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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2350-2400 of 3176 product results
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)
GDF-6, Polyclonal Antibody (Cat# AAA29313)
Full Name
GDF-6 Polyclonal Antibody
Gene Names
GDF6; KFM; KFS; KFS1; KFSL; SGM1; BMP13; CDMP2; MCOP4; SCDO4; MCOPCB6
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
IHC (Immunohistchemistry)
(Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
KiSS-1R, Polyclonal Antibody (Cat# AAA29058)
Full Name
KiSS-1R Polyclonal Antibody
Gene Names
KISS1R; HH8; GPR54; AXOR12; KISS-1R; HOT7T175
Reactivity
Human
Applications
IF
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using YAP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)
YAP1, Polyclonal Antibody (Cat# AAA30592)
Full Name
YAP1 Rabbit Polyclonal Antibody
Gene Names
YAP1; YAP; YKI; YAP2; YAP65
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using ATG16L1 Rabbit pAb.)
ATG16L1, Polyclonal Antibody (Cat# AAA30598)
Full Name
ATG16L1 Rabbit Polyclonal Antibody
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
Ribosomal Protein L7, Polyclonal Antibody (Cat# AAA29111)
Full Name
Ribosomal Protein L7 Polyclonal Antibody
Gene Names
RPL7; L7; humL7-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
NFkappaB-p65, Polyclonal Antibody (Cat# AAA28852)
Full Name
NFkappaB-p65 (Acetyl Lys314/Lys315) Polyclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Human uterine cancer using C12orf10 Polyclonal Antibody at dilution of 1:100 (40x lens).)
C12orf10, Polyclonal Antibody (Cat# AAA22209)
Full Name
C12orf10 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using COPB2 antibody at dilution of 1:100 (40x lens).)
COPB2, Polyclonal Antibody (Cat# AAA28102)
Full Name
COPB2 Polyclonal Antibody
Gene Names
COPB2; beta'-COP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using HSPH1 antibody at dilution of 1:100 (40x lens).)
HSPH1, Polyclonal Antibody (Cat# AAA28100)
Full Name
HSPH1 Polyclonal Antibody
Gene Names
HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
IL-15, Polyclonal Antibody (Cat# AAA29382)
Full Name
IL-15 Polyclonal Antibody
Gene Names
IL15; IL-15
Reactivity
Human
Applications
IHC-P
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human thyroid cancer using CPSF6 Rabbit pAb.)
CPSF6, Polyclonal Antibody (Cat# AAA30676)
Full Name
CPSF6 Rabbit Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of U2OS cells using KU70 Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.)
KU70, Polyclonal Antibody (Cat# AAA30688)
Full Name
KU70 Rabbit Polyclonal Antibody
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot analysis of FKBP4 expression in transfected 293T cell line by FKBP4 polyclonal antibody. Lane 1: FKBP4 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)
FKBP4, Polyclonal Antibody (Cat# AAA26868)
Full Name
FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) (Biot
Gene Names
FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
Reactivity
Human, Mouse
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
P2X3, Polyclonal Antibody (Cat# AAA29180)
Full Name
P2X3 Polyclonal Antibody
Gene Names
P2RX3; P2X3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 antibody. Western blot was performed from the immunoprecipitate using CDK4 antibody at a dilution of 1:1000.)
CDK4, Polyclonal Antibody (Cat# AAA28411)
Full Name
[KO Validated] CDK4 Rabbit pAb
Reactivity
Human, Mouse
Applications
WB, IHC, IP, IF, ICC
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of PC-3 cells using anti-HES5 antibody (AAA19545).Overlay histogram showing PC-3 cells stained with AAA19545 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HES5 Antibody (AAA19545, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HES5, Polyclonal Antibody (Cat# AAA19545)
Full Name
Anti-HES5 Antibody Picoband
Gene Names
HES5; bHLHb38
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot;Sample: Porcine Cerebrum lysatePrimary Ab: 1ug/ml Rabbit Anti-Porcine VEGFR2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Vascular Endothelial Growth Factor Receptor 2, Polyclonal Antibody (Cat# AAA20930)
Full Name
Polyclonal Antibody to Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)
Reactivity
Pig, Human, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PEX10 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with AAA11049 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
PEX10, Polyclonal Antibody (Cat# AAA11049)
Full Name
PEX10 (CT) Antibody
Gene Names
PEX10; NALD; PBD6A; PBD6B; RNF69
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IF
Purity
PEX10 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
EEF1A2, Polyclonal Antibody (Cat# AAA31289)
Full Name
Phospho-EEF1A2 (Ser358) Antibody
Gene Names
EEF1A2; HS1; STN; EF1A; STNL; EEF1AL; EF-1-alpha-2
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(WB Suggested Anti-PTX3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate)
PTX3, Polyclonal Antibody (Cat# AAA23569)
Full Name
PTX3 antibody - N-terminal region
Gene Names
PTX3; TSG-14; TNFAIP5
Reactivity
Cow, Guinea Pig, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using HPSE Antibody at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG)
heparanase, Polyclonal Antibody (Cat# AAA15907)
Full Name
Rabbit anti-human heparanase polyclonal Antibody
Gene Names
HPSE; HPA; HPA1; HPR1; HSE1; HPSE1
Reactivity
Human, Mouse; Other species are not tested. Please decide the specificity by homology.
Applications
EIA, IHC
Purity
Antigen Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-FOXM1 Antibody Titration: 1 ug/mlPositive Control: Jurkat cell lysate)
FOXM1, Polyclonal Antibody (Cat# AAA23606)
Full Name
FOXM1 antibody - middle region
Gene Names
FOXM1; MPP2; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1A; FOXM1B; FOXM1C; HFH-11; TRIDENT; MPHOSPH2
Reactivity
Dog, Guinea Pig, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-FOXA1 antibody Titration: 1 ug/mLSample Type: Human liver)
FOXA1, Polyclonal Antibody (Cat# AAA23415)
Full Name
FOXA1 antibody - C-terminal region
Gene Names
FOXA1; HNF3A; TCF3A
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rat, Yeast, Zebrafish
Applications
WB, IHC
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A431 cells using anti-BIK antibody (AAA11666).Overlay histogram showing A431 cells stained with AAA11666 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BIK Antibody (AAA11666,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Bik, Polyclonal Antibody (Cat# AAA11666)
Full Name
Anti-Bik Antibody
Gene Names
BIK; BP4; NBK; BIP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p27 Kip1, Polyclonal Antibody (Cat# AAA31387)
Full Name
Phospho-p27 Kip1 (Thr157) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Raw264.7 cells using CD44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
CD44, Polyclonal Antibody (Cat# AAA28336)
Full Name
CD44 Rabbit pAb
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
Rag A/B, Polyclonal Antibody (Cat# AAA29106)
Full Name
Rag A/B Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistchemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
HSP 75, Polyclonal Antibody (Cat# AAA29376)
Full Name
HSP 75 Polyclonal Antibody
Gene Names
TRAP1; HSP75; HSP 75; HSP90L; TRAP-1
Reactivity
Human
Applications
IHC-P
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)
Tau, Polyclonal Antibody (Cat# AAA29353)
Full Name
Tau Polyclonal Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human lung using TXN Polyclonal Antibody at dilution of 1:100 (40x lens).)
TXN, Polyclonal Antibody (Cat# AAA22183)
Full Name
TXN Polyclonal Antibody
Gene Names
TXN; TRX; TRDX; TRX1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
WB (Western Blot)
(Figure 7. Western blot analysis of PLS1 using anti-PLS1 antibody (AAA19893).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: mouse small intestine tissue lysates,Lane 6: rat stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLS1 antigen affinity purified polyclonal antibody (AAA19893) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for PLS1 at approximately 70 kDa. The expected band size for PLS1 is at 70 kDa.)
PLS1, Polyclonal Antibody (Cat# AAA19893)
Full Name
Anti-PLS1 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cell using HNMT antibody. Blue: DAPI for nuclear staining.)
HNMT, Polyclonal Antibody (Cat# AAA29651)
Full Name
HNMT Antibody
Gene Names
HNMT; HMT; HNMT-S1; HNMT-S2
Reactivity
Human
Applications
WB, IHC, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
IF (Immunofluorescence)
(Immunofluorescence of PPARGC1A in rat heart tissue with PPARGC1A antibody at 20 μg/mL.)
PPARGC1A, Polyclonal Antibody (Cat# AAA10967)
Full Name
PPARGC1A Antibody
Gene Names
PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC-p, IF
Purity
PPARGC1A antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Detection of mouse RPA32 by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody (AAA23770 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
RPA32, Polyclonal Antibody (Cat# AAA23770)
Full Name
Rabbit anti-RPA32 Antibody, Affinity Purified
Gene Names
RPA2; REPA2; RPA32
Reactivity
Human, Mouse
Applications
WB, IP, IHC, FC/FACS
Purity
Antigen Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using CSK Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
CSK, Polyclonal Antibody (Cat# AAA22262)
Full Name
CSK Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Figure 9. IF analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
SARS2, Polyclonal Antibody (Cat# AAA19598)
Full Name
Anti-SARS2 Antibody Picoband
Gene Names
SARS2; SYS; SARS; SERS; SARSM; SerRS; SerRSmt; mtSerRS
Reactivity
Human
Applications
WB, IHC, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26903)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using SQSTM1/p62 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
SQSTM1/p62, Polyclonal Antibody (Cat# AAA22326)
Full Name
(KO Validated) SQSTM1/p62 Polyclonal Antibody
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
CD31, Polyclonal Antibody (Cat# AAA28983)
Full Name
CD31 Polyclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Mouse
Applications
WB, IHC-P, IF
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
ChemR23, Polyclonal Antibody (Cat# AAA28994)
Full Name
ChemR23 Polyclonal Antibody
Gene Names
CMKLR1; DEZ; RVER1; ChemR23; CHEMERINR
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
eIF4B, Polyclonal Antibody (Cat# AAA31302)
Full Name
Phospho-eIF4B (Ser504) Antibody
Gene Names
EIF4B; EIF-4B; PRO1843
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(WB Suggested Anti-STAT6 Antibody Titration: 1 ug/mlPositive Control: Hela cell lysateSTAT6 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)
STAT6, Polyclonal Antibody (Cat# AAA23382)
Full Name
STAT6 antibody - C-terminal region
Gene Names
STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
WB, IHC
Purity
Affinity Purified
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
MCM2, Polyclonal Antibody (Cat# AAA31328)
Full Name
Phospho-MCM2 (Ser108) Antibody
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
SNAI 1, Polyclonal Antibody (Cat# AAA29120)
Full Name
SNAI 1 Polyclonal Antibody
Gene Names
SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
Reactivity
Human, Mouse, Monkey
Applications
WB, IHC-P, IF
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of PC-3 cells using anti-POC1A antibody (AAA19908).Overlay histogram showing PC-3 cells stained with AAA19908 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POC1A Antibody (AAA19908, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
POC1A, Polyclonal Antibody (Cat# AAA19908)
Full Name
Anti-POC1A Antibody Picoband
Gene Names
POC1A; PIX2; SOFT; WDR51A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 10. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
COX IV, Polyclonal Antibody (Cat# AAA19173)
Full Name
Anti-COX IV Picoband Antibody
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
WB (Western Blot)
(WB Suggested Anti-FER1L3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate.MYOF is strongly supported by BioGPS gene expression data to be expressed in MCF7)
FER1L3, Polyclonal Antibody (Cat# AAA23526)
Full Name
FER1L3 antibody - C-terminal region
Gene Names
MYOF; FER1L3
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
TALL-1, Polyclonal Antibody (Cat# AAA29164)
Full Name
TALL-1 Polyclonal Antibody
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
Reactivity
Human, Mouse
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse breast cells using PMAIP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)
PMAIP1, Polyclonal Antibody (Cat# AAA30715)
Full Name
PMAIP1 Rabbit Polyclonal Antibody
Gene Names
PMAIP1; APR; NOXA
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A549 cells using anti-DR6/TNFRSF21 antibody (AAA19292).Overlay histogram showing A549 cells stained with AAA19292 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DR6/TNFRSF21 Antibody (AAA19292, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DR6/TNFRSF21, Polyclonal Antibody (Cat# AAA19292)
Full Name
Anti-DR6/TNFRSF21 Antibody
Gene Names
TNFRSF21; DR6; CD358; BM-018
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.