FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A431 cells using anti-BUB3 antibody (AAA126287).Overlay histogram showing A431 cells stained with AAA126287 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BUB3 Antibody (AAA126287, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human, Rat BUB3 Polyclonal Antibody | anti-BUB3 antibody
Anti-BUB3 Antibody Picoband
Gene Names
BUB3; BUB3L; hBUB3
Reactivity
Human, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
BUB3, Antibody; Anti-BUB3 Antibody Picoband; anti-BUB3 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-BUB3 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human BUB3 recombinant protein (Position: R50-D209).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A431 cells using anti-BUB3 antibody (AAA126287).Overlay histogram showing A431 cells stained with AAA126287 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BUB3 Antibody (AAA126287, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A431 cells using anti-BUB3 antibody (AAA126287).Overlay histogram showing A431 cells stained with AAA126287 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BUB3 Antibody (AAA126287, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of BUB3 using anti-BUB3 antibody (AAA126287).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: rat thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB3 antigen affinity purified polyclonal antibody (#AAA126287) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BUB3 at approximately 40 kDa. The expected band size for BUB3 is at 37 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of BUB3 using anti-BUB3 antibody (AAA126287).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: rat thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB3 antigen affinity purified polyclonal antibody (#AAA126287) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BUB3 at approximately 40 kDa. The expected band size for BUB3 is at 37 kDa.)
Related Product Information for anti-BUB3 antibody
BUB3(BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene. And it is mapped on 10q26.13. Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint(SAC); though BUB3 is non-essential in yeast, it is essential in higher eukaryotes. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation. Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC. Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2. Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.
Product Categories/Family for anti-BUB3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,155 Da
NCBI Official Full Name
mitotic checkpoint protein BUB3 isoform b
NCBI Official Synonym Full Names
BUB3 mitotic checkpoint protein
NCBI Official Symbol
BUB3
NCBI Official Synonym Symbols
BUB3L; hBUB3
NCBI Protein Information
mitotic checkpoint protein BUB3; mitotic checkpoint component; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog
UniProt Protein Name
Mitotic checkpoint protein BUB3
UniProt Gene Name
BUB3
UniProt Entry Name
BUB3_HUMAN
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Product Notes
The BUB3 bub3 (Catalog #AAA126287) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BUB3 Antibody Picoband reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BUB3 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the BUB3 bub3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BUB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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