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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 3200-3250 of 10000 product results
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse heart cells using RYR2 Rabbit pAb (AAA10638) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
RYR2, Polyclonal Antibody (Cat# AAA10638)
Full Name
RYR2 Polyclonal Antibody
Gene Names
RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of HeLa cells using RPS12 Polyclonal Antibody (AAA10775) at dilution of 1:400. Blue: DAPI for nuclear staining.)
RPS12, Polyclonal Antibody (Cat# AAA10775)
Full Name
RPS12 Polyclonal Antibody
Gene Names
RPS12; S12
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of Lysates HeLa, using Phospho-CDK1-T14 Rabbit pAb (AAA10633) at 1:500 dilution.HeLa cells were treated by Hydroxyurea (4 mM) at 37 degree C for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
CDK1-T14, Polyclonal Antibody (Cat# AAA10633)
Full Name
Phospho-CDK1-T14 Polyclonal Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Applications
Western Blot
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using STAT3 Rabbit pAb (AAA10727) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
STAT3, Polyclonal Antibody (Cat# AAA10727)
Full Name
STAT3 Polyclonal Antibody
Gene Names
STAT3; APRF; HIES
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(AAA30993 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IGF1R, Polyclonal Antibody (Cat# AAA30993)
Full Name
Phospho-IGF1R (Tyr1346) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31187 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31187 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Syntrophin alpha 1, Polyclonal Antibody (Cat# AAA31187)
Full Name
Syntrophin alpha 1 Antibody
Gene Names
SNTA1; SNT1; LQT12; TACIP1; dJ1187J4.5
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Rabbit(88%), Dog(88%)
Predicted Reactivity: Rabbit(88%), Dog(88%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Figure 10 Immunofluorescence Validation of TACE in Rat Brain (Pradillo et al, 2005)Cellular localization of TACE. Double immunofluorescence staining of brain sections from sham-operated (SHAM; A, C, E) and IPC-exposed animals (IPC; B, D, F) of TACE (red) and the cellular markers (green) NeuN (neurons; A, B), GFAP (astrocytes; C, D) and L. esculentum lectin (microglia and endothelium; E, F). White arrows indicate TACE-positive cells.)
TACE, Polyclonal Antibody (Cat# AAA10912)
Full Name
TACE Antibody
Gene Names
ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
TACE Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Antibody (0.5 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
AIF1 / IBA1, Polyclonal Antibody (Cat# AAA12318)
Full Name
Goat Polyclonal to Human AIF1 / IBA1
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human, Monkey, Mouse, Rat, Hamster, Pig
Applications
Immunohistochemistry, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of LANCL2 in mouse brain tissue with LANCL2 antibody at 5 μg/mL.)
LANCL2, Polyclonal Antibody (Cat# AAA10978)
Full Name
LANCL2 Antibody
Gene Names
LANCL2; TASP; GPR69B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31263 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
UBR4, Polyclonal Antibody (Cat# AAA31263)
Full Name
UBR4 Antibody
Gene Names
UBR4; p600; ZUBR1; RBAF600
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Figure 12 Overexpression Validation in Spike Transfected 293 Cells Loading: 15 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike, AAA10931 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells.)
COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA10931)
Full Name
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody
Reactivity
Virus
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Western blot analysis of Albumin expression in human liver tissue lysate with Albumin antibody at 0.25 ug/ml.)
Albumin, Polyclonal Antibody (Cat# AAA10940)
Full Name
Albumin Antibody
Gene Names
ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Albumin Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Dystrophin Rabbit pAb (AAA10720) at dilution of 1:50 (40x lens).Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) ( at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.)
DMD, Polyclonal Antibody (Cat# AAA10720)
Full Name
DMD Polyclonal Antibody
Gene Names
DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse brain using MEGF10 antibody (AAA28229) at dilution of 1:100. Blue: DAPI for nuclear staining.)
MEGF10, Polyclonal Antibody (Cat# AAA28229)
Full Name
MEGF10 Polyclonal Antibody
Gene Names
MEGF10; EMARDD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using TFF1 antibody at dilution of 1:200 (40x lens).)
TFF1, Polyclonal Antibody (Cat# AAA10666)
Full Name
TFF1 Polyclonal Antibody
Gene Names
TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using GNA15 antibody (AAA10668) at dilution of 1:100 (40x lens).)
GNA15, Polyclonal Antibody (Cat# AAA10668)
Full Name
GNA15 Polyclonal Antibody
Gene Names
GNA15; GNA16
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(IHC Validation of Envelope in COVID-19 Patient Skin (Magro et al., 2020) Detection of SARS-CoV-2 Envelope protein in the blood vessels of COVID-19 patients that were confirmed by PCR. The staining shows Envelope protein expression (green) detected by envelope antibodies (AAA10932, 3 ug/mL) in the endothelial cytoplasms in thrombosed and normal appearing blood vessels with hematoxylin counterstain. The staining was negative in control normal skin/lung (not shown).)
COVID 19 Envelope Coronavirus, Polyclonal Antibody (Cat# AAA10932)
Full Name
SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody
Reactivity
Virus
Applications
Immunofluorescence, Immunohistochemistry
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
p38 gamma/delta, Polyclonal Antibody (Cat# AAA31407)
Full Name
Phospho-p38 gamma/delta (Tyr185/Tyr182) Antibody
Gene Names
MAPK12; ERK3; ERK6; SAPK3; PRKM12; SAPK-3; P38GAMMA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31126 at 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
MT2A, Polyclonal Antibody (Cat# AAA31126)
Full Name
MT2A Antibody
Gene Names
MT2A; MT2; MT-2; MT-II
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(AAA31005 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Tau, Polyclonal Antibody (Cat# AAA31005)
Full Name
Phospho-Tau (Ser262) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of THP-1 cells using CD11a/LFA-1A/ITGAL Rabbit pAb (AAA10672) at dilution of 1:200. Blue: DAPI for nuclear staining.)
ITGAL, Polyclonal Antibody (Cat# AAA10672)
Full Name
ITGAL Polyclonal Antibody
Gene Names
ITGAL; CD11A; LFA-1; LFA1A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Pricing
IF (Immuofluorescence)
(Figure 9 Immunofluorescence Validation of ACE2 In Caco2 Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with AAA10928 at 20 ug/mL,followed by goat anti-rabbit IgG secondary antibody at1/500 dilution (green) and DAPI staining (blue). Imageshowing membrane staining on Caco2 cells.)
ACE2, Polyclonal Antibody (Cat# AAA10928)
Full Name
ACE2 Antibody
Gene Names
ACE2; ACEH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
ACE2 Antibody is affinity chromatography purified via peptide column.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Cleaved-Caspase 1, Polyclonal Antibody (Cat# AAA31336)
Full Name
Cleaved-Caspase 1 (Ala317), p10 Antibody
Gene Names
CASP1; ICE; P45; IL1BC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Bovine Kidney Tissue;Primary Ab: 20ug/ml Rabbit Anti-Bovine IL1b AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))
Interleukin 1 Beta, Polyclonal Antibody (Cat# AAA20864)
Full Name
Polyclonal Antibody to Interleukin 1 Beta (IL1b)
Reactivity
Bovine
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using BRCA2 antibody (AAA10700) at dilution of 1:100. Blue: DAPI for nuclear staining.)
BRCA2, Polyclonal Antibody (Cat# AAA10700)
Full Name
BRCA2
Gene Names
BRCA2; FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
Reactivity
Human , Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Pricing
WB (Western Blot)
(PVRL4 Antibody (Center) (AAA28678) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PVRL4 antibody detected the PVRL4 protein (arrow).)
PVRL4, Polyclonal Antibody (Cat# AAA28678)
Full Name
PVRL4 Antibody (Center)
Gene Names
PVRL4; LNIR; PRR4; EDSS1; nectin-4
Reactivity
Human
Applications
Western Blot, Dot Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(AAA31063 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NF kappaB p65, Polyclonal Antibody (Cat# AAA31063)
Full Name
Phospho-NF kappaB p65 (Ser281) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using MT2A antibody at dilution of 1:200 (40x lens).)
MT2A, Polyclonal Antibody (Cat# AAA10681)
Full Name
MT2A Polyclonal Antibody
Gene Names
MT2A; MT2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using TYR antibody (AAA10704) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
TYR, Polyclonal Antibody (Cat# AAA10704)
Full Name
TYR Polyclonal Antibody
Gene Names
TYR; ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(AAA31118 staining 293T by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GPR133, Polyclonal Antibody (Cat# AAA31118)
Full Name
GPR133 Antibody
Gene Names
ADGRD1; PGR25; GPR133
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of MLZE expression in mouse spleen (A), rat kidney (B), rat spleen (C) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 58 kD))
MLZE, Polyclonal Antibody (Cat# AAA27810)
Full Name
Anti-MLZE Antibody
Gene Names
GSDMC; MLZE
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with TGF-beta (100ng/ml, 30 min), using Phospho-SMAD4 Antibody(upper) or Beta-actin (lower).)
Phospho-SMAD4 (T277), Polyclonal Antibody (Cat# AAA28715)
Full Name
Phospho-SMAD4 (T277) Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse
Predicted Species Reactivity: Rat
Predicted Species Reactivity: Rat
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31240 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31240) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Anillin, Polyclonal Antibody (Cat# AAA31240)
Full Name
Anillin Antibody
Gene Names
ANLN; scra; FSGS8; Scraps
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-FAK (Tyr576) Antibody expression in NIH-3T3 cells lysates.The lane on the right is treated with the antigen-specific peptide.)
FAK, Polyclonal Antibody (Cat# AAA31066)
Full Name
Phospho-FAK (Tyr576) Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(DDR1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
DDR1, Polyclonal Antibody (Cat# AAA28772)
Full Name
DDR1 Antibody (Center)
Gene Names
DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(AAA31262 staining H2O2 treated Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31262) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
VGluT1, Polyclonal Antibody (Cat# AAA31262)
Full Name
VGluT1 Antibody
Gene Names
SLC17A7; BNPI; VGLUT1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Rabbit(80%), Dog(80%)
Predicted Reactivity: Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Rabbit(80%), Dog(80%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Kidney Tissue)
Integrin Beta 3 (ITGb3), Polyclonal Antibody (Cat# AAA20173)
Full Name
Polyclonal Antibody to Integrin Beta 3 (ITGb3)
Gene Names
ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Rat testis using LETMD1 Rabbit pAb (AAA10722) at dilution of 1:100 (40x lens).)
LETMD1, Polyclonal Antibody (Cat# AAA10722)
Full Name
LETMD1 Polyclonal Antibody
Gene Names
LETMD1; HCCR1; HCCR-1; HCCR-2; HCRR-2; 1110019O13Rik
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
FAK, Polyclonal Antibody (Cat# AAA31446)
Full Name
Phospho-FAK (Ser722) Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
ALDOSTERONE, Polyclonal Antibody (Cat# AAA12239)
Full Name
RABBIT ANTI ALDOSTERONE
Applications
Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cellsusing Fatty Acid Synthase antibody(AAA10626). Blue: DAPI for nuclear staining)
Fatty Acid Synthase, Polyclonal Antibody (Cat# AAA10626)
Full Name
FASN Polyclonal Antibody
Gene Names
FASN; FAS; OA-519; SDR27X1
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Figure 3. IF analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody PC1/3/PCSK1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight 488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
PC1/3, Polyclonal Antibody (Cat# AAA11585)
Full Name
Anti-PC1/3 antibody
Gene Names
PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12
Reactivity
Reacts with: Human, rat; Predicted to work with: Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA26913 at dilution of 1:100)
CTPS1, Polyclonal Antibody (Cat# AAA26913)
Full Name
CTPS1 Antibody
Gene Names
CTPS1; CTPS; IMD24
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(AAA31122 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31122) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
IL-6, Polyclonal Antibody (Cat# AAA31122)
Full Name
IL-6 Antibody
Gene Names
IL6; CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31134 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA31134) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
PERK, Polyclonal Antibody (Cat# AAA31134)
Full Name
Phospho-PERK (Thr982) Antibody
Gene Names
EIF2AK3; PEK; WRS; PERK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Peptide ELISA
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Rat Intestine Tissue;Primary Ab: 20ug/ml Rabbit Anti-Rat PANX1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))
Pannexin 1 (PANX1), Polyclonal Antibody (Cat# AAA20074)
Full Name
Polyclonal Antibody to Pannexin 1 (PANX1)
Gene Names
Panx1; px1
Reactivity
Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cells using AHR Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining)
AHR, Polyclonal Antibody (Cat# AAA10754)
Full Name
AHR Polyclonal Antibody
Gene Names
AHR; bHLHe76
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purification
Pricing
QC (Quality Control)
Treponema pallidum, Polyclonal Antibody (Cat# AAA13577)
Full Name
Treponema pallidum
Reactivity
Eubacteria
Applications
Immunohistochemistry
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
alpha Tubulin, Polyclonal Antibody (Cat# AAA31344)
Full Name
Acetyl-alpha Tubulin (Lys40) Antibody
Gene Names
TUBA1B; K-ALPHA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using DiMethyl-Histone H3-K4 Rabbit pAb (AAA10651) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
H3K4me2, Polyclonal Antibody (Cat# AAA10651)
Full Name
Histone H3K4me2 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.