Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using DYNLL1 Rabbit pAb (AAA28275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using DYNLL1 Rabbit pAb (AAA28275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using DYNLL1 Rabbit pAb (AAA28275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using DYNLL1 Rabbit pAb (AAA28275) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using DYNLL1 Rabbit pAb (AAA28275) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using DYNLL1 Rabbit pAb (AAA28275) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DYNLL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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DYNLL1, Polyclonal Antibody (Cat# AAA28275)

Full Name

DYNLL1 Polyclonal Antibody

Gene Names

DYNLL1; LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Histone H2A (acetyl K5) in lysates of A431 cells(TSA 1M, 18 hr), using Histone H2A (acetyl K5) Antibody(AF4354).)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Acetyl-Histone H2A (Lys5) Antibody.Lane 1: H2O2 treated MDA-MB-231 cells, blocked with antigen-specific peptides,Lane 2: H2O2 treated MDA-MB-231 cells,Lane 3: Heat-shock treated RAW264.7 cells.7,Lane 4: UV treated MCF7 cells.)

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Histone H2A, Polyclonal Antibody (Cat# AAA31347)

Full Name

Acetyl-Histone H2A (Lys5) Antibody

Gene Names

HIST1H2AB; H2A/m; H2AFM

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of A-431 using Cortactin antibody at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat colon using CTSC antibody (Please inquire) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ESRRA antibody (AAA28272) at dilution of 1:200 (40xlens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ESRRA antibody (AAA28272) at dilution of 1:200 (40xlens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterus using ESRRA antibody (AAA28272) at dilution of 1:200 (40xlens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ESRRA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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ESRRA, Polyclonal Antibody (Cat# AAA28272)

Full Name

ESRRA Polyclonal Antibody

Gene Names

ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using S100A8 antibody at dilution of 1:200 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human skin using S100A8 antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using S100A8 antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using S100A8 antibody at dilution of 1:200 .)

IF (Immunofluorescence) (Confocal immunofluorescence analysis of U-2 OS cells using S100A8 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of human skin cancer using S100A8 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using S100A8 antibody at 1:1000 dilution.)

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S100A8, Polyclonal Antibody (Cat# AAA30576)

Full Name

S100A8 Polyclonal Antibody

Gene Names

S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Western blot analysis of p70 S6 Kinase expression in mouse muscle tissue lysates, The lane on the right is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA31088 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA31088 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (AAA31088 at 1/100 staining human brain tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 55 degree C)

IF (Immunofluorescence) (AAA31088 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of p70 S6 Kinase expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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p70 S6 Kinase, Polyclonal Antibody (Cat# AAA31088)

Full Name

p70 S6 Kinase Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

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IP (Immunoprecipitation) (IP/WB analysis of mouse brain, rat brain membrane lysate and mouse brain membrane lysate, were resolved by electrophoresis, transferred to PVDF membrane and probed using AAA14705 (1 :500). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein AMYLOID OLIGOMER (-80kD).)

IP (Immunoprecipitation) (IP/WB analysis of mouse brain lysate, was resolved by electrophoresis, transferred to PVDF membrane and probed using AAA14705 (1:500).Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein AMYLOID OLIGOMER (~80kD).)

IF (Immunofluorescence) (Immunolocalization of oligomeric beta amyloid (red) in human Alzheimer's Disease brain. Astrocytes (arrow) are stained with AAA14705 (green).)

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b-Amyloid, Oligomer A11, Polyclonal Antibody (Cat# AAA14705)

Full Name

b-Amyloid, Oligomer A11

Reactivity

Human, Mouse, Rat and Eukaryote

Applications

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Serum

Pricing

$1,065/0.1 mL $2,065/2x0.1 mL $3,010/3x0.1 mL $3,900/4x0.1 mL $4,575/5x0.1 mL

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IHC (Immunohistochemistry) (CDH1/E Cadherin Antibody-Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistchemistry) (CDH1/E Cadherin Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CDH1/E Cadherin Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CDH1/E Cadherin Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (CDH1/E Cadherin Antibody-Immunoprecipitating CDH1 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CDH1 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CDH1 Antibody (6ug) + HEK293 whole cell lysate (500ug) Lane 3: HEK293 whole cell lysate (20ug))

WB (Western Blot) (CDH1/E Cadherin Antibody-Western Blot Positive WB detected in: 293 whole cell lysate All Lanes: CDH1 antibody at 2.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 98, 91 KDa Observed band size: 125 KDa)

ICC (Immunocytochemistry) (CDH1/E Cadherin Antibody-Immunofluorescence staining of HepG2 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

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CDH1/E Cadherin, Polyclonal Antibody (Cat# AAA21361)

Full Name

CDH1/E Cadherin Rabbit anti-Human Polyclonal Antibody

Gene Names

CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Protein G purified

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

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HoxA11/D11, Polyclonal Antibody (Cat# AAA29041)

Full Name

HoxA11/D11 Polyclonal Antibody

Gene Names

HOXA11; HOX1; HOX1I

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using BRIX1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using BRIX1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using BRIX1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using BRIX1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung using BRIX1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BRIX1 antibody.)

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BRIX1, Polyclonal Antibody (Cat# AAA29809)

Full Name

BRIX1 Polyclonal Antibody

Gene Names

BRIX1; BRIX; BXDC2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (AAA31089 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31089 at 1/100 staining human brain cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA31089 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Akt expression in Serum treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of extracts of various tissue, using akt antibody.)

WB (Western Blot) (Western blot analysis of Akt Antibody expression in Various cell/tissue lysates.)

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AKT, Polyclonal Antibody (Cat# AAA31089)

Full Name

AKT Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunoflurescene (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

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Application Data

Application Data

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anti-Human IgM, Polyclonal Antibody (Cat# AAA14450)

Full Name

Affinity Purified Goat anti-Human IgM

Reactivity

Human

Purity

Antigen Affinity Purified

Pricing

$210/1 mg $400/2x1 mg $575/3x1 mg $710/5x1 mg $740/4x1 mg

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IHC (Immunohistchemistry) (S100A6/Calcyclin Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (S100A6/Calcyclin Antibody-Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (S100A6/Calcyclin Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (S100A6/Calcyclin Antibody-Western blot of recombinant S100A6. This image was taken for the unconjugated form of this product. Other forms have not been tested.)

WB (Western Blot) (S100A6/Calcyclin Antibody-Western Blot; Sample: Lane1: Human Hela Cells; Lane2: Human A549 Cells; Lane3: Human MCF7 Cells.)

WB (Western Blot) (S100A6/Calcyclin Antibody-Knockout Varification: Lane 1: Wild-type Hela cell lysate; Lane 2: S100A6 knockout Hela cell lysate; Predicted MW: 10kDa ; Observed MW: 10kDa; Primary Ab: 4ug/ml Rabbit Anti-Human S100A6 Antibody; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

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S100A6/Calcyclin, Polyclonal Antibody (Cat# AAA21367)

Full Name

S100A6/Calcyclin Rabbit anti-Human Polyclonal (aa1-90) Antibody

Gene Names

S100A6; 2A9; PRA; 5B10; CABP; CACY

Reactivity

Human

Applications

Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

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IHC (Immunohistchemistry-Paraffin) (Immunochemical staining of human YBX1 in rat stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human YBX1 in rat kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IP (Immunoprecipitation) (Immunochemical staining of human YBX1 in mouse stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human YBX1 in mouse kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human YBX1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human YBX1 in human breast carcinoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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YBX1/YB-1, Polyclonal Antibody (Cat# AAA27767)

Full Name

YBX1/YB-1 Antibody, Rabbit PAb, Antigen Affinity Purified

Gene Names

YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ZNF433 Polyclonal Antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ZNF433 Polyclonal Antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ZNF433 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ZNF433 antibody.)

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ZNF433, Polyclonal Antibody (Cat# AAA29815)

Full Name

ZNF433 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (AAA31095 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA31095 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts of various smaple, using Histone H3 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various smaple, using Histone H3 antibody.)

WB (Western Blot) (Western blot analysis of extracts of Rat spleen tissue, using Histone H3 antibody.)

WB (Western Blot) (Western blot analysis of Histone H3 expression in TSA treated RAW264.7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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Histone H3, Polyclonal Antibody (Cat# AAA31095)

Full Name

Histone H3 Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

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IL-3Ralpha, Polyclonal Antibody (Cat# AAA29300)

Full Name

IL-3Ralpha Polyclonal Antibody

Gene Names

IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ZNF177 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ZNF177 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ZNF177 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using ZNF177 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ZNF177 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ZNF177 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using ZNF177 antibody.)

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ZNF177, Polyclonal Antibody (Cat# AAA29812)

Full Name

ZNF177 Polyclonal Antibody

Gene Names

ZNF177; PIGX

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using ELP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using ELP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using ELP3 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using ELP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using ELP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using ELP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using ELP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ELP3 antibody.)

WB (Western Blot) (Western blot analysis of extracts of Mouse kidney, using ELP3 antibody.)

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ELP3, Polyclonal Antibody (Cat# AAA30580)

Full Name

ELP3 Polyclonal Antibody

Gene Names

ELP3; KAT9

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Hela cells using anti-NUCKS1 antibody (AAA19830).Overlay histogram showing Hela cells stained with AAA19830 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUCKS1 Antibody (AAA19830, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of NUCKS1 using anti-NUCKS1 antibody (AAA19830).NUCKS1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-NUCKS1 Antibody (AAA19830) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 8. IF analysis of NUCKS1 using anti-NUCKS1 antibody (AAA19830).NUCKS1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-NUCKS1 Antibody (AAA19830) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of NUCKS1 using anti-NUCKS1 antibody (AAA19830).NUCKS1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUCKS1 Antibody (AAA19830) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUCKS1 Antibody (AAA19830) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUCKS1 Antibody (AAA19830) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUCKS1 Antibody (AAA19830) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUCKS1 Antibody (AAA19830) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUCKS1 Antibody (AAA19830) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SH-SY5Y whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUCKS1 antigen affinity purified polyclonal antibody (#AAA19830) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NUCKS1 at approximately 20 kDa. The expected band size for NUCKS1 is at 27 kDa.)

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NUCKS1, Polyclonal Antibody (Cat# AAA19830)

Full Name

Anti-NUCKS1 Antibody Picoband

Gene Names

NUCKS1; JC7; NUCKS

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

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PSGL-1, Polyclonal Antibody (Cat# AAA29302)

Full Name

PSGL-1 Polyclonal Antibody

Gene Names

SELPLG; CLA; CD162; PSGL1; PSGL-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (MDH/MDH2 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (MDH/MDH2 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (MDH/MDH2 Antibody-Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (MDH/MDH2 Antibody-Antibody (0.03ug/ml) staining of Human Heart (A) and Liver (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) (MDH/MDH2 Antibody-Antibody (0.03ug/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) (MDH/MDH2 Antibody-Goat anti-MDH2 (aa221-232) Antibody (0.03ug/ml) staining of Human Heart (A) and Liver (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)

WB (Western Blot) (MDH/MDH2 Antibody-Goat anti-MDH2 (aa221-232) Antibody (0.03ug/ml) staining of HeLa (A), HepG2 (B) and K562 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)

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MDH/MDH2, Polyclonal Antibody (Cat# AAA21369)

Full Name

MDH/MDH2 Goat anti-Human Polyclonal (aa221-232) Antibody

Gene Names

MDH2; MDH; MOR1; M-MDH; MGC:3559

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

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IHC (Immunohistchemistry-Paraffin) (Immunochemical staining of human PCNA in human small intestine with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human PCNA in human skin with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human PCNA in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of rat A in rat skin with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of rat A in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human PCNA in human ovarian cancer with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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PCNA, Polyclonal Antibody (Cat# AAA27769)

Full Name

Anti-PCNA Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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FCM (Flow Cytometry) (SEPT9 Antibody (A555) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (SEPT9 Antibody (A555) immunohistochemistry analysis in formalin fixed and paraffin embedded kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEPT9 Antibody (A555) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (The anti-SEPT9 Pab is used in Western blot to detect SEPT9 in Jurkat cell lysate.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of lysates from human kidney and liver tissue lysate (from left to right), using SEPT9 Antibody (C-term). AAA28793 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from A431, Hela cell line (from left to right), using SEPT9 Antibody (C-term). AAA28793 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

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SEPT9, Polyclonal Antibody (Cat# AAA28793)

Full Name

SEPT9 Antibody (C-term)

Gene Names

SEPT9; MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

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CD19, Polyclonal Antibody (Cat# AAA29305)

Full Name

CD19 Polyclonal Antibody

Gene Names

CD19; B4; CVID3

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Confocal immunofluorescence analysis of U2OS cells using KU70 Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using KU70 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using KU70 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using KU70 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using KU70 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using KU70 at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using KU70 at 1:1000 dilution.)

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KU70, Polyclonal Antibody (Cat# AAA30585)

Full Name

KU70 Rabbit Polyclonal Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Western blot analysis of HDAC5 expression in mouse brain tissue lysates, The lane on the right is treated with the antigen-specific peptide.)

IHC (Immunohistchemistry) (AAA31097 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31097 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31097 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31097 at 1/100 staining human breast carcinoma tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 ho)

IF (Immunofluorescence) (AAA31097 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of HDAC5 expression in HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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HDAC5, Polyclonal Antibody (Cat# AAA31097)

Full Name

HDAC5 Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HL-60 cells using anti-NLRX1 antibody (AAA19829).Overlay histogram showing HL-60 cells stained with AAA19829 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRX1 Antibody (AAA19829, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of NLRX1 using anti-NLRX1 antibody (AAA19829).NLRX1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-NLRX1 Antibody (AAA19829) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 5. IF analysis of NLRX1 using anti-NLRX1 antibody (AAA19829).NLRX1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NLRX1 Antibody (AAA19829) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NLRX1 Antibody (AAA19829) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NLRX1 Antibody (AAA19829) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: rat heart tissue lysates,Lane 6: rat skeletal muscle tissue lysates,Lane 7: mouse heart tissue lysates,Lane 8: mouse skeletal muscle tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRX1 antigen affinity purified polyclonal antibody (#AAA19829) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NLRX1 at approximately 108 kDa. The expected band size for NLRX1 is at 108 kDa.)

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NLRX1, Polyclonal Antibody (Cat# AAA19829)

Full Name

Anti-NLRX1 Antibody Picoband

Gene Names

NLRX1; NOD5; NOD9; NOD26; DLNB26; CLR11.3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

PPP1R15B, Polyclonal Antibody (Cat# AAA29301)

Full Name

PPP1R15B Polyclonal Antibody

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

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ILK, Polyclonal Antibody (Cat# AAA29048)

Full Name

ILK Polyclonal Antibody

Gene Names

ILK; P59; ILK-1; ILK-2; p59ILK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H2B-K12 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody.)

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Histone H2B-K12, Polyclonal Antibody (Cat# AAA29816)

Full Name

Acetyl-Histone H2B-K12 pAb

Gene Names

HIST2H2BE; H2B; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (WB Suggested Anti-OTX2 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: OTX2Sample Type: MCF7Antibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: OTX2Sample Type: JurkatAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: OTX2Sample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: OTX2Sample Type: HepG2Antibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: OTX2Sample Type: HelaAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Human Stomach)

IHC (Immunohistochemistry) (Human Heart)

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OTX2, Polyclonal Antibody (Cat# AAA23410)

Full Name

OTX2 antibody - N-terminal region

Gene Names

OTX2; CPHD6; MCOPS5

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug MAPK14 antibody. Western blot was performed from the immunoprecipitate using MAPK14 antibody at a dilition of 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using MAPK14 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using MAPK14 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using MAPK14 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using MAPK14 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MAPK14 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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MAPK14, Polyclonal Antibody (Cat# AAA28274)

Full Name

MAPK14 Polyclonal Antibody

Gene Names

MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

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11beta-HSD1, Polyclonal Antibody (Cat# AAA29298)

Full Name

11beta-HSD1 Polyclonal Antibody

Gene Names

HSD11B1; HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of PC-3 cells using anti-MTCH2 antibody (AAA19839).Overlay histogram showing PC-3 cells stained with AAA19839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTCH2 Antibody (AAA19839, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IP (Immunoprecipitation) (Figure 6. Immunoprecipitating MTCH2 in Hela whole cell lysate .Western blot analysis of MTCH2 using anti-MTCH2 antibody (AAA19839).Lane 1: Hela whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-MTCH2 antibody in Hela whole cell lysate.Lane 3: anti-MTCH2 antibody (2ug) + Hela whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MTCH2 antigen affinity purified polyclonal antibody (AAA19839) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MTCH2 Antibody (AAA19839) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MTCH2 Antibody (AAA19839) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MTCH2 Antibody (AAA19839) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCH2 antigen affinity purified polyclonal antibody (#AAA19839) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTCH2 at approximately 33 kDa. The expected band size for MTCH2 is at 33 kDa.)

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MTCH2, Polyclonal Antibody (Cat# AAA19839)

Full Name

Anti-MTCH2 Antibody Picoband

Gene Names

MTCH2; MIMP; HSPC032; SLC25A50

Reactivity

Human, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry-Paraffin) (Immunochemical staining of rat 2 in rat skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of rat 2 in rat liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of Mouse CPT2 in Mouse skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of Mouse CPT2 in Mouse liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human CPT2 in human muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human CPT2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

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CPT2, Polyclonal Antibody (Cat# AAA27775)

Full Name

Anti-CPT2 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence-Akt2 Polyclonal Antibody)

IF (Immunofluorescence) (Immunofluorescence-Akt2 Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-Akt2 Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-Akt2 Polyclonal Antibody)

IHC (Immunohistchemistry) (Immunohistochemistry-Akt2 Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-Akt2 Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-Akt2 Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-Akt2 Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-Akt2 Polyclonal Antibody)

WB (Western Blot) (Western blot-Akt2 Polyclonal Antibody)

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Akt2, Polyclonal Antibody (Cat# AAA28287)

Full Name

Akt2 Polyclonal Antibody

Gene Names

AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

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JIP-3, Polyclonal Antibody (Cat# AAA29055)

Full Name

JIP-3 Polyclonal Antibody

Gene Names

MAPK8IP3; syd; JIP3; SYD2; JSAP1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

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BMP-15, Polyclonal Antibody (Cat# AAA29311)

Full Name

BMP-15 Polyclonal Antibody

Gene Names

BMP15; ODG2; POF4; GDF9B

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110β (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110β (~110kD).)

IP (Immunoprecipitation) (Immunoprecipitation: 10ug of AAA14720 was used to immunoprecipitate from 500ug of Jurkat whole cell lysate. The antibody was collected on Protein A beads and eluted with sample buffer. 5uL of Jurkat whole cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 0.5ug of AAA14720. Proteins were visualized using anti-rabbit-HRP conjugate and an ECL system.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining of human skeletal muscle. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. AAA14720 diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern of cross banding striated muscle fibers.)

IHC (Immunohistochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining in colorectal carcinoma. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. AAA14720 diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a plasma membrane staining pattern.)

ICC (Immunocytochemistry) (Confocal Immunocytochemistry Analysis: HeLa cells were fixed, permeablized, and stained with AAA14720 (Cy3, red), DAPI (blue, nuclei), and Phalloidin-AlexaFluor®488 (actin, green). Figure on the left has the DAPI filter off.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining of human kidney 2 mm array spot. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. AAA14720 diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is see as a staining to include the thin and distal microtubules.)

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Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA14720)

Full Name

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Gene Names

PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237

Reactivity

Human

Applications

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purity

Affinity Purified
Purified by immunoaffinity chromatography.

Pricing

$885/0.1 mL $1,715/2x0.1 mL $2,495/3x0.1 mL $3,235/4x0.1 mL $3,755/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HepG2 cells using anti-Vaspin/SERPINA12 antibody (AAA19840).Overlay histogram showing HepG2 cells stained with AAA19840 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Vaspin/SERPINA12 using anti-Vaspin/SERPINA12 antibody (AAA19840).Vaspin/SERPINA12 was detected in a paraffin-embedded section of adipose of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse B16-F10 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vaspin/SERPINA12 antigen affinity purified polyclonal antibody (#AAA19840) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Vaspin/SERPINA12 at approximately 55 kDa. The expected band size for Vaspin/SERPINA12 is at 47 kDa.)

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Vaspin/SERPINA12, Polyclonal Antibody (Cat# AAA19840)

Full Name

Anti-Vaspin/SERPINA12 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (CAV1/Caveolin 1 Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (CAV1/Caveolin 1 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CAV1/Caveolin 1 Antibody-Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (CAV1/Caveolin 1 Antibody-Western Blot; Sample: Recombinant protein.)

WB (Western Blot) (CAV1/Caveolin 1 Antibody-Western blot of recombinant CAV1/Caveolin 1. This image was taken for the unconjugated form of this product. Other forms have not been tested.)

WB (Western Blot) (CAV1/Caveolin 1 Antibody-Western Blot; Sample: Mouse Heart lysate; ;Primary Ab: 3ug/ml Rabbit Anti-Rat CAV1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

WB (Western Blot) (CAV1/Caveolin 1 Antibody-Western Blot; Sample: Rat Heart lysate; ;Primary Ab: 3ug/ml Rabbit Anti-Rat CAV1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

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CAV1/Caveolin 1, Polyclonal Antibody (Cat# AAA21374)

Full Name

CAV1/Caveolin 1 Rabbit anti-Rat Polyclonal (aa2-105) Antibody

Gene Names

CAV1; CGL3; BSCL3; VIP21; MSTP085

Reactivity

Mouse, Rat

Applications

Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ERK1/ ERK2 Polyclonal Antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ERK1/ ERK2 Polyclonal Antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ERK1/ ERK2 Polyclonal Antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using ERK1/ ERK2 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using ERK1/ ERK2 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ERK1/ ERK2 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ERK1/ ERK2 antibody.)

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ERK1/ERK2, Polyclonal Antibody (Cat# AAA29822)

Full Name

ERK1/ERK2 Polyclonal Antibody

Gene Names

MAPK1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using DES at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using DES at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using DES at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using DES at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using DES at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using DES.)

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DES, Polyclonal Antibody (Cat# AAA30590)

Full Name

DES Rabbit Polyclonal Antibody

Gene Names

DES; CSM1; CSM2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of COMT using anti-COMT antibody (AAA11647).COMT was detected in frozen section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COMT Antibody (AAA11647) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of COMT using anti-COMT antibody (AAA11647).COMT was detected in frozen section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COMT Antibody (AAA11647) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of COMT using anti-COMT antibody (AAA11647).COMT was detected in frozen section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COMT Antibody (AAA11647) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ICC (Immunocytochemistry) (Figure 5. IHC analysis of COMT using anti-COMT antibody (AAA11647).COMT was detected in immunocytochemical section of A549 Cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COMT Antibody (AAA11647) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of COMT using anti-COMT antibody (AAA11647).COMT was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COMT Antibody (AAA11647) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of COMT using anti-COMT antibody (AAA11647).COMT was detected in paraffin-embedded section of Rat Lung Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COMT Antibody (AAA11647) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of COMT using anti-COMT antibody (AAA11647).COMT was detected in paraffin-embedded section of Mouse Lung Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-COMT Antibody (AAA11647) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of COMT using anti-COMT antibody (AAA11647).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Brain Tissue LysateLane 2: Rat Liver Tissue LysateLane 3: Rat Kidney Tissue LysateLane 4: Mouse Brain Tissue LysateLane 5: JURKAT Whole Cell LysateLane 6: CEM Whole Cell LysateLane 7: HELA Whole Cell LysateAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMT antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COMT at approximately 30KD. The expected band size for COMT is at 30KD.)

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COMT, Polyclonal Antibody (Cat# AAA11647)

Full Name

Anti-COMT Antibody

Gene Names

COMT; HEL-S-98n

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)

Purity

Immunogen Affinity Purified

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

WB (Western Blot) (Western blotAll lanes: Bordella pertussis pertussis toxin subunit 1 antibody at 2ug/ml+recombinant Bordella pertussis pertussis toxin subunit 1 100ngSecondaryGoat polyclonal Rabbit IgG 1/1000 dilutionPredicted band size: 36kDaObserved band size: 36kDa)

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Bordetella pertussis Pertussis toxin subunit 1, Polyclonal Antibody (Cat# AAA18815)

Full Name

Rabbit anti-Bordetella pertussis Pertussis toxin subunit 1 polyclonal Antibody

Reactivity

Bordetella pertussis

Applications

ELISA (EIA), Western Blot (WB)

Purity

>95%, Protein G Purified

Pricing

$240/0.05 mg $340/0.1 mg $650/2x0.1 mg $945/3x0.1 mg $1,220/4x0.1 mg $1,445/5x0.1 mg

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WB (Western Blot) (WB Suggested Anti-TGFB1I1 Antibody Titration: 0.2-1 ug/mlPositive Control: Human Lung)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: TGFB1I1Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Human Lung)

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TGFB1I1, Polyclonal Antibody (Cat# AAA23425)

Full Name

TGFB1I1 Antibody - middle region

Gene Names

TGFB1I1; HIC5; ARA55; HIC-5; TSC-5

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

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Ki-67, Polyclonal Antibody (Cat# AAA29057)

Full Name

Ki-67 Polyclonal Antibody

Gene Names

MKI67; KIA

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (GFAP Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (GFAP Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (GFAP Antibody-Western Blot; Sample: Mouse Cerebrum lysate; ;Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

WB (Western Blot) (GFAP Antibody-Western blot of recombinant GFAP.)

WB (Western Blot) (GFAP Antibody-Western Blot; Sample: Rat Cerebrum lysate; ;Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

WB (Western Blot) (GFAP Antibody-Western Blot; Sample: Mouse Heart lysate; ;Primary Ab: 2ug/ml Rabbit Anti-Mouse GFAP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

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GFAP, Polyclonal Antibody (Cat# AAA21373)

Full Name

GFAP Rabbit anti-Mouse Polyclonal (aa253-354) Antibody

Reactivity

Mouse, Rat

Applications

Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Acetyl-H2A.Z ( K4/7/11/13) in lysates of HeLa cells(TSA 1M, 18 hr), using Acetyl-H2A.Z ( K4/7/11/13) Antibody(AF4366).)

WB (Western Blot) (Western blot analysis of extracts from HeLa cells(TSA 1M, 18 hr), using Acetyl-H2A.Z (Lys4/7/11/13) Antibody.Lane1 was treated with Ac-blocking peptide.Lane2 was treated with Non-Ac-blocking peptide.)

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H2A.Z, Polyclonal Antibody (Cat# AAA31357)

Full Name

Acetyl-H2A.Z (Lys4/7/11/13) Antibody

Gene Names

H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (91%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistchemistry) (ACE2/ACE-2 Antibody-Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

IHC (Immunohistochemistry) (ACE2/ACE-2 Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (ACE2/ACE-2 Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (ACE2/ACE-2 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (ACE2/ACE-2 Antibody-The anti-ACE2 C-term antibody is used in Western blot to detect ACE2 in 293 cell lysate.)

WB (Western Blot) (ACE2/ACE-2 Antibody-Western blot of anti-ACE2 C-term antibody in K562 cell line lysates (35 ug/lane). ACE2(arrow) was detected using the purified antibody.)

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ACE2/ACE-2, Polyclonal Antibody (Cat# AAA21371)

Full Name

ACE2/ACE-2 Rabbit anti-Human Polyclonal (aa773-805) Antibody

Gene Names

ACE2; ACEH

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Ammonium sulfate precipitation

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.