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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2900-2950 of 96812 product results
WB (Western Blot)
(Figure 1. Western blot analysis of Cytochrome C using anti-Cytochrome C antibody (AAA124711).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysate,Lane 2: rat heart tissue lysate,Lane 3: mouse brain tissue lysate,Lane 4: mouse heart tissue lysate,Lane 5: human Hela whole Cell lysate,Lane 6: human MCF-7 whole Cell lysate,Lane 7: human 293T whole Cell lysate,Lane 8: human HepG2 whole Cell lysate,Lane 9: human Jurkat whole Cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome C antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cytochrome C at approximately 14KD. The expected band size for Cytochrome C is at 12KD.)
Cytochrome C, Polyclonal Antibody (Cat# AAA124711)
Full Name
Anti-Cytochrome C Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of PLCG 2 using anti-PLCG 2 antibody (AAA124712).PLCG 2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLCG 2 Antibody (AAA124712) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
PLCG 2, Polyclonal Antibody (Cat# AAA124712)
Full Name
Anti-PLCG 2 Picoband Antibody
Gene Names
PLCG2; FCAS3; APLAID; PLC-IV; PLC-gamma-2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of MMP10 using anti-MMP10 antibody.MMP10 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MMP10 Antibody (A03759) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
MMP10, Polyclonal Antibody (Cat# AAA124714)
Full Name
Anti-MMP10 Picoband Antibody
Gene Names
MMP10; SL-2; STMY2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (AAA124715).Kallikrein 2 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Kallikrein 2 Antibody (AAA124715) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Kallikrein 2, Polyclonal Antibody (Cat# AAA124715)
Full Name
Anti-Kallikrein 2 Picoband antibody
Gene Names
KLK2; hK2; hGK-1; KLK2A2
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U20S cells using anti-Perilipin A antibody (AAA124716).Overlay histogram showing U20S cells stained with AAA124716 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Perilipin A Antibody (AAA124716,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Perilipin A, Polyclonal Antibody (Cat# AAA124716)
Full Name
Anti-Perilipin A Picoband Antibody
Gene Names
PLIN1; PERI; PLIN; FPLD4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of SAP102 using anti-SAP102 antibody (AAA124719).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP102 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SAP102 at approximately 102KD. The expected band size for SAP102 is at 90KD.)
SAP102, Polyclonal Antibody (Cat# AAA124719)
Full Name
Anti-SAP102 Picoband Antibody
Gene Names
DLG3; MRX; XLMR; MRX90; NEDLG; SAP102; PPP1R82
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of FZD3 using anti-FZD3 antibody (AAA124724).FZD3 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FZD3 Antibody (AAA124724) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FZD3/Frizzled 3, Polyclonal Antibody (Cat# AAA124724)
Full Name
Anti-FZD3/Frizzled 3 antibody
Gene Names
FZD3; Fz-3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Figure 2. Western blot analysis of GADD45G using anti-GADD45G antibody (AAA124725).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human SW620 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: mouse NIH3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.)
GADD45G, Polyclonal Antibody (Cat# AAA124725)
Full Name
Anti-GADD45G Antibody
Gene Names
GADD45G; CR6; DDIT2; GRP17; GADD45gamma
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of TMEM107 using anti- TMEM107 antibody (AAA124726).TMEM107 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- TMEM107 Antibody (AAA124726) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
TMEM107, Polyclonal Antibody (Cat# AAA124726)
Full Name
Anti-TMEM107 Picoband Antibody
Gene Names
TMEM107; MKS13; JBTS29; GRVS638; PRO1268
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of MUC7 using anti-MUC7 antibody (AAA124729).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human SW620 whole cell lysates,Lane 2: human HepG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC7 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MUC7 at approximately 46KD. The expected band size for MUC7 is at 39KD.)
MUC7, Polyclonal Antibody (Cat# AAA124729)
Full Name
Anti-MUC7 Picoband Antibody
Gene Names
MUC7; MG2
Applications
Western Blot
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (AAA124733).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOV/CCN3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOV/CCN3 at approximately 47KD. The expected band size for NOV/CCN3 is at 39KD.)
NOV/CCN3, Polyclonal Antibody (Cat# AAA124733)
Full Name
Anti-NOV/CCN3 Picoband Antibody
Gene Names
NOV; CCN3; NOVh; IBP-9; IGFBP9; IGFBP-9
Reactivity
Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of TFF2 using anti-TFF2 antibody (AAA124736).TFF2 was detected in paraffin-embedded section of rat gaster tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TFF2 Antibody (AAA124736) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
TFF2, Polyclonal Antibody (Cat# AAA124736)
Full Name
Anti-TFF2 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA119647 at dilution 1:100)
ATF7, Polyclonal Antibody (Cat# AAA119647)
Full Name
Rabbit anti-human ATF7 polyclonal Antibody
Gene Names
ATF7; ATFA
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(Immunofluorescent analysis of LO2 cells using AAA119649 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
CATSPER2, Polyclonal Antibody (Cat# AAA119649)
Full Name
CATSPER2 Antibody
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
>95%, Protein G Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using AAA119651 at dilution 1:100)
Cell adhesion molecule 1, Polyclonal Antibody (Cat# AAA119651)
Full Name
Rabbit anti-human Cell adhesion molecule 1 polyclonal Antibody(CADM1)
Gene Names
CADM1; BL2; ST17; IGSF4; NECL2; RA175; TSLC1; IGSF4A; Necl-2; SYNCAM; sgIGSF; sTSLC-1; synCAM1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human lung tissue using AAA119658 at dilution 1:100)
CCL11, Polyclonal Antibody (Cat# AAA119658)
Full Name
CCL11 Antibody
Gene Names
CCL11; SCYA11
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human pancreas using AAA119661 at dilution 1:100)
Transforming growth factor beta regulator 1, Polyclonal Antibody (Cat# AAA119661)
Full Name
Rabbit anti-human Transforming growth factor beta regulator 1 polyclonal Antibody(TBRG1)
Gene Names
TBRG1; NIAM; TB-5
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human endometrial cancer using AAA119663 at dilution 1:100)
Neuromedin-K receptor, Polyclonal Antibody (Cat# AAA119663)
Full Name
Rabbit anti-human Neuromedin-K receptor polyclonal Antibody(TACR3)
Gene Names
TACR3; NKR; HH11; NK3R; NK-3R; TAC3RL
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human testis using AAA119666 at dilution 1:100)
AIMP2, Polyclonal Antibody (Cat# AAA119666)
Full Name
AIMP2 Antibody
Gene Names
AIMP2; P38; JTV1; JTV-1; PRO0992
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA119667 at dilution 1:100)
Interleukin-1 receptor type 1, Polyclonal Antibody (Cat# AAA119667)
Full Name
Rabbit anti-human Interleukin-1 receptor type 1 polyclonal Antibody(IL1R1)
Gene Names
IL1R1; P80; IL1R; IL1RA; CD121A; D2S1473; IL-1R-alpha
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human kidney using AAA119670 at dilution 1:100)
Glycine amidinotransferase, Polyclonal Antibody (Cat# AAA119670)
Full Name
Rabbit anti-human Glycine amidinotransferase, mitochondrial polyclonal Antibody(GATM)
Gene Names
GATM; AT; AGAT; CCDS3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of LO2 cells using AAA119671 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
PQLC3, Polyclonal Antibody (Cat# AAA119671)
Full Name
PQLC3 Antibody
Gene Names
PQLC3; C2orf22
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
>95%, Protein G Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating PDLIM1 in HepG2 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA119672 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA119672(4ug)+ HepG2 whole cell lysate(500ug)Lane 3: HepG2 whole cell lysate (20ug))
PDZ and LIM domain protein 1, Polyclonal Antibody (Cat# AAA119672)
Full Name
Rabbit anti-human PDZ and LIM domain protein 1 polyclonal Antibody(PDLIM1)
Gene Names
PDLIM1; CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human placenta using AAA119677 at dilution 1:100)
NKG2-A/NKG2-B type II integral membrane protein, Polyclonal Antibody (Cat# AAA119677)
Full Name
Rabbit anti-human NKG2-A/NKG2-B type II integral membrane protein polyclonal Antibody(KLRC1)
Gene Names
KLRC1; NKG2; NKG2A; CD159A
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human testis using AAA119682 at dilution 1:100)
Mitochondrial brown fat uncoupling protein 1, Polyclonal Antibody (Cat# AAA119682)
Full Name
Rabbit anti-human Mitochondrial brown fat uncoupling protein 1 polyclonal Antibody(UCP1)
Gene Names
UCP1; UCP; SLC25A7
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating LCK in Jurkat whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA119688 in Jurkat whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA119688 (8ug)+ Jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (10ug))
Tyrosine-protein kinase Lck, Polyclonal Antibody (Cat# AAA119688)
Full Name
Rabbit anti-human Tyrosine-protein kinase Lck polyclonal Antibody
Gene Names
LCK; LSK; YT16; IMD22; p56lck; pp58lck
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human small intestine using AAA119689 at dilution 1:100)
Kynurenine-oxoglutarate transaminase 3, Polyclonal Antibody (Cat# AAA119689)
Full Name
Rabbit anti-human Kynurenine-oxoglutarate transaminase 3 polyclonal Antibody
Gene Names
CCBL2; KAT3; KATIII
Reactivity
Human
Applications
Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating P2RY14 in HepG2 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA119699 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA119699(4ug)+ HepG2 whole cell lysate(500ug)Lane 3: HepG2 whole cell lysate (20ug))
P2RY14, Polyclonal Antibody (Cat# AAA119699)
Full Name
Rabbit anti-human P2RY14 polyclonal Antibody
Gene Names
P2RY14; P2Y14; BPR105; GPR105
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating HARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA119700 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA119700(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
Histidine-tRNA ligase, Polyclonal Antibody (Cat# AAA119700)
Full Name
Rabbit anti-human Histidine-tRNA ligase, cytoplasmic polyclonal Antibody(HARS)
Gene Names
HARS; HRS; USH3B
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human placenta tissue using AAA119702 at dilution 1:100)
Sulfotransferase 1A3, Polyclonal Antibody (Cat# AAA119702)
Full Name
Rabbit anti-human Sulfotransferase 1A3 polyclonal Antibody(SULT1A3)
Gene Names
SULT1A4; HAST3; M-PST; ST1A4; TL-PST; ST1A3/ST1A4
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of MCF-7 cells using AAA119704 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Nuclear RNA export factor 3, Polyclonal Antibody (Cat# AAA119704)
Full Name
Rabbit anti-human Nuclear RNA export factor 3 polyclonal Antibody
Reactivity
Human
Applications
Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human pancreatic cancer using AAA119705 at dilution of 1:100)
YIPF6, Polyclonal Antibody (Cat# AAA119705)
Full Name
YIPF6 Antibody
Gene Names
YIPF6; FinGER6
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry
Purity
>95%, Protein G Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human skin using AAA119706 at dilution 1:100)
Patatin-like phospholipase domain-containing protein 3, Polyclonal Antibody (Cat# AAA119706)
Full Name
Rabbit anti-human Patatin-like phospholipase domain-containing protein 3 polyclonal Antibody(PNPLA3)
Gene Names
PNPLA3; ADPN; C22orf20; iPLA(2)epsilon
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using AAA119707 at dilution 1:100)
Splicing factor U2AF, Polyclonal Antibody (Cat# AAA119707)
Full Name
Rabbit anti-human Splicing factor U2AF 35 kDa subunit polyclonal Antibody(U2AF1)
Gene Names
U2AF1; RN; FP793; U2AF35; U2AFBP; RNU2AF1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(Immunofluorescent analysis of Hela cells cells using AAA119712 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Poly [ADP-ribose] polymerase 3, Polyclonal Antibody (Cat# AAA119712)
Full Name
Rabbit anti-human Poly [ADP-ribose] polymerase 3 polyclonal Antibody(PARP3)
Gene Names
PARP3; IRT1; ARTD3; ADPRT3; ADPRTL2; ADPRTL3; PADPRT-3
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using AAA119719 at dilution of 1:100)
DIP2A, Polyclonal Antibody (Cat# AAA119719)
Full Name
DIP2A Antibody
Gene Names
DIP2A; DIP2; C21orf106
Reactivity
Human
Applications
Immunohistochemistry
Purity
>95%, Protein G Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human colon cancer tissue using AAA119721 at dilution 1:100)
Cholecystokinin, Polyclonal Antibody (Cat# AAA119721)
Full Name
Rabbit anti-human Cholecystokinin polyclonal Antibody(CCK)
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human colon cancer using AAA119723 at dilution of 1:100)
TTC38, Polyclonal Antibody (Cat# AAA119723)
Full Name
TTC38 Antibody
Gene Names
TTC38; LL22NC03-5H6.5
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
>95%, Protein G Purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human pancreas tissue using AAA119725 at dilution 1:100)
C-Myc-binding, Polyclonal Antibody (Cat# AAA119725)
Full Name
Rabbit anti-human C-Myc-binding polyclonal Antibody(MYCBP)
Gene Names
MYCBP; AMY-1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human testis using AAA119729 at dilution 1:100)
Diacylglycerol kinase epsilon, Polyclonal Antibody (Cat# AAA119729)
Full Name
Rabbit anti-human Diacylglycerol kinase epsilon polyclonal Antibody(DGKE)
Gene Names
DGKE; DGK; DAGK5; DAGK6; NPHS7
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
FCM/FACS (Flow Cytometry)
(Figure 1. Flow Cytometry analysis of A431 cells using anti-Human SUR1 antibody.Overlay histogram showing A431 cells stained with A00895-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human SUR1 Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SUR1, Polyclonal Antibody (Cat# AAA124911)
Full Name
Anti-Human SUR1 DyLight 488 conjugated Antibody
Gene Names
ABCC8; HI; SUR; HHF1; MRP8; PHHI; SUR1; ABC36; HRINS; TNDM2; SUR1delta2
Reactivity
Human
Applications
Flow Cytometry
Purity
Immunogen Affinity Purified
Pricing
LATS1, Polyclonal Antibody (Cat# AAA124916)
Full Name
Anti-LATS1 Antibody
Gene Names
LATS1; wts; WARTS
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
Cannabinoid Receptor I, Polyclonal Antibody (Cat# AAA124922)
Full Name
Anti-Human Cannabinoid Receptor I DyLight 550 conjugated Antibody
Gene Names
CNR1; CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5
Reactivity
Human
Applications
Flow Cytometry
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of FLIP using anti-FLIP antibody.FLIP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-FLIP antibody.FLIP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-FLIP Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FLIP/CFLAR, Polyclonal Antibody (Cat# AAA124923)
Full Name
Anti-FLIP/CFLAR Antibody
Gene Names
CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of DDT using anti-DDT antibody.DDT was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT antibody.DDT was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT antibody.DDT was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT antibody.DDT was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT antibody.DDT was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT antibody.DDT was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT antibody.DDT was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT antibody.DDT was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT antibody.DDT was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DDT Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
DDT, Polyclonal Antibody (Cat# AAA124927)
Full Name
Anti-DDT Antibody
Gene Names
DDT; D-DT; DDCT; MIF2; MIF-2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of INPPL1 using anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 antibody.INPPL1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-INPPL1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
INPPL1, Polyclonal Antibody (Cat# AAA124934)
Full Name
Anti-INPPL1 Antibody
Gene Names
INPPL1; OPSMD; SHIP2
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of CD1b using anti-CD1b antibody.CD1b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD1b antibody.CD1b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD1b antibody.CD1b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD1b Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD1b, Polyclonal Antibody (Cat# AAA124937)
Full Name
Anti-CD1b Antibody
Gene Names
CD1B; R1; CD1; CD1A
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of UBE2I UBC9 using anti-UBE2I UBC9 antibody.UBE2I UBC9 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UBE2I UBC9 antibody.UBE2I UBC9 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UBE2I UBC9 antibody.UBE2I UBC9 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UBE2I UBC9 antibody.UBE2I UBC9 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UBE2I UBC9 antibody.UBE2I UBC9 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UBE2I UBC9 antibody.UBE2I UBC9 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UBE2I UBC9 antibody.UBE2I UBC9 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UBE2I UBC9 antibody.UBE2I UBC9 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-UBE2I UBC9 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
UBE2I UBC9, Polyclonal Antibody (Cat# AAA124939)
Full Name
Anti-UBE2I UBC9 Antibody
Gene Names
UBE2I; P18; UBC9; C358B7.1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of Caspase-2 using anti-Caspase-2 antibody.Caspase-2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase-2 antibody.Caspase-2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase-2 antibody.Caspase-2 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase-2 antibody.Caspase-2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase-2 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Caspase-2/CASP2, Polyclonal Antibody (Cat# AAA124940)
Full Name
Anti-Caspase-2/CASP2 Antibody
Gene Names
CASP2; ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of NSE using anti-NSE antibody.NSE was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NSE antibody.NSE was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NSE antibody.NSE was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NSE antibody.NSE was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NSE antibody.NSE was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NSE Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
NSE/ENO2, Polyclonal Antibody (Cat# AAA124946)
Full Name
Anti-NSE/ENO2 Antibody
Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. This ability to bind multiple epitopes is what makes polyclonal antibodies highly sensitive, as explained in our detailed guide on polyclonal antibodies and why they matter.
As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in our catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.