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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HEL cells using anti-AHRR antibody (AAA19751).Overlay histogram showing HEL cells stained with AAA19751 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHRR Antibody (AAA19751, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of AHRR using anti-AHRR antibody (AAA19751) and anti-Beta Tubulin antibody (M01857-3).AHRR was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human SiHa whole cell lysates,Lane 4: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHRR antigen affinity purified polyclonal antibody (#AAA19751) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AHRR at approximately 100 kDa. The expected band size for AHRR is at 76 kDa.)

AHRR, Polyclonal Antibody (Cat# AAA19751)

Full Name

Anti-AHRR Antibody Picoband

Gene Names

AHRR; AHH; AHHR; bHLHe77

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using GTF2I antibody (40x lens).)

GTF2I, Polyclonal Antibody (Cat# AAA28199)

Full Name

GTF2I Polyclonal Antibody

Gene Names

GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western blot analysis of lysate from mouse lung tissue lysate, using Mlkl Antibody (C-term). AAA28711 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

M Mlkl, Polyclonal Antibody (Cat# AAA28711)

Full Name

M Mlkl Antibody (C-term)

Gene Names

Mlkl; 9130019I15Rik

Reactivity

Mouse

Applications

Western Blot (WB), ELISA (EIA)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.)

AP-1, Polyclonal Antibody (Cat# AAA28967)

Full Name

AP-1 Polyclonal Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunoprecipitation (IP)

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

KLF13, Polyclonal Antibody (Cat# AAA29223)

Full Name

KLF13 Polyclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Western Blot analysis of K562 cells using CD1D Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)

CD1D, Polyclonal Antibody (Cat# AAA30759)

Full Name

CD1D Rabbit Polyclonal Antibody

Gene Names

CD1D; R3; CD1A

Reactivity

Human, Rat, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

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IF (Immunofluorescence) (Figure 7 Immunofluorescence Validation of PEX3 in Rat testisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

PEX3, Polyclonal Antibody (Cat# AAA11048)

Full Name

PEX3 (IN) Antibody

Gene Names

PEX3; TRG18; PBD10A

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

Purity

PEX3 Antibody is affinity chromatography purified via peptide column.

Pricing

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IF (Immunofluorescence) (Figure 6. IF analysis of Phox/NCF1 using anti-Phox/NCF1 antibody (AAA19752).Phox/NCF1 was detected in a paraffin-embedded section of rat lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-Phox/NCF1 Antibody (AAA19752) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of P47 Phox/NCF1 using anti-P47 Phox/NCF1 antibody (AAA19752).P47 Phox/NCF1 was detected in a paraffin-embedded section of mouse lymph tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-P47 Phox/NCF1 Antibody (AAA19752) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-P47 Phox/NCF1 Antibody (AAA19752) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human THP-1 whole cell lysates,Lane 3: human Ramos whole cell lysates,Lane 4: rat spleen tissue lysates,Lane 5: mouse thymus tissue lysates,Lane 6: mouse spleen tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P47 Phox/NCF1 antigen affinity purified polyclonal antibody (#AAA19752) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for P47 Phox/NCF1 at approximately 40-50 kDa. The expected band size for P47 Phox/NCF1 is at 45 kDa.)

p47 phox/NCF1, Polyclonal Antibody (Cat# AAA19752)

Full Name

Anti-p47 phox/NCF1 Antibody Picoband

Gene Names

NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SH-SY5Y cells using anti-SBK1 antibody (AAA20008).Overlay histogram showing SH-SY5Y cells stained with AAA20008 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SBK1 Antibody (AAA20008, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of SBK1 using anti-SBK1 antibody (AAA20008).SBK1 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SBK1 Antibody (AAA20008) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat C6 whole cell lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse Neuro-2a whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SBK1 antigen affinity purified polyclonal antibody (#AAA20008) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SBK1 at approximately 43 kDa. The expected band size for SBK1 is at 46 kDa.)

SBK1, Polyclonal Antibody (Cat# AAA20008)

Full Name

Anti-SBK1 Antibody Picoband

Gene Names

SBK1; SBK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western BlotPositive WB detected in:Mouse brain tissue,Mouse skeletal muscle tissue,ZebrafishAll lanes: Bmp4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDa)

Bone morphogenetic protein 4, Polyclonal Antibody (Cat# AAA26924)

Full Name

Rabbit anti-human Bone morphogenetic protein 4 polyclonal Antibody

Gene Names

BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

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Amyloid-beta, Polyclonal Antibody (Cat# AAA30764)

Full Name

Amyloid-beta Rabbit Polyclonal Antibody

Gene Names

APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

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FCM (Flow Cytometry) (Figure 15. Flow Cytometry analysis of Raji cells using anti-Sam68/KHDRBS1 antibody (AAA19757).Overlay histogram showing Raji cells stained with AAA19757 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (AAA19757) and anti-Beta Tubulin antibody (M01857-3).Sam68/KHDRBS1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: human Daudi whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sam68/KHDRBS1 antigen affinity purified polyclonal antibody (#AAA19757) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sam68/KHDRBS1 at approximately 68 kDa. The expected band size for Sam68/KHDRBS1 is at 48 kDa.)

Sam68/KHDRBS1, Polyclonal Antibody (Cat# AAA19757)

Full Name

Anti-Sam68/KHDRBS1 Antibody Picoband

Gene Names

KHDRBS1; p62; p68; Sam68

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (FOLR1/Folate Receptor Alpha Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)

FOLR1/Folate Receptor Alpha, Polyclonal Antibody (Cat# AAA21293)

Full Name

FOLR1/Folate Receptor Alpha Rabbit anti-Human Polyclonal (aa25-234) Antibody

Gene Names

FOLR1; FBP; FOLR

Reactivity

Human

Applications

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:100 (40x lens).)

SERPINB1, Polyclonal Antibody (Cat# AAA22061)

Full Name

SERPINB1 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using FXN/Frataxin Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

FXN/Frataxin, Polyclonal Antibody (Cat# AAA22317)

Full Name

FXN/Frataxin Polyclonal Antibody

Gene Names

FXN; FA; X25; CyaY; FARR; FRDA

Reactivity

Human, Mouse

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-P4HA2 AntibodyTitration: 1.0 ug/mlPositive Control: HepG2 Whole Cell)

P4HA2, Polyclonal Antibody (Cat# AAA23592)

Full Name

P4HA2 antibody - C-terminal region

Gene Names

P4HA2; MYP25

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-IKZF3 antibody (AAA19753).Overlay histogram showing THP-1 cells stained with AAA19753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKZF3 Antibody (AAA19753, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of IKZF3 using anti-IKZF3 antibody (AAA19753) and anti-Beta Tubulin antibody (M01857-3).IKZF3 was detected in immunocytochemical section of SIHA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IKZF3 Antibody (AAA19753) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IKZF3 Antibody (AAA19753) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IKZF3 Antibody (AAA19753) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Ramos whole cell lysates,Lane 3: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF3 antigen affinity purified polyclonal antibody (#AAA19753) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IKZF3 at approximately 70 kDa. The expected band size for IKZF3 is at 58 kDa.)

IKZF3, Polyclonal Antibody (Cat# AAA19753)

Full Name

Anti-IKZF3 Antibody Picoband

Gene Names

IKZF3; AIO; AIOLOS; ZNFN1A3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

IHC (Immunohistchemistry) (Figure 9. IHC analysis of SMIM8 using anti-SMIM8 antibody (AAA20009).SMIM8 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

SMIM8, Polyclonal Antibody (Cat# AAA20009)

Full Name

Anti-SMIM8 Antibody

Gene Names

SMIM8; C6orf162; dJ102H19.2

Reactivity

Human

Applications

Immunohistochemistry (IHC), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (GPT/Alanine Transaminase Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)

GPT/Alanine Transaminase, Polyclonal Antibody (Cat# AAA21291)

Full Name

GPT/Alanine Transaminase Rabbit anti-Human Polyclonal (aa147-263) Antibody

Gene Names

GPT; AAT1; ALT1; GPT1

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

HRI, Polyclonal Antibody (Cat# AAA22059)

Full Name

EIF2AK1 Polyclonal Antibody

Reactivity

Human, Mouse, Monkey

Applications

Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), ELISA (EIA)

Purity

Affinity purification

Pricing

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Transfection Control (Anti-XPR1 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

XPR1, Polyclonal Antibody (Cat# AAA12332)

Full Name

Rabbit Polyclonal to Human XPR1

Gene Names

XPR1; X3; SYG1

Reactivity

Gibbon, Hamster, Human, Monkey, Mouse, Rabbit
Predicted Reactivity: Rat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)

Purity

Immunoaffinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-SQRDL/SQOR antibody (AAA20012).Overlay histogram showing JK cells stained with AAA20012 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SQRDL/SQOR Antibody (AAA20012, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of SQRDL/SQOR using anti-SQRDL/SQOR antibody (AAA20012).SQRDL/SQOR was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SQRDL/SQOR Antibody (AAA20012) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SQRDL/SQOR Antibody (AAA20012) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SQRDL/SQOR Antibody (AAA20012) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SQRDL/SQOR Antibody (AAA20012) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQRDL/SQOR antigen affinity purified polyclonal antibody (#AAA20012) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SQRDL/SQOR at approximately 50 kDa. The expected band size for SQRDL/SQOR is at 50 kDa.)

SQRDL/SQOR, Polyclonal Antibody (Cat# AAA20012)

Full Name

Anti-SQRDL/SQOR Antibody Picoband

Gene Names

SQOR; SQR; SQRDL; CGI-44; PRO1975

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

TFEB, Polyclonal Antibody (Cat# AAA22060)

Full Name

TFEB Polyclonal Antibody

Gene Names

TFEB; TCFEB; BHLHE35; ALPHATFEB

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-ADRM1 antibodyTitration: 1 ug/mlPositive Control: HeLa cells and cytosol from male mouse liver)

ADRM1, Polyclonal Antibody (Cat# AAA23588)

Full Name

ADRM1 antibody - C-terminal region

Gene Names

ADRM1; ARM1; ARM-1; GP110

Reactivity

Guinea Pig, Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U937 cells using anti-TMEM132B antibody (AAA20006).Overlay histogram showing U937 cells stained with AAA20006 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM132B Antibody (AAA20006, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of TMEM132B using anti-TMEM132B antibody (AAA20006).TMEM132B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM132B Antibody (AAA20006) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM132B Antibody (AAA20006) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM132B Antibody (AAA20006) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM132B antigen affinity purified polyclonal antibody (#AAA20006) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TMEM132B at approximately 95 kDa. The expected band size for TMEM132B is at 95 kDa.)

TMEM132B, Polyclonal Antibody (Cat# AAA20006)

Full Name

Anti-TMEM132B Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Keratin-pan, Polyclonal Antibody (Cat# AAA29222)

Full Name

Keratin-pan Polyclonal Antibody

Gene Names

KRT2; K2e; KRTE; CK-2e; KRT2A; KRT2E

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Western Blot analysis of HepG2 cells using SPTLC1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)

SPTLC1, Polyclonal Antibody (Cat# AAA30758)

Full Name

SPTLC1 Rabbit Polyclonal Antibody

Gene Names

SPTLC1; HSN1; LBC1; LCB1; SPT1; SPTI; HSAN1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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WB (Western Blot) (WB Suggested Anti-GNA11 AntibodyTitration: 1.0 ug/mlPositive Control: Fetal Brain)

GNA11, Polyclonal Antibody (Cat# AAA23594)

Full Name

GNA11 antibody - C-terminal region

Gene Names

GNA11; FBH; FBH2; FHH2; HHC2; GNA-11; HYPOC2

Reactivity

Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Bcl-x, Polyclonal Antibody (Cat# AAA28970)

Full Name

Bcl-x Polyclonal Antibody

Gene Names

BCL2L1; BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

p57, Polyclonal Antibody (Cat# AAA29226)

Full Name

p57 Polyclonal Antibody

Gene Names

CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Anti-GALR2 / Galanin Receptor 2 antibody IHC of human skin hair follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

GALR2 / Galanin Receptor 2, Polyclonal Antibody (Cat# AAA12331)

Full Name

Rabbit Polyclonal to Human GALR2 / Galanin Receptor 2

Gene Names

GALR2; GAL2-R; GALNR2; GALR-2

Reactivity

Human
Predicted Reactivity: Gorilla, Gibbon, Monkey, Mouse, Rat, Bovine, Hamster, Horse (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin)

Purity

Immunoaffinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence - anti-mScarlet Ab using hCEC cells transduced with mScarlet-Rab5a; cells were fixed with methanol and anti-mScarlet at 1/250;)

mScarlet, Polyclonal Antibody (Cat# AAA13885)

Full Name

anti-mScarlet

Reactivity

Reacts with Transfected cells proteins

Applications

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunoelectron Microscopy (IEM)

Purity

This antibody is epitope-affinity purified from goat antiserum.

Pricing

Goat anti Rabbit IgG, Polyclonal Secondary Antibody (Cat# AAA14909)

Full Name

Goat Anti-Rabbit IgG-UNLB

Reactivity

Rabbit

Applications

ELISA; FLISA; Immunoblot; Immunofluorescence; Immunohistochemistry

Purity

Affinity chromatography on rabbit IgG covalently linked to agarose

Pricing

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of K562 cells using anti-ITPA antibody (AAA19773).Overlay histogram showing K562 cells stained with AAA19773 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ITPA Antibody (AAA19773, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of ITPA using anti-ITPA antibody (AAA19773).ITPA was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ITPA Antibody (AAA19773) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPA antigen affinity purified polyclonal antibody (#AAA19773) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ITPA at approximately 21 kDa. The expected band size for ITPA is at 21 kDa.)

ITPA, Polyclonal Antibody (Cat# AAA19773)

Full Name

Anti-ITPA Antibody Picoband

Gene Names

ITPA; My049; C20orf37; dJ794I6.3; HLC14-06-P

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Cdc2, Polyclonal Antibody (Cat# AAA28989)

Full Name

Cdc2 Polyclonal Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

RANKL, Polyclonal Antibody (Cat# AAA29245)

Full Name

RANKL Polyclonal Antibody

Gene Names

TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using CHRFAM7A antibody at dilution of 1:100 (40x lens).)

CHRFAM7A, Polyclonal Antibody (Cat# AAA28208)

Full Name

CHRFAM7A Polyclonal Antibody

Gene Names

CHRFAM7A; D-10; CHRNA7; CHRNA7-DR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.)

Caspase-7, Polyclonal Antibody (Cat# AAA28976)

Full Name

Caspase-7 Polyclonal Antibody

Gene Names

CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

MMP-9, Polyclonal Antibody (Cat# AAA29232)

Full Name

MMP-9 Polyclonal Antibody

Gene Names

MMP9; GELB; CLG4B; MMP-9; MANDP2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:200)

Napsin A, Polyclonal Antibody (Cat# AAA30768)

Full Name

Napsin A Rabbit Polyclonal Antibody

Gene Names

NAPSA; KAP; Kdap; NAP1; NAPA; SNAPA

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IHC (Immunohistchemistry) (Anti-GLP1R/GLP-1R/GLP-1 Receptor antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

GLP1R/GLP-1R/GLP-1 Receptor, Polyclonal Antibody (Cat# AAA12337)

Full Name

Rabbit Polyclonal to Human GLP1R/GLP-1R/GLP-1 Receptor

Reactivity

Gibbon, Gorilla, Human
Predicted Reactivity: Monkey, Mouse, Rat, Bovine, Hamster, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin)

Purity

Immunoaffinity Purified

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (ARL6 Antibody-Immunohistochemistry of paraffin-embedded human colon using ARL6 antibody at dilution of 1:100 (x40 lens).)

ARL6, Polyclonal Antibody (Cat# AAA21297)

Full Name

ARL6 Rabbit anti-Human Polyclonal Antibody

Gene Names

ARL6; BBS3; RP55

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of 293T cells usingug p53 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using p53 Polyclonal Antibody at a dilution of 1:3000.)

p53, Polyclonal Antibody (Cat# AAA22321)

Full Name

(KO Validated) p53 Polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

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<< Previous Next >>

WB (Western Blot) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

PACSIN1, Polyclonal Antibody (Cat# AAA28726)

Full Name

PACSIN1 Antibody (N-term)

Gene Names

PACSIN1; SDPI

Reactivity

Human, mouse, rat (Predicted Reactivity: Rat, Chicken, Bovine, Xenopus)

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Caco-2 cells using anti-MATK antibody (AAA19767).Overlay histogram showing Caco-2 cells stained with AAA19767 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MATK Antibody (AAA19767, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of MATK using anti-MATK antibody (AAA19767).MATK was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse thymus tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATK antigen affinity purified polyclonal antibody (#AAA19767) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MATK at approximately 52 kDa. The expected band size for MATK is at 56 kDa.)

MATK, Polyclonal Antibody (Cat# AAA19767)

Full Name

Anti-MATK Antibody Picoband

Gene Names

MATK; CHK; CTK; HYL; Lsk; HYLTK; HHYLTK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

TOM20, Polyclonal Antibody (Cat# AAA22327)

Full Name

(KD Validated) TOM20 Polyclonal Antibody

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Host: RabbitTarget Name: GBRPSample Type: Jurkat Whole Cell lysatesAntibody Dilution: 1ug/ml)

GABRP, Polyclonal Antibody (Cat# AAA23607)

Full Name

GABRP antibody - N-terminal region

Reactivity

Dog, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Anti-tdTomato Ab conjugated to DyLight 550 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane;)

tdTomato, Polyclonal Antibody (Cat# AAA13880)

Full Name

Anti-tdTomato, DyLight550

Reactivity

Reacts with Transfected cells proteins

Applications

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Frozen (IHC-F)

Purity

This antibody is epitope-affinity purified from goat antiserum.

Pricing

Goat Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14904)

Full Name

Goat Anti-Human IgG-HRP

Applications

ELISA

Purity

Affinity chromatography on human IgG covalently linked to agarose

Pricing

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FCM (Flow Cytometry) (Figure 5. Flow Cytometry analysis of HepG2 cells using anti-PGM3 antibody (AAA19768).Overlay histogram showing HepG2 cells stained with AAA19768 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGM3 Antibody (AAA19768, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 4. IF analysis of PGM3 using anti-PGM3 antibody (AAA19768) and anti-Beta Tubulin antibody (M01857-3).PGM3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGM3 Antibody (AAA19768) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGM3 Antibody (AAA19768) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human k562 whole cell lysates,Lane 5: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGM3 antigen affinity purified polyclonal antibody (#AAA19768) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PGM3 at approximately 63 kDa. The expected band size for PGM3 is at 60 kDa.)

PGM3, Polyclonal Antibody (Cat# AAA19768)

Full Name

Anti-PGM3 Antibody Picoband

Gene Names

PGM3; AGM1; PAGM; IMD23; PGM 3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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