Rabbit ACK1 (Phospho-Tyr284) Polyclonal Antibody | anti-TNK2 antibody

ACK1 (Phospho-Tyr284) Antibody

Cat. Num. AAA290076

• Gene Names: TNK2; ACK; ACK1; ACK-1; p21cdc42Hs
• Reactivity: Human, mouse, rat
• Applications: Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: ACK1 (Phospho-Tyr284), Antibody; ACK1 (Phospho-Tyr284) Antibody; Activated CDC42 kinase 1; ACK-1; Tyrosine kinase non-receptor protein 2; TNK2; ACK1; anti-TNK2 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1,086

• Applicable Applications for anti-TNK2 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: synthetic peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) antibody.)

IHC (Immunohiostchemistry)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody.)

WB (Western Blot)

(Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using ACK1 (Phospho-Tyr284) antibody.)

Related Product Information for anti-TNK2 antibody

Non-receptor tyrosine-protein and serine/threonine- protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr- 287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.

Product Categories/Family for anti-TNK2 antibody

Phospho Antibodies

References

Manser E.,et al.Nature 363:364-367(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Eisenmann K.M.,et al.Nat. Cell Biol. 1:507-513(1999).
Kato J.,et al.Biochem. Biophys. Res. Commun. 268:141-147(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 58331191
• NCBI GeneID: 10188
• NCBI Accession #: NP_001010938.1
• NCBI GenBank Nucleotide #: NM_001010938.1
• UniProt Accession #: Q07912; Q6ZMQ0; Q8N6U7; Q96H59
• Molecular Weight: 114569
• NCBI Official Full Name: activated CDC42 kinase 1 isoform 2
• NCBI Official Synonym Full Names: tyrosine kinase, non-receptor, 2
• NCBI Official Symbol: TNK2
• NCBI Official Synonym Symbols: ACK; ACK1; ACK-1; p21cdc42Hs
• NCBI Protein Information: activated CDC42 kinase 1
• UniProt Protein Name: Activated CDC42 kinase 1
• UniProt Gene Name: TNK2
• UniProt Synonym Gene Names: ACK1; ACK-1
• UniProt Entry Name: ACK1_HUMAN