Rabbit Actin Polyclonal Antibody | anti-ACTB antibody

Actin (Tyr-53), Phospho-Specific

Cat. Num. AAA71548

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Human, Rat, Mouse, Chicken
• Applications: Immunocytochemistry, ELISA, Western Blot
• Purity: Affinity Purified
• Synonyms: Actin, Antibody; Actin (Tyr-53), Phospho-Specific; anti-ACTB antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken
• Clonality: Polyclonal
• Specificity: This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.; ; All molecular weights (MW) are confirmed by comparison to MW standards and to western blot mobilites of known proteins with similar MW. "Native" western blots utilizes non-reducing sample buffer (no mercaptoethanol or SDS), normal SDS-PAGE gel electrophoresis, and no methanol in transfer buffers.
• Purity/Purification: Affinity Purified
• Sequence Length: 375

• Applicable Applications for anti-ACTB antibody: ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Buffer: Rabbit polyclonal, affinity-purified antibody is supplied in 100 uL phosphate buffered saline, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.
• Immunogen: Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human β actin. This sequence is identical to similar regions in all four α actins, as well as in γ actin, and is well conserved in actins from most eukaryotic species.
• Preparation and Storage: Store at -20°C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide or phospho-Actin (Tyr-53) peptide, respectively (right).)

WB (Western Blot)

(Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).)

Related Product Information for anti-ACTB antibody

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four alpha isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (beta and gamma). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

References

Vonach, C. et al. (2011) British J Cancer. 105:263. Jungbluth, A. et al. (1995) FEBS Let. 375:87.Baba, T. et al. (2003) J. Immunol. 170: 3762.Winder, S.J. et al. (2005) J. Cell Sci. 118:651.Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694.

NCBI and Uniprot Product Information

• NCBI GI #: 4501885
• NCBI GeneID: 60
• NCBI Accession #: NP_001092.1
• NCBI GenBank Nucleotide #: NM_001101.5
• UniProt Accession #: P60709
• NCBI Official Full Name: actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1
• UniProt Protein Name: Actin, cytoplasmic 1
• UniProt Gene Name: ACTB
• UniProt Entry Name: ACTB_HUMAN