Rabbit ATG4D Polyclonal Antibody | anti-ATG4D antibody

ATG4D Antibody

Cat. Num. AAA40561

• Gene Names: ATG4D; APG4D; AUTL4; APG4-D
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: ATG4D antibody is affinity chromatography purified via peptide column.
• Synonyms: ATG4D, Antibody; ATG4D Antibody; Autophagy related 4D cysteine peptidase; APG4D; APG4-D; AUTL4; autophagy related 4D, cysteine peptidase; anti-ATG4D antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATG4D antibody is human, mouse and rat reactive. At least two isoforms of ATG4D are known to exist; this antibody will detect only the larger isoform. ATG4D is predicted to not cross-react with other ATG4 proteins.
• Purity/Purification: ATG4D antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 474

• Applicable Applications for anti-ATG4D antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: ATG4D antibody was raised against a 17 amino acid peptide near the amino terminus of human ATG4D.
• Buffer: ATG4D antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ATG4D antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

IF (Immunofluorescence)

(Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 μg/ml.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 μg/ml.)

WB (Western Blot)

(Western blot analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 μg/ml.)

Related Product Information for anti-ATG4D antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4D, also known as AUTL4, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4).

NCBI and Uniprot Product Information

• NCBI GI #: 27903825
• NCBI GeneID: 84971
• NCBI Accession #: NP_116274
• NCBI GenBank Nucleotide #: NM_032885.5
• UniProt Accession #: Q86TL0; Q969K0
• Molecular Weight: Predicted: 52 kDa; ; Observed: 49 kDa
• NCBI Official Full Name: cysteine protease ATG4D isoform 1
• NCBI Official Synonym Full Names: autophagy related 4D, cysteine peptidase
• NCBI Official Symbol: ATG4D
• NCBI Official Synonym Symbols: APG4D; AUTL4; APG4-D
• NCBI Protein Information: cysteine protease ATG4D; autophagin-4; APG4 autophagy 4 homolog D; AUT-like 4 cysteine endopeptidase; ATG4 autophagy related 4 homolog D; autophagy-related protein 4 homolog D; cysteine protease involved in autophagy; autophagy-related cysteine endopeptidase 4
• UniProt Protein Name: Cysteine protease ATG4D
• UniProt Gene Name: ATG4D
• UniProt Synonym Gene Names: APG4D; AUTL4
• UniProt Entry Name: ATG4D_HUMAN