Polyclonal Antibodies

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IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 using CTGF Polyclonal Antibody at dilution of 1 : 150 (40x lens). Blue: DAPI for nuclear staining.)

CTGF, Polyclonal Antibody (Cat# AAA22293)

Full Name

CTGF Polyclonal Antibody

Gene Names

CTGF; CCN2; NOV2; HCS24; IGFBP8

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-CRBN Antibody Titration: 0.2-1 ug/mlPositive Control: COLO205 cell lysate)

CRBN, Polyclonal Antibody (Cat# AAA23573)

Full Name

CRBN antibody - N-terminal region

Gene Names

CRBN; MRT2; MRT2A

Reactivity

Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26901)

Full Name

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Gene Names

PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha

Reactivity

Human

Applications

Immunocytochemistry (ICC), FLISA, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Purity

Purified by Immunoaffinity chromatography.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using DOK1 Antibody at dilution of 1:100 (40x lens).)

DOK1, Polyclonal Antibody (Cat# AAA28181)

Full Name

DOK1 Polyclonal Antibody

Gene Names

DOK1; P62DOK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

MUC1, Polyclonal Antibody (Cat# AAA30739)

Full Name

MUC1 Antibody

Gene Names

MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-p, IF (paraffin-section), ELISA

Purity

The antibody was affinity-purified from rabbit antiserum by affinity- chromatography using epitope-specific immunogen.

Pricing

JAK1, Polyclonal Antibody (Cat# AAA28692)

Full Name

JAK1 Antibody (C-term)

Gene Names

JAK1; JTK3; JAK1A; JAK1B

Reactivity

Human, mouse

Applications

Western Blot (WB), ELISA (EIA)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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ACVR2A, Polyclonal Antibody (Cat# AAA10774)

Full Name

ACVR2A Polyclonal Antibody

Gene Names

ACVR2A; ACVR2; ACTRII

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using POP4 antibody at dilution of 1:100 (40x lens).)

POP4, Polyclonal Antibody (Cat# AAA28182)

Full Name

POP4 Polyclonal Antibody

Gene Names

POP4; RPP29

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

CDCP1, Polyclonal Antibody (Cat# AAA29206)

Full Name

CDCP1 Polyclonal Antibody

Gene Names

CDCP1; CD318; TRASK; SIMA135

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using CBFB antibody. Blue: DAPI for nuclear staining.)

CBFB, Polyclonal Antibody (Cat# AAA28178)

Full Name

CBFB Polyclonal Antibody

Gene Names

CBFB; PEBP2B

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using LEO1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

LEO1, Polyclonal Antibody (Cat# AAA22291)

Full Name

LEO1 Polyclonal Antibody

Gene Names

LEO1; RDL

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26899)

Full Name

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Gene Names

PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha

Reactivity

Human

Applications

Immunocytochemistry (ICC), FLISA, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Purity

Purified by Immunoaffinity chromatography.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of Jurkat cells.)

ETS1/ETS-1, Polyclonal Antibody (Cat# AAA21275)

Full Name

Anti-ETS1/ETS-1 Antibody

Gene Names

ETS1; p54; ETS-1; EWSR2; c-ets-1

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of A549 cells using anti-SHKBP1 antibody (AAA19991).Overlay histogram showing A549 cells stained with AAA19991 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHKBP1 Antibody (AAA19991, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of SHKBP1 using anti-SHKBP1 antibody (AAA19991).SHKBP1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates,Lane 3: human U251 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHKBP1 antigen affinity purified polyclonal antibody (#AAA19991) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SHKBP1 at approximately 76 kDa. The expected band size for SHKBP1 is at 76 kDa.)

SHKBP1, Polyclonal Antibody (Cat# AAA19991)

Full Name

Anti-SHKBP1 Antibody Picoband

Gene Names

SHKBP1; Sb1; PP203

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using CAMK1D antibody.)

CAMK1D, Polyclonal Antibody (Cat# AAA28183)

Full Name

CAMK1D

Gene Names

CAMK1D; CKLiK; CaM-K1; CaMKID

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

VGF, Polyclonal Antibody (Cat# AAA29207)

Full Name

VGF Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using MYO1C antibody. Blue: DAPI for nuclear staining.)

MYO1C, Polyclonal Antibody (Cat# AAA10776)

Full Name

MYO1C Polyclonal Antibody

Gene Names

MYO1C; NMI; MMIb; myr2; MMI-beta

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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Nono, Polyclonal Antibody (Cat# AAA28188)

Full Name

Nono Polyclonal Antibody

Gene Names

NONO; P54; NMT55; NRB54; P54NRB; PPP1R114

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), RIP

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells using [KD Validated] COX IV Rabbit pAb (AAA28444) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

COX IV, Polyclonal Antibody (Cat# AAA28444)

Full Name

[KD Validated] COX IV Rabbit pAb

Gene Names

COX4I1; COX4; COXIV; COX4-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

MOR-1, Polyclonal Antibody (Cat# AAA28956)

Full Name

MOR-1 (phospho Ser375) Polyclonal Antibody

Gene Names

OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Lymphotactin, Polyclonal Antibody (Cat# AAA29212)

Full Name

Lymphotactin Polyclonal Antibody

Gene Names

XCL1; LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RNF2 antibody. Western blot was performed from the immunoprecipitate using RNF2 antibody at a dilition of 1:1000.)

RNF2, Polyclonal Antibody (Cat# AAA10781)

Full Name

RNF2 Polyclonal Antibody

Gene Names

RNF2; BAP1; DING; BAP-1; HIPI3; RING2; RING1B

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-APLP2 antibody (AAA19746).Overlay histogram showing HepG2 cells stained with AAA19746 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APLP2 Antibody (AAA19746, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of APLP2 using anti-APLP2 antibody (AAA19746).APLP2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-APLP2 Antibody (AAA19746) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APLP2 antigen affinity purified polyclonal antibody (#AAA19746) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APLP2 at approximately 100-110 kDa. The expected band size for APLP2 is at 87 kDa.)

APLP2, Polyclonal Antibody (Cat# AAA19746)

Full Name

Anti-APLP2 Antibody Picoband

Gene Names

APLP2; APPH; APPL2; CDEBP; APLP-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U20S cells using anti-LRRN4 antibody (AAA20002).Overlay histogram showing U20S cells stained with AAA20002 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LRRN4 Antibody (AAA20002, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of LRRN4 using anti-LRRN4 antibody (AAA20002).LRRN4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U20S whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRN4 antigen affinity purified polyclonal antibody (#AAA20002) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRN4 at approximately 79 kDa. The expected band size for LRRN4 is at 79 kDa.)

LRRN4, Polyclonal Antibody (Cat# AAA20002)

Full Name

Anti-LRRN4 Antibody Picoband

Gene Names

LRRN4; NLRR4; NLRR-4; C20orf75; dJ1056H1.1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using [KO Validated] CDK2 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.)

CDK2, Polyclonal Antibody (Cat# AAA22306)

Full Name

(KO Validated) CDK2 Polyclonal Antibody

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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Endoglin, Polyclonal Antibody (Cat# AAA30754)

Full Name

Endoglin Rabbit Polyclonal Antibody

Gene Names

ENG; END; ORW; HHT1; ORW1; CD105

Reactivity

Human, Rat, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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WB (Western Blot) (Western blot of Latexin (arrow) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2))

Latexin / MUM, Polyclonal Antibody (Cat# AAA12323)

Full Name

Rabbit Polyclonal to Human Latexin / MUM

Gene Names

LXN; ECI; TCI

Reactivity

Human

Applications

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunoaffinity Purified

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HeLa cells usingug CDK4 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK4 Polyclonal Antibody at a dilution of 1:1000.)

CDK4, Polyclonal Antibody (Cat# AAA22305)

Full Name

(KO Validated) CDK4 Polyclonal Antibody

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-PSEN1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)

PSEN1, Polyclonal Antibody (Cat# AAA23585)

Full Name

PSEN1 antibody - middle region

Gene Names

PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CETN3 antibody at dilution of 1:100 (40x lens).)

CETN3, Polyclonal Antibody (Cat# AAA28193)

Full Name

CETN3 Polyclonal Antibody

Gene Names

CETN3; CEN3; CDC31

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Actinin-alpha1/2/3/4, Polyclonal Antibody (Cat# AAA28961)

Full Name

Actinin-alpha1/2/3/4 Polyclonal Antibody

Gene Names

ACTN1; BDPLT15

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Amphiphysin II, Polyclonal Antibody (Cat# AAA29217)

Full Name

Amphiphysin II Polyclonal Antibody

Gene Names

BIN1; AMPH2; AMPHL; SH3P9

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of JK cells using anti-TDP-43/TARDBP antibody (AAA19743).Overlay histogram showing JK cells stained with AAA19743 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TDP-43/TARDBP Antibody (AAA19743, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of TDP-43/TARDBP using anti-TDP-43/TARDBP antibody (AAA19743) and anti-Beta Tubulin antibody (M01857-3).TDP-43/TARDBP was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse pancrease lysates,Lane 6: mouse Neuro-2a whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDP-43/TARDBP antigen affinity purified polyclonal antibody (#AAA19743) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TDP-43/TARDBP at approximately 43 kDa. The expected band size for TDP-43/TARDBP is at 45 kDa.)

TDP-43/TARDBP, Polyclonal Antibody (Cat# AAA19743)

Full Name

Anti-TDP-43/TARDBP Antibody Picoband

Gene Names

TARDBP; ALS10; TDP-43

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cells.)

POLK/DNA Polymerase Kappa, Polyclonal Antibody (Cat# AAA21279)

Full Name

Anti-POLK/DNA Polymerase Kappa Antibody

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-SOD2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta)

SOD2, Polyclonal Antibody (Cat# AAA23583)

Full Name

SOD2 antibody - N-terminal region

Gene Names

SOD2; IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

14-3-3 sigma, Polyclonal Antibody (Cat# AAA28959)

Full Name

14-3-3 sigma Polyclonal Antibody

Gene Names

SFN; YWHAS

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

CD38, Polyclonal Antibody (Cat# AAA28702)

Full Name

CD38 Antibody (C-term)

Gene Names

CD38; T10; ADPRC 1

Reactivity

Human (Predicted Reactivity: Monkey)

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

ERK 1/2, Polyclonal Antibody (Cat# AAA28958)

Full Name

ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CCT4 antibody. Blue: DAPI for nuclear staining.)

CCT4, Polyclonal Antibody (Cat# AAA10783)

Full Name

CCT4 Polyclonal Antibody

Gene Names

CCT4; SRB; Cctd; CCT-DELTA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-LZIC antibody (AAA20000).Overlay histogram showing 293T cells stained with AAA20000 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LZIC Antibody (AAA20000, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of LZIC using anti-LZIC antibody (AAA20000) and anti-Beta Tubulin antibody (M01857-3).LZIC was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LZIC Antibody (AAA20000) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LZIC Antibody (AAA20000) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LZIC Antibody (AAA20000) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: human HEL whole cell lysates,Lane 6: human Jurkat whole cell lysates,Lane 7: rat brain tissue lysates,Lane 8: rat thymus tissue lysates,Lane 9: mouse brain tissue lysates,Lane 10: mouse thymus tissue lysates,Lane 11: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LZIC antigen affinity purified polyclonal antibody (#AAA20000) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LZIC at approximately 21 kDa. The expected band size for LZIC is at 21 kDa.)

LZIC, Polyclonal Antibody (Cat# AAA20000)

Full Name

Anti-LZIC Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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Prothrombin, Polyclonal Antibody (Cat# AAA30752)

Full Name

Prothrombin Rabbit Polyclonal Antibody

Gene Names

F2; PT; THPH1; RPRGL2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using AAAS antibody. Blue: DAPI for nuclear staining.)

AAAS, Polyclonal Antibody (Cat# AAA10785)

Full Name

AAAS Polyclonal Antibody

Gene Names

AAAS; AAA; AAASb; GL003; ALADIN; ADRACALA; ADRACALIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-METTL18 antibody (AAA20001).Overlay histogram showing A431 cells stained with AAA20001 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL18 Antibody (AAA20001, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of METTL18 using anti-METTL18 antibody (AAA20001) and anti-Beta Tubulin antibody (M01857-3).METTL18 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METTL18 Antibody (AAA20001) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL18 antigen affinity purified polyclonal antibody (#AAA20001) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for METTL18 at approximately 50 kDa. The expected band size for METTL18 is at 42 kDa.)

METTL18, Polyclonal Antibody (Cat# AAA20001)

Full Name

Anti-METTL18 Antibody Picoband

Gene Names

METTL18; HPM1; AsTP2; C1orf156

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

EPAS-1, Polyclonal Antibody (Cat# AAA29219)

Full Name

EPAS-1 Polyclonal Antibody

Gene Names

EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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EGF, Polyclonal Antibody (Cat# AAA30755)

Full Name

EGF Rabbit Polyclonal Antibody

Gene Names

EGF; URG; HOMG4

Reactivity

Human, Rat, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IHC (Immunohistochemistry) (Figure 7: Immunohistochemistry Validation of AIFM2 in Rat TestisImmunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AIFM2 antibody (AAA11044) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

AIFM2, Polyclonal Antibody (Cat# AAA11044)

Full Name

AIFM2 (CT) Antibody

Gene Names

AIFM2; AMID; PRG3; RP11-367H5.2

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

AIMF2 Antibody is affinity chromatography purified via peptide column.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-RARS/RARS1 antibody (AAA19748).Overlay histogram showing MCF-7 cells stained with AAA19748 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RARS/RARS1 Antibody (AAA19748, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of RARS/RARS1 using anti-RARS/RARS1 antibody (AAA19748) and anti-Tubulin Alpha antibody (M03989-3).RARS/RARS1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RARS/RARS1 Antibody (AAA19748) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RARS/RARS1 Antibody (AAA19748) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RARS/RARS1 Antibody (AAA19748) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARS/RARS1 antigen affinity purified polyclonal antibody (#AAA19748) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RARS/RARS1 at approximately 70 kDa. The expected band size for RARS/RARS1 is at 75 kDa.)

RARS/RARS1, Polyclonal Antibody (Cat# AAA19748)

Full Name

Anti-RARS/RARS1 Antibody Picoband

Gene Names

RARS; HLD9; ArgRS; DALRD1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 7. IF analysis of MAP6D1 using anti-MAP6D1 antibody (AAA20004).MAP6D1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP6D1 Antibody (AAA20004, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of MAP6D1 using anti-MAP6D1 antibody (AAA20004).MAP6D1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP6D1 Antibody (AAA20004) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP6D1 Antibody (AAA20004) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP6D1 Antibody (AAA20004) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP6D1 antigen affinity purified polyclonal antibody (#AAA20004) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP6D1 at approximately 21 kDa. The expected band size for MAP6D1 is at 21 kDa.)

MAP6D1, Polyclonal Antibody (Cat# AAA20004)

Full Name

Anti-MAP6D1 Antibody Picoband

Gene Names

MAP6D1; SL21; MAPO6D1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Cyclin B1, Polyclonal Antibody (Cat# AAA22301)

Full Name

Cyclin B1 Polyclonal Antibody

Gene Names

CCNB1; CCNB

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using PDYN antibody at dilution of 1:100 (40x lens).)

PDYN, Polyclonal Antibody (Cat# AAA10782)

Full Name

PDYN

Gene Names

PDYN; ADCA; PENKB; SCA23

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Pricing

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Viewing 100-150 of 3252 product results

CTGF, Polyclonal Antibody (Cat# AAA22293)

CRBN, Polyclonal Antibody (Cat# AAA23573)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26901)

DOK1, Polyclonal Antibody (Cat# AAA28181)

MUC1, Polyclonal Antibody (Cat# AAA30739)

JAK1, Polyclonal Antibody (Cat# AAA28692)

ACVR2A, Polyclonal Antibody (Cat# AAA10774)

POP4, Polyclonal Antibody (Cat# AAA28182)

CDCP1, Polyclonal Antibody (Cat# AAA29206)

CBFB, Polyclonal Antibody (Cat# AAA28178)

LEO1, Polyclonal Antibody (Cat# AAA22291)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26899)

ETS1/ETS-1, Polyclonal Antibody (Cat# AAA21275)

SHKBP1, Polyclonal Antibody (Cat# AAA19991)

CAMK1D, Polyclonal Antibody (Cat# AAA28183)

VGF, Polyclonal Antibody (Cat# AAA29207)

MYO1C, Polyclonal Antibody (Cat# AAA10776)

Nono, Polyclonal Antibody (Cat# AAA28188)

COX IV, Polyclonal Antibody (Cat# AAA28444)

MOR-1, Polyclonal Antibody (Cat# AAA28956)

Lymphotactin, Polyclonal Antibody (Cat# AAA29212)

RNF2, Polyclonal Antibody (Cat# AAA10781)

APLP2, Polyclonal Antibody (Cat# AAA19746)

LRRN4, Polyclonal Antibody (Cat# AAA20002)

CDK2, Polyclonal Antibody (Cat# AAA22306)

Endoglin, Polyclonal Antibody (Cat# AAA30754)

Latexin / MUM, Polyclonal Antibody (Cat# AAA12323)

CDK4, Polyclonal Antibody (Cat# AAA22305)

PSEN1, Polyclonal Antibody (Cat# AAA23585)

CETN3, Polyclonal Antibody (Cat# AAA28193)

Actinin-alpha1/2/3/4, Polyclonal Antibody (Cat# AAA28961)

Amphiphysin II, Polyclonal Antibody (Cat# AAA29217)

TDP-43/TARDBP, Polyclonal Antibody (Cat# AAA19743)

POLK/DNA Polymerase Kappa, Polyclonal Antibody (Cat# AAA21279)

SOD2, Polyclonal Antibody (Cat# AAA23583)

14-3-3 sigma, Polyclonal Antibody (Cat# AAA28959)

CD38, Polyclonal Antibody (Cat# AAA28702)

ERK 1/2, Polyclonal Antibody (Cat# AAA28958)

CCT4, Polyclonal Antibody (Cat# AAA10783)

LZIC, Polyclonal Antibody (Cat# AAA20000)

Prothrombin, Polyclonal Antibody (Cat# AAA30752)

AAAS, Polyclonal Antibody (Cat# AAA10785)

METTL18, Polyclonal Antibody (Cat# AAA20001)

EPAS-1, Polyclonal Antibody (Cat# AAA29219)

EGF, Polyclonal Antibody (Cat# AAA30755)

AIFM2, Polyclonal Antibody (Cat# AAA11044)

RARS/RARS1, Polyclonal Antibody (Cat# AAA19748)

MAP6D1, Polyclonal Antibody (Cat# AAA20004)

Cyclin B1, Polyclonal Antibody (Cat# AAA22301)

PDYN, Polyclonal Antibody (Cat# AAA10782)

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