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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 3300-3350 of 10000 product results
IF (Immunofluorescence)
(AAA31249 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31249 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Amino-terminal enhancer of split, Polyclonal Antibody (Cat# AAA31249)
Full Name
Amino-terminal enhancer of split Antibody
Gene Names
TLE5; AES; GRG; ESP1; GRG5; AES-1; AES-2; Grg-5
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Xenopus(86%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Xenopus(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31251 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Dcp2, Polyclonal Antibody (Cat# AAA31251)
Full Name
Dcp2 Antibody
Gene Names
DCP2; NUDT20
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunoflouorescence)
(AAA31011 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
ATF2, Polyclonal Antibody (Cat# AAA31011)
Full Name
Phospho-ATF2 (Thr69/51) Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31269 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Snx6, Polyclonal Antibody (Cat# AAA31269)
Full Name
Snx6 Antibody
Gene Names
SNX6; TFAF2; MSTP010
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry-Paraffin)
(Immunochemical staining CD21 in cynomolgus lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
CD21, Polyclonal Antibody (Cat# AAA27778)
Full Name
Anti-CD21 Antibody, Rabbit Polyclonal
Reactivity
Human, Cynomolgus
(Species predicted to react based on 100% sequence homology)
(Species predicted to react based on 100% sequence homology)
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Pricing
FCM (Flow Cytometry)
(CCR7 Antibody (N-term) (AAA28701) flow cytometric analysis of 293 cells (right histogram) compared to a negative controlcell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CCR7, Polyclonal Antibody (Cat# AAA28701)
Full Name
CCR7 Antibody (N-term)
Gene Names
CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast cancer using KIF22 antibody (AAA28282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol)
KIF22, Polyclonal Antibody (Cat# AAA28282)
Full Name
KIF22 Polyclonal Antibody
Gene Names
KIF22; KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(LTF Antibody flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (lefthistogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for theanalysis.)
LTF, Polyclonal Antibody (Cat# AAA28680)
Full Name
LTF Antibody
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Reactivity
Human, mouse
Applications
Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(MUSK Antibody (AAA28697) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
MUSK, Polyclonal Antibody (Cat# AAA28697)
Full Name
MUSK Antibody
Gene Names
MUSK; CMS9
Reactivity
Human, mouse
Applications
Western Blot, Flow Cytometry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (PAB)
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using AAA29717 at dilution 1/5.)
CLEC7A, Polyclonal Antibody (Cat# AAA29717)
Full Name
CLEC7A Antibody
Gene Names
CLEC7A; BGR; CD369; CANDF4; DECTIN1; CLECSF12
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Skin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human F12 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))
Prothrombin Fragment 1+2 (F1+2), Polyclonal Antibody (Cat# AAA20072)
Full Name
Polyclonal Antibody to Prothrombin Fragment 1+2 (F1+2)
Gene Names
F2; PT; THPH1; RPRGL2
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(Western blot analysis of extracts from A431, using Phospho-SHP-2 (Tyr62) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
SHP-2, Polyclonal Antibody (Cat# AAA31155)
Full Name
Phospho-SHP-2 (Tyr62) Antibody
Gene Names
PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31156 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31156 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
Beclin-1, Polyclonal Antibody (Cat# AAA31156)
Full Name
Phospho-Beclin-1 (Ser90/93/96) Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31217 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
TBLR1, Polyclonal Antibody (Cat# AAA31217)
Full Name
TBLR1 Antibody
Gene Names
TBL1XR1; C21; DC42; IRA1; MRD41; TBLR1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffinembedded mouse brain using KISS1R Antibody (AAA10717) at dilution of 1:100 (40x lens).)
KISS1R, Polyclonal Antibody (Cat# AAA10717)
Full Name
KISS1R Polyclonal Antibody
Gene Names
KISS1R; HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(AAA30956 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA30956 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
MITF, Polyclonal Antibody (Cat# AAA30956)
Full Name
Phospho-MITF (Ser180/73) Antibody
Gene Names
MITF; MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity puriification
Pricing
Application Data
(The cancer cell lines were stably expressing DYKDDDDK-tagged RIP3 and RIP expression was detected by anti-RIP3 antibodies (AAA10922) in RIP3-overexpressed cells.)
RIP3, Polyclonal Antibody (Cat# AAA10922)
Full Name
RIP3 Antibody
Gene Names
Ripk3; Rip3; AW107945; 2610528K09Rik
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
RIP3 Antibody is affinity chromatography purified via peptide column.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28788 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28788, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
IDUA, Polyclonal Antibody (Cat# AAA28788)
Full Name
IDUA Antibody (Center)
Gene Names
IDUA; IDA; MPS1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
(Jia, Wei?Qiang, et al. "Verbascoside inhibits progression of glioblastoma cells by promoting Let?7g?5p and down?regulating HMGA2 via Wnt/beta?catenin signalling blockade." Journal of cellular and molecular medicine 24.5 (2020): 2901-2916.)
LC3B, Polyclonal Antibody (Cat# AAA30772)
Full Name
LC3B Rabbit Polyclonal Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistchemistry)
(AAA30928 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Nrf2, Polyclonal Antibody (Cat# AAA30928)
Full Name
Nrf2 Antibody
Gene Names
NFE2L2; NRF2; HEBP1; IMDDHH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
SREBP-1c, Polyclonal Antibody (Cat# AAA27927)
Full Name
SREBP-1c Antibody
Gene Names
SREBF1; SREBP1; bHLHd1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Filaggrin staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Filaggrin, Polyclonal Antibody (Cat# AAA27815)
Full Name
Anti-Filaggrin Antibody
Gene Names
FLG; ATOD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(AAA31014 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Histone H2A.X, Polyclonal Antibody (Cat# AAA31014)
Full Name
Phospho-Histone H2A.X (Ser139) Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA26952 at 1:333, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
XKR8, Polyclonal Antibody (Cat# AAA26952)
Full Name
XKR8 Antibody
Gene Names
XKR8; XRG8; hXkr8
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of ZNT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
ZNT1, Polyclonal Antibody (Cat# AAA27809)
Full Name
Anti-ZNT1 Antibody
Gene Names
SLC30A1; ZNT1; ZRC1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of lysate from HT-29 cell line, using ERN2 Antibody (N-term). AAA28749 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
ERN2, Polyclonal Antibody (Cat# AAA28749)
Full Name
ERN2 Antibody (N-term)
Gene Names
ERN2; IRE1b; IRE2p; hIRE2p; IRE1-BETA
Reactivity
Human
Applications
Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Western Blot;Sample: Mouse Small intestine lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse DEFa1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Western Blot))
Defensin Alpha 1, Polyclonal Antibody (Cat# AAA20957)
Full Name
Polyclonal Antibody to Defensin Alpha 1, Neutrophil (DEFa1)
Gene Names
Defa1; Defcr; Defcr1
Reactivity
Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31243 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ADCY10, Polyclonal Antibody (Cat# AAA31243)
Full Name
ADCY10 Antibody
Gene Names
ADCY10; SAC; HCA2; SACI; Sacy; hsAC; HEL-S-7a
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-PSamples: Mouse Lung TissuePrimary Ab: 10ug/ml Rabbit Anti-MousePLA2R1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Immunohistochemistry))
Phospholipase A2 Receptor 1, Polyclonal Antibody (Cat# AAA20865)
Full Name
Polyclonal Antibody to Phospholipase A2 Receptor 1 (PLA2R1)
Gene Names
Pla2r1; Pla2r; Pla2-r; Pla2g1br
Reactivity
Mouse
Applications
WB, IHC, ICC, IP
Purity
Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
Azurocidin, Polyclonal Antibody (Cat# AAA14707)
Full Name
Azurocidin, Human (CAP37, HBP)
Gene Names
AZU1; AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
Reactivity
Human
Applications
Western Blot, Immunoelectrophoresis
Purity
Purified by ammonium sulfate precipitation.
Pricing
SDS-PAGE
(Figure 2. 10% SDS-PAGE, purified rabbit IgG of anti-M protein (2019-nCoV) antibody (AAA13777))
COVID 19 Membrane Protein (M) Coronavirus, Polyclonal Antibody (Cat# AAA13777)
Full Name
Anti-M (2019-nCoV) Rabbit Polyclonal Antibody
Applications
Western Blot
Purity
Protein G Chromatography
Pricing
Application Data
(The indicated His-PGK1 proteins immobilized on Ni-NTAagarose beads was incubated with DYKDDDDK-ARD1 and Ac-CoA, followed by incubation with purified GST-Beclin1 or GST-Beclin1 S30A, inthe presence of [γ-32P] ATP. Autoradiography was performed.)
Beclin1 (Phospho-Ser30), Polyclonal Antibody (Cat# AAA29760)
Full Name
Beclin1 (Phospho-Ser30) Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue AAA29660 using at dilution 1/40.)
PRKD1, Polyclonal Antibody (Cat# AAA29660)
Full Name
PRKD1 Antibody
Gene Names
PRKD1; PKD; PKCM; PRKCM; PKC-MU
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of FKHRL1 phosphorylation expression in serum treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
FKHRL1, Polyclonal Antibody (Cat# AAA30954)
Full Name
Phospho-FKHRL1 (Ser253) Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31110 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
CHSY1, Polyclonal Antibody (Cat# AAA31110)
Full Name
CHSY1 Antibody
Gene Names
CHSY1; CHSY; CSS1; TPBS; ChSy-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31197 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31197 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CXCL16, Polyclonal Antibody (Cat# AAA31197)
Full Name
CXCL16 Antibody
Gene Names
CXCL16; SRPSOX; CXCLG16; SR-PSOX
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(HA1(A/Brisbane/10/2007) recombinant protein)
H3(H3N2)(A/Brisbane/10/2007), Polyclonal Antibody (Cat# AAA13729)
Full Name
Anti-H3(H3N2)(A/Brisbane/10/2007)
Reactivity
Cross reactivity to other subtypes not tested
Applications
Immunoprecipitation
Purity
Immunoaffinity chromatography
Pricing
WB (Western Blot)
(WB of AAA14403 with 1:500 antibody dilution in DiluObuffer. Apparent MW: 48)
FtsZ, Polyclonal Antibody (Cat# AAA14403)
Full Name
FtsZ Antibody
Applications
Immunoprecipitation, Western Blot
Pricing
WB (Western Blot)
GPCR GPR43 / FFAR2, Polyclonal Antibody (Cat# AAA14408)
Full Name
GPCR GPR43 Antibody
Gene Names
FFAR2; FFA2R; GPR43
Reactivity
Human, Mouse, Rat
Applications
Dot Blot, Immunohistochemistry, Immunoprecipitation, Western Blot
Pricing
WB (Western Blot)
(Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). AAA28778 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
LC3, Polyclonal Antibody (Cat# AAA28778)
Full Name
LC3 Antibody (APG8B) (N-term)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, rat (Predicted Reactivity: Bovine)
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry-Paraffin)
(Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Selenium Binding Protein 1, Polyclonal Antibody (Cat# AAA28823)
Full Name
Selenium Binding Protein 1 Polyclonal Antibody
Gene Names
SELENBP1; LPSB; SP56; hSBP; SBP56; HEL-S-134P
Reactivity
Rat, Dog, Cow
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Collagen 4 alpha 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Collagen 4 alpha 5, Polyclonal Antibody (Cat# AAA27790)
Full Name
Anti-Collagen 4 alpha 5 Antibody
Gene Names
COL4A5; ATS; ASLN; CA54
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-PDE7A (bs-11575R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 55kD)
PDE7A, Polyclonal Antibody (Cat# AAA28812)
Full Name
PDE7A Polyclonal Antibody
Gene Names
PDE7A; HCP1; PDE7; PDE7A
Reactivity
Rat, Pig, Dog, Cow
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(AAA29758 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
STING, Polyclonal Antibody (Cat# AAA29758)
Full Name
STING (Phospho-Ser366) Antibody
Gene Names
TMEM173; ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING
Reactivity
Human, Mouse
Applications
Westen Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA13663 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
P2RX7/P2X7 receptor, Polyclonal Antibody (Cat# AAA13663)
Full Name
Goat anti-P2RX7/P2X7 receptor Antibody
Gene Names
P2RX7; P2X7
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
MOR1, Polyclonal Antibody (Cat# AAA14412)
Full Name
MOR1 Antibody
Gene Names
OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Pricing
WB (Western Blot)
(Western blot analysis of JunD phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
JunD, Polyclonal Antibody (Cat# AAA31019)
Full Name
Phospho-JunD (Ser255) Antibody
Gene Names
JUND; AP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31224 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31224 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CASC5, Polyclonal Antibody (Cat# AAA31224)
Full Name
CASC5 Antibody
Gene Names
KNL1; D40; CT29; Spc7; CASC5; MCPH4; hKNL-1; AF15Q14; PPP1R55; hSpc105
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(86%)
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(HMGB1 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- HMGB1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
HMGB1, Polyclonal Antibody (Cat# AAA11677)
Full Name
Anti-HMGB1 Antibody
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence – anti-CD63 Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA;)
CD63, Polyclonal Antibody (Cat# AAA13873)
Full Name
CD63 Polyclonal Antibody
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Reacts against human, rat, mouse, canine and monkey proteins
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.