Filters
▼Clonality
▼Type
▼Reactivity
▼Gene Name
▼Isotype
▼Host
▼Application
▼Clone
▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2150-2200 of 10000 product results
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)
Caspase-3, Polyclonal Antibody (Cat# AAA29177)
Full Name
Caspase-3 Polyclonal Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of MELTF using anti-MELTF antibody (AAA19980).MELTF was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat C6 whole cell lysates,Lane 3: mouse skin tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MELTF antigen affinity purified polyclonal antibody (#AAA19980) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human A431 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MELTF antigen affinity purified polyclonal antibody (#AAA19980) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MELTF at approximately 90 kDa. The expected band size for MELTF is at 80 kDa.)
MELTF, Polyclonal Antibody (Cat# AAA19980)
Full Name
Anti-MELTF Antibody Picoband
Gene Names
MFI2; MTF1; CD228; MAP97
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-PRPSAP2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:100Positive Control: HepG2 cell lysate)
PRPSAP2, Polyclonal Antibody (Cat# AAA23567)
Full Name
PRPSAP2 Antibody - N-terminal region
Gene Names
PRPSAP2; PAP41
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of L1CAM using anti-L1CAM antibody (AAA19138).L1CAM was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-L1CAM Antibody (AAA19138) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
L1CAM, Polyclonal Antibody (Cat# AAA19138)
Full Name
Anti-L1CAM antibody
Gene Names
L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of SEC61G using anti-SEC61G antibody (AAA19974).SEC61G was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SEC61G Antibody (AAA19974) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SEC61G Antibody (AAA19974) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SEC61G Antibody (AAA19974) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SEC61G Antibody (AAA19974) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SEC61G Antibody (AAA19974) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U87 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human U251 whole cell lysates,Lane 5: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC61G antigen affinity purified polyclonal antibody (#AAA19974) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SEC61G at approximately 12 kDa. The expected band size for SEC61G is at 8 kDa.)
SEC61G, Polyclonal Antibody (Cat# AAA19974)
Full Name
Anti-SEC61G Antibody Picoband
Gene Names
SEC61G; SSS1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of JK cells using anti-MED4 antibody (AAA19872).Overlay histogram showing JK cells stained with AAA19872 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MED4 Antibody (AAA19872, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MED4, Polyclonal Antibody (Cat# AAA19872)
Full Name
Anti-MED4 Antibody Picoband
Gene Names
MED4; ARC36; VDRIP; DRIP36; TRAP36; HSPC126
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-PLXDC2 antibody (AAA19912).Overlay histogram showing MCF-7 cells stained with AAA19912 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLXDC2 Antibody (AAA19912, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PLXDC2, Polyclonal Antibody (Cat# AAA19912)
Full Name
Anti-PLXDC2 Antibody Picoband
Gene Names
PLXDC2; TEM7R
Reactivity
Human
Applications
EIA, FC/FACS, IHC, WB
Purity
Immunogen affinity purified.
Pricing
Application Data
(Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300. Not yet tested in other applications.)
Nrf2, Polyclonal Antibody (Cat# AAA28894)
Full Name
Nrf2 Polyclonal Antibody
Gene Names
NFE2L2; NRF2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
GAPDH, Polyclonal Antibody (Cat# AAA28397)
Full Name
GAPDH Rabbit pAb
Gene Names
GAPDH; G3PD; GAPD
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of RAW264. 7 cells using anti-TRIM25/EFP antibody (AAA19272).Overlay histogram showing RAW264. 7 cells stained with AAA19272 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM25/EFP Antibody (AAA19272, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TRIM25/EFP, Polyclonal Antibody (Cat# AAA19272)
Full Name
Anti-TRIM25/EFP Antibody
Gene Names
Trim25; EFP; Zfp147; AA960166; AL022677
Reactivity
Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U2OS cells using anti-SAP18 antibody (AAA19568).Overlay histogram showing U2OS cells stained with AAA19568 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAP18 Antibody (AAA19568, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SAP18, Polyclonal Antibody (Cat# AAA19568)
Full Name
Anti-SAP18 Antibody Picoband
Gene Names
SAP18; SAP18P; 2HOR0202
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HEL cells using anti-NDUFB8 antibody (AAA19591).Overlay histogram showing HEL cells stained with AAA19591 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB8 Antibody (AAA19591, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFB8, Polyclonal Antibody (Cat# AAA19591)
Full Name
Anti-NDUFB8 Antibody Picoband
Gene Names
NDUFB8; ASHI; CI-ASHI
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
CTPS, Polyclonal Antibody (Cat# AAA29004)
Full Name
CTPS Polyclonal Antibody
Gene Names
CTPS1; CTPS; IMD24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using DNAJB1 at dilution of 1:100. Blue: DAPI for nuclear staining.)
DNAJB1, Polyclonal Antibody (Cat# AAA29711)
Full Name
DNAJB1 Antibody
Gene Names
DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using ECH1 antibody.)
ECH1, Polyclonal Antibody (Cat# AAA29803)
Full Name
ECH1 Polyclonal Antibody
Gene Names
ECH1; HPXEL
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot of rat brain lysate showing specific immunolabeling of SHANK1 at 1:500 dilution.)
SHANK1a, Polyclonal Antibody (Cat# AAA14230)
Full Name
Anti-SHANK1a (C-terminus) Antibody
Gene Names
SHANK1; SSTRIP; SPANK-1; synamon
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Raji cells using anti-CD38 antibody (AAA19217).Overlay histogram showing Raji cells stained with AAA19217 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD38 Antibody (AAA19217, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD38, Polyclonal Antibody (Cat# AAA19217)
Full Name
Anti-CD38 Antibody
Gene Names
CD38; T10; ADPRC 1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
CD236, Polyclonal Antibody (Cat# AAA29364)
Full Name
CD236 Polyclonal Antibody
Gene Names
GYPC; GE; GPC; GPD; GYPD; CD236; PAS-2; CD236R; PAS-2'
Reactivity
Human
Applications
Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using ZNF581 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
ZNF581, Polyclonal Antibody (Cat# AAA22179)
Full Name
ZNF581 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-PFKP antibody (AAA19883).Overlay histogram showing MCF-7 cells stained with AAA19883 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFKP Antibody (AAA19883, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PFKP, Polyclonal Antibody (Cat# AAA19883)
Full Name
Anti-PFKP Antibody Picoband
Gene Names
PFKP; PFKF; PFK-C
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
AP-1, Polyclonal Antibody (Cat# AAA28921)
Full Name
AP-1 (phospho Ser63) Polyclonal Antibody
Gene Names
JUN; AP1; AP-1; c-Jun
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of HepG2 cells using anti-SGK493/PKDCC antibody (AAA19960).Overlay histogram showing HepG2 cells stained with AAA19960 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGK493/PKDCC Antibody (AAA19960, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SGK493/PKDCC, Polyclonal Antibody (Cat# AAA19960)
Full Name
Anti-SGK493/PKDCC Antibody Picoband
Gene Names
PKDCC; Vlk; SGK493
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using ID3 antibody.)
ID3, Polyclonal Antibody (Cat# AAA28175)
Full Name
ID3 Polyclonal Antibody
Gene Names
ID3; HEIR-1; bHLHb25
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cells using SOX5 antibody.)
SOX5, Polyclonal Antibody (Cat# AAA29833)
Full Name
SOX5 Polyclonal Antibody
Gene Names
SOX5; L-SOX5; LAMSHF; L-SOX5B; L-SOX5F
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
HDC, Polyclonal Antibody (Cat# AAA29186)
Full Name
HDC Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
DB (Dot Blot)
(Dot blot analysis using Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507) at 1:500, 1:5000. Beta Amyloid HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.)
Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17802)
Full Name
Amyloid Fibrils (OC) Antibody: RPE
Reactivity
Human. Potentially mouse and rat based on species homology.
Applications
IP, ICC, IHC, EIA, WB, DB
Pricing
FCM (Flow Cytometry)
(Figure 12. Flow Cytometry analysis of U20S cells using anti-HAGH antibody (AAA19915).Overlay histogram showing U20S cells stained with AAA19915 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HAGH Antibody (AAA19915, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HAGH, Polyclonal Antibody (Cat# AAA19915)
Full Name
Anti-HAGH Antibody Picoband
Gene Names
HAGH; GLO2; GLX2; GLXII; HAGH1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using Raptor Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
Raptor, Polyclonal Antibody (Cat# AAA22260)
Full Name
Raptor Polyclonal Antibody
Gene Names
RPTOR; KOG1; Mip1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using UHRF1 antibody. Blue: DAPI for nuclear staining.)
UHRF1, Polyclonal Antibody (Cat# AAA10713)
Full Name
UHRF1 Polyclonal Antibody
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; hUHRF1; huNp95
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of K562 cells using anti-IRF3 antibody (AAA19216).Overlay histogram showing K562 cells stained with AAA19216 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRF3 Antibody (AAA19216, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IRF3, Polyclonal Antibody (Cat# AAA19216)
Full Name
Anti-IRF3 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
MRE11, Polyclonal Antibody (Cat# AAA29072)
Full Name
MRE11 Polyclonal Antibody
Gene Names
MRE11A; ATLD; HNGS1; MRE11; MRE11B
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
IL-12A p35, Polyclonal Antibody (Cat# AAA29257)
Full Name
IL-12A p35 Polyclonal Antibody
Gene Names
IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunohistochemistry of paraffin-embedded human pancreas using MLH1 Mouse mAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
MLH1, Polyclonal Antibody (Cat# AAA28421)
Full Name
MLH1 Mouse mAb
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
NTE, Polyclonal Antibody (Cat# AAA29234)
Full Name
NTE Polyclonal Antibody
Gene Names
PNPLA6; NTE; sws; SPG39; NTEMND; iPLA2delta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat lung using SUZ12 antibody.)
SUZ12, Polyclonal Antibody (Cat# AAA29851)
Full Name
SUZ12 Polyclonal Antibody
Gene Names
SUZ12; CHET9; JJAZ1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using VDAC2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
VDAC2, Polyclonal Antibody (Cat# AAA22267)
Full Name
VDAC2 Polyclonal Antibody
Gene Names
VDAC2; POR
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-Paxillin/PXN antibody (AAA19417).Overlay histogram showing MCF-7 cells stained with AAA19417 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Paxillin/PXN Antibody (AAA19417, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Paxillin/PXN, Polyclonal Antibody (Cat# AAA19417)
Full Name
Anti-Paxillin/PXN Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-PGC antibody (AAA19725).Overlay histogram showing HepG2 cells stained with AAA19725 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PGC Antibody (AAA19725, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PGC, Polyclonal Antibody (Cat# AAA19725)
Full Name
Anti-PGC Antibody Picoband
Gene Names
PGC; PEPC; PGII
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blotAll lanes: Beta-defensin 14 antibody at 12ug/ml+ Rat liver tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 8 kDaObserved band size: 8 kDa)
Beta-defensin 14, Polyclonal Antibody (Cat# AAA18814)
Full Name
Rabbit anti-mouse Beta-defensin 14 polyclonal Antibody
Gene Names
Defb14; BD-14
Reactivity
Mouse, Rat
Applications
EIA, WB
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of SiHa cells using anti-Aconitase 1/ACO1 antibody (AAA19267).Overlay histogram showing SiHa cells stained with AAA19267 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aconitase 1/ACO1 Antibody (AAA19267,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Aconitase 1/ACO1, Polyclonal Antibody (Cat# AAA19267)
Full Name
Anti-Aconitase 1/ACO1 Antibody
Gene Names
ACO1; IRP1; ACONS; IREB1; IREBP; IREBP1
Reactivity
Human, Mouse, Monkey, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of HeLa cells using anti-ACADM/MCAD antibody (AAA19455).Overlay histogram showing HeLa cells stained with AAA19455 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACADM/MCAD Antibody (AAA19455, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ACADM/MCAD, Polyclonal Antibody (Cat# AAA19455)
Full Name
Anti-ACADM/MCAD Antibody Picoband
Gene Names
ACADM; MCAD; ACAD1; MCADH
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of A431 cells using anti-LSM8 antibody (AAA19337).Overlay histogram showing A431 cells stained with AAA19337 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LSM8 Antibody (AAA19337, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
LSM8, Polyclonal Antibody (Cat# AAA19337)
Full Name
Anti-LSM8 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of C6 cells using anti-VCP antibody (AAA19400).Overlay histogram showing C6 cells stained with AAA19400 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCP Antibody (AAA19400, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
VCP, Polyclonal Antibody (Cat# AAA19400)
Full Name
Anti-VCP Antibody Picoband
Gene Names
VCP; p97; TERA; ALS14; IBMPFD
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Rat bronchus cells using KRT7 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
KRT7, Polyclonal Antibody (Cat# AAA22219)
Full Name
KRT7 Polyclonal Antibody
Gene Names
KRT7; K7; CK7; SCL; K2C7
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells usingTFEB Rabbit pAb (AAA28118) at dilution of 1:50 (40xlens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG(H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
TFEB, Polyclonal Antibody (Cat# AAA28118)
Full Name
TFEB Polyclonal Antibody
Gene Names
TFEB; TCFEB; BHLHE35; ALPHATFEB
Applications
WB, IHC-p, IF, ICC, EIA
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse stomach using TRMT2A antibody at dilution of 1:100 (40x lens).)
TRMT2A, Polyclonal Antibody (Cat# AAA28127)
Full Name
TRMT2A Polyclonal Antibody
Gene Names
TRMT2A; HTF9C
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26857)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (APC)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
WB (Western Blot)
(WB Suggested Anti-HSPA8 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate)
HSPA8, Polyclonal Antibody (Cat# AAA23533)
Full Name
HSPA8 antibody - N-terminal region
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
Reactivity
Cow, Goat, Human, Mouse, Pig, Rat, Sheep, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cell using MAPK1 antibody. Blue: DAPI for nuclear staining.)
MAPK1, Polyclonal Antibody (Cat# AAA29678)
Full Name
MAPK1 Antibody
Gene Names
MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
WB (Western Blot)
(Western Blot;Sample: Recombinant GP1Ba, Mouse.)
Glycoprotein Ib Alpha, Polyclonal Antibody (Cat# AAA20934)
Full Name
Polyclonal Antibody to Glycoprotein Ib Alpha Polypeptide, Platelet (GP1Ba)
Gene Names
Gp1ba; GPIba; GPIbalpha
Reactivity
Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.