Rabbit ACK1 Polyclonal Antibody | anti-TNK2 antibody

ACK1 Antibody (N-term)

Cat. Num. AAA285032

• Gene Names: TNK2; ACK; ACK1; ACK-1; p21cdc42Hs
• Reactivity: Human (Predicted Reactivity: Bovine)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ACK1, Antibody; ACK1 Antibody (N-term); Activated CDC42 kinase 1; ACK-1; Tyrosine kinase non-receptor protein 2; TNK2; ACK1; anti-TNK2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human ACK1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 10-39
• Sequence Length: 1,086

• Applicable Applications for anti-TNK2 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of NCI-H292 cells using ACK1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with ACK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).)

WB (Western Blot)

(Western blot analysis of ACK1 Antibody (N-term) in K562 cell line lysates (35ug/lane). ACK1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-TNK2 antibody

ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Product Categories/Family for anti-TNK2 antibody

Cancer; Signal Transduction

References

Yokoyama, N., et al., J. Biol. Chem. 278(48):47713-47723 (2003).
Manser, E., et al., Nature 363(6427):364-367 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 58331191
• NCBI GeneID: 10188
• NCBI Accession #: NP_001010938.1; NP_005772.3
• NCBI GenBank Nucleotide #: NM_001010938.1
• UniProt Accession #: Q07912; Q6ZMQ0; Q8N6U7; Q96H59
• Molecular Weight: 114569
• NCBI Official Full Name: activated CDC42 kinase 1 isoform 2
• NCBI Official Synonym Full Names: tyrosine kinase, non-receptor, 2
• NCBI Official Symbol: TNK2
• NCBI Official Synonym Symbols: ACK; ACK1; ACK-1; p21cdc42Hs
• NCBI Protein Information: activated CDC42 kinase 1
• UniProt Protein Name: Activated CDC42 kinase 1
• UniProt Gene Name: TNK2
• UniProt Synonym Gene Names: ACK1; ACK-1
• UniProt Entry Name: ACK1_HUMAN