Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Vas Deferens Tissue; Primary Ab: 10ug/ml Rabbit Anti-Mouse LCN5 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Spleen Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse LCN5 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Ovary Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse LCN5 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot:Sample: Recombinant LCN5, Mouse.)

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Lipocalin 5, Polyclonal Antibody (Cat# AAA20900)

Full Name

Polyclonal Antibody to Lipocalin 5 (LCN5)

Gene Names

Lcn5; Erabp; MEP10; E-RABP

Reactivity

Mus musculus (Mouse)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$240/0.1 mL $290/0.2 mL $490/0.5 mL $585/1 mL $1,580/5 mL $3,070/2x5 mL $4,480/3x5 mL $5,810/4x5 mL $7,100/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Lung cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human MASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Colorectal cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human MASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples: Human Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human MASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human MASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Bile duct cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human MASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples:Human Kidney Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Cholangiocarcinoma Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue.)

WB (Western Blot) (Western Blot; Samples:Lane1: Hela cell lysate;Lane2: LO2 cell lysate;Primary Ab: 0.2ug/ml Rabbit Anti-Human MASP1 AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot: Sample: Recombinant MASP1, Human.)

WB (Western Blot) (Western Blot: Lane1: Human Hela Cells; Lane2: Mouse Liver Tissue.)

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Mannose Associated Serine Protease 1, Polyclonal Antibody (Cat# AAA20912)

Full Name

Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1)

Gene Names

MASP1; 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1

Reactivity

Homo sapiens (Human)

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$220/0.1 mL $265/0.2 mL $415/0.5 mL $505/1 mL $1,340/5 mL $2,600/2x5 mL $3,795/3x5 mL $4,920/4x5 mL $5,995/5x5 mL

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WB (Western Blot) (Western Blot;Sample: Mouse Serum.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Liver Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse C9 AntibodySecond Ab: 2ug/mL HRP Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot:Sample: Recombinant C9, Mouse.)

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Complement Component 9, Polyclonal Antibody (Cat# AAA20913)

Full Name

Polyclonal Antibody to Complement Component 9 (C9)

Reactivity

Mus musculus (Mouse)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$220/0.1 mL $275/0.2 mL $430/0.5 mL $515/1 mL $1,375/5 mL $2,670/2x5 mL $3,895/3x5 mL $5,050/4x5 mL $6,165/5x5 mL

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IF (Immunofluorescence) (Immunofluorescence of TET3 in human brain tissue with TET3 antibody at 20 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of TET3 in human brain tissue with TET3 antibody at 2.5 μg/ml.)

WB (Western Blot) (Western blot analysis of TET3 in SK-N-SH cell lysate with TET3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

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TET3, Polyclonal Antibody (Cat# AAA10939)

Full Name

TET3 Antibody

Gene Names

TET3; hCG_40738

Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB).

Purity

TET3 Antibody is affinity chromatography purified via peptide column.

Pricing

$530/0.1 mg $1,020/2x0.1 mg $1,485/3x0.1 mg $1,925/4x0.1 mg $2,195/5x0.1 mg

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: HumanSkin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human IFI16 Antibody;Second Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Skin Cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Prostate Gland Cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Lung Cancer Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Human Breast Cancer Tissue)

WB (Western Blot) (Western Blot:Sample: Recombinant IFI16, Human.)

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Interferon Gamma Inducible Protein 16, Polyclonal Antibody (Cat# AAA20929)

Full Name

Polyclonal Antibody to Interferon Gamma Inducible Protein 16 (IFI16)

Gene Names

IFI16; PYHIN2; IFNGIP1

Reactivity

Homo sapiens (Human)

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immmunoprecipitation (IP).

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$220/0.1 mL $265/0.2 mL $415/0.5 mL $505/1 mL $1,340/5 mL $2,600/2x5 mL $3,795/3x5 mL $4,920/4x5 mL $5,995/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on fromalin fixed paraffin-embedded Liver tissue)

WB (Western Blot) (Western Blot:Sample: Recombinant FCN2, Mouse.)

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Ficolin 2, Polyclonal Antibody (Cat# AAA20931)

Full Name

Polyclonal Antibody to Ficolin 2 (FCN2)

Gene Names

Fcnb; Fcn2

Reactivity

Mus musculus (Mouse)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$220/0.1 mL $275/0.2 mL $430/0.5 mL $515/1 mL $1,375/5 mL $2,670/2x5 mL $3,895/3x5 mL $5,050/4x5 mL $6,165/5x5 mL

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WB (Western Blot) (Western Blot;Sample: Mouse Cerebrum lysate;Primary Ab: 1ug/ml Rabbit Anti-Human NEFL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot;Sample: Rat Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human NEFL AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot;Sample: Recombinant NEFL, Human.)

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Neurofilament, Light Polypeptide (NEFL), Polyclonal Antibody (Cat# AAA20168)

Full Name

Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL)

Gene Names

NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110

Reactivity

Homo sapiens (Human)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$225/0.1 mL $275/0.2 mL $435/0.5 mL $525/1 mL $1,410/5 mL $2,740/2x5 mL $3,995/3x5 mL $5,180/4x5 mL $6,300/5x5 mL

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IF (Immunofluorescence) (AF488 staining on IF;Sample: NIH/3T3 cellPrimary Ab: 20ug/ml Rabbit Anti-Mouse Kim1 AntibodySecond Ab: 2ug/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot;Sample:Lane 1: 293T cell lysate;Lane 2: A431 cell lysate;Lane 3: A549 cell lysate;Lane 4: Hela cell lysate;Lane 5: MCF7 cell lysate Primary Ab: 0.3ug/ml Rabbit Anti-Mouse Kim1 AntibodySecond Ab: 0.2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

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Kidney Injury Molecule 1 (Kim1), Polyclonal Antibody (Cat# AAA20176)

Full Name

Polyclonal Antibody to Kidney Injury Molecule 1 (Kim1)

Gene Names

Havcr1; Tim1; KIM-1; TIM-1; Timd1; AI503787

Reactivity

Mus musculus (Mouse)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$215/0.1 mL $265/0.2 mL $410/0.5 mL $495/1 mL $1,315/5 mL $2,550/2x5 mL $3,720/3x5 mL $4,825/4x5 mL $5,855/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples:Human Stomach Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Thyroid Tissue)

IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples: Human Kidney Tissue)

IHC (Immunohistochemistry) (HE staining on IHC-P; Samples: Human Liver Tissue)

IHC (Immunohistochemistry) (HE staining on IHC-P; Samples: Human Kidney Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Liver Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant ANXA4, Human.)

WB (Western Blot) (Western Blot: Lane1: Rat Kidney Tissue; Lane2: Human Liver Tissue; Lane3: Rat Prostate Gland Tissue.)

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Annexin A4 (ANXA4), Polyclonal Antibody (Cat# AAA20178)

Full Name

Polyclonal Antibody to Annexin A4 (ANXA4)

Gene Names

ANXA4; ANX4; P32.5; PIG28; PP4-X; ZAP36; PAP-II; HEL-S-274

Reactivity

Human

Applications

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Purity

Affinity Chromatography

Pricing

$225/0.1 mL $275/0.2 mL $435/0.5 mL $525/1 mL $1,410/5 mL $2,740/2x5 mL $3,995/3x5 mL $5,180/4x5 mL $6,300/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)

IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue)

IHC (Immunohistochemistry) (FITC staining on IHC-P; Samples: Human Hela Cells)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant PDHX, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Heart Tissue.)

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Pyruvate Dehydrogenase Complex Component X, Polyclonal Antibody (Cat# AAA20946)

Full Name

Polyclonal Antibody to Pyruvate Dehydrogenase Complex Component X (PDHX)

Gene Names

PDHX; E3BP; OPDX; PDX1; proX; DLDBP; PDHXD

Reactivity

Human

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Purity

Affinity Chromatography

Pricing

$225/0.1 mL $275/0.2 mL $435/0.5 mL $525/1 mL $1,410/5 mL $2,740/2x5 mL $3,995/3x5 mL $5,180/4x5 mL $6,300/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Skin Cancer Tissue)

IHC (Immunohistchemistry) (FITC staining on IHC-P Simple: Hela cells))

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Brain Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Cholangiocarcinoma Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Prostate Gland Cancer Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant NUP50, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human Hela Cells; Lane3: Human HepG2 Cells.)

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Nucleoporin, Polyclonal Antibody (Cat# AAA20959)

Full Name

Polyclonal Antibody to Nucleoporin 50kDa (NUP50)

Gene Names

NUP50; NPAP60; NPAP60L

Reactivity

Human

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $265/0.2 mL $415/0.5 mL $505/1 mL $1,340/5 mL $2,600/2x5 mL $3,795/3x5 mL $4,920/4x5 mL $5,995/5x5 mL

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WB (Western Blot) (25 ug of the indicated Human whole cell extracts was loaded onto a 10-20% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 15 kDa isoform is identified, and a second isoform of 18 kDa is also present in some samples.)

WB (Western Blot) (WB Suggested Anti-PFN1 Antibody Titration: 0.2-1 ug/mlPositive Control: Human Liver)

WB (Western Blot) (Lanes:1. 20 ug murine non-pregnant uteria tissue 2. 20 ug murine non-laboring uterine tissue 3. 20 ug murine laboring uterine tissue 4. 20 ug murine preterm laboring uterine tissuePrimary Antibody Dilution:1:2000Secondary Antibody:Goat anti-rabbit-Alexa Fluor 680Secondary Antibody Dilution:1:25,000Gene Name:PFN1Submitted by:Anonymous)

WB (Western Blot) (Lanes:1. 20 ug human pregnant uterine muscle cells + hormone 2. 20 ug human pregnant uterine muscle cells - hormonePrimary Antibody Dilution:1:2000Secondary Antibody:Goat anti-rabbit-Alexa Fluor 680Secondary Antibody Dilution:1:25,000Gene Name:PFN1Submitted by:Anonymous)

IHC (Immunohistochemistry) (Rabbit Anti-PFN1 antibodyFormalin Fixed Paraffin Embedded Tissue: Human TonsilPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

IHC (Immunohistochemistry) (Rabbit Anti-PFN1 antibodyFormalin Fixed Paraffin Embedded Tissue: Human SpleenPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

IHC (Immunohistochemistry) (Rabbit Anti-PFN1 antibodyFormalin Fixed Paraffin Embedded Tissue: Human LiverPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

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PFN1, Polyclonal Antibody (Cat# AAA23532)

Full Name

PFN1 antibody - N-terminal region

Gene Names

PFN1; ALS18

Reactivity

Tested Species Reactivity: Human, Mouse
Predicted Species Reactivity: Cow, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

WB (Western Blot) (WB Suggested Anti-MOSC1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta)

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MARC1, Polyclonal Antibody (Cat# AAA23536)

Full Name

MARC1 Antibody - C-terminal region

Gene Names

MARC1; MOSC1

Reactivity

Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of formalin-fixed, paraffin-embedded human skin tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Immunohistochemistry of formalin-fixed, paraffin-embedded human spleen tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

WB (Western Blot) (WB Suggested Anti-DBT Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HT1080 cell lysateThere is BioGPS gene expression data showing that DBT is expressed in HT1080)

WB (Western Blot) (Host: RabbitTarget: DBTPositive control (+): Human Liver (LI)Negative control (-): HeLa Cell Lysate (HL)Antibody concentration: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: DBTSample Tissue: Human THP-1 Whole CellAntibody Dilution: 0.5ug/ml)

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DBT, Polyclonal Antibody (Cat# AAA23544)

Full Name

DBT antibody - N-terminal region

Gene Names

DBT; E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCOADC-E2

Reactivity

Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Hela cells using anti-Aquaporin 3 antibody (AAA11516).Overlay histogram showing Hela cells stained with AAA11516 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 3 Antibody (AAA11516,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (AAA11516). Aquaporin 3 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (AAA11516) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (AAA11516). Aquaporin 3 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (AAA11516) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (AAA11516). Aquaporin 3 was detected in frozen section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (AAA11516) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (AAA11516). Aquaporin 3 was detected in immunocytochemical section of HELA cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (AAA11516) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (AAA11516). Aquaporin 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (AAA11516) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (AAA11516). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Kidney Tissue Lysate Lane 2: Rat Lung Tissue Lysate Lane 3: Mouse Kidney Tissue Lysate Lane 4: MM453 Whole Cell Lysate Lane 5: SMMC-7721 Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Aquaporin 3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Aquaporin 3 at approximately 32KD. The expected band size for Aquaporin 3 is at 32KD.)

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Aquaporin 3, Polyclonal Antibody (Cat# AAA11516)

Full Name

Anti-Aquaporin 3 antibody

Gene Names

AQP3; GIL; AQP-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse cerebellum was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using 1:100 dilution. Immuno-reactivity is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse olfactory lobe was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using a 1:100 dilution. Reactivity is mostly nuclear.)

IS (Immunostaining) (Paraffin-embedded human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

IS (Immunostaining) (Paraffin-embedded mouse brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

WB (Western Blot) (Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with (1:10,000) dilution. Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi-luminescence detection system. Arrow indicates Tbr2 (~73 kD).)

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EOMES, Polyclonal Antibody (Cat# AAA26883)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by affinity chromatography.

Pricing

$1,265/0.1 mL $2,455/2x0.1 mL $3,580/3x0.1 mL $4,640/4x0.1 mL $5,465/5x0.1 mL

WB (Western Blot) (Western blot analysis of mOrange recombinant protein with E12-176 diluted at 1:5, 000.)

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mOrange, Polyclonal Antibody (Cat# AAA27907)

Full Name

mOrange Rabbit Polyclonal Antibody

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Pricing

$385/0.1 mg $565/0.1 mL (AF350) $565/0.1 mL (AF405L) $565/0.1 mL (AF405M) $565/0.1 mL (AF405S) $565/0.1 mL (AF488) $565/0.1 mL (AF546) $565/0.1 mL (AF555) $565/0.1 mL (AF568) $565/0.1 mL (AF594) $565/0.1 mL (AF610) $565/0.1 mL (AF635) $565/0.1 mL (AF647) $565/0.1 mL (AF680) $565/0.1 mL (AF750) $565/0.1 mL (Biotin) $565/0.1 mL (FITC) $565/0.1 mL (HRP) $695/0.1 mL (AP) $695/0.1 mL (APC) $695/0.1 mL (Cy3) $695/0.1 mL (Cy5) $695/0.1 mL (Cy5.5) $695/0.1 mL (Cy7) $695/0.1 mL (PE) $1,345/2x0.1 mL (AP) $1,955/3x0.1 mL (AP) $2,535/4x0.1 mL (AP)

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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SNAP 23, Polyclonal Antibody (Cat# AAA29187)

Full Name

SNAP 23 Polyclonal Antibody

Gene Names

SNAP23; SNAP-23; SNAP23A; SNAP23B; HsT17016

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with PUMA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining PUMA in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PUMA in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PUMA in SKOV-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PUMA on different cell lysates using anti- PUMA antibody at 1/1000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse kidney)

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PUMA, Polyclonal Antibody (Cat# AAA29955)

Full Name

PUMA Antibody

Gene Names

BBC3; JFY1; PUMA; JFY-1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human rectum using PA2G4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using PA2G4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using PA2G4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using PA2G4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using PA2G4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using PA2G4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using PA2G4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using PA2G4 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PA2G4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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PA2G4, Polyclonal Antibody (Cat# AAA28168)

Full Name

PA2G4 Polyclonal Antibody

Gene Names

PA2G4; EBP1; HG4-1; p38-2G4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), RIP

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using TRAP1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using TRAP1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using TRAP1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using TRAP1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat skeletal muscle using TRAP1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using TRAP1 Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TRAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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TRAP1, Polyclonal Antibody (Cat# AAA10761)

Full Name

TRAP1 Polyclonal Antibody

Gene Names

TRAP1; HSP75; HSP 75; HSP90L; TRAP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HepG2 cells using anti-IFT52 antibody (AAA19977).Overlay histogram showing HepG2 cells stained with AAA19977 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IFT52 Antibody (AAA19977, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of IFT52 using anti-IFT52 antibody (AAA19977).IFT52 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-IFT52 Antibody (AAA19977) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of IFT52 using anti-IFT52 antibody (AAA19977).IFT52 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT52 Antibody (AAA19977) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT52 Antibody (AAA19977) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT52 Antibody (AAA19977) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT52 Antibody (AAA19977) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat testis tissue lysates,Lane 3: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT52 antigen affinity purified polyclonal antibody (#AAA19977) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFT52 at approximately 45 kDa. The expected band size for IFT52 is at 50 kDa.)

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IFT52, Polyclonal Antibody (Cat# AAA19977)

Full Name

Anti-IFT52 Antibody Picoband

Gene Names

IFT52; NGD2; NGD5; CGI-53; C20orf9; dJ1028D15.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-IDH1 Antibody Titration: 1 ug/mlPositive Control: Fetal liver cell lysate)

WB (Western Blot) (Host: RatTarget Name: IDH1Sample Tissue: Rat LiverAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: IDH1Sample Type: Human MCF7Antibody Dilution: 1.0ug/mlIDH1 is supported by BioGPS gene expression data to be expressed in MCF7)

WB (Western Blot) (Host: RabbitTarget Name: IDH1Sample Type: Human HepG2Antibody Dilution: 1.0ug/mlIDH1 is supported by BioGPS gene expression data to be expressed in HepG2)

IHC (Immunohistochemistry) (Human Testis)

IHC (Immunohistochemistry) (Human Testis)

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IDH1, Polyclonal Antibody (Cat# AAA23561)

Full Name

IDH1 antibody - C-terminal region

Gene Names

IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse cerebellum was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using 1:100 dilution. Immuno-reactivity is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse olfactory lobe was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using a 1:100 dilution. Reactivity is mostly nuclear.)

IS (Immunostaining) (Paraffin-embedded human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

IS (Immunostaining) (Paraffin-embedded mouse brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

WB (Western Blot) (Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with (1:10,000) dilution. Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi-luminescence detection system. Arrow indicates Tbr2 (~73 kD).)

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EOMES, Polyclonal Antibody (Cat# AAA26889)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by affinity chromatography.

Pricing

$1,285/0.1 mL $2,495/2x0.1 mL $3,635/3x0.1 mL $4,715/4x0.1 mL $5,565/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

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IRS-1, Polyclonal Antibody (Cat# AAA28930)

Full Name

IRS-1 (phospho Ser636) Polyclonal Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using HLA-A antibody. Blue: DAPI for nuclear staining)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver using HLA-A antibody at dilution of 1:500 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney using HLA-A antibody at dilution of 1:500 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human neurilemmoma using HLA-A antibody at dilution of 1:500 (200x lens)..)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human endometrial cancer using HLA-A antibody at dilution of 1:500 (200x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HLA-A antibody.)

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HLA-A, Polyclonal Antibody (Cat# AAA29698)

Full Name

HLA-A Antibody

Gene Names

HLA-A; HLAA

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using NF-kB p65 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using NF-kB p65 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using NF-kB p65 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using NF-kB p65 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using NF-kB p65 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using NF-kB p65 Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NF-kB p65 antibody.)

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NF-kB p65, Polyclonal Antibody (Cat# AAA30722)

Full Name

NF-kB p65 Rabbit Polyclonal Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Figure 1. Western blot analysis of NHEJ1 using anti-NHEJ1 antibody.)

IHC (Immunohistchemistry)

IHC (Immunohistochemistry)

IF (Immunofluorescence)

IF (Immunofluorescence)

FCM (Flow Cytometry)

FCM (Flow Cytometry)

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XLF/NHEJ1, Polyclonal Antibody (Cat# AAA19203)

Full Name

Anti-XLF/NHEJ1 Antibody

Gene Names

NHEJ1; XLF

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.