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FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of C6 cells using anti-BCL11B antibody (AAA19750).Overlay histogram showing C6 cells stained with AAA19750 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL11B Antibody (AAA19750, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
BCL11B, Polyclonal Antibody (Cat# AAA19750)
Full Name
Anti-BCL11B Antibody Picoband
Gene Names
BCL11B; ATL1; RIT1; CTIP2; IMD49; CTIP-2; ZNF856B; ATL1-beta; ATL1-alpha; ATL1-delta; ATL1-gamma; hRIT1-alpha
Reactivity
Human, Rat
Applications
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Purity
Immunogen affinity purified.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
SIRT2, Polyclonal Antibody (Cat# AAA31286)
Full Name
Phospho-SIRT2 (Ser368) Antibody
Gene Names
SIRT2; SIR2; SIR2L; SIR2L2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(SHMT/SHMT2 Antibody-Immunohistochemistry of paraffin-embedded rat spleen using SHMT2 antibody at dilution of 1:200 (200x lens).)
SHMT/SHMT2, Polyclonal Antibody (Cat# AAA21303)
Full Name
SHMT/SHMT2 Rabbit anti-Human Polyclonal Antibody
Reactivity
Mouse, Rat, Human
Applications
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Purity
Affinity purified
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody at dilution of 1:100 (40x lens).)
BAZ1B, Polyclonal Antibody (Cat# AAA28215)
Full Name
BAZ1B Polyclonal Antibody
Gene Names
BAZ1B; WSTF; WBSCR9; WBSCR10
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB)
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
CD31, Polyclonal Antibody (Cat# AAA28983)
Full Name
CD31 Polyclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Mouse
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
CacyBP, Polyclonal Antibody (Cat# AAA29239)
Full Name
CacyBP Polyclonal Antibody
Gene Names
CACYBP; SIP; GIG5; PNAS-107; S100A6BP; RP1-102G20.6
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Calnexin, Polyclonal Antibody (Cat# AAA28974)
Full Name
Calnexin Polyclonal Antibody
Gene Names
CANX; CNX; P90; IP90
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SAMHD1, Polyclonal Antibody (Cat# AAA31278)
Full Name
Phospho-SAMHD1 (Thr592) Antibody
Gene Names
SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Transfection Control
(Anti-GPR27 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)
GPR27, Polyclonal Antibody (Cat# AAA12335)
Full Name
Rabbit Polyclonal to Human GPR27
Gene Names
GPR27; SREB1
Reactivity
Gorilla, Human
Applications
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)
Purity
Immunoaffinity Purified
Pricing
IF (Immunofluorescence)
(Figure 8. IF analysis of GCLC using anti-GCLC antibody (AAA19759).GCLC was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-GCLC Antibody (AAA19759) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
GCLC, Polyclonal Antibody (Cat# AAA19759)
Full Name
Anti-GCLC Antibody Picoband
Gene Names
GCLC; GCL; GCS; GLCL; GLCLC
Reactivity
Human, Mouse, Rat
Applications
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 9. IF analysis of ATP5D/ATP5F1D using anti-ATP5D/ATP5F1D antibody (AAA20015).ATP5D/ATP5F1D was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ATP5D/ATP5F1D Antibody (AAA20015) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human Raji whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5D/ATP5F1D antigen affinity purified polyclonal antibody (#AAA20015) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATP5D/ATP5F1D at approximately 17 kDa. The expected band size for ATP5D/ATP5F1D is at 17 kDa.)
ATP5F1D, Polyclonal Antibody (Cat# AAA20015)
Full Name
Anti-ATP5F1D Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Troponin T-C, Polyclonal Antibody (Cat# AAA29233)
Full Name
Troponin T-C Polyclonal Antibody
Gene Names
TNNT2; CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
WB (Western Blot)
(Western Blot analysis of Hela cells using ETBR Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000)
ETBR, Polyclonal Antibody (Cat# AAA30769)
Full Name
ETBR Rabbit Polyclonal Antibody
Gene Names
EDNRB; ETB; ET-B; ETBR; ETRB; HSCR; WS4A; ABCDS; ET-BR; HSCR2
Reactivity
Human, Rat, Mouse
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PAK5/6, Polyclonal Antibody (Cat# AAA31281)
Full Name
Phospho-PAK5/6 (Ser602/Ser560) Antibody
Gene Names
PAK7; PAK5
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using Vimentin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
Vimentin, Polyclonal Antibody (Cat# AAA22322)
Full Name
Vimentin Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using CREBBP antibody at dilution of 1:100 (40x lens).)
CREBBP, Polyclonal Antibody (Cat# AAA28210)
Full Name
CREBBP Polyclonal Antibody
Gene Names
CREBBP; CBP; RSTS; KAT3A
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB)
Purity
Affinity Purification
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Caspase-9, Polyclonal Antibody (Cat# AAA28978)
Full Name
Caspase-9 Polyclonal Antibody
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Reactivity
Human
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
NTE, Polyclonal Antibody (Cat# AAA29234)
Full Name
NTE Polyclonal Antibody
Gene Names
PNPLA6; NTE; sws; SPG39; NTEMND; iPLA2delta
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
WB (Western Blot)
(Western Blot analysis of 3T3 cells using Nanos Homologue 1 (NANOS1) Polyclonal Antibody diluted at 1:1500. Secondary antibody was diluted at 1:20000)
Nanos Homologue 1 (NANOS1), Polyclonal Antibody (Cat# AAA30770)
Full Name
Nanos Homologue 1 (NANOS1) Rabbit Polyclonal Antibody
Gene Names
NANOS1; NOS1; SPGF12
Reactivity
Human, Rat, Mouse
Applications
Western Blot (WB), ELISA (EIA)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistchemistry)
(WAS/WASP Antibody-Immunohistochemistry of paraffin-embedded human esophagus using WAS antibody at dilution of 1:100 (200x lens).)
WAS/WASP, Polyclonal Antibody (Cat# AAA21299)
Full Name
WAS/WASP Rabbit anti-Human Polyclonal Antibody
Gene Names
WAS; THC; IMD2; SCNX; THC1; WASP
Reactivity
Human
Applications
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Purity
Affinity purified
IF (Immunofluorescence)
(Immunofluorescence analysis of rat-lung tissue. 1,CYCS Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
CYCS, Polyclonal Antibody (Cat# AAA30773)
Full Name
CYCS Rabbit Polyclonal Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
TOPBP1, Polyclonal Antibody (Cat# AAA31285)
Full Name
Phospho-TOPBP1 (Ser1138) Antibody
Gene Names
TOPBP1; TOP2BP1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
SQSTM1/p62, Polyclonal Antibody (Cat# AAA22326)
Full Name
(KO Validated) SQSTM1/p62 Polyclonal Antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
WB (Western Blot)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
CD284, Polyclonal Antibody (Cat# AAA28982)
Full Name
CD284 Polyclonal Antibody
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Reactivity
Human, Mouse
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
gp91-phox, Polyclonal Antibody (Cat# AAA29238)
Full Name
gp91-phox Polyclonal Antibody
Gene Names
CYBB; CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Apaf-1, Polyclonal Antibody (Cat# AAA29236)
Full Name
Apaf-1 Polyclonal Antibody
Gene Names
APAF1; CED4; APAF-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
OTUB1, Polyclonal Antibody (Cat# AAA31284)
Full Name
Phospho-OTUB1 (Ser16) Antibody
Gene Names
OTUB1; OTB1; OTU1; HSPC263
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Anti-DREG / GPR126 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
DREG / GPR126, Polyclonal Antibody (Cat# AAA12336)
Full Name
Rabbit Polyclonal to Human DREG / GPR126
Gene Names
GPR126; APG1; DREG; VIGR; PS1TP2
Reactivity
Gibbon, Gorilla, Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry (IHC - Paraffin)
Purity
Immunoaffinity Purified
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CTNNB1 Antibody (C-term) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
CTNNB1, Polyclonal Antibody (Cat# AAA28720)
Full Name
CTNNB1 Antibody (C-term)
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HepG2 cells using anti-GBF1 antibody (AAA19761).Overlay histogram showing HepG2 cells stained with AAA19761 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GBF1 Antibody (AAA19761, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
GBF1, Polyclonal Antibody (Cat# AAA19761)
Full Name
Anti-GBF1 Antibody Picoband
Gene Names
GBF1; ARF1GEF
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: CCDC109BSample Tissue: Mouse SP2/0 Whole Cell lysatesAntibody Dilution: 1ug/ml)
MCUB, Polyclonal Antibody (Cat# AAA23604)
Full Name
MCUB Antibody - middle region
Gene Names
Mcub; Ccdc109b; 9030408N13Rik
Reactivity
Tested Reactivity: Mouse
Predicted Reactivity: Mouse
Predicted Reactivity: Mouse
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of A549 cells using AAA26932 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
TRPM2, Polyclonal Antibody (Cat# AAA26932)
Full Name
TRPM2 Antibody
Gene Names
TRPM2; KNP3; EREG1; TRPC7; LTRPC2; NUDT9H; LTrpC-2; NUDT9L1
Reactivity
Human, mouse
Applications
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
>95%,Protein G purified
Pricing
IHC (Immunohistochemistry)
(ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ATP5B, Polyclonal Antibody (Cat# AAA28724)
Full Name
ATP5B Antibody (Center)
Gene Names
ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of RT4 cells using anti-LTA4H antibody (AAA19774).Overlay histogram showing RT4 cells stained with AAA19774 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LTA4H Antibody (AAA19774, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
LTA4H, Polyclonal Antibody (Cat# AAA19774)
Full Name
Anti-LTA4H Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.)
BHLH3, Polyclonal Antibody (Cat# AAA28734)
Full Name
BHLH3 Antibody (N-term)
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse
Applications
Flow Cytometry (FC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Cdc25B, Polyclonal Antibody (Cat# AAA28990)
Full Name
Cdc25B Polyclonal Antibody
Reactivity
Human
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
CD294, Polyclonal Antibody (Cat# AAA29246)
Full Name
CD294 Polyclonal Antibody
Gene Names
PTGDR2; DP2; DL1R; CD294; CRTH2; GPR44
Reactivity
Human, Mouse
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of human-lung tissue. 1,Ub Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Ub, Polyclonal Antibody (Cat# AAA30782)
Full Name
Ub Rabbit Polyclonal Antibody
Gene Names
UBA52; L40; CEP52; RPL40; HUBCEP52
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistchemistry)
(GADD45A/GADD45 Antibody-Immunohistochemistry of paraffin-embedded human esophagus using GADD45A antibody at dilution of 1:100 (400x lens).)
GADD45A/GADD45, Polyclonal Antibody (Cat# AAA21309)
Full Name
GADD45A/GADD45 Rabbit anti-Human Polyclonal Antibody
Gene Names
GADD45A; DDIT1; GADD45
Reactivity
Mouse, Human
Applications
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Purity
Affinity purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells usingug FUS Polyclonal Antibody. Western blot was performed from the immunoprecipitate using FUS Polyclonal Antibody at a dilution of 1:1000.)
FUS, Polyclonal Antibody (Cat# AAA22333)
Full Name
FUS Polyclonal Antibody
Gene Names
FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using TRPA1 antibody at dilution of 1:100 (40x lens).)
TRPA1, Polyclonal Antibody (Cat# AAA28221)
Full Name
TRPA1 Polyclonal Antibody
Gene Names
TRPA1; FEPS; ANKTM1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB)
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot analysis of HeLa cells using Acetyl-Ub (K27) Polyclonal Antibody. Secondary antibody was diluted at 1:20000)
Ub, Polyclonal Antibody (Cat# AAA30781)
Full Name
Ub (Acetyl-Lys27) Rabbit Polyclonal Antibody
Gene Names
UBA52; L40; CEP52; RPL40; HUBCEP52
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of U87 cells using anti-PKP2 antibody (AAA19770).Overlay histogram showing U87 cells stained with AAA19770 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKP2 Antibody (AAA19770, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PKP2, Polyclonal Antibody (Cat# AAA19770)
Full Name
Anti-PKP2 Antibody Picoband
Gene Names
PKP2; ARVD9
Reactivity
Human, Mouse, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(TAP2 Antibody-Immunohistochemistry of paraffin-embedded rat kidney using TAP2 antibody at dilution of 1:200 (x400 lens).)
TAP2, Polyclonal Antibody (Cat# AAA21306)
Full Name
TAP2 Rabbit anti-Human Polyclonal Antibody
Gene Names
TAP2; APT2; PSF2; ABC18; ABCB3; PSF-2; RING11; D6S217E
Reactivity
Mouse, Rat, Human
Applications
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Purity
Affinity purified
Pricing
WB (Western Blot)
(WB Suggested Anti-CTNNB1 Antibody Titration: 1.25ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)
CTNNB1, Polyclonal Antibody (Cat# AAA23610)
Full Name
CTNNB1 antibody - C-terminal region
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Zebrafish
Applications
Immunohistochemistry (IHC), Western Blot (WB)
Purity
Protein A purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
CD75, Polyclonal Antibody (Cat# AAA28986)
Full Name
CD75 Polyclonal Antibody
Gene Names
ST6GAL1; ST6N; SIAT1; ST6GalI
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of JK cells using anti-CD127/IL7R antibody (AAA19771).Overlay histogram showing JK cells stained with AAA19771 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD127/IL7R Antibody (AAA19771, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD127/IL7R, Polyclonal Antibody (Cat# AAA19771)
Full Name
Anti-CD127/IL7R Antibody Picoband
Gene Names
IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
Reactivity
Human
Applications
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(KPNA2/Importin Alpha 1 Antibody-Immunohistochemistry of paraffin-embedded human lung using KPNA2 antibody at dilution of 1:200 (200x lens).)
KPNA2/Importin Alpha 1, Polyclonal Antibody (Cat# AAA21307)
Full Name
KPNA2/Importin Alpha 1 Rabbit anti-Human Polyclonal Antibody
Gene Names
KPNA2; QIP2; RCH1; IPOA1; SRP1alpha
Reactivity
Mouse, Human
Applications
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)
Purity
Affinity purified
WB (Western Blot)
(Western Blot analysis of HeLa, HepG2 cells using Acetyl-NF?B-p65 (K314/K315) Polyclonal Antibody. Secondary antibody was diluted at 1:20000)
NFkappaB-p65, Polyclonal Antibody (Cat# AAA30779)
Full Name
NFkappaB-p65 (Acetyl-Lys314/Lys315) Rabbit Polyclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ID2, Polyclonal Antibody (Cat# AAA31291)
Full Name
Phospho-ID2 (Thr27) Antibody
Gene Names
ID2; GIG8; ID2A; ID2H; bHLHb26
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
