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WB (Western Blot)
(Western Blot analysis of extracts from K562 cells, using AAT Polyclonal Antibody. Secondary antibody was diluted at 1:20000)
Rabbit AAT Polyclonal Antibody | anti-AAT antibody
AAT Rabbit Polyclonal Antibody
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
AAT; Polyclonal Antibody; AAT Rabbit Polyclonal Antibody; SERPINA1; PI; Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1; BH6842; anti-AAT antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
AAT Polyclonal Antibody detects endogenous levels of AAT protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-AAT antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
IHC-p: 1:100-300
ELISA: 1/40000
IHC-p: 1:100-300
ELISA: 1/40000
Immunogen
Synthesized peptide derived from AAT. at AA range: 240-320
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
WB (Western Blot)
(Western Blot analysis of extracts from K562 cells, using AAT Polyclonal Antibody. Secondary antibody was diluted at 1:20000)
WB (Western Blot)
(Western Blot analysis of extracts from K562 cells, using AAT Polyclonal Antibody. Secondary antibody was diluted at 1:20000)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Related Product Information for anti-AAT antibody
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,737 Da
NCBI Official Full Name
alpha-1-antitrypsin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
NCBI Official Symbol
SERPINA1
NCBI Official Synonym Symbols
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
NCBI Protein Information
alpha-1-antitrypsin; serpin A1; alpha-1-antiproteinase; alpha-1-antitrypsin null; alpha-1 protease inhibitor; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1
UniProt Protein Name
Alpha-1-antitrypsin
UniProt Gene Name
SERPINA1
UniProt Synonym Gene Names
AAT; PI; SPAAT
UniProt Entry Name
A1AT_HUMAN
Similar Products
Product Notes
The AAT serpina1 (Catalog #AAA30751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AAT Rabbit Polyclonal Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AAT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA). WB: 1/500-1/2000 IHC-p: 1:100-300 ELISA: 1/40000. Researchers should empirically determine the suitability of the AAT serpina1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AAT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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