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IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit Bcl-2 Polyclonal Antibody | anti-Bcl-2 antibody
(KO Validated) Bcl-2 Polyclonal Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
Bcl-2; Polyclonal Antibody; (KO Validated) Bcl-2 Polyclonal Antibody; BCL2; PPP1R50; apoptosis regulator Bcl-2; anti-Bcl-2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-Bcl-2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
A synthetic peptide of human Bcl-2
Research Area
Cancer, Cell Biology, Metabolism, Signal Transduction
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human thyroid cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human thyroid cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and Bcl-2 knockout (KO) 293T cells using Bcl-2 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and Bcl-2 knockout (KO) 293T cells using Bcl-2 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines using Bcl-2 Polyclonal Antibody at 1:500 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines using Bcl-2 Polyclonal Antibody at 1:500 dilution.)
Related Product Information for anti-Bcl-2 antibody
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-Bcl-2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,266 Da
NCBI Official Full Name
apoptosis regulator Bcl-2 alpha isoform
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 2
NCBI Official Symbol
BCL2
NCBI Official Synonym Symbols
Bcl-2; PPP1R50
NCBI Protein Information
apoptosis regulator Bcl-2; protein phosphatase 1, regulatory subunit 50
UniProt Protein Name
Apoptosis regulator Bcl-2
UniProt Gene Name
BCL2
UniProt Entry Name
BCL2_HUMAN
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Product Notes
The Bcl-2 bcl2 (Catalog #AAA22320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (KO Validated) Bcl-2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the Bcl-2 bcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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