Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Phospho-MCM2 (Ser108) Antibody.Lane 1: MCF7 cells(UV treatment), blocked with antigen-specific peptides,Lane 2: MCF7 cells(UV treatment),Lane 3: Hela cells(LPS treatment),Lane 4: Hela cells(heat shock treatment).)

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MCM2, Polyclonal Antibody (Cat# AAA31328)

Full Name

Phospho-MCM2 (Ser108) Antibody

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Hela cells using anti-PACSIN2 antibody (AAA19809).Overlay histogram showing Hela cells stained with AAA19809 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PACSIN2 Antibody (AAA19809, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PACSIN2 using anti-PACSIN2 antibody (AAA19809).PACSIN2 was detected in a paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 7. IF analysis of PACSIN2 using anti-PACSIN2 antibody (AAA19809).PACSIN2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 6. IF analysis of PACSIN2 using anti-PACSIN2 antibody (AAA19809).PACSIN2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: monkey COS-7 whole cell lysates,Lane 3: human placenta tissue lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: rat lung tissue lysates,Lane 6: rat heart tissue tissue lysates,Lane 7: mouse lung tissue lysates,Lane 8: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACSIN2 antigen affinity purified polyclonal antibody (#AAA19809) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (60-65 kDa.)

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PACSIN2, Polyclonal Antibody (Cat# AAA19809)

Full Name

Anti-PACSIN2 Antibody Picoband

Gene Names

PACSIN2; SDPII

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: ANP32A konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa)

IP (Immunoprecipitation) (ANP32A was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ANP32A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ANP32A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa)

IF (Immunofluorescence) (Immunofluorescence staining of ANP32A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human ANP32A polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining of human ANP32A in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining of human ANP32A in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

WB (Western Blot) (Anti-ANP32A rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:28 kDa)

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ANP32A, Polyclonal Antibody (Cat# AAA27748)

Full Name

Anti-ANP32A Antibody, Rabbit Polyclonal

Gene Names

ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

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GATA-2/3, Polyclonal Antibody (Cat# AAA29028)

Full Name

GATA-2/3 Polyclonal Antibody

Gene Names

GATA3; HDR; HDRS

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CASP3 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using CASP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CASP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using CASP3 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using CASP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using CASP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using CASP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using CASP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using CASP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using CASP3 antibody.)

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Caspase3, Polyclonal Antibody (Cat# AAA29796)

Full Name

Caspase3 Polyclonal Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

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FAS-L, Polyclonal Antibody (Cat# AAA29023)

Full Name

FAS-L Polyclonal Antibody

Gene Names

FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-Tubulin Alpha antibody (AAA19805).Overlay histogram showing 293T cells stained with AAA19805 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tubulin Alpha Antibody (AAA19805, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of Tubulin Alpha using anti-Tubulin Alpha antibody (AAA19805).Tubulin Alpha was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Tubulin Alpha Antibody (AAA19805) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Tubulin Alpha Antibody (AAA19805) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Tubulin Alpha Antibody (AAA19805) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: rat liver tissue lysates,Lane 5: rat heart tissue lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse heart tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin Alpha antigen affinity purified polyclonal antibody (#AAA19805) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Tubulin Alpha at approximately 55 kDa. The expected band size for Tubulin Alpha is at 50 kDa.)

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Alpha Tubulin/TUBA1C, Polyclonal Antibody (Cat# AAA19805)

Full Name

Anti-Alpha Tubulin/TUBA1C Antibody Picoband

Gene Names

TUBA1C; TUBA6; bcm948

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TXN2 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using TXN2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using TXN2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using TXN2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using TXN2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TXN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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TXN2, Polyclonal Antibody (Cat# AAA28253)

Full Name

TXN2 Polyclonal Antibody

Gene Names

TXN2; TXN; MTRX; TRX2; MT-TRX; COXPD29

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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HMG-1, Polyclonal Antibody (Cat# AAA29277)

Full Name

HMG-1 Polyclonal Antibody

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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Hsp90 alpha, Polyclonal Antibody (Cat# AAA31325)

Full Name

Phospho-Hsp90 alpha (Thr5/Thr7) Antibody

Gene Names

HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CHRNA1 antibody. Blue: DAPI for nuclear staining.)

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CHRNA1, Polyclonal Antibody (Cat# AAA28261)

Full Name

CHRNA1 Polyclonal Antibody

Gene Names

CHRNA1; ACHRA; ACHRD; CHRNA; CMS1A; CMS1B; CMS2A; FCCMS; SCCMS

Reactivity

Human

Applications

Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse thymus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human normal tissues adjacent to liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Colo205, using Phospho-p38 MAPK (Thr180/Tyr182) Antibody. The lane on the left was treated with blocking peptide.)

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p38 MAPK, Polyclonal Antibody (Cat# AAA31333)

Full Name

Phospho-p38 MAPK (Thr180/Tyr182) Antibody

Gene Names

MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-HCLS1 Antibody Titration: 1.25ug/mlPositive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (WB Suggested Anti-HCLS1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (Host: MouseTarget Name: HCLS1Sample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-HCLS1 AntibodyParaffin Embedded Tissue: Human StomachCellular Data: Epithelial cells of Fundic GlandAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-HCLS1 AntibodyParaffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-HCLS1 AntibodyParaffin Embedded Tissue: Human HeartCellular Data: Myocardial cellsAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

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HCLS1, Polyclonal Antibody (Cat# AAA23398)

Full Name

HCLS1 antibody - N-terminal region

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of Vimentin Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Vimentin Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

FCM (Flow Cytometry) (Vimentin Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (Vimentin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (VIME Antibody western blot analysis in Hela,U251,A549,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the VIME antibody detected the VIME protein (arrow).)

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Vimentin, Polyclonal Antibody (Cat# AAA28770)

Full Name

Vimentin Antibody

Gene Names

VIM; HEL113; CTRCT30

Reactivity

Human

Applications

Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using Cytochrome c antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using Cytochrome c antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using Cytochrome c antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Cytochrome c antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Cytochrome c antibody at 1:1000 dilution.)

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Cytochrome c, Polyclonal Antibody (Cat# AAA30562)

Full Name

Cytochrome c Polyclonal Antibody

Gene Names

CYCS; CYC; HCS; THC4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (KRT5/CK5/Cytokeratin 5 Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (KRT5/CK5/Cytokeratin 5 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (KRT5/CK5/Cytokeratin 5 Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (KRT5/CK5/Cytokeratin 5 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (KRT5/CK5/Cytokeratin 5 Antibody-Western blot analysis of Cytokeratin 5 expression in DU145 (A); HeLa (B); MCF7 (C) whole cell lysates.)

ICC (Immunocytochemistry) (KRT5/CK5/Cytokeratin 5 Antibody-Immunofluorescent analysis of Cytokeratin 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

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KRT5/CK5/Cytokeratin 5, Polyclonal Antibody (Cat# AAA21347)

Full Name

KRT5/CK5/Cytokeratin 5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Gene Names

KRT5; K5; CK5; DDD; EBS2; KRT5A

Reactivity

Mouse, Bovine, Rat, Pig, Human

Applications

Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Immunoaffinity purified

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WB (Western Blot) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistchemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

WB (Western Blot) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

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Glut4, Polyclonal Antibody (Cat# AAA29286)

Full Name

Glut4 Polyclonal Antibody

Gene Names

SLC2A4; GLUT4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of PC12 cells using EGFR antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using EGFR antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody.)

WB (Western Blot) (Western blot analysis of extracts of OVCAR3 cells, using EGFR antibody.)

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EGFR, Polyclonal Antibody (Cat# AAA29798)

Full Name

EGFR Polyclonal Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of PC-3 cells using anti-RBM15 antibody (AAA19815).Overlay histogram showing PC-3 cells stained with AAA19815 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15 Antibody (AAA19815, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of RBM15 using anti-RBM15 antibody (AAA19815).RBM15 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM15 antigen affinity purified polyclonal antibody (#AAA19815) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBM15 at approximately 107 kDa. The expected band size for RBM15 is at 107 kDa.)

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RBM15, Polyclonal Antibody (Cat# AAA19815)

Full Name

Anti-RBM15 Antibody Picoband

Gene Names

RBM15; OTT; OTT1; SPEN

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-HCLS1 Antibody Titration: 1.25ug/mlPositive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (WB Suggested Anti-HCLS1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: JurkatHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (Host: MouseTarget Name: HCLS1Sample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-HCLS1 AntibodyParaffin Embedded Tissue: Human StomachCellular Data: Epithelial cells of Fundic GlandAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-HCLS1 AntibodyParaffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-HCLS1 AntibodyParaffin Embedded Tissue: Human HeartCellular Data: Myocardial cellsAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

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HCLS1, Polyclonal Antibody (Cat# AAA23399)

Full Name

HCLS1 antibody - N-terminal region

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Protein A purified

Pricing

$445/0.1 mL $855/2x0.1 mL $1,245/3x0.1 mL $1,610/4x0.1 mL $1,800/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using DDX5 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using DDX5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using DDX5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using DDX5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human oophoroma using DDX5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using DDX5 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using DDX5 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DDX5 antibody at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

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DDX5, Polyclonal Antibody (Cat# AAA28263)

Full Name

DDX5 Polyclonal Antibody

Gene Names

DDX5; p68; HLR1; G17P1; HUMP68

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistchemistry) (ATG16L1/ATG16L Antibody-Human Colon, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (ATG16L1/ATG16L Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (ATG16L1/ATG16L Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (ATG16L1/ATG16L Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (ATG16L1/ATG16L Antibody-Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (ATG16L1/ATG16L Antibody-Anti-ATG16L1 antibody (AAA21359, 20 ug/mL) yields a specific band on capillary Western analysis (Protein Simple, WES 12-230 kDa separation module) in 1 ng purified recombinant human ATG16L1 protein.)

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ATG16L1/ATG16L, Polyclonal Antibody (Cat# AAA21359)

Full Name

ATG16L1/ATG16L Rabbit anti-Human Polyclonal Antibody

Gene Names

ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P), Simple Western Blot (SWB)

Purity

Immunoaffinity purified

Pricing

$650/0.05 mg $1,255/2x0.05 mg $1,825/3x0.05 mg $2,365/4x0.05 mg $2,680/5x0.05 mg

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WB (Western Blot) (FOLR2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FOLR2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of lysate from mouse heart tissue lysate, using FOLR2 Antibody (N-term). AAA28783 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

WB (Western Blot) (Western blot analysis of lysates from KG-1, MCF-7, MDA-MB-453 cell line (from left to right), using FOLR2 Antibody (N-term). AAA28783 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from human placenta tissue lysate, using FOLR2 Antibody (N-term). AAA28783 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

WB (Western Blot) (Western blot analysis of lysate from human placenta tissue lysate, using FOLR2 Antibody (N-term). AAA28783 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)

WB (Western Blot) (Western blot analysis of lysates from MDA-MB-453 cell line, human placenta tissue lysate (from left to right), using FOLR2 Antibody (N-term). AAA28783 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from human placenta tissue, using FOLR2 Antibody (N-term). AAA28783 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

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FOLR2, Polyclonal Antibody (Cat# AAA28783)

Full Name

FOLR2 Antibody (N-term)

Gene Names

FOLR2; FBP; FR-P3; FR-BETA; BETA-HFR; FBP/PL-1

Reactivity

Human, mouse

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using Acetyl-Histone H4-K12 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H4-K12 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H4-K12 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using Acetyl-Histone H4-K12 antibody.)

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Histone H4-K12, Polyclonal Antibody (Cat# AAA29807)

Full Name

Acetyl-Histone H4-K12 pAb

Gene Names

HIST2H4B; H4/o

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Human Lung Cancer Tissue)

IHC (Immunohistchemistry) (Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

IHC (Immunohistochemistry) (Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Rat Skeletal Muscle Tissue)

IHC (Immunohistochemistry) (Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohistochemistry) (Anti- IRS1 antibody, AAA11632, IHC(P)IHC(P): Mouse Intestine Tissue)

WB (Western Blot) (Anti- IRS1 antibody, AAA11632, Western blottingAll lanes: Anti IRS1 (AAA11632) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: MM453 Whole Cell Lysate at 40ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 132KDObserved bind size: 132KD)

WB (Western Blot) (Anti- IRS1 antibody, AAA11632, Western blottingAll lanes: Anti IRS1 (AAA11632) at 0.5ug/mlWB: Recombinant Human IRS1 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)

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Insulin receptor substrate 1, Polyclonal Antibody (Cat# AAA11632)

Full Name

Anti-IRS1 Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat. No cross reactivity with other proteins.

Applications

Western Blot (WB), Immunohistochemistry (IHC-P)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using NFE2L1 antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using NFE2L1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using NFE2L1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterus using NFE2L1 antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using NFE2L1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using NFE2L1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using NFE2L1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using NFE2L1 antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using NFE2L1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NFE2L1 antibody at 1:1000 dilution.)

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NFE2L1, Polyclonal Antibody (Cat# AAA30570)

Full Name

NFE2L1 Polyclonal Antibody

Gene Names

NFE2L1; NRF1; TCF11; LCR-F1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (CTSD/Cathepsin D Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CTSD/Cathepsin D Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CTSD/Cathepsin D Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CTSD/Cathepsin D Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CTSD/Cathepsin D Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (CTSD/Cathepsin D Antibody-Western blot analysis of Cathepsin D LC expression in HepG2 colchicine-treated (A); mouse heart (B); rat heart (C) whole cell lysates.)

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CTSD/Cathepsin D, Polyclonal Antibody (Cat# AAA21355)

Full Name

CTSD/Cathepsin D Rabbit anti-Human Polyclonal (Internal) Antibody

Gene Names

CTSD; CPSD; CLN10

Reactivity

Mouse, Rat, Pig, Human

Applications

Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Immunoaffinity purified

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

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HDAC5/9, Polyclonal Antibody (Cat# AAA29035)

Full Name

HDAC5/9 Polyclonal Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

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AR, Polyclonal Antibody (Cat# AAA29291)

Full Name

AR Polyclonal Antibody

Gene Names

AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Host: RabbitTarget Name: GSCSample Type: 786-0 Whole Cell lysatesAntibody Dilution: 10ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GSCSample Type: 786-0 Whole Cell lysatesAntibody Dilution: 0.25ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GSCSample Type: 786-0 Whole Cell lysatesAntibody Dilution: 0.25ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GSCSample Tissue: Human DLD1Antibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GSCSample Tissue: Human 786-0 Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Immunohistochemistry with Human Tonsil lysate tissue at an antibody concentration of 5.0ug/ml using anti-GSC antibody)

IHC (Immunohistochemistry) (Immunohistochemistry with Human Testis lysate tissue at an antibody concentration of 5.0ug/ml using anti-GSC antibody)

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GSC, Polyclonal Antibody (Cat# AAA23404)

Full Name

GSC antibody - N-terminal region

Gene Names

GSC; SAMS

Reactivity

Cow, Dog, Human, Mouse, Pig, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

WB (Western Blot) (Western BlotPositive WB detected in Recombinant proteinAll lanes: dnaK antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 70 kDaobserved band size: 70 kDa)

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dnaK, Polyclonal Antibody (Cat# AAA26988)

Full Name

dnaK Antibody

Reactivity

Escherichia coli

Applications

ELISA (EIA), Western Blot (WB)

Purity

> 95%, Protein G purified

Pricing

$240/0.05 mg $340/0.1 mg $650/2x0.1 mg $945/3x0.1 mg $1,220/4x0.1 mg $1,445/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using CDA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using CDA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using CDA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using CDA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using CDA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using CDA Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CDA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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CDA, Polyclonal Antibody (Cat# AAA28268)

Full Name

CDA Polyclonal Antibody

Gene Names

CDA; CDD

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of NOS2A Antibody (Center) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

FCM (Flow Cytometry) (NOS2A Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (NOS2A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NOS2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (NOS2A Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOS2A antibody detected the NOS2A protein (arrow).)

WB (Western Blot) (Anti-NOS2A Antibody (Center)at 1:2000 dilution + A549 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 131 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-NOS2A Antibody (Center)at 1:2000 dilution + Y79 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 131 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

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NOS2A, Polyclonal Antibody (Cat# AAA28780)

Full Name

NOS2A Antibody (Center)

Gene Names

NOS2; NOS; INOS; NOS2A; HEP-NOS

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using BAX antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using BAX antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using BAX antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using BAX antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using BAX antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using BAX antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using BAX antibody.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and BAX knockout (KO) 293T cells, using BAX antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BAX antibody.)

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BAX, Polyclonal Antibody (Cat# AAA29801)

Full Name

BAX Polyclonal Antibody

Gene Names

BAX; BCL2L4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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Application Data (At 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry) (At 1/100 staining human lung carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extract from HT29 cells using N-Cadherin antibody.)

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N Cadherin, Polyclonal Antibody (Cat# AAA31337)

Full Name

N Cadherin Antibody

Gene Names

CDH2; CDHN; NCAD; CD325; CDw325

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA), Immunohistochemistry Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using AK1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using AK1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using AK1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using AK1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using AK1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using AK1 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using AK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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AK1, Polyclonal Antibody (Cat# AAA28266)

Full Name

AK1 Polyclonal Antibody

Gene Names

AK1; HTL-S-58j

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

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GTBP, Polyclonal Antibody (Cat# AAA29034)

Full Name

GTBP Polyclonal Antibody

Gene Names

MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5

Reactivity

Human, Mouse, Monkey

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

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Ub, Polyclonal Antibody (Cat# AAA29292)

Full Name

Ub Polyclonal Antibody

Gene Names

UBA52; L40; CEP52; RPL40; HUBCEP52

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HAL Polyclonal Antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using HAL antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using HAL antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using HAL antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using HAL antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HAL antibody.)

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HAL, Polyclonal Antibody (Cat# AAA29804)

Full Name

HAL Polyclonal Antibody

Gene Names

HAL; HIS; HSTD

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (WB Suggested Anti-HDAC1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:2500Positive Control: Jurkat cell lysateHDAC1 is supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (Host: RabbitTarget Name: HDAC1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: HDAC1Sample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: HDAC1Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: HDAC1Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-HDAC1 antibodyParaffin Embedded Tissue: Human Heart cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

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HDAC1, Polyclonal Antibody (Cat# AAA23405)

Full Name

HDAC1 antibody - middle region

Gene Names

HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

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C4a/b, Polyclonal Antibody (Cat# AAA29293)

Full Name

C4a/b Polyclonal Antibody

Gene Names

C4A; C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using DAPK3 antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human placenta using DAPK3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using DAPK3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using DAPK3 antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using DAPK3 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using DAPK3 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DAPK3 antibody at 1:1000 dilution.)

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DAPK3, Polyclonal Antibody (Cat# AAA30573)

Full Name

DAPK3 Polyclonal Antibody

Gene Names

DAPK3; ZIP; ZIPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

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CD63, Polyclonal Antibody (Cat# AAA29287)

Full Name

CD63 Polyclonal Antibody

Gene Names

CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) ((0.5ug/ml) staining of A549 (A), HepG2 (B) and K562 (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) ((0.3ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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TNFRSF1A/TNFR1, Polyclonal Antibody (Cat# AAA13672)

Full Name

Goat anti-TNFRSF1A/TNFR1 Antibody

Gene Names

TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2

Reactivity

Tested: Human; Expected from sequence similarity: Human, Rat, Pig

Applications

Peptide ELISA (EIA), Western Blot (WB)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$455/0.1 mg $875/2x0.1 mg $1,270/3x0.1 mg $1,645/4x0.1 mg $1,815/5x0.1 mg

WB (Western Blot) (Western BlotPositive WB detected in Recombinant proteinAll lanes: mpt64 antibody at 1.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 25 KDaObserved band size: 25 KDa)

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mpt64, Polyclonal Antibody (Cat# AAA26984)

Full Name

mpt64 Antibody

Reactivity

Mycobacterium tuberculosis

Applications

ELISA (EIA), Western Blot (WB)

Purity

>95%, Protein G purified

Pricing

$240/0.05 mg $340/0.1 mg $650/2x0.1 mg $945/3x0.1 mg $1,220/4x0.1 mg $1,445/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using PD-1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using PD-1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse bronchial epithelium using PD-1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human skin using PD-1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat bronchial epithelium using PD-1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PD-1 antibody.)

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PD-1, Polyclonal Antibody (Cat# AAA29800)

Full Name

PD-1 Polyclonal Antibody

Gene Names

PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse liver using HMGCS2 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of human liver using HMGCS2 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat liver using HMGCS2 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using HMGCS2 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using HMGCS2 antibody at dilution of 1:100 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HMGCS2 antibody at 1:1000 dilution.)

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HMGCS2, Polyclonal Antibody (Cat# AAA30568)

Full Name

HMGCS2 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (Anti- gamma Catenin Picoband antibody, AAA11635, IHC(F)IHC(F): Rat Intestine Tissue)

IHC (Immunohistochemistry) (Anti- gamma Catenin Picoband antibody, AAA11635, IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohistochemistry) (Anti- gamma Catenin Picoband antibody, AAA11635, IHC(P)IHC(P): Mouse Intestine Tissue)

IHC (Immunohistochemistry) (Anti- gamma Catenin Picoband antibody, AAA11635, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

WB (Western Blot) (Anti- gamma Catenin Picoband antibody, AAA11635, Western blottingAll lanes: Anti gamma Catenin (AAA11635) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Cardiac Muscle Tissue Lysate at 50ugLane 3: Rat Thymus Tissue Lysate at 50ugLane 4: RH35 Whole Cell Lysate at 40ugLane 5: Hela Whole Cell Lysate at 40ugLane 6: Colo320 Whole Cell Lysate at 40ugLane 7: HepG2 Whole Cell Lysate at 40ugLane 8: HepA Whole Cell Lysate at 40ugLane 9: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 81KD Observed bind size: 81KD)

WB (Western Blot) (Anti- gamma Catenin Picoband antibody, AAA11635, Western blottingAll lanes: Anti gamma Catenin(AAA11635) at 0.5ug/mlWB: Recombinant Human gamma Catenin Protein 0.5ngPredicted bind size: 47KDObserved bind size: 47KD)

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Junction plakoglobin, Polyclonal Antibody (Cat# AAA11635)

Full Name

Anti-gamma Catenin Antibody

Gene Names

JUP; DP3; PDGB; PKGB; CTNNG; DPIII; ARVD12

Reactivity

Human, Mouse, Rat. No cross reactivity with other proteins.

Applications

Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-F)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of JK cells using anti-UT-B/SLC14A1 antibody (AAA19827).Overlay histogram showing JK cells stained with AAA19827 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-UT-B/SLC14A1 Antibody (AAA19827, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of UT-B/SLC14A1 using anti-UT-B/SLC14A1 antibody (AAA19827).UT-B/SLC14A1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-UT-B/SLC14A1 Antibody (AAA19827) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-UT-B/SLC14A1 Antibody (AAA19827) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-UT-B/SLC14A1 Antibody (AAA19827) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-UT-B/SLC14A1 Antibody (AAA19827) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-UT-B/SLC14A1 Antibody (AAA19827) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-UT-B/SLC14A1 Antibody (AAA19827) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-UT-B/SLC14A1 Antibody (AAA19827) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-UT-B/SLC14A1 Antibody (AAA19827) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human PC-3 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: rat heart tissue lysates,Lane 6: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UT-B/SLC14A1 antigen affinity purified polyclonal antibody (#AAA19827) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UT-B/SLC14A1 at approximately 65 kDa. The expected band size for UT-B/SLC14A1 is at 43 kDa.)

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UT-B/SLC14A1, Polyclonal Antibody (Cat# AAA19827)

Full Name

Anti-UT-B/SLC14A1 Antibody Picoband

Gene Names

SLC14A1; JK; UT1; UTE; HUT11; RACH1; RACH2; UT-B1; HsT1341

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (CX3CL1/Fractalkine Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CX3CL1/Fractalkine Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CX3CL1/Fractalkine Antibody-Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CX3CL1/Fractalkine Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CX3CL1/Fractalkine Antibody-Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CX3CL1/Fractalkine Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

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CX3CL1/Fractalkine, Polyclonal Antibody (Cat# AAA21363)

Full Name

CX3CL1/Fractalkine Rabbit anti-Human Polyclonal Antibody

Gene Names

CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Purity

Immunoaffinity purified

Pricing

$650/0.05 mg $1,255/2x0.05 mg $1,825/3x0.05 mg $2,365/4x0.05 mg $2,680/5x0.05 mg

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.