2 at approximately 100-110 kDa. The expected band size for APLP2 is at 87 kDa.)

APLP2, Polyclonal Antibody (Cat# AAA19746)

Full Name

Anti-APLP2 Antibody Picoband

Gene Names

APLP2; APPH; APPL2; CDEBP; APLP-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U20S cells using anti-LRRN4 antibody (AAA20002).Overlay histogram showing U20S cells stained with AAA20002 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LRRN4 Antibody (AAA20002, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of LRRN4 using anti-LRRN4 antibody (AAA20002).LRRN4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U20S whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRN4 antigen affinity purified polyclonal antibody (#AAA20002) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRN4 at approximately 79 kDa. The expected band size for LRRN4 is at 79 kDa.)

LRRN4, Polyclonal Antibody (Cat# AAA20002)

Full Name

Anti-LRRN4 Antibody Picoband

Gene Names

LRRN4; NLRR4; NLRR-4; C20orf75; dJ1056H1.1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.)

CDK2, Polyclonal Antibody (Cat# AAA22306)

Full Name

(KO Validated) CDK2 Polyclonal Antibody

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-RARS/RARS1 antibody (AAA19748).Overlay histogram showing MCF-7 cells stained with AAA19748 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RARS/RARS1 Antibody (AAA19748, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of RARS/RARS1 using anti-RARS/RARS1 antibody (AAA19748) and anti-Tubulin Alpha antibody (M03989-3).RARS/RARS1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RARS/RARS1 Antibody (AAA19748) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RARS/RARS1 Antibody (AAA19748) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RARS/RARS1 Antibody (AAA19748) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARS/RARS1 antigen affinity purified polyclonal antibody (#AAA19748) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RARS/RARS1 at approximately 70 kDa. The expected band size for RARS/RARS1 is at 75 kDa.)

RARS/RARS1, Polyclonal Antibody (Cat# AAA19748)

Full Name

Anti-RARS/RARS1 Antibody Picoband

Gene Names

RARS; HLD9; ArgRS; DALRD1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 7. IF analysis of MAP6D1 using anti-MAP6D1 antibody (AAA20004).MAP6D1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP6D1 Antibody (AAA20004, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of MAP6D1 using anti-MAP6D1 antibody (AAA20004).MAP6D1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP6D1 Antibody (AAA20004) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP6D1 Antibody (AAA20004) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP6D1 Antibody (AAA20004) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP6D1 antigen affinity purified polyclonal antibody (#AAA20004) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP6D1 at approximately 21 kDa. The expected band size for MAP6D1 is at 21 kDa.)

MAP6D1, Polyclonal Antibody (Cat# AAA20004)

Full Name

Anti-MAP6D1 Antibody Picoband

Gene Names

MAP6D1; SL21; MAPO6D1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-ADRM1 antibodyTitration: 1 ug/mlPositive Control: HeLa cells and cytosol from male mouse liver)

ADRM1, Polyclonal Antibody (Cat# AAA23588)

Full Name

ADRM1 antibody - C-terminal region

Gene Names

ADRM1; ARM1; ARM-1; GP110

Reactivity

Guinea Pig, Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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Endoglin, Polyclonal Antibody (Cat# AAA30754)

Full Name

Endoglin Rabbit Polyclonal Antibody

Gene Names

ENG; END; ORW; HHT1; ORW1; CD105

Reactivity

Human, Rat, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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WB (Western Blot) (Western blot of Latexin (arrow) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2))

Latexin / MUM, Polyclonal Antibody (Cat# AAA12323)

Full Name

Rabbit Polyclonal to Human Latexin / MUM

Gene Names

LXN; ECI; TCI

Reactivity

Human

Applications

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunoaffinity Purified

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Cytokeratin 8, Polyclonal Antibody (Cat# AAA29221)

Full Name

Cytokeratin 8 Polyclonal Antibody

Gene Names

KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Figure 7: Immunohistochemistry Validation of NCOA4 in Rat lungImmunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (AAA11046) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

NCOA4, Polyclonal Antibody (Cat# AAA11046)

Full Name

NCOA4 (IN) Antibody

Gene Names

NCOA4; RFG; ELE1; PTC3; ARA70

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF)

Purity

NCOA4 Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

EPAS-1, Polyclonal Antibody (Cat# AAA29219)

Full Name

EPAS-1 Polyclonal Antibody

Gene Names

EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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EGF, Polyclonal Antibody (Cat# AAA30755)

Full Name

EGF Rabbit Polyclonal Antibody

Gene Names

EGF; URG; HOMG4

Reactivity

Human, Rat, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IHC (Immunohistochemistry) (Figure 7: Immunohistochemistry Validation of AIFM2 in Rat TestisImmunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AIFM2 antibody (AAA11044) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

AIFM2, Polyclonal Antibody (Cat# AAA11044)

Full Name

AIFM2 (CT) Antibody

Gene Names

AIFM2; AMID; PRG3; RP11-367H5.2

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

AIMF2 Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistchemistry) (MGP/Matrix Gla-Protein Antibody-Immunohistochemistry of paraffin-embedded rat brain using MGP antibody at dilution of 1:100 (40x lens).)

MGP/Matrix Gla-Protein, Polyclonal Antibody (Cat# AAA21296)

Full Name

MGP/Matrix Gla-Protein Rabbit anti-Human Polyclonal Antibody

Gene Names

MGP; NTI; GIG36; MGLAP

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using CHRFAM7A antibody at dilution of 1:100 (40x lens).)

CHRFAM7A, Polyclonal Antibody (Cat# AAA28208)

Full Name

CHRFAM7A Polyclonal Antibody

Gene Names

CHRFAM7A; D-10; CHRNA7; CHRNA7-DR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.)

Caspase-7, Polyclonal Antibody (Cat# AAA28976)

Full Name

Caspase-7 Polyclonal Antibody

Gene Names

CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

MMP-9, Polyclonal Antibody (Cat# AAA29232)

Full Name

MMP-9 Polyclonal Antibody

Gene Names

MMP9; GELB; CLG4B; MMP-9; MANDP2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200)

Napsin A, Polyclonal Antibody (Cat# AAA30768)

Full Name

Napsin A Rabbit Polyclonal Antibody

Gene Names

NAPSA; KAP; Kdap; NAP1; NAPA; SNAPA

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IHC (Immunohistchemistry) (Anti-GLP1R/GLP-1R/GLP-1 Receptor antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

GLP1R/GLP-1R/GLP-1 Receptor, Polyclonal Antibody (Cat# AAA12337)

Full Name

Rabbit Polyclonal to Human GLP1R/GLP-1R/GLP-1 Receptor

Reactivity

Gibbon, Gorilla, Human
Predicted Reactivity: Monkey, Mouse, Rat, Bovine, Hamster, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin)

Purity

Immunoaffinity Purified

Pricing

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IHC (Immunohistochemistry) (ARL6 Antibody-Immunohistochemistry of paraffin-embedded human colon using ARL6 antibody at dilution of 1:100 (x40 lens).)

ARL6, Polyclonal Antibody (Cat# AAA21297)

Full Name

ARL6 Rabbit anti-Human Polyclonal Antibody

Gene Names

ARL6; BBS3; RP55

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of 293T cells usingug p53 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using p53 Polyclonal Antibody at a dilution of 1:3000.)

p53, Polyclonal Antibody (Cat# AAA22321)

Full Name

(KO Validated) p53 Polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SH-SY5Y cells using anti-SBK1 antibody (AAA20008).Overlay histogram showing SH-SY5Y cells stained with AAA20008 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SBK1 Antibody (AAA20008, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of SBK1 using anti-SBK1 antibody (AAA20008).SBK1 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat C6 whole cell lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse Neuro-2a whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SBK1 antigen affinity purified polyclonal antibody (#AAA20008) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SBK1 at approximately 43 kDa. The expected band size for SBK1 is at 46 kDa.)

SBK1, Polyclonal Antibody (Cat# AAA20008)

Full Name

Anti-SBK1 Antibody Picoband

Gene Names

SBK1; SBK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-P4HA2 AntibodyTitration: 1.0 ug/mlPositive Control: HepG2 Whole Cell)

P4HA2, Polyclonal Antibody (Cat# AAA23592)

Full Name

P4HA2 antibody - C-terminal region

Gene Names

P4HA2; MYP25

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-IKZF3 antibody (AAA19753).Overlay histogram showing THP-1 cells stained with AAA19753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKZF3 Antibody (AAA19753, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of IKZF3 using anti-IKZF3 antibody (AAA19753) and anti-Beta Tubulin antibody (M01857-3).IKZF3 was detected in immunocytochemical section of SIHA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IKZF3 Antibody (AAA19753) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IKZF3 Antibody (AAA19753) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IKZF3 Antibody (AAA19753) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Ramos whole cell lysates,Lane 3: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF3 antigen affinity purified polyclonal antibody (#AAA19753) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IKZF3 at approximately 70 kDa. The expected band size for IKZF3 is at 58 kDa.)

IKZF3, Polyclonal Antibody (Cat# AAA19753)

Full Name

Anti-IKZF3 Antibody Picoband

Gene Names

IKZF3; AIO; AIOLOS; ZNFN1A3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

IHC (Immunohistchemistry) (Figure 9. IHC analysis of SMIM8 using anti-SMIM8 antibody (AAA20009).SMIM8 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

SMIM8, Polyclonal Antibody (Cat# AAA20009)

Full Name

Anti-SMIM8 Antibody

Gene Names

SMIM8; C6orf162; dJ102H19.2

Reactivity

Human

Applications

Immunohistochemistry (IHC), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-GNA11 AntibodyTitration: 1.0 ug/mlPositive Control: Fetal Brain)

GNA11, Polyclonal Antibody (Cat# AAA23594)

Full Name

GNA11 antibody - C-terminal region

Gene Names

GNA11; FBH; FBH2; FHH2; HHC2; GNA-11; HYPOC2

Reactivity

Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Bcl-x, Polyclonal Antibody (Cat# AAA28970)

Full Name

Bcl-x Polyclonal Antibody

Gene Names

BCL2L1; BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

p57, Polyclonal Antibody (Cat# AAA29226)

Full Name

p57 Polyclonal Antibody

Gene Names

CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Anti-GALR2 / Galanin Receptor 2 antibody IHC of human skin hair follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

GALR2 / Galanin Receptor 2, Polyclonal Antibody (Cat# AAA12331)

Full Name

Rabbit Polyclonal to Human GALR2 / Galanin Receptor 2

Gene Names

GALR2; GAL2-R; GALNR2; GALR-2

Reactivity

Human
Predicted Reactivity: Gorilla, Gibbon, Monkey, Mouse, Rat, Bovine, Hamster, Horse (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin)

Purity

Immunoaffinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat testis cells using GPX4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

GPX4, Polyclonal Antibody (Cat# AAA22318)

Full Name

(KD Validated) GPX4 Polyclonal Antibody

Gene Names

GPX4; MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Amphiphysin II, Polyclonal Antibody (Cat# AAA29230)

Full Name

Amphiphysin II Polyclonal Antibody

Gene Names

BIN1; AMPH2; AMPHL; SH3P9

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (GPT/Alanine Transaminase Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)

GPT/Alanine Transaminase, Polyclonal Antibody (Cat# AAA21291)

Full Name

GPT/Alanine Transaminase Rabbit anti-Human Polyclonal (aa147-263) Antibody

Gene Names

GPT; AAT1; ALT1; GPT1

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

HRI, Polyclonal Antibody (Cat# AAA22059)

Full Name

EIF2AK1 Polyclonal Antibody

Reactivity

Human, Mouse, Monkey

Applications

Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), ELISA (EIA)

Purity

Affinity purification

Pricing

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Transfection Control (Anti-XPR1 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

XPR1, Polyclonal Antibody (Cat# AAA12332)

Full Name

Rabbit Polyclonal to Human XPR1

Gene Names

XPR1; X3; SYG1

Reactivity

Gibbon, Hamster, Human, Monkey, Mouse, Rabbit
Predicted Reactivity: Rat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)

Purity

Immunoaffinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-SQRDL/SQOR antibody (AAA20012).Overlay histogram showing JK cells stained with AAA20012 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SQRDL/SQOR Antibody (AAA20012, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of SQRDL/SQOR using anti-SQRDL/SQOR antibody (AAA20012).SQRDL/SQOR was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SQRDL/SQOR Antibody (AAA20012) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SQRDL/SQOR Antibody (AAA20012) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SQRDL/SQOR Antibody (AAA20012) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SQRDL/SQOR Antibody (AAA20012) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQRDL/SQOR antigen affinity purified polyclonal antibody (#AAA20012) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SQRDL/SQOR at approximately 50 kDa. The expected band size for SQRDL/SQOR is at 50 kDa.)

SQRDL/SQOR, Polyclonal Antibody (Cat# AAA20012)

Full Name

Anti-SQRDL/SQOR Antibody Picoband

Gene Names

SQOR; SQR; SQRDL; CGI-44; PRO1975

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

TFEB, Polyclonal Antibody (Cat# AAA22060)

Full Name

TFEB Polyclonal Antibody

Gene Names

TFEB; TCFEB; BHLHE35; ALPHATFEB

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western BlotPositive WB detected in:Mouse brain tissue,Mouse skeletal muscle tissue,ZebrafishAll lanes: Bmp4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDa)

Bone morphogenetic protein 4, Polyclonal Antibody (Cat# AAA26924)

Full Name

Rabbit anti-human Bone morphogenetic protein 4 polyclonal Antibody

Gene Names

BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

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Amyloid-beta, Polyclonal Antibody (Cat# AAA30764)

Full Name

Amyloid-beta Rabbit Polyclonal Antibody

Gene Names

APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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FCM (Flow Cytometry) (Figure 15. Flow Cytometry analysis of Raji cells using anti-Sam68/KHDRBS1 antibody (AAA19757).Overlay histogram showing Raji cells stained with AAA19757 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (AAA19757) and anti-Beta Tubulin antibody (M01857-3).Sam68/KHDRBS1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: human Daudi whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sam68/KHDRBS1 antigen affinity purified polyclonal antibody (#AAA19757) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sam68/KHDRBS1 at approximately 68 kDa. The expected band size for Sam68/KHDRBS1 is at 48 kDa.)

Sam68/KHDRBS1, Polyclonal Antibody (Cat# AAA19757)

Full Name

Anti-Sam68/KHDRBS1 Antibody Picoband

Gene Names

KHDRBS1; p62; p68; Sam68

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (FOLR1/Folate Receptor Alpha Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)

FOLR1/Folate Receptor Alpha, Polyclonal Antibody (Cat# AAA21293)

Full Name

FOLR1/Folate Receptor Alpha Rabbit anti-Human Polyclonal (aa25-234) Antibody

Gene Names

FOLR1; FBP; FOLR

Reactivity

Human

Applications

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:100 (40x lens).)

SERPINB1, Polyclonal Antibody (Cat# AAA22061)

Full Name

SERPINB1 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using FXN/Frataxin Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

FXN/Frataxin, Polyclonal Antibody (Cat# AAA22317)

Full Name

FXN/Frataxin Polyclonal Antibody

Gene Names

FXN; FA; X25; CyaY; FARR; FRDA

Reactivity

Human, Mouse

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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PTEN, Polyclonal Antibody (Cat# AAA30765)

Full Name

PTEN Rabbit Polyclonal Antibody

Gene Names

PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IHC (Immunohistchemistry) (Anti-GPER1 / GPR30 antibody IHC of human colon, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

GPER1 / GPR30, Polyclonal Antibody (Cat# AAA12334)

Full Name

Rabbit Polyclonal to Human GPER1 / GPR30

Gene Names

GPER1; mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br

Reactivity

Gorilla, Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin)

Purity

Immunoaffinity Purified

Pricing

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Transfection Control (Anti-GPR161 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

GPR161, Polyclonal Antibody (Cat# AAA12330)

Full Name

Rabbit Polyclonal to Human GPR161

Gene Names

GPR161; RE2

Reactivity

Gibbon, Gorilla, Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)

Purity

Immunoaffinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of RT4 cells using anti-PTRHD1 antibody (AAA20010).Overlay histogram showing RT4 cells stained with AAA20010 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTRHD1 Antibody (AAA20010, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PTRHD1 using anti-PTRHD1 antibody (AAA20010) and anti-Beta Tubulin antibody (M01857-3).PTRHD1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTRHD1 Antibody (AAA20010) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTRHD1 Antibody (AAA20010) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTRHD1 Antibody (AAA20010) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTRHD1 Antibody (AAA20010) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293Tt whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTRHD1 antigen affinity purified polyclonal antibody (#AAA20010) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTRHD1 at approximately 16 kDa. The expected band size for PTRHD1 is at 16 kDa.)

PTRHD1, Polyclonal Antibody (Cat# AAA20010)

Full Name

Anti-PTRHD1 Antibody Picoband

Gene Names

PTRHD1; C2orf79

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (MX1/MX Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)

MX1/MX, Polyclonal Antibody (Cat# AAA21290)

Full Name

MX1/MX Rabbit anti-Human Polyclonal (aa80-342) Antibody

Reactivity

Mouse, Human

Applications

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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WB (Western Blot) (Host: RabbitTarget Name: FAM108A1Sample Type: Human JurkatAntibody Dilution: 1.0ug/mlABHD17A is supported by BioGPS gene expression data to be expressed in Jurkat)

FAM108A1, Polyclonal Antibody (Cat# AAA23599)

Full Name

FAM108A1 Antibody - C-terminal region

Gene Names

ABHD17A; C19orf27; FAM108A1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

HPA1, Polyclonal Antibody (Cat# AAA29231)

Full Name

HPA1 Polyclonal Antibody

Gene Names

HPSE; HPA; HPA1; HPR1; HSE1; HPSE1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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