Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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HDAC5, Polyclonal Antibody (Cat# AAA19236)

Full Name

Anti-HDAC5 Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using NCF4 antibody.Blue: DAPI for nuclear staining.)

NCF4, Polyclonal Antibody (Cat# AAA10738)

Full Name

NCF4 Polyclonal Antibody

Gene Names

NCF4; NCF; P40PHOX; SH3PXD4

Reactivity

Human, Mouse, Rat

Applications

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

Cyclin A, Polyclonal Antibody (Cat# AAA29005)

Full Name

Cyclin A Polyclonal Antibody

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC-P, IF

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using PDK4 antibody.)

PDK4, Polyclonal Antibody (Cat# AAA30723)

Full Name

PDK4 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-RANBP2 antibody (AAA19742).Overlay histogram showing MCF-7 cells stained with AAA19742 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RANBP2 Antibody (AAA19742, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of RANBP2 using anti-RANBP2 antibody (AAA19742).RANBP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RANBP2 Antibody (AAA19742) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP2 antigen affinity purified polyclonal antibody (#AAA19742) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RANBP2 at approximately 358 kDa. The expected band size for RANBP2 is at 358 kDa.)

RANBP2, Polyclonal Antibody (Cat# AAA19742)

Full Name

Anti-RANBP2 Antibody Picoband

Gene Names

RANBP2; ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western blot analysis of extracts from HepG2 and 293, using CALR Antibody.)

CALR, Polyclonal Antibody (Cat# AAA31108)

Full Name

CALR Antibody

Gene Names

CALR; RO; CRT; SSA; cC1qR; HEL-S-99n

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC, EIA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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Ubiquitin C-terminal Hydrolase 1 (UCHL1) ck, Polyclonal Antibody (Cat# AAA14227)

Full Name

Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1)

Gene Names

UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5

Reactivity

Bovine, Human, Mouse, Pig, Rat
Expected Reactivity: Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole

Applications

WB, IF

Purity

Total IgY fraction

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

MIP-3alpha, Polyclonal Antibody (Cat# AAA29367)

Full Name

MIP-3alpha Polyclonal Antibody

Gene Names

CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha

Reactivity

Human, Mouse

Applications

IHC-P

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of JK cells using anti-TDP-43/TARDBP antibody (AAA19743).Overlay histogram showing JK cells stained with AAA19743 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TDP-43/TARDBP Antibody (AAA19743, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of TDP-43/TARDBP using anti-TDP-43/TARDBP antibody (AAA19743) and anti-Beta Tubulin antibody (M01857-3).TDP-43/TARDBP was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TDP-43/TARDBP Antibody (AAA19743) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse pancrease lysates,Lane 6: mouse Neuro-2a whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDP-43/TARDBP antigen affinity purified polyclonal antibody (#AAA19743) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TDP-43/TARDBP at approximately 43 kDa. The expected band size for TDP-43/TARDBP is at 45 kDa.)

TDP-43/TARDBP, Polyclonal Antibody (Cat# AAA19743)

Full Name

Anti-TDP-43/TARDBP Antibody Picoband

Gene Names

TARDBP; ALS10; TDP-43

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U20S cells using anti-LRRN4 antibody (AAA20002).Overlay histogram showing U20S cells stained with AAA20002 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LRRN4 Antibody (AAA20002, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of LRRN4 using anti-LRRN4 antibody (AAA20002).LRRN4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRN4 Antibody (AAA20002) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U20S whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRN4 antigen affinity purified polyclonal antibody (#AAA20002) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRN4 at approximately 79 kDa. The expected band size for LRRN4 is at 79 kDa.)

LRRN4, Polyclonal Antibody (Cat# AAA20002)

Full Name

Anti-LRRN4 Antibody Picoband

Gene Names

LRRN4; NLRR4; NLRR-4; C20orf75; dJ1056H1.1

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

S-100 alpha, Polyclonal Antibody (Cat# AAA29323)

Full Name

S-100 alpha Polyclonal Antibody

Gene Names

S100A1; S100; S100A; S100-alpha

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 antibody at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.)

Acetyl-Histone H4-K12, Polyclonal Antibody (Cat# AAA28313)

Full Name

Acetyl-Histone H4-K12 Rabbit pAb

Gene Names

HIST2H4B; H4/o

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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WB (Western Blot) (Detection of human phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (10 ug) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-phospho KAP-1 (S824) antibody (AAA23773 lot 6) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP1 antibody .)

KAP-1, Polyclonal Antibody (Cat# AAA23773)

Full Name

Rabbit anti-Phospho KAP-1 (S824) Antibody, Affinity Purified

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Reactivity

Human, Mouse

Applications

WB, IP, IHC, ICC, ICC-IF

Purity

Antigen Affinity Purified

Pricing

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WB (Western Blot) (Western blot analysis of HDAC5 expression in mouse brain tissue lysates, The lane on the right is treated with the antigen-specific peptide.)

HDAC5, Polyclonal Antibody (Cat# AAA31097)

Full Name

HDAC5 Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse

Applications

WB, IHC, IF, ICC, EIA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

H7 (H7N9)(A/Shanghai/2/2013), Polyclonal Antibody (Cat# AAA13735)

Full Name

Anti-H7 (H7N9)(A/Shanghai/2/2013)

Applications

WB, EIA, IP

Purity

Immunoaffinity chromatography

Pricing

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IF (Immunofluorescence) (Immunofluorescence of AXIN2 in HepG2 cells with AXIN2 Antibody at 20 ug/ml.)

AXIN2, Polyclonal Antibody (Cat# AAA10910)

Full Name

AXIN2 Antibody

Gene Names

AXIN2; AXIL; ODCRCS

Reactivity

Human, Mouse, Rat

Applications

EIA, WB, IHC-P, IF

Purity

AXIN2 Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples: Mouse Small intestine Tissue; Primary Ab: 30ug/ml Rabbit Anti-Mouse LTBP1 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

Latent Transforming Growth Factor Beta Binding Protein 1, Polyclonal Antibody (Cat# AAA20909)

Full Name

Polyclonal Antibody to Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1)

Gene Names

Ltbp1; Tgfb; Ltbp-1; Ltbp1L; 9830146M04; b2b1000Clo; 9430031G15Rik; TGF-beta1-BP-1

Reactivity

Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of human placenta using ENTPD1/CD39 Polyclonal Antibody.)

ENTPD1/CD39, Polyclonal Antibody (Cat# AAA30668)

Full Name

ENTPD1/CD39 Rabbit Polyclonal Antibody

Gene Names

ENTPD1; CD39; ATPDase; NTPDase-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)

Chemokine C-X3-C-Motif Ligand 1 (CX3CL1), Polyclonal Antibody (Cat# AAA20087)

Full Name

Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)

Gene Names

CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin

Reactivity

Human, Mouse

Applications

ICC, IHC, EIA, WB

Purity

Affinity Chromatography

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Actinin-alpha1/2/3/4, Polyclonal Antibody (Cat# AAA28961)

Full Name

Actinin-alpha1/2/3/4 Polyclonal Antibody

Gene Names

ACTN1; BDPLT15

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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GSK3 beta, Polyclonal Antibody (Cat# AAA31297)

Full Name

Phospho-GSK3 beta (Thr390) Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Bub1, Polyclonal Antibody (Cat# AAA31312)

Full Name

Phospho-Bub1 (Ser459) Antibody

Gene Names

BUB1; BUB1A; BUB1L; hBUB1

Reactivity

Human, Rat

Applications

IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HL-60 cells using anti-PAP/ACP3 antibody (AAA19446).Overlay histogram showing HL-60 cells stained with AAA19446 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAP/ACP3 Antibody (AAA19446, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PAP/ACP3, Polyclonal Antibody (Cat# AAA19446)

Full Name

Anti-PAP/ACP3 Antibody Picoband

Gene Names

ACPP; ACP3; 5'-NT; ACP-3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Cleaved-Caspase-9, Polyclonal Antibody (Cat# AAA28834)

Full Name

Cleaved-Caspase-9 (D353) Polyclonal Antibody

Reactivity

Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

HA (A/California/06/09)(H1N1), Polyclonal Antibody (Cat# AAA13733)

Full Name

Anti-HA (A/California/06/09)(H1N1)

Applications

WB, EIA

Purity

Immunoaffinity chromatography

Pricing

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IHC (Immunohistchemistry) (MGP/Matrix Gla-Protein Antibody-Immunohistochemistry of paraffin-embedded rat brain using MGP antibody at dilution of 1:100 (40x lens).)

MGP/Matrix Gla-Protein, Polyclonal Antibody (Cat# AAA21296)

Full Name

MGP/Matrix Gla-Protein Rabbit anti-Human Polyclonal Antibody

Gene Names

MGP; NTI; GIG36; MGLAP

Reactivity

Mouse, Rat, Human

Applications

IHC, IHC-P, WB

Purity

Affinity purified

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

ERK 1/2, Polyclonal Antibody (Cat# AAA28958)

Full Name

ERK 1/2 (phospho Thr202/Y204) Polyclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Smad1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Smad1, Polyclonal Antibody (Cat# AAA22285)

Full Name

(KO Validated) Smad1 Polyclonal Antibody

Gene Names

SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

Acetyl-Histone H4-K5/K8/K12/K16, Polyclonal Antibody (Cat# AAA28423)

Full Name

Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb

Gene Names

FANCD2; FA4; FAD; FACD; FAD2; FA-D2; FANCD

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U251 cells using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Overlay histogram showing U251 cells stained with AAA19481 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Acid Phosphatase/ACP5 Antibody (AAA19481, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Acid phosphatase/ACP5, Polyclonal Antibody (Cat# AAA19481)

Full Name

Anti-Acid phosphatase/ACP5 Antibody Picoband

Gene Names

ACP5; TRAP; SPENCDI

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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Annexin I, Polyclonal Antibody (Cat# AAA30753)

Full Name

Annexin I Rabbit Polyclonal Antibody

Gene Names

ANXA1; ANX1; LPC1

Reactivity

Human, Rat, Mouse

Applications

IF, ICC, WB, IHC-P, EIA

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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MAPK3, Polyclonal Antibody (Cat# AAA10646)

Full Name

MAPK3 Polyclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Akt, Polyclonal Antibody (Cat# AAA28965)

Full Name

Akt Polyclonal Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Stomach Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse vWF AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

Von Willebrand Factor (vWF), Polyclonal Antibody (Cat# AAA20193)

Full Name

Polyclonal Antibody to Von Willebrand Factor (vWF)

Gene Names

Vwf; VWD; F8VWF; AI551257; C630030D09; 6820430P06Rik; B130011O06Rik

Reactivity

Mouse, Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

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RNASEK, Polyclonal Antibody (Cat# AAA28700)

Full Name

RNASEK Antibody (Center)

Reactivity

Human (Predicted Reactivity: Bovine, Mouse)

Applications

WB, IHC-P, E

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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IHC (Immunohistchemistry) (MX1/MX Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)

MX1/MX, Polyclonal Antibody (Cat# AAA21290)

Full Name

MX1/MX Rabbit anti-Human Polyclonal (aa80-342) Antibody

Reactivity

Mouse, Human

Applications

ICC, IHC, IHC-P, IP, WB

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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Interferon Alpha, Polyclonal Antibody (Cat# AAA21018)

Full Name

Polyclonal Antibody to Interferon Alpha (IFNa)

Gene Names

Ifna1; Ifa1

Reactivity

Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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CCDC59, Polyclonal Antibody (Cat# AAA29808)

Full Name

CCDC59 Polyclonal Antibody

Gene Names

CCDC59; BR22; TAP26; HSPC128

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Histone H4, Polyclonal Antibody (Cat# AAA29039)

Full Name

Histone H4 Polyclonal Antibody

Gene Names

HIST2H4B; H4/o

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC-P, IF

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using MAP2K5 antibody. Blue: DAPI for nuclear staining.)

MAP2K5, Polyclonal Antibody (Cat# AAA28148)

Full Name

MAP2K5 Polyclonal Antibody

Gene Names

MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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RSRC1, Polyclonal Antibody (Cat# AAA10780)

Full Name

RSRC1 Polyclonal Antibody

Gene Names

RSRC1; BM-011; SFRS21; SRrp53

Reactivity

Human

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines.)

PSMA5, Polyclonal Antibody (Cat# AAA21278)

Full Name

Anti-PSMA5 Antibody

Gene Names

PSMA5; PSC5; ZETA

Reactivity

Human
Predicted: Mouse, Rat

Applications

IF, IHC, IHC, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Western blot analysis of THP1 cell and mouse spleen cell lysate.)

NCF2/NOXA2/p67phox, Polyclonal Antibody (Cat# AAA21281)

Full Name

Anti-NCF2/NOXA2/p67phox Antibody

Gene Names

NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX

Reactivity

Human
Predicted: Mouse, Rat

Applications

ICC, IF, IHC, IHC, IP, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of ASAH1 using anti-ASAH1 antibody.)

ASAH1, Polyclonal Antibody (Cat# AAA19197)

Full Name

Anti-ASAH1 Antibody

Gene Names

ASAH1; AC; PHP; ASAH; PHP32; ACDase; SMAPME

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, EIA

Purity

Immunogen affinity purified.

Pricing

mOrange, Polyclonal Antibody (Cat# AAA27907)

Full Name

mOrange Rabbit Polyclonal Antibody

Applications

WB, IHC, IF, IP

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Pax-5, Polyclonal Antibody (Cat# AAA29216)

Full Name

Pax-5 Polyclonal Antibody

Gene Names

PAX5; BSAP

Reactivity

Human, Mouse

Applications

WB, IHC-P

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

CREB-1, Polyclonal Antibody (Cat# AAA29002)

Full Name

CREB-1 Polyclonal Antibody

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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Application Data (C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)

FGFR2, Polyclonal Antibody (Cat# AAA26853)

Full Name

FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (Biotin)

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, Monkey, Mouse, Rat

Applications

FC/FACS, EIA, IF, IHC, WB

Purity

Purified by Protein G Affinity Chromatography.

Pricing

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Akt, Polyclonal Antibody (Cat# AAA30742)

Full Name

Akt (Phospho-Thr308) Rabbit Polyclonal Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

IF, ICC, WB, IHC-P, EIA

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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Histone Deacetylase 2, Polyclonal Antibody (Cat# AAA29881)

Full Name

Histone Deacetylase 2 Antibody

Gene Names

HDAC2; HD2; RPD3; YAF1

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC, FC/FACS

Purity

Protein affinity purified

Pricing

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.

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Filters

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Reactivity

Gene Name

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Host

Application

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Polyclonal Antibodies

Viewing 100-150 of 3176 product results

HDAC5, Polyclonal Antibody (Cat# AAA19236)

NCF4, Polyclonal Antibody (Cat# AAA10738)

Cyclin A, Polyclonal Antibody (Cat# AAA29005)

PDK4, Polyclonal Antibody (Cat# AAA30723)

RANBP2, Polyclonal Antibody (Cat# AAA19742)

CALR, Polyclonal Antibody (Cat# AAA31108)

Ubiquitin C-terminal Hydrolase 1 (UCHL1) ck, Polyclonal Antibody (Cat# AAA14227)

MIP-3alpha, Polyclonal Antibody (Cat# AAA29367)

TDP-43/TARDBP, Polyclonal Antibody (Cat# AAA19743)

LRRN4, Polyclonal Antibody (Cat# AAA20002)

S-100 alpha, Polyclonal Antibody (Cat# AAA29323)

Acetyl-Histone H4-K12, Polyclonal Antibody (Cat# AAA28313)

KAP-1, Polyclonal Antibody (Cat# AAA23773)

HDAC5, Polyclonal Antibody (Cat# AAA31097)

H7 (H7N9)(A/Shanghai/2/2013), Polyclonal Antibody (Cat# AAA13735)

AXIN2, Polyclonal Antibody (Cat# AAA10910)

Latent Transforming Growth Factor Beta Binding Protein 1, Polyclonal Antibody (Cat# AAA20909)

ENTPD1/CD39, Polyclonal Antibody (Cat# AAA30668)

Chemokine C-X3-C-Motif Ligand 1 (CX3CL1), Polyclonal Antibody (Cat# AAA20087)

Actinin-alpha1/2/3/4, Polyclonal Antibody (Cat# AAA28961)

GSK3 beta, Polyclonal Antibody (Cat# AAA31297)

Bub1, Polyclonal Antibody (Cat# AAA31312)

PAP/ACP3, Polyclonal Antibody (Cat# AAA19446)

Cleaved-Caspase-9, Polyclonal Antibody (Cat# AAA28834)

HA (A/California/06/09)(H1N1), Polyclonal Antibody (Cat# AAA13733)

MGP/Matrix Gla-Protein, Polyclonal Antibody (Cat# AAA21296)

ERK 1/2, Polyclonal Antibody (Cat# AAA28958)

Smad1, Polyclonal Antibody (Cat# AAA22285)

Acetyl-Histone H4-K5/K8/K12/K16, Polyclonal Antibody (Cat# AAA28423)

Acid phosphatase/ACP5, Polyclonal Antibody (Cat# AAA19481)

Annexin I, Polyclonal Antibody (Cat# AAA30753)

MAPK3, Polyclonal Antibody (Cat# AAA10646)

Akt, Polyclonal Antibody (Cat# AAA28965)

Von Willebrand Factor (vWF), Polyclonal Antibody (Cat# AAA20193)

RNASEK, Polyclonal Antibody (Cat# AAA28700)

MX1/MX, Polyclonal Antibody (Cat# AAA21290)

Interferon Alpha, Polyclonal Antibody (Cat# AAA21018)

CCDC59, Polyclonal Antibody (Cat# AAA29808)

Histone H4, Polyclonal Antibody (Cat# AAA29039)

MAP2K5, Polyclonal Antibody (Cat# AAA28148)

RSRC1, Polyclonal Antibody (Cat# AAA10780)

PSMA5, Polyclonal Antibody (Cat# AAA21278)

NCF2/NOXA2/p67phox, Polyclonal Antibody (Cat# AAA21281)

ASAH1, Polyclonal Antibody (Cat# AAA19197)

mOrange, Polyclonal Antibody (Cat# AAA27907)

Pax-5, Polyclonal Antibody (Cat# AAA29216)

CREB-1, Polyclonal Antibody (Cat# AAA29002)

FGFR2, Polyclonal Antibody (Cat# AAA26853)

Akt, Polyclonal Antibody (Cat# AAA30742)

Histone Deacetylase 2, Polyclonal Antibody (Cat# AAA29881)

What are Polyclonal Antibodies?

Key Uses of Polyclonal Antibodies

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

2. Rigorous Quality Control

3. Wide Assortment of Antibodies

4. Highly Purified

FAQ

1. How are polyclonal antibodies produced?

2. How do polyclonal antibodies differ from monoclonal antibodies?

3. How should I store polyclonal antibodies?

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