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WB (Western Blot)
(ACK1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).)
Rabbit ACK1 Polyclonal Antibody | anti-TNK2 antibody
ACK1 Antibody (C-term)
Gene Names
TNK2; ACK; ACK1; ACK-1; p21cdc42Hs
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ACK1, Antibody; ACK1 Antibody (C-term); Activated CDC42 kinase 1; ACK-1; Tyrosine kinase non-receptor protein 2; TNK2; ACK1; anti-TNK2 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 934-964 amino acids from the C-terminal region of human ACK1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
934-964
Sequence Length
1086
Applicable Applications for anti-TNK2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(ACK1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).)
WB (Western Blot)
(ACK1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).)
WB (Western Blot)
(Western blot analysis of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).)
WB (Western Blot)
(Western blot analysis of anti-ACK1 Antibody (C-term) in CEM cell line lysates (35ug/lane). ACK1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-ACK1 Antibody (C-term) in CEM cell line lysates (35ug/lane). ACK1(arrow) was detected using the purified Pab.)
Related Product Information for anti-TNK2 antibody
ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.
Product Categories/Family for anti-TNK2 antibody
References
Yokoyama, N., et al., J. Biol. Chem. 278(48):47713-47723 (2003).
Manser, E., et al., Nature 363(6427):364-367 (1993).
Manser, E., et al., Nature 363(6427):364-367 (1993).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114569
NCBI Official Full Name
activated CDC42 kinase 1 isoform 2
NCBI Official Synonym Full Names
tyrosine kinase, non-receptor, 2
NCBI Official Symbol
TNK2
NCBI Official Synonym Symbols
ACK; ACK1; ACK-1; p21cdc42Hs
NCBI Protein Information
activated CDC42 kinase 1
UniProt Protein Name
Activated CDC42 kinase 1
UniProt Gene Name
TNK2
UniProt Synonym Gene Names
ACK1; ACK-1
UniProt Entry Name
ACK1_HUMAN
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Product Notes
The TNK2 tnk2 (Catalog #AAA286043) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 934-964. The ACK1 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's ACK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TNK2 tnk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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