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WB (Western Blot) (Western Blot analysis of FKBP4 expression in transfected 293T cell line by FKBP4 polyclonal antibody. Lane 1: FKBP4 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)

FKBP4, Polyclonal Antibody (Cat# AAA26866)

Full Name

FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) (AP)

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human, Mouse

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

CUL-4B, Polyclonal Antibody (Cat# AAA29170)

Full Name

CUL-4B Polyclonal Antibody

Gene Names

CUL4B; SFM2; MRXSC; CUL-4B; MRXHF2; MRXS15

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Western blot analysis of extracts of Mouse spleen, using CD163 antibody.)

CD163, Polyclonal Antibody (Cat# AAA30706)

Full Name

CD163 Rabbit Polyclonal Antibody

Gene Names

CD163; M130; MM130

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of SiHa cells using anti-SF3A2 antibody (AAA19955).Overlay histogram showing SiHa cells stained with AAA19955 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SF3A2 Antibody (AAA19955, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of SF3A2 using anti-SF3A2 antibody (AAA19955) and anti-Beta Tubulin antibody (M01857-3).SF3A2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SF3A2 Antibody (AAA19955) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SF3A2 Antibody (AAA19955) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SF3A2 Antibody (AAA19955) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SF3A2 Antibody (AAA19955) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SF3A2 Antibody (AAA19955) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SF3A2 Antibody (AAA19955) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SF3A2 Antibody (AAA19955) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: rat thymus tissue lysates,Lane 6: rat ovary tissue lysates,Lane 7: mouse thymus tissue lysates,Lane 8: mouse ovary tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A2 antigen affinity purified polyclonal antibody (#AAA19955) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (60,66 kDa.)

SF3A2, Polyclonal Antibody (Cat# AAA19955)

Full Name

Anti-SF3A2 Antibody Picoband

Gene Names

SF3A2; PRP11; SAP62; PRPF11; SF3a66

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot analysis of FKBP4 expression in transfected 293T cell line by FKBP4 polyclonal antibody. Lane 1: FKBP4 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)

FKBP4, Polyclonal Antibody (Cat# AAA26867)

Full Name

FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) APC

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human, Mouse

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of MG-63 cells with Kv4.3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody)

KV4.3, Polyclonal Antibody (Cat# AAA29939)

Full Name

KV4.3 Antibody

Gene Names

KCND3; KV4.3; SCA19; SCA22; KCND3L; KCND3S; KSHIVB

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEL cells using anti-STXBP6 antibody (AAA19956).Overlay histogram showing HEL cells stained with AAA19956 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STXBP6 Antibody (AAA19956, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of STXBP6 using anti-STXBP6 antibody (AAA19956).STXBP6 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human Ramos whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP6 antigen affinity purified polyclonal antibody (#AAA19956) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STXBP6 at approximately 26 kDa. The expected band size for STXBP6 is at 24 kDa.)

STXBP6, Polyclonal Antibody (Cat# AAA19956)

Full Name

Anti-STXBP6 Antibody Picoband

Gene Names

STXBP6; amisyn; HSPC156

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

TF, Polyclonal Antibody (Cat# AAA29172)

Full Name

TF Polyclonal Antibody

Gene Names

F3; TF; TFA; CD142

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using CSK Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

CSK, Polyclonal Antibody (Cat# AAA22262)

Full Name

CSK Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

CRP, Polyclonal Antibody (Cat# AAA29174)

Full Name

CRP Polyclonal Antibody

Gene Names

CRP; PTX1

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cell using KPNA2 antibody. Blue: DAPI for nuclear staining.)

KPNA2, Polyclonal Antibody (Cat# AAA29686)

Full Name

KPNA2 Antibody

Gene Names

KPNA2; QIP2; RCH1; IPOA1; SRP1alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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WB (Western Blot) (Western Blot analysis of FKBP4 expression in transfected 293T cell line by FKBP4 polyclonal antibody. Lane 1: FKBP4 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)

FKBP4, Polyclonal Antibody (Cat# AAA26869)

Full Name

FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) (FITC

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human, Mouse

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using GLUD2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

GLUD2, Polyclonal Antibody (Cat# AAA28149)

Full Name

GLUD2 Polyclonal Antibody

Gene Names

GLUD2; GDH2; GLUDP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western blot analysis of extracts of mouse liver, using TRPA1 at 1:1000 dilution.)

TRPA1, Polyclonal Antibody (Cat# AAA30709)

Full Name

TRPA1 Rabbit Polyclonal Antibody

Gene Names

TRPA1; FEPS; ANKTM1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26874)

Full Name

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (Biotin)

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Mouse, Human

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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NASP, Polyclonal Antibody (Cat# AAA28154)

Full Name

NASP

Gene Names

NASP; FLB7527; PRO1999

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Pricing

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WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Chk2, Polyclonal Antibody (Cat# AAA28922)

Full Name

Chk2 (phospho Thr68) Polyclonal Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of RT4 cells using anti-SSH3 antibody (AAA19963).Overlay histogram showing RT4 cells stained with AAA19963 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSH3 Antibody (AAA19963, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of SSH3 using anti-SSH3 antibody (AAA19963) and anti-Beta Tubulin antibody (M01857-3).SSH3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSH3 Antibody (AAA19963) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSH3 Antibody (AAA19963) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSH3 Antibody (AAA19963) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293Twhole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3 antigen affinity purified polyclonal antibody (#AAA19963) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SSH3 at approximately 95 kDa. The expected band size for SSH3 is at 73 kDa.)

SSH3, Polyclonal Antibody (Cat# AAA19963)

Full Name

Anti-SSH3 Antibody Picoband

Gene Names

SSH3; SSH3L

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-ATG5 Antibody Titration: 1 ug/mlPositive Control: HepG2 cell lysate)

ATG5, Polyclonal Antibody (Cat# AAA23551)

Full Name

ATG5 antibody - C-terminal region

Gene Names

ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using [KO Validated] Peroxiredoxin 1/PAG Rabbit pAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.)

Peroxiredoxin 1/PAG, Polyclonal Antibody (Cat# AAA28415)

Full Name

[KD Validated] Peroxiredoxin 1/PAG Rabbit pAb

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Lipocalin-1, Polyclonal Antibody (Cat# AAA29183)

Full Name

Lipocalin-1 Polyclonal Antibody

Gene Names

LCN1; TP; TLC; PMFA; VEGP

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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CDKN1A, Polyclonal Antibody (Cat# AAA10750)

Full Name

CDKN1A

Gene Names

CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Reactivity

Human, mouse, rat

Applications

ELISA, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP).

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using PDE4A Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.)

PDE4A, Polyclonal Antibody (Cat# AAA22270)

Full Name

PDE4A Polyclonal Antibody

Gene Names

PDE4A; PDE4; DPDE2; PDE46

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-SQSTM1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Pancreas)

SQSTM1, Polyclonal Antibody (Cat# AAA23550)

Full Name

SQSTM1 antibody - middle region

Gene Names

SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3

Reactivity

Tested Species Reactivity: Human, Mouse
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using CORO1A Rabbit pAb.)

CORO1A, Polyclonal Antibody (Cat# AAA30712)

Full Name

CORO1A Rabbit Polyclonal Antibody

Gene Names

CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-ACTR1B Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysateACTR1B is supported by BioGPS gene expression data to be expressed in HepG2)

ACTR1B, Polyclonal Antibody (Cat# AAA23545)

Full Name

ACTR1B antibody - C-terminal region

Gene Names

ACTR1B; PC3; ARP1B; CTRN2

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of HepG2 cells using anti-SGK493/PKDCC antibody (AAA19960).Overlay histogram showing HepG2 cells stained with AAA19960 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGK493/PKDCC Antibody (AAA19960, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of SGK493/PKDCC using anti-SGK493/PKDCC antibody (AAA19960).SGK493/PKDCC was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SGK493/PKDCC Antibody (AAA19960) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SGK493/PKDCC Antibody (AAA19960) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SGK493/PKDCC Antibody (AAA19960) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SGK493/PKDCC Antibody (AAA19960) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SGK493/PKDCC Antibody (AAA19960) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SGK493/PKDCC Antibody (AAA19960) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SGK493/PKDCC Antibody (AAA19960) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGK493/PKDCC antigen affinity purified polyclonal antibody (#AAA19960) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SGK493/PKDCC at approximately 54 kDa. The expected band size for SGK493/PKDCC is at 54 kDa.)

SGK493/PKDCC, Polyclonal Antibody (Cat# AAA19960)

Full Name

Anti-SGK493/PKDCC Antibody Picoband

Gene Names

PKDCC; Vlk; SGK493

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26872)

Full Name

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (AP)

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Mouse, Human

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using Caspase-3 p17 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Caspase-3 p17, Polyclonal Antibody (Cat# AAA28408)

Full Name

[KO Validated] Caspase-3 p17 Rabbit pAb

Gene Names

CARTPT; CART

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

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TRAF3IP3, Polyclonal Antibody (Cat# AAA22007)

Full Name

TRAF3IP3 Polyclonal Antibody

Gene Names

TRAF3IP3; T3JAM

Reactivity

Human, Mouse

Applications

ELISA (EIA), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Gelsolin, Polyclonal Antibody (Cat# AAA22263)

Full Name

Gelsolin Polyclonal Antibody

Gene Names

GSN; ADF; AGEL

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with ApoER2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ApoER2, Polyclonal Antibody (Cat# AAA29943)

Full Name

ApoER2 Antibody

Gene Names

LRP8; MCI1; LRP-8; APOER2; HSZ75190

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC)

Purity

Protein affinity purified

Pricing

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WB (Western Blot) (WB Suggested Anti-CAMKK2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysateThere is BioGPS gene expression data showing that CAMKK2 is expressed in 721_B)

CAMKK2, Polyclonal Antibody (Cat# AAA23549)

Full Name

CAMKK2 antibody - N-terminal region

Gene Names

CAMKK2; CAMKK; CAMKKB

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Smad1, Polyclonal Antibody (Cat# AAA28413)

Full Name

[KO Validated] Smad1 Rabbit pAb

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using S100A9 Rabbit pAb.)

S100A9, Polyclonal Antibody (Cat# AAA30717)

Full Name

S100A9 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26878)

Full Name

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 490)

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Mouse, Human

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 using Heme Oxygenase 1 (HO-1/HMOX1) antibody at dilution of 1 : 50 (40x lens). Blue: DAPI for nuclear staining.)

Heme Oxygenase 1 (HO-1/HMOX1), Polyclonal Antibody (Cat# AAA28414)

Full Name

[KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb

Gene Names

NR1D2; RVR; BD73; EAR-1R

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

ERK 1/2, Polyclonal Antibody (Cat# AAA28926)

Full Name

ERK 1/2 (phospho Tyr204) Polyclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Keap1, Polyclonal Antibody (Cat# AAA29182)

Full Name

Keap1 Polyclonal Antibody

Gene Names

KEAP1; INrf2; KLHL19

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using ATF2 antibody. Blue: DAPI for nuclear staining.)

ATF2, Polyclonal Antibody (Cat# AAA10751)

Full Name

ATF2 Polyclonal Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U937 cells using anti-Syntaxin 18/STX18 antibody (AAA19967).Overlay histogram showing U937 cells stained with AAA19967 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (AAA19967).Syntaxin 18/STX18 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 18/STX18 antigen affinity purified polyclonal antibody (#AAA19967) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Syntaxin 18/STX18 at approximately 39 kDa. The expected band size for Syntaxin 18/STX18 is at 39 kDa.)

Syntaxin 18/STX18, Polyclonal Antibody (Cat# AAA19967)

Full Name

Anti-Syntaxin 18/STX18 Antibody Picoband

Gene Names

STX18; Ufe1

Reactivity

Human

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using LLGL1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

LLGL1, Polyclonal Antibody (Cat# AAA22271)

Full Name

LLGL1 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (CRFR2 Antibody (D35) flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

CRHR2 / CRF2 Receptor, Polyclonal Antibody (Cat# AAA21272)

Full Name

Anti-CRHR2 / CRF2 Receptor Antibody (aa10-39) IHC-plus

Gene Names

CRHR2; CRF2; CRFR2; CRF-RB; HM-CRF

Reactivity

Human

Applications

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Protein A purified

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26876)

Full Name

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (HRP)

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Mouse, Human

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug N-Cadherin antibody. Western blot was performed from the immunoprecipitate using N-Cadherin antibody at a dilution of 1:1000.)

N-Cadherin, Polyclonal Antibody (Cat# AAA28412)

Full Name

[KO Validated] N-Cadherin Rabbit pAb

Gene Names

LRRC59; p34; PRO1855

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human testis tissue reacted with PARK2 (Parkin) antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)