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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 3350-3400 of 10000 product results
WB (Western Blot)
(Western blot analysis of EGFR phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
EGFR, Polyclonal Antibody (Cat# AAA30962)
Full Name
Phospho-EGFR (Ser695) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31276 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SCAMP2, Polyclonal Antibody (Cat# AAA31276)
Full Name
SCAMP2 Antibody
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(HMGB1 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- HMGB1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
HMGB1, Polyclonal Antibody (Cat# AAA11677)
Full Name
Anti-HMGB1 Antibody
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence – anti-CD63 Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA;)
CD63, Polyclonal Antibody (Cat# AAA13873)
Full Name
CD63 Polyclonal Antibody
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Reacts against human, rat, mouse, canine and monkey proteins
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA26950 at dilution of 1:100)
FLVCR2, Polyclonal Antibody (Cat# AAA26950)
Full Name
FLVCR2 Antibody
Gene Names
FLVCR2; CCT; EPV; PVHH; MFSD7C; C14orf58; FLVCRL14q
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence Validation of cIAP in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehydefixed human lung tissue abeling cIAP with 3325 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).)
cIAP, Polyclonal Antibody (Cat# AAA10954)
Full Name
cIAP Antibody
Gene Names
BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
cIAP Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human small intestine using AAA26955 at dilution 1:100)
PPM1D, Polyclonal Antibody (Cat# AAA26955)
Full Name
PPM1D Antibody
Gene Names
PPM1D; WIP1; PP2C-DELTA
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
WB (Western Blot)
(SLCO1B3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SLCO1B3 antibody detected the SLCO1B3 protein (arrow).)
SLCO1B3, Polyclonal Antibody (Cat# AAA28785)
Full Name
SLCO1B3 Antibody (C-term)
Gene Names
SLCO1B3; LST3; HBLRR; LST-2; OATP8; OATP-8; OATP1B3; SLC21A8; LST-3TM13
Reactivity
Human
Applications
Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) expression in various lysates)
PI3K p85 alpha, Polyclonal Antibody (Cat# AAA31027)
Full Name
Phospho-PI3K p85 alpha (Tyr607) Antibody
Gene Names
PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
Reactivity
Human, Mouse, Rat, Pig
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of Adrenergic Receptor beta2 phosphorylation expression in nocodazole treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Adrenergic Receptor beta2, Polyclonal Antibody (Cat# AAA30989)
Full Name
Phospho-Adrenergic Receptor beta2 (Ser346) Antibody
Gene Names
ADRB2; BAR; B2AR; ADRBR; ADRB2R; BETA2AR
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31196 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31196) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
PRDM16, Polyclonal Antibody (Cat# AAA31196)
Full Name
PRDM16 Antibody
Gene Names
PRDM16; MEL1; KMT8F; LVNC8; PFM13; CMD1LL
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of RPL22/L1 using K562 whole cell lysates)
RPL22/L1, Polyclonal Antibody (Cat# AAA31142)
Full Name
RPL22/L1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31206 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31206) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Activin Receptor Type IA, Polyclonal Antibody (Cat# AAA31206)
Full Name
Activin Receptor Type IA Antibody
Gene Names
ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31208 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SV2A, Polyclonal Antibody (Cat# AAA31208)
Full Name
SV2A Antibody
Gene Names
SV2A; SV2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Applications
ELISA
Purity
Peptide affinity purification
Pricing
KK-LC-1, Polyclonal Antibody (Cat# AAA14410)
Full Name
Cancer/testis antigen 83 antibody; Chromosome X open reading frame 61 antibody; CT83 antibody; CXorf61 antibody; Kita-kyushu lung cancer antigen 1 antibody; KKLC1 antibody
Gene Names
CT83; KKLC1; CXorf61; KK-LC-1
Applications
Immunoprecipitation, Western Blot
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG-2 cells using AAA26914 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
ZNRF3, Polyclonal Antibody (Cat# AAA26914)
Full Name
ZNRF3 Antibody
Gene Names
ZNRF3; RNF203; BK747E2.3
Reactivity
Human
Applications
Immunofluorescence
Purity
>95%, Protein G purified
Pricing
WB (Western Blot)
(Positive WB detected in Recombinant proteinAll lanes: gB antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43.3 kDaObserved band size: 46 kDa)
gB, Polyclonal Antibody (Cat# AAA27058)
Full Name
Rabbit anti-Epstein-Barr virus (strain B95-8)(HHV-4)(Human herpesvirus 4) gB Polyclonal Antibody
Reactivity
Epstein-Barr virus
Applications
Western Blot
Purity
Protein G
Pricing
FCM (Flow Cytometry)
(BDKRB1 Antibody (Center) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
BDK_1, Polyclonal Antibody (Cat# AAA28760)
Full Name
BDK_1 Antibody (Center)
Gene Names
BDKRB1; B1R; BKR1; B1BKR; BKB1R; BDKRB2; BRADYB1
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of AmCyan recombinant protein, using AAA29659 diluted at 1:5,000.)
AmCyan, Polyclonal Antibody (Cat# AAA29659)
Full Name
AmCyan Rabbit Polyclonal Antibody
Applications
Western Blot
Purity
Affinity purification using immunogen.
Pricing
IHC (Immunohistochemistry)
(AAA31083 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 92)
EGFR, Polyclonal Antibody (Cat# AAA31083)
Full Name
EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistochemistry)
(AAA30985 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Keratin 8, Polyclonal Antibody (Cat# AAA30985)
Full Name
Phospho-Keratin 8 (Ser432) Antibody
Gene Names
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of lung1 lysate using FGF10 antibody. The lane on the left is treated with the antigen-specific peptide.)
FGF10, Polyclonal Antibody (Cat# AAA31123)
Full Name
FGF10 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of various lysates, using TIPARP Rabbit pAb (AAA28273) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
TIPARP, Polyclonal Antibody (Cat# AAA28273)
Full Name
TIPARP Polyclonal Antibody
Gene Names
TIPARP; PARP7; ARTD14; pART14
Applications
Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western Blot analysis of SARS-CoV-2 Nucleocapsid Protein: Anti- SARS-CoV-2 Nucleocapsid Protein (AAA14886) was used at 2 µg/ml on (1) SARS-CoV-2 virus infected Vero Cell lysate and (2) Mock infected lysate.)
COVID 19 Nucleocapsid (NP) Coronavirus, Polyclonal Antibody (Cat# AAA14886)
Full Name
Coronavirus (COVID-19) Nucleocapsid Antibody
Applications
Western Blot
Purity
Protein A Chromatography
Pricing
Cardiolipin, Polyclonal Antibody (Cat# AAA14711)
Full Name
Cardiolipin
Reactivity
Human
Applications
ELISA
Purity
Purified by delipidation, defibrination and ammonium sulfate precipitation.
Pricing
IF (Immunofluorescence)
(Figure 15. IF analysis of ARHGEF7 using anti-ARHGEF7 antibody (AAA19780).ARHGEF7 was detected in a paraffin-embedded section of human prostste cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-ARHGEF7 Antibody (AAA19780) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
ARHGEF7, Polyclonal Antibody (Cat# AAA19780)
Full Name
Anti-ARHGEF7 Antibody Picoband
Gene Names
ARHGEF7; P50; P85; PAK3; PIXB; COOL1; P50BP; COOL-1; P85SPR; BETA-PIX; P85COOL1; Nbla10314
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(MCOLN1 Antibody (C-term) (AAA28690)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCOLN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
MCOLN1, Polyclonal Antibody (Cat# AAA28690)
Full Name
MCOLN1 Antibody (C-term)
Gene Names
MCOLN1; ML4; MG-2; MLIV; MST080; TRPML1; MSTP080; TRP-ML1; TRPM-L1
Reactivity
Human
Predicted Reactivity: Monkey, Mouse
Predicted Reactivity: Monkey, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31071 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
HER4, Polyclonal Antibody (Cat# AAA31071)
Full Name
Phospho-HER4 (Tyr1284) Antibody
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of lysate from A431 cell line, using SPRR2A Antibody (C-term). AAA28741 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
SPRR2A, Polyclonal Antibody (Cat# AAA28741)
Full Name
SPRR2A Antibody (C-term)
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
SYVN1 (HRD1), Polyclonal Antibody (Cat# AAA28764)
Full Name
SYVN1 (HRD1) Antibody (C-term)
Gene Names
SYVN1; DER3; HRD1
Reactivity
Human, mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Taurine Transporter staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Taurine Transporter, Polyclonal Antibody (Cat# AAA27812)
Full Name
Anti-Taurine Transporter Antibody
Gene Names
SLC6A6; TAUT
Reactivity
Human, Mouse, Rat, Dog
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PRC1, Polyclonal Antibody (Cat# AAA31450)
Full Name
Phospho-PRC1 (Thr481) Antibody
Gene Names
PRC1; ASE1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (80%), Horse (90%)
Predicted Reactivity: Pig (80%), Horse (90%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(AAA31369 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31369 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
FOXM1, Polyclonal Antibody (Cat# AAA31369)
Full Name
Phospho-FOXM1 (Ser35) Antibody
Gene Names
FOXM1; MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31216 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31216) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
FKBP25, Polyclonal Antibody (Cat# AAA31216)
Full Name
FKBP25 Antibody
Gene Names
FKBP3; FKBP-3; FKBP25; PPIase; FKBP-25
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
ROBO1, Polyclonal Antibody (Cat# AAA12322)
Full Name
Goat Polyclonal to Human ROBO1
Gene Names
ROBO1; SAX3; DUTT1
Reactivity
Bat, Bovine, Chicken, Dog, Xenopus, Hamster, Horse, Pig, Human, Monkey, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
WB (Western Blot)
(Western Blot:C: 293 cell extract controlE: 293 cell expressing p27 (SIV/mac239))
p27 (SIV/mac239), Polyclonal Antibody (Cat# AAA13724)
Full Name
Anti-p27 (SIV/mac239), rabbit IgG
Applications
Western Blot
Purity
Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Western Blot:1: 293 cell extract control2: 293 cell expressing Pol (SIV/mac239))
Pol (SIV/mac239), Polyclonal Antibody (Cat# AAA13725)
Full Name
Anti-Pol (SIV/mac239), rabbit IgG
Reactivity
Cross-reactivity to other subtypes not tested.
Applications
Western Blot
Purity
Immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Host: Rabbit Target Name: FOSL1 Sample Tissue: Human HepG2 Whole Cell Antibody Dilution: 1ug/ml)
FOSL1, Polyclonal Antibody (Cat# AAA23383)
Full Name
FOSL1 antibody - middle region
Gene Names
FOSL1; FRA; FRA1; fra-1
Reactivity
Tested Species Reactivity Human, Rat
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(AAA30986 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Fyn, Polyclonal Antibody (Cat# AAA30986)
Full Name
Phospho-Fyn (Tyr530) Antibody
Gene Names
FYN; SLK; SYN; p59-FYN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western Blot of KK-LC-1 Antibody (AAA14411) with: 1) THP1 cells2)THP1 cells treated with LPS1:250 antibody dilution in DiluOBufferTM)
KK-LC-1, Polyclonal Antibody (Cat# AAA14411)
Full Name
KK-LC-1 Antibody
Gene Names
CT83; KKLC1; CXorf61; KK-LC-1
Reactivity
Human, Monkey
Applications
Immunoprecipitation, Western Blot
Pricing
IF (Immunofluorescence)
(Immunofluorescence of LOVO cells, using CHRM4 Antibody. The picture on the right is treated with the synthesized peptide.)
CHRM4 / M4, Polyclonal Antibody (Cat# AAA12369)
Full Name
Rabbit Polyclonal (IgG) to Human CHRM4 / M4
Gene Names
CHRM4; HM4; M4R
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Peptide ELISA
Purity
Immunoaffinity Purified
Pricing
Application Data
c-Src (Tyr-215), Polyclonal Antibody (Cat# AAA14100)
Full Name
c-Src (Tyr-215), phospho-specific [Conserved site]
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, Rat, Mouse
Applications
Western Blot
Pricing
IHC (Immunohistochemistry)
(Anti- S100A9 Picoband antibody, AAA11645, IHC(P)IHC(P): Rat Spleen Tissue)
S100A9, Polyclonal Antibody (Cat# AAA11645)
Full Name
Anti-S100A9 Antibody
Gene Names
S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using cGAS Rabbit pAb (AAA28207) at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
MB21D1, Polyclonal Antibody (Cat# AAA28207)
Full Name
MB21D1 Polyclonal Antibody
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.)
NANOG, Polyclonal Antibody (Cat# AAA28706)
Full Name
NANOG Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
PPAR-alpha, Polyclonal Antibody (Cat# AAA29102)
Full Name
PPAR-alpha Polyclonal Antibody
Gene Names
PPARA; PPAR; NR1C1; hPPAR; PPARalpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Pricing
IF (Immunofluorescence)
(AAA31204 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31204 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
MSH4, Polyclonal Antibody (Cat# AAA31204)
Full Name
MSH4 Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%)
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(All lanes : Anti-TIGIT Antibody at 1:2000 dilutionLane 1: EL4 whole cell lysateLane 2: human fetal thymus lysateLane 3: human spleen lysateLane 4: HUT78 whole cell lysateLane 5: Jurkat whole cell lysateLane 6: MOLT-4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
TIGIT, Polyclonal Antibody (Cat# AAA28803)
Full Name
TIGIT Antibody
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human, Mouse
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of PGD2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
PGD2 Receptor, Polyclonal Antibody (Cat# AAA27807)
Full Name
Anti-PGD2 Receptor Antibody
Gene Names
PTGDR; DP; AS1; DP1; ASRT1; PTGDR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.