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Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.


Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.


Viewing 2350-2400 of 10000 product results


product-image-AAA27896_IF3.png IF (Immunofluorescence) (Immunofluorescent analysis of SLC39A8 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

SLC39A8, Polyclonal Antibody (Cat# AAA27896)

Full Name
Anti-SLC39A8 Antibody
Gene Names
SLC39A8; ZIP8; PP3105; BIGM103; LZT-Hs6
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC
Purity
Purified by Immunogen Affinity Chromatography
Pricing
product-image-AAA19404_FCM7.png FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Jurkat cells using anti-TPR antibody (AAA19404).Overlay histogram showing Jurkat cells stained with AAA19404 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TPR Antibody (AAA19404, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TPR, Polyclonal Antibody (Cat# AAA19404)

Full Name
Anti-TPR Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
product-image-AAA22198_IF8.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

ERK1/ERK2, Polyclonal Antibody (Cat# AAA22198)

Full Name
ERK1/ERK2 Polyclonal Antibody
Gene Names
MAPK1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
product-image-AAA29670_IF7.jpg IF (Immunofluorescence) (Immunofluorescence analysis of A549 cell using BCL2 antibody. Blue: DAPI for nuclear staining.)

BCL2, Polyclonal Antibody (Cat# AAA29670)

Full Name
BCL2 Antibody
Reactivity
Human
Applications
WB, IHC, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
product-image-AAA29705_IHC8.jpg IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human liver fibrosis tissue using PELP1 antibody at dilution of 1:200 (x400 lens))

PELP1, Polyclonal Antibody (Cat# AAA29705)

Full Name
PELP1 antibody
Gene Names
PELP1; MNAR; P160
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
product-image-AAA19301_FCM7.png FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U20S cells using anti-VDAC3 antibody (AAA19301).Overlay histogram showing U20S cells stained with AAA19301 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VDAC3 Antibody (AAA19301, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

VDAC3, Polyclonal Antibody (Cat# AAA19301)

Full Name
Anti-VDAC3 Antibody
Gene Names
VDAC3; VDAC-3; HD-VDAC3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19903_FCM6.png FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HepG2 cells using anti-PFKFB1 antibody (AAA19903).Overlay histogram showing HepG2 cells stained with AAA19903 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFKFB1 Antibody (AAA19903, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PFKFB1, Polyclonal Antibody (Cat# AAA19903)

Full Name
Anti-PFKFB1 Antibody Picoband
Gene Names
PFKFB1; F6PK; HL2K; PFRX
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA28123_IF9.jpg IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using TOMM20 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

TOMM20, Polyclonal Antibody (Cat# AAA28123)

Full Name
TOMM20 Polyclonal Antibody
Gene Names
TOMM20; MAS20; MOM19; TOM20
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity Purification
Pricing
product-image-AAA19453_FCM7.jpg FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HeLa cells using anti-delta 1 Catenin/CAS/CTNND1 antibody (AAA19453).Overlay histogram showing HeLa cells stained with AAA19453 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-delta 1 Catenin/CAS/CTNND1 Antibody (AAA19453, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

delta 1 Catenin/CAS/CTNND1, Polyclonal Antibody (Cat# AAA19453)

Full Name
Anti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband
Gene Names
CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19879_FCM9.jpg FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of THP-1 cells using anti-QARS1 antibody (AAA19879).Overlay histogram showing THP-1 cells stained with AAA19879 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-QARS1 Antibody (AAA19879, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

QARS1, Polyclonal Antibody (Cat# AAA19879)

Full Name
Anti-QARS1 Antibody Picoband
Gene Names
QARS; GLNRS; MSCCA; PRO2195
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA26864_IF6.jpg IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

OPN-a/b, Polyclonal Antibody (Cat# AAA26864)

Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 750)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
product-image-AAA19199_WB7.jpg WB (Western Blot) (Figure 1. Western blot analysis of MRC1 using anti-MRC1 antibody.)

Mannose Receptor/MRC1, Polyclonal Antibody (Cat# AAA19199)

Full Name
Anti-Mannose Receptor/MRC1 Antibody
Gene Names
MRC1; MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA23487_WB7.jpg WB (Western Blot) (WB Suggested Anti-RBM26 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that RBM26 is expressed in HepG2)

RBM26, Polyclonal Antibody (Cat# AAA23487)

Full Name
RBM26 antibody - middle region
Gene Names
RBM26; ARRS2; SE70-2; ZC3H17; PRO1777; C13orf10; PPP1R132
Reactivity
Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
product-image-AAA19909_IF8.jpg IF (Immunofluorescence) (Figure 8. IF analysis of ATP4B using anti-ATP4B antibody (AAA19909).ATP4B was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-ATP4B Antibody (AAA19909) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

ATP4B, Polyclonal Antibody (Cat# AAA19909)

Full Name
Anti-ATP4B Antibody Picoband
Gene Names
ATP4B; ATP6B
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
product-image-AAA28386_IHC6.jpg IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using RAB11A/RAB11B Rabbit pAb at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

RAB11A/RAB11B, Polyclonal Antibody (Cat# AAA28386)

Full Name
RAB11A/RAB11B Rabbit pAb
Gene Names
RAB11A; YL8
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
product-image-AAA19943_FCM9.png FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of 293T cells using anti-IDH3G antibody (AAA19943).Overlay histogram showing 293T cells stained with AAA19943 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDH3G Antibody (AAA19943, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IDH3G, Polyclonal Antibody (Cat# AAA19943)

Full Name
Anti-IDH3G Antibody Picoband
Gene Names
IDH3G; H-IDHG
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19953_FCM9.png FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-MOGAT2 antibody (AAA19953).Overlay histogram showing HepG2 cells stained with AAA19953 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOGAT2 Antibody (AAA19953, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MOGAT2, Polyclonal Antibody (Cat# AAA19953)

Full Name
Anti-MOGAT2 Antibody Picoband
Gene Names
MOGAT2; MGAT2; DGAT2L5
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, ICC, WB, FC/FACS
Purity
Immunogen affinity purified.
Pricing
product-image-AAA28927_IHC7.jpg IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

FoxO3A, Polyclonal Antibody (Cat# AAA28927)

Full Name
FoxO3A (phospho Ser253) Polyclonal Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
product-image-AAA20838_IHC6.jpg IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples: Mouse Ovary Tissue.)

Microsomal Glutathione S Transferase 1, Polyclonal Antibody (Cat# AAA20838)

Full Name
Polyclonal Antibody to Microsomal Glutathione S Transferase 1 (MGST1)
Gene Names
Mgst1; Gst; 1500002K10Rik
Reactivity
Mouse, Rat
Applications
WB, ICC, IHC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Pricing
product-image-AAA19770_FCM9.png FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U87 cells using anti-PKP2 antibody (AAA19770).Overlay histogram showing U87 cells stained with AAA19770 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKP2 Antibody (AAA19770, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PKP2, Polyclonal Antibody (Cat# AAA19770)

Full Name
Anti-PKP2 Antibody Picoband
Gene Names
PKP2; ARVD9
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19987_FCM8.png FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-PRDM15 antibody (AAA19987).Overlay histogram showing A431 cells stained with AAA19987 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM15 Antibody (AAA19987, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PRDM15, Polyclonal Antibody (Cat# AAA19987)

Full Name
Anti-PRDM15 Antibody Picoband
Gene Names
PRDM15; PFM15; ZNF298; C21orf83
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA22176_IF7.jpg IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HSP90AA1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

HSP90AA1, Polyclonal Antibody (Cat# AAA22176)

Full Name
HSP90AA1 Polyclonal Antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
product-image-AAA19160_FCM8.jpg FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U20S cells using anti-Dynamin 1 antibody (AAA19160).Overlay histogram showing U20S cells stained with AAA19160 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1 Antibody (AAA19160,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Dynamin 1, Polyclonal Antibody (Cat# AAA19160)

Full Name
Anti-Dynamin 1 Picoband antibody
Gene Names
DNM1; DNM; EIEE31
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
product-image-AAA29939_FCM8.jpg FCM (Flow Cytometry) (Flow cytometric analysis of MG-63 cells with Kv4.3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody)

KV4.3, Polyclonal Antibody (Cat# AAA29939)

Full Name
KV4.3 Antibody
Gene Names
KCND3; KV4.3; SCA19; SCA22; KCND3L; KCND3S; KSHIVB
Reactivity
Human, Mouse
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
product-image-AAA19967_FCM9.jpg FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U937 cells using anti-Syntaxin 18/STX18 antibody (AAA19967).Overlay histogram showing U937 cells stained with AAA19967 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

Syntaxin 18/STX18, Polyclonal Antibody (Cat# AAA19967)

Full Name
Anti-Syntaxin 18/STX18 Antibody Picoband
Gene Names
STX18; Ufe1
Reactivity
Human
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
product-image-AAA28413_IF6.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Smad1, Polyclonal Antibody (Cat# AAA28413)

Full Name
[KO Validated] Smad1 Rabbit pAb
Reactivity
Human
Applications
WB, IHC, IF, ICC
Purity
Affinity purification
Pricing
product-image-AAA28414_IF7.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 using Heme Oxygenase 1 (HO-1/HMOX1) antibody at dilution of 1 : 50 (40x lens). Blue: DAPI for nuclear staining.)

Heme Oxygenase 1 (HO-1/HMOX1), Polyclonal Antibody (Cat# AAA28414)

Full Name
[KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb
Gene Names
NR1D2; RVR; BD73; EAR-1R
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC
Purity
Affinity purification
Pricing
product-image-AAA20093_IHC5.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Uterine Fibroids Tissue)

Chymase 1, Mast Cell (CMA1), Polyclonal Antibody (Cat# AAA20093)

Full Name
Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)
Gene Names
Cma1; Mcp5; Mcp-5; Mcpt5; MMCP-5
Reactivity
Human, Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
product-image-AAA19606_FCM8.png FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of Caco-2 cells using anti-TOMM70 antibody (AAA19606).Overlay histogram showing Caco-2 cells stained with AAA19606 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM70 Antibody (AAA19606, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TOMM70, Polyclonal Antibody (Cat# AAA19606)

Full Name
Anti-TOMM70 Antibody Picoband
Reactivity
Human
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19333_FCM6.png FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-NDUFB5 antibody (AAA19333).Overlay histogram showing A431 cells stained with AAA19333 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB5 Antibody (AAA19333, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NDUFB5, Polyclonal Antibody (Cat# AAA19333)

Full Name
Anti-NDUFB5 Antibody
Gene Names
NDUFB5; SGDH; CISGDH
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19325_FCM7.png FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Hela cells using anti- ETF/TEAD2 antibody (AAA19325).Overlay histogram showing Hela cells stained withAAA19325 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- ETF/TEAD2 Antibody (AAA19325, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ETF/TEAD2, Polyclonal Antibody (Cat# AAA19325)

Full Name
Anti-ETF/TEAD2 Antibody
Gene Names
TEAD2; ETF; TEF4; TEF-4; TEAD-2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19652_FCM12.jpg FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of 293T cells using anti-ATP5F1B antibody (AAA19652).Overlay histogram showing 293T cells stained with AAA19652 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5F1B Antibody (AAA19652, 1 ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ATP5F1B, Polyclonal Antibody (Cat# AAA19652)

Full Name
Anti-ATP5F1B Antibody Picoband
Gene Names
ATP5F1B; ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA29213_IHC7.jpg IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

CMTM6, Polyclonal Antibody (Cat# AAA29213)

Full Name
CMTM6 Polyclonal Antibody
Gene Names
CMTM6; CKLFSF6; PRO2219
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
product-image-AAA17816_IHC2.jpg IHC (Immunohistochemistry) (Immunohistochemical analysis of Laminin alpha 5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Laminin alpha 5, Polyclonal Antibody (Cat# AAA17816)

Full Name
Anti-Laminin alpha 5 Antibody
Reactivity
Human, Mouse
Applications
WB, IHC, IP
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
product-image-AAA18870_ChIP6.jpg ChIP (Chromatin Immunoprecipitation) (Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-MCM3 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the IRF-1 promoter.)

DNA replication licensing factor MCM3, Polyclonal Antibody (Cat# AAA18870)

Full Name
Rabbit anti-human DNA replication licensing factor MCM3 polyclonal Antibody(MCM3)
Gene Names
MCM3; HCC5; P1.h; RLFB; P1-MCM3
Reactivity
Human
Applications
EIA, WB, IHC, ChIP
Purity
Antigen Affinity Purified
Pricing
product-image-AAA26871_WB7.jpg WB (Western Blot) (Western Blot analysis of FKBP4 expression in transfected 293T cell line by FKBP4 polyclonal antibody. Lane 1: FKBP4 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)

FKBP4, Polyclonal Antibody (Cat# AAA26871)

Full Name
FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) (PE)
Gene Names
FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
Reactivity
Human, Mouse
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
product-image-AAA19323_FCM11.png FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of THP-1 cells using anti-CDC123 antibody (AAA19323).Overlay histogram showing THP-1 cells stained with AAA19323 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC123 Antibody (AAA19323, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CDC123, Polyclonal Antibody (Cat# AAA19323)

Full Name
Anti-CDC123 Antibody
Gene Names
CDC123; D123; C10orf7
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19549_IHC8.jpg IHC (Immunohistochemistry) (Figure 8. IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).COX IV/COX4I1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COX IV/COX4I1 Antibody (AAA19549) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

COX IV/COX4I1, Polyclonal Antibody (Cat# AAA19549)

Full Name
Anti-COX IV/COX4I1 Antibody Picoband
Gene Names
COX4I1; COX4; COXIV; COX4-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19147_IHC7.jpg IHC (Immunohistochemistry) (Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (AAA19147).VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 3 Antibody (AAA19147) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

VEGF Receptor 3, Polyclonal Antibody (Cat# AAA19147)

Full Name
Anti-VEGF Receptor 3 Picoband antibody
Gene Names
FLT4; PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB, FC
Purity
Immunoggen affinity purified
Pricing
product-image-AAA22250_IF7.jpg IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using AIF Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

AIF, Polyclonal Antibody (Cat# AAA22250)

Full Name
AIF Polyclonal Antibody
Gene Names
AIFM1; AIF; CMTX4; COWCK; PDCD8; COXPD6
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
product-image-AAA29950_ICC6.jpg ICC (Immunocytochemistry) (ICC staining MSH6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

MSH6, Polyclonal Antibody (Cat# AAA29950)

Full Name
MSH6 Antibody
Gene Names
MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
product-image-AAA22269_IP6.jpg IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of K-562 cells usingug hnRNP C Polyclonal Antibody. Western blot was performed from the immunoprecipitate using hnRNP C Polyclonal Antibody at a dilution of 1:1000.)

hnRNP C, Polyclonal Antibody (Cat# AAA22269)

Full Name
hnRNP C Polyclonal Antibody
Gene Names
HNRNPC; C1; C2; HNRNP; HNRPC; SNRPC
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
product-image-AAA19439_IF8.jpg IF (Immunofluorescence) (Figure 8. IF analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (AAA19439).TTC11/FIS1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TTC11/FIS1 Antibody (AAA19439) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

TTC11/FIS1, Polyclonal Antibody (Cat# AAA19439)

Full Name
Anti-TTC11/FIS1 Antibody Picoband
Gene Names
FIS1; TTC11; CGI-135
Reactivity
Human, Rat
Applications
WB, IHC, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19583_FCM6.jpg FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HeLa cells using anti-SLU7 antibody (AAA19583).Overlay histogram showing HeLa cells stained with AAA19583 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLU7 Antibody (AAA19583, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SLU7, Polyclonal Antibody (Cat# AAA19583)

Full Name
Anti-SLU7 Antibody Picoband
Gene Names
SLU7; 9G8; hSlu7
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
product-image-AAA19977_FCM7.png FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HepG2 cells using anti-IFT52 antibody (AAA19977).Overlay histogram showing HepG2 cells stained with AAA19977 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IFT52 Antibody (AAA19977, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IFT52, Polyclonal Antibody (Cat# AAA19977)

Full Name
Anti-IFT52 Antibody Picoband
Gene Names
IFT52; NGD2; NGD5; CGI-53; C20orf9; dJ1028D15.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA28373_IP6.jpg IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DGCR8 antibody . Western blot was performed from the immunoprecipitate using DGCR8 antibody at a dilition of 1:1000.)

DGCR8, Polyclonal Antibody (Cat# AAA28373)

Full Name
DGCR8 Rabbit pAb
Gene Names
DGCR8; Gy1; pasha; DGCRK6; C22orf12
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP
Purity
Affinity purification
Pricing
product-image-AAA28385_IF6.jpg IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using LLGL1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

LLGL1, Polyclonal Antibody (Cat# AAA28385)

Full Name
LLGL1 Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
product-image-AAA29156_IHC6.jpg IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IL-4, Polyclonal Antibody (Cat# AAA29156)

Full Name
IL-4 Polyclonal Antibody
Gene Names
IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
Reactivity
Human
Applications
WB, IHC-P
Pricing
product-image-AAA29677_IF6.jpg IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cell using PPP4C antibody. Blue: DAPI for nuclear staining.)

PPP4C, Polyclonal Antibody (Cat# AAA29677)

Full Name
PPP4C Antibody
Gene Names
PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
product-image-AAA28906_APP6.jpg Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Kv11.3, Polyclonal Antibody (Cat# AAA28906)

Full Name
Kv11.3 Polyclonal Antibody
Gene Names
KCNH7; ERG3; HERG3; Kv11.3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing

What are Polyclonal Antibodies?


Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).


Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.


Key Uses of Polyclonal Antibodies


  • Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

  • Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

  • ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

  • Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

  • Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.


Why Buy Polyclonal Antibodies from AAA Biotech?


1. Ideal for Various Applications


Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.


2. Rigorous Quality Control


All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.


3. Wide Assortment of Antibodies


Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.


4. Highly Purified


Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.


FAQ


1. How are polyclonal antibodies produced?


Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.


2. How do polyclonal antibodies differ from monoclonal antibodies?


Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.


3. How should I store polyclonal antibodies?


Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.


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