IHC (Immunohiostchemistry)
(Alpha-1-Antichymotrypsin Antibody-Anti-SERPINA3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
Rabbit anti-Human Alpha-1-Antichymotrypsin Polyclonal Antibody | anti-SERPINA3 antibody
Alpha-1-Antichymotrypsin Rabbit anti-Human Polyclonal (aa254-303) Antibody
Gene Names
SERPINA3; ACT; AACT; GIG24; GIG25
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunoaffinity purified
Synonyms
Alpha-1-Antichymotrypsin, Antibody; Alpha-1-Antichymotrypsin Rabbit anti-Human Polyclonal (aa254-303) Antibody; PathPlus Alpha-1-Antichymotrypsin Antibody (aa254-303); SERPINA3; Alpha-1-antichymotrypsin; AACT; GIG25; Growth-inhibiting protein 25; GIG24; Serpin A3; Growth-inhibiting protein 24; a-1-ACT; anti-SERPINA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human SERPINA3 isoform 1
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.09% sodium azide, 2% sucrose
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-SERPINA3 antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Target
Human Alpha-1-Antichymotrypsin
Immunogen
Synthetic peptide located between aa254-303 of human SERPINA3 (P01011, NP_001076). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Rat, Hamster (100%); Galago, Marmoset (84%).
Conjugation
Unconjugated
Epitope
aa254-303
Preparation and Storage
Short term: Store at 2-8 degree C. Long term: Aliquot and store at -20 degree C. Avoid freeze/thaw cycles.
IHC (Immunohiostchemistry)
(Alpha-1-Antichymotrypsin Antibody-Anti-SERPINA3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
IHC (Immunohiostchemistry)
(Alpha-1-Antichymotrypsin Antibody-Anti-SERPINA3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
WB (Western Blot)
(Alpha-1-Antichymotrypsin Antibody-721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
WB (Western Blot)
(Alpha-1-Antichymotrypsin Antibody-721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.)
Related Product Information for anti-SERPINA3 antibody
Alpha-1-Antichymotrypsin antibody is an unconjugated rabbit polyclonal antibody to Alpha-1-Antichymotrypsin (aa254-303) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Cited in 1 publication.
SERPINA3 (Alpha-1-Antichymotrypsin) is an alpha globulin glycoprotein produced in the liver that functions as a plasma serine protease inhibitor. It functions in tissue repair and helps reduce damage resulting from inflammatory proteolysis. It is also involved in regulating proliferation and chromatin condensation. Mutations in SERPINA3 are associated with Alzheimer's disease, Parkinson's disease and chronic obstructive pulmonary disease. Deficiency is correlated with liver disease, while upregulation of SERPINA3 potentially plays a role in the progression of prion diseases. Upregulated SERPINA3 is also associated with invasive properties and progression in glioblastoma. SERPINA3 is an inflammatory marker, and in immunohistochemistry of normal tissue it is positive in the cytoplasm and in extracellular deposits in histiocytes and reticulum cells. In cancer, it is positive in glioblastoma, acinar cell carcinomas of the pancreas, salivary gland and breast, and hepatoid adenocarcinomas of the stomach.
SERPINA3 (Alpha-1-Antichymotrypsin) is an alpha globulin glycoprotein produced in the liver that functions as a plasma serine protease inhibitor. It functions in tissue repair and helps reduce damage resulting from inflammatory proteolysis. It is also involved in regulating proliferation and chromatin condensation. Mutations in SERPINA3 are associated with Alzheimer's disease, Parkinson's disease and chronic obstructive pulmonary disease. Deficiency is correlated with liver disease, while upregulation of SERPINA3 potentially plays a role in the progression of prion diseases. Upregulated SERPINA3 is also associated with invasive properties and progression in glioblastoma. SERPINA3 is an inflammatory marker, and in immunohistochemistry of normal tissue it is positive in the cytoplasm and in extracellular deposits in histiocytes and reticulum cells. In cancer, it is positive in glioblastoma, acinar cell carcinomas of the pancreas, salivary gland and breast, and hepatoid adenocarcinomas of the stomach.
References
Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID: 26553804; Scientific Reports. 2017. 7 (15637), DOI: 10.1038/s41598-017-15778-8; Gastroenterology 2013;144:818, PMID: 23295442; Australian & New Zealand Journal of Psychiatry. 2014. Vol. 48(8): 722 -734, DOI: 10.1177/0004867414531078; Oncol Lett. 2018 Jan; 15(1): 285 -291, PMID: 29399139
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64,000
NCBI Official Full Name
alpha-1-antichymotrypsin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
NCBI Official Symbol
SERPINA3
NCBI Official Synonym Symbols
ACT; AACT; GIG24; GIG25
NCBI Protein Information
alpha-1-antichymotrypsin; serpin A3; growth-inhibiting protein 24; growth-inhibiting protein 25; cell growth-inhibiting gene 24/25 protein; serine (or cysteine) proteinase inhibitor, clade A, member 3
UniProt Protein Name
Alpha-1-antichymotrypsin
UniProt Gene Name
SERPINA3
UniProt Synonym Gene Names
AACT; ACT
UniProt Entry Name
AACT_HUMAN
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Product Notes
The SERPINA3 serpina3 (Catalog #AAA162544) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Alpha-1-Antichymotrypsin Rabbit anti-Human Polyclonal (aa254-303) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-1-Antichymotrypsin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the SERPINA3 serpina3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-1-Antichymotrypsin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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