Rabbit ATG4D Polyclonal Antibody | anti-ATG4D antibody

Anti-ATG4D Antibody

Cat. Num. AAA293019

• Gene Names: ATG4D; APG4D; AUTL4; APG4-D
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: ATG4D, Antibody; Anti-ATG4D Antibody; APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D; anti-ATG4D antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.

• Applicable Applications for anti-ATG4D antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Function: [Cysteine protease ATG4D]: Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (PubMed:21177865, PubMed:29458288, PubMed:30661429). The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine (PubMed:21177865). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (By similarity). In addition to the protease activity, also mediates delipidation of ATG8 family proteins (PubMed: ,PubMed: ). Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy (PubMed: , PubMed: ). Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, by catalyzing delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989). ATG4D plays a role in the autophagy-mediated neuronal homeostasis in the central nervous system (By similarity). Compared to other members of the family (ATG4A, ATG4B or ATG4C), constitutes the major protein for the delipidation activity, while it promotes weak proteolytic activation of ATG8 proteins (By similarity). Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore- endoplasmic reticulum contacts during the lipid transfer phase of mitophagy (PubMed: ).
• Cellular Location: [Cysteine protease ATG4D]: Cytoplasm
• Tissue Location: Widely expressed in testis.
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt

IHC (Immunohiostchemistry)

(Dilution: WB: (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/100)Immunohistochemical analysis of ATG4D staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot)

(Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/100)Western blot analysis of ATG4D expression in Hela (A), A549 (B), PC12 (C), CT26 (D) whole cell lysates.)

Related Product Information for anti-ATG4D antibody

Rabbit polyclonal antibody to ATG4D
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary.

NCBI and Uniprot Product Information

• NCBI GI #: 27903825
• NCBI GeneID: 84971
• NCBI Accession #: NP_116274.3
• NCBI GenBank Nucleotide #: NM_032885.4
• UniProt Accession #: Q86TL0
• Molecular Weight: 52,922
• NCBI Official Full Name: cysteine protease ATG4D
• NCBI Official Synonym Full Names: autophagy related 4D, cysteine peptidase
• NCBI Official Symbol: ATG4D
• NCBI Official Synonym Symbols: APG4D; AUTL4; APG4-D
• NCBI Protein Information: cysteine protease ATG4D; autophagin-4; APG4 autophagy 4 homolog D; AUT-like 4 cysteine endopeptidase; ATG4 autophagy related 4 homolog D; AUT-like 4, cysteine endopeptidase; autophagy-related protein 4 homolog D; cysteine protease involved in autophagy; a
• UniProt Protein Name: Cysteine protease ATG4D
• UniProt Gene Name: ATG4D
• UniProt Synonym Gene Names: APG4D; AUTL4
• UniProt Entry Name: ATG4D_HUMAN