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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 3150-3176 of 3176 product results
Mycoplasma hyopneumoniae 46 kDa surface antigen, Polyclonal Antibody (Cat# AAA18877)
NDI1, Polyclonal Antibody (Cat# AAA27939)
Thyroxine, Polyclonal Antibody (Cat# AAA14321)
Rabies virus Nucleoprotein, Polyclonal Antibody (Cat# AAA18817)
collagen, type I, alpha 2, Polyclonal Antibody (Cat# AAA15910)
OLFR734, Polyclonal Antibody (Cat# AAA27943)
Hepatitis C Virus, Polyclonal Antibody (Cat# AAA14328)
alpha 1-Acid Glycoprotein, Polyclonal Antibody (Cat# AAA14445)
dnaK, Polyclonal Antibody (Cat# AAA26989)
ATP1A3, Polyclonal Antibody (Cat# AAA26972)
Diazepam Binding Inhibitor (DBI), Polyclonal Antibody (Cat# AAA20156)
Rotavirus A Outer capsid protein VP4, Polyclonal Antibody (Cat# AAA18867)
K2, Polyclonal Antibody (Cat# AAA14536)
WB (Western Blot)
(Western Blot: Sample: Recombinant F8 protein.)
Coagulation Factor VIII (F8), Polyclonal Antibody (Cat# AAA20084)
ESAT-6, Polyclonal Antibody (Cat# AAA20027)
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26882)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using VAPB antibody. Blue: DAPI for nuclear staining.)
VAPB, Polyclonal Antibody (Cat# AAA28162)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat brain using SCAF4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
SCAF4, Polyclonal Antibody (Cat# AAA28418)
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of 293T cells using anti-MYNN antibody (AAA19970).Overlay histogram showing 293T cells stained with AAA19970 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYNN Antibody (AAA19970, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MYNN, Polyclonal Antibody (Cat# AAA19970)
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
HDC, Polyclonal Antibody (Cat# AAA29186)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with CDk1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
CDk1, Polyclonal Antibody (Cat# AAA29954)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
IRS-1, Polyclonal Antibody (Cat# AAA28930)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cell using HLA-A antibody. Blue: DAPI for nuclear staining)
HLA-A, Polyclonal Antibody (Cat# AAA29698)
WB (Western Blot)
Cytokeratin 20/Krt20, Polyclonal Antibody (Cat# AAA19202)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
NDUFB8, Polyclonal Antibody (Cat# AAA22274)
WB (Western Blot)
(WB Suggested Anti-TRAK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HT1080 cell lysateThere is BioGPS gene expression data showing that TRAK1 is expressed in HT1080)
TRAK1, Polyclonal Antibody (Cat# AAA23554)
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.