WB (Western Blot)
(Western blot analysis of extracts from 293 cells (Lane 2) and K562 cells (Lane 3), using ACK1 (Ab-284) antiobdy (AAA303072). The lane on the left is treated with synthesized peptide.)
Rabbit anti-Human, Mouse ACK1 Polyclonal Antibody | anti-ACK1 antibody
ACK1 (Ab-284) Antibody
Gene Names
TNK2; ACK; ACK1; ACK-1; p21cdc42Hs
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ACK1, Antibody; ACK1 (Ab-284) Antibody; Activated CDC42 kinase 1; ACK-1; EC=2.7.10.2; EC=2.7.11.1; Tyrosine kinase non-receptor protein 2; anti-ACK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ACK1protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
1086
Applicable Applications for anti-ACK1 antibody
WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from Internal of human ACK1.
Target Name
ACK1
Preparation and Storage
Store at -20°C.
WB (Western Blot)
(Western blot analysis of extracts from 293 cells (Lane 2) and K562 cells (Lane 3), using ACK1 (Ab-284) antiobdy (AAA303072). The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells (Lane 2) and K562 cells (Lane 3), using ACK1 (Ab-284) antiobdy (AAA303072). The lane on the left is treated with synthesized peptide.)
Related Product Information for anti-ACK1 antibody
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR.Manser E., Nature 363:364-367(1993) [PubMed: 8497321].Ota T.,Nat. Genet. 36:40-45(2004) [PubMed: 14702039].Muzny D.M., Nature 440:1194-1198(2006) [PubMed: 16641997].
Product Categories/Family for anti-ACK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
SDS-PAGE||115kd
NCBI Official Full Name
activated CDC42 kinase 1 isoform 2
NCBI Official Synonym Full Names
tyrosine kinase, non-receptor, 2
NCBI Official Symbol
TNK2
NCBI Official Synonym Symbols
ACK; ACK1; ACK-1; p21cdc42Hs
NCBI Protein Information
activated CDC42 kinase 1
UniProt Protein Name
Activated CDC42 kinase 1
UniProt Gene Name
TNK2
UniProt Synonym Gene Names
ACK1; ACK-1
UniProt Entry Name
ACK1_HUMAN
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Product Notes
The ACK1 tnk2 (Catalog #AAA303072) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACK1 (Ab-284) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ACK1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the ACK1 tnk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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